OBJECTIVE: To establish a method of GC-Second Order Temperature Programming for determining the residual quantities of 4 organic solvents-methanol, acetonitrile, pyridine and dimethylformamide-in Methylprednisolone Sodium Succinate. METHODS: The separation was performed on DB-5 capillary column, with flame ion monitor as detector, and with high pure nitrogen, air and hydrogen as carrier gas. The column temperature was subjected to GC-Second Order Temperature Programming, with the temperature of sample injector at 200℃ and that of detector at 220℃. The make-up gas was high pure nitrogen. The split ratio was 10∶1. The sample size was 2?L and the butanone was taken as the internal standard. RESULTS: The four kinds of solvents were linear within their respective concentration range. The average recoveries of methanol, acetonitrile, pyridine and dimethylformamide were 99.4%, 99.2%, 99.3% and 99.0%, respectively, with RSD at 1.8%, 1.8%, 2.0% and 1.7%, respectively. CONCLUSION: The method was proved to be sensitive, accurate and simple.

OBJECTIVE: To establish a auantitative analysis of multi-components by single marker(QAMS) method for determining the content of anthraquinones in Dabaidu capsules(traditional Chinese medicines). METHODS: The relative correction factors (RCFs) of emodin to rhein, chrysophanol, and physcion were determined on a C18 column (4.6 mm × 250 mm, 5μm) with methanol-0.1% phosphoric acid (85:15) as mobile phase. The flow rate was 1.0 mL · min-1. The column temperature was set at 25°C. The detection wavelength was set at 254 nm. The relative retention time (RRT) was used to define the target peak. RESULTS: The calibration curves for rhein, emodin, chrysophanol, and physcion showed good linearity in the ranges of 5.724 × 10-2-0.5724 μg, 4.584 × 10-2 -0.4584 μg, 6.368 × 10-2-0.6368 μg and 6.548 × 10-2 - 0.6548 μg, r(0.9997-0.9999), respectively. The recoveries were (97.8 ± 1.4)%, (100.7 ± 1.0)%, (98.6 ± 0.6)% and (97.0 ± 1.4)%, respectively. The RCFs were 1.19, 1.40 and 1.06. CONCLUSION: The method is simple, rapid, accurate and reliable. It can be applied to control the quality of anthraquinones in Dabaidu capsules.

A kind of magnesium potassium phosphate cement developed for bone repair was cleared by FDA in 2009 and has been presently in clinical use in America.The biomaterial has the powerful adhesive capability to bind bone,ligament,and tendon to bone,as well as possessing good biocompatiblity,appropriate biodegradability and osteogenicity; to data,it is the only material which possesses the combination of adhesivity and osteogenicity among bone repair biomaterials.The clinical application of the innovative biomaterial will unprecedentedly alter the treatment in orthopedic surgery and related disciplines.To provide a comprehensive appreciation of the innovative biomaterial,this article summaries its development,characteristics of composition and preparation,formation mechanism,application study and superiority.

Congenital Hypothyroidism ; diagnosis ; Diagnostic Errors ; Humans ; Infant, Newborn ; Male ; Water-Electrolyte Imbalance ; complications

Chromosome Disorders ; Chromosomes, Human, Pair 13 ; Humans ; Infant ; Male ; Trisomy ; Trisomy 13 Syndrome

Objective To investigate the relationship of pediatric obstructive sleep apnea hypopnea syndrome (OSAHS) with different body mass mdex(BMI) and the severity of OSAHS. Methods Nine children with high BMI, 10 children with low BMI and 25 children with normal BMI which were monitored and diagnosed OSAHS by nocturnal polysonmography(PSG ) ,and the parameters of PSG were analyzed. Results The high BMI group findings were significantly higher AHI(21.61 ? 7.42 vs 11.32?4.16 P

Objective To observe the efficiency and safety of Extecavir on chronic severe hepatitis B.Methods 36 patients with chronic severe hepatitis B were randomly divided into 2 groups. Control group (n=16) was given the conventional therapy. Treatment group (n=20) was treated with Entecavir, 0.5mg daily for four weeks, on the bases of routine treatment. Before and after treatment, hepatic function, prothrombin active (PTA), HBVDNA and efficiency rate of the two groups were detected.Results the results showed that the total efficiency rates of treatment group and control group were 85% and 50% respectively. There was significant difference between the two groups (P＜0.01). After treated for four weeks, the improvement of TBil and PTA effect was observed.Conclusion It can be concluded that Entecavir has good therapeutic efficiency and safety in the treatment of chronic severe hepatitis B.

Objective: To develop an expeditious method for the simultaneous determination of main active compounds in Fufang Ejiao Syrup (FES, compound Ejiao oral liquid) by near infrared spectroscopy (NIRS) in transflective mode. Methods: The contents of total flavonoids and total saponins were determined by colorimetric assays, while the soluble solid content was determined using a moisture analyzer as reference value. Partial least squares regression (PLSR) was employed for the calibration of regression models between NIRS and the indexes. Results: The correlation coefficients (R2) of the constructed models for total flavonoids, total saponins, and soluble solid content were 0.9798, 0.9660, and 0.9978, respectively, and the root mean square errors of prediction (RMSEP) were 69.6, 17.6 μg/mL, and 0.394%, respectively. Conclusion: The proposed method is fast and accurate, and facilitating the rapid analysis of main active components in FES.

Hide melting presents itself as one of the most critical processes in the production of donkey-hide gelatin. Here a NIR-based method was established for the rapid analysis of in-process hide melting solutions as well as for end-point determination of this process. Near infrared (NIR) spectra of hide melting solutions were collected in transflective mode. With the contents of total nitrogen determined by the Kjeldahl method as reference values, partial least squares regression (PLSR) was employed to build calibration models between NIR spectra and total nitrogen. Model parameters including wavelength range and PLS factors were optimized to achieve best model performance. Based on the contents of total nitrogen predicted by calibration model, end point of hide melting was determined. The constructed PLS model gave a high correlation coefficient (R2) of 0.991 3 and a root mean square error of prediction (RMSEP) of 0.807 g x L(-1). With the predicted total nitrogen and predefined limit, decisions concerning the proper times of melting were made. This research demonstrated that NIR transflectance spectroscopy could be used to expeditiously determine the contents of total nitrogen which was subsequently chosen as the indictor for determining the end-point of hide melting. The proposed procedure may help avoid unnecessary raw material or energy consumption.
Animals ; Calibration ; Endpoint Determination ; methods ; Equidae ; anatomy & histology ; Gelatin ; chemistry ; Least-Squares Analysis ; Nitrogen ; analysis ; chemistry ; Skin ; chemistry ; Spectrophotometry, Infrared ; Time Factors ; Transition Temperature

Objective To study influences of magnetic field on behavior of depressive rats under chronic unpredictable mild stress,and possible mechanisms under such phenomena.Methods Thirty-six Wistar rats were divided into model group,magnetic field group and control group (n =12 in each group) according to random number table.The model group and the magnetic field group received CUMS stimulation daily for 5 weeks,while the control group received no any treatment.At the end of the fifth week,rats in the magnetic field group were placed in the center of a magnetic machine (400 mT),and the model group was raised freely for4 weeks.The severity of depressive behaviors,cortisol levels and contents of C-reactive protein in blood and urine sample were tested pre-treatment,at the end of 5 weeks and 9 weeks.Results After 5 weeks,consumption of glucose solution of the control group was higher than the other two groups (P ＜ 0.05).After 5 and 9 weeks,body weights of the magnetic group and the model group were lower than the control group (P ＜ 0.05).At the end of 9 weeks,performance of the