AIM: To observe the effect of curcumin on the retinal structure and the expressions of interleukin-23 (IL-23) and interleukin-17(IL-17) in the rat retina after retinal ischemia-reperfusion injury (RIRI).METHODS:A total of 60 male Sprague-Dawley(SD) rats were randomly divided into normal control group(NCG),model group(MG),low-dose curcumin group (LDCG) and high-dose curcumin group (HDCG) (n=15 per group).RIRI was generated by anterior chamber perfusion of normal saline to the right eye.Rats in LDCG and HDCG received an intraperitoneal injection of 20mg/kg/d and 100mg/kg/d curcumin respectively,at 30min before RIRI and once daily after RIRI.Retinal structure and inflammation were evaluated after hematoxylin and eosin-stained (HE) staining.Western-blot and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-23 and IL-17 expressions after RIRI.RESULTS: The retinal structure of NCG was normal.Retinal edema,empty spaces or loosely packed cells and inflammatory cell infiltration were observed in MG and LDCG groups,whereas the morphological changes in HDCG group were improved as compared to MG and LDCG groups.Western-blot assay and ELISA showed that IL-23 and IL-17 expressions increased significantly after RIRI (vs NCG,P<0.01).Moreover,curcumin reduced IL-23 and IL-17 expressions significantly (vs MG,P<0.01).CONCLUSION: Curcumin can inhibit leukocytes infiltration and improve the retinal pathologic changes.Furthermore,curcumin can reduce retinal IL-23 and IL-17 expressions significantly in a dose-dependent manner.

?AIM:To investigate and analyze the prevalence and the influencing factors on retinal arteriosclerosis in civil aviators of different ages.?METHODS:Two thousand seven hundred and seventy-eight commissioned civil aviators who received medical examination in Civil Aviation General Hospital from July 2014 to July 2015 were randomly divided into three groups: group A ( 1 311 aviators aged 22-39 ) , group B (618 aviators age 40-49 ), group C(849 aviators age 50-59). Aviators over 40 years old, were divided into two groups: group with retinal atherosclerosis and group without retinal atherosclerosis according to the results of fundus examination. Medical examination data of the aviators were analyzed.?RESULTS: Of 2 778 aviators, 334 ( 12. 02%) were detected with retinal arteriosclerosis, among which 318 aviators were classified as gradeⅠ, and 16 aviators as grade Ⅱ. The retinal arteriosclerosis prevalence of group A, B, and C increased with age, and the difference was statistically significant ( P < 0. 05 ). Biochemical tests showed that the prevalence of hypertension, hyperlipidemia, high fasting blood glucose and overweight increased in group with retinal arteriosclerosis compared with that in group without retinal arteriosclerosis, and the difference was statistically significant(P<0. 05).?CONCLUSION:The prevalence of retinal arteriosclerosis in commissioned civil aviators was 12. 02%, and most of the retinal arteriosclerosis was classified as grade Ⅰ. In aviators over 40 years old, hypertension, hyperlipidemia, high fasting blood glucose and overweight were important factors in the development of retinal arteriosclerosis.

Objective To seek a operation method of quality control of biochemical tests in peacekeeping mission via perspective of evidence-based laboratory medicine .Methods Literatures related to quality control of biochemical tests in international peace-keeping were referred ,compared and discriminated .Combined with 5-year peacekeeping laboratory experience ,a set of feasible ap-proaches were worked out and a practice evaluation was performed in a task period .Results Under the supervision of the quality control system ,providing accurate and reliable test results to the clinical ,developing and implementing quality control of biochemical tests were economical ,practical and reliable ,and could guarantee the effectiveness of reports of biochemical test in peacekeeping medical institutions .Conclusion Evidence-based laboratory medicine is important for quality control of biochemical tests in peace-keeping medical institutions .

ObjectiveTo observe the clinical therapeutic effect of intra-articular injection with sodium hyaluronate (SH) on osteoarthritis of the ankle.Methods25 patients with osteoarthritis of the ankles (36 ankles) were treated with SH intra-articular injection 2 ml every week for 5 weeks. The degree of pain, swelling and functional disturbance of the ankles were observed to evaluate the effect.ResultsAfter treatment, the degree of pain, swelling and functional disturbance of the ankles improved (P<0.05～0.01). The total efficiency rate was 91.61%.ConclusionSodium hyaluronate injected intra-articular is an effective, ideal and safe treatment for osteoarthritis of the ankle.

Objective To explore the application of magnetic resonance angiography and virtual reality technique in preoperative planning for sphenoid ridge meningioma. Methods Ten patients with sphenoid ridge meningiomas were examined by brain CT, MRI and MRA before operation. Then the image data were transferred to the Dextroscope system. The images were reconstructed using the Radiodexter software to display the three dimensional image. The tumor and its adjacent anatomic structures could be viewed and the system tools allowed for simulation of the operation, so the full information of the tumor, basilar texture, and nerves and vessels could be obtained. A pre-operative planning was done and was compared with intra- operation situation. Results The virtual reality images of the 10 cases were successfully conducted, and all the procedures were performed according to the pre-operative planning, with a consistent rate of 100%. Radical resection was achieved and all the 10 patients and there were no sequelea. Conclusion Magnetic resonance angiography and the virtual reality system can provide the information of tumor and its adjacent structures, so it can Hp the neurosurgeons no planning the details for operation, contributing to operation safety and tumor resection.

Adult ; Humans ; Male ; Mediastinitis ; complications ; Neck ; Peritonsillar Abscess ; complications

Acute Disease ; Adolescent ; Adult ; Aged ; Child ; Female ; Follow-Up Studies ; Humans ; Ivermectin ; analogs & derivatives ; poisoning ; Male ; Middle Aged ; Retrospective Studies ; Young Adult

Objective To investigate oxycodone hydrochloride injectio in treatment of postoperative laparoscopic cholecystectomy and its effects on analgesia and levels of 5-hydroxytryptamine(5-HT),nitric oxide(NO),substance P(SP).Methods 90 patients of underwent laparoscopic cholecystectomy who received therapy from August 2013 to August 2016 in Hangzhou Tvaditional chinese Medical hospital were selected and randomly divided into observation group and control group,45 cases in each group.Before incision,the observation group were gived intravenous oxycodone hydrochloride injection 0.1mg/kg,the control group were gived intravenous sufentanil 0.2μg/kg.The changes of anesthesia,postoperative pain,adverse reactions and 5-HT,NO and SP after operation were compared between the two groups.ResultsThere was no significant difference in the anesthesia time,operation time,spontaneous breathing recovery time,wake up of time between two groups,after postoperative two,four,24 h,the scores of incision pain,visceral pain in the observation group were lower than the control group,the difference was statistically significant(P<0.05).After the operation,the levels of 5-HT,NO,SP of two groups were changed,the difference was statistically significant(P<0.05),but the results in observation group were lower than the control group,the difference was statistically significant(P<0.05),the total incidence of postoperative adverse reactions in observation group were lower than the control group,the difference was statistically significant(P<0.05).Conclusion Oxycodone hydrochloride injection is well for laparoscopic cholecystectomy,which can effectively reduce the release of 5-HT,NO,SP,relief of postoperative pain,high security.

Objective To establish a method for the determination of Baicalin in Fuke Yangkun pills.Method High-performance liquid chromatography method was used.The chromatographic conditions were as follows:C18 column,mobile phase of methanol ∶0.4 %phosphoric acid(45 ∶55),detection wavelength being 280 nm,a flow rate of 1.20 mL?min-1 and the column temperature being 25 ℃.Results There existed a good linearity when the injection quantity is in the range of 0.0541 ?g to 0.5408 ?g.Average recovery is 99.98 %,and RSD is 1.32 %.Conclusion HPLC method is simple and effective and can be applied for the detemination of Baicalin in Fuke Yangkun pills.

Objective To investigate the diagnosis and treatment for biliary papillomatosis. Methods 23 patients who were pathologically diagnosed as biliary papillomatosis and treated in our hospital from Jan. 2003 to Mar. 2008 were enrolled in this study. Their ages, genders, clinical manifestations, diagnostic approaches,histopathological findings, intraoperative findings, treatments and prognosis were analyzed retrospectively. Recal manifestations were abdominal pain (83.3%) and obstructive jaundice (72.2%). All the patients were diagnosed pathologically through intraoperative frozen section. No patient was diagnosed preoperatively. Anatomically, solitary lesion was observed in 10 patients (43.5%) and multiple lesions in 13 patients (56.5%), with a median tumor size of 2.6 cm. Microscopically, carcinomatous change was found in one patient, mild atypical hyperplasia in three, and medium atypical hyperplasia in one. Therapies mainly included radical excision and palliative external drainage. All but one patient recovered well postoperatively. This patient had anastomotic leak. All 23 patients were followed up with a mean period of 34 months. During the follow-up, three patients died as a consequence of biliary malignancy, one patient had the recurrence and others without recurrence. Conclusions Biliary papillomatosis is very difficult to diagnose preoperatively and the definitive diagnosis mainly depends on intraoperative and postoperative pathologic examination. To obtain a better prognosis, management should be aimed at radical excision. Intraoperative cholangioscopic evaluation and frozen section are strongly recommended to guide the choice of appropriate therapeutic approach.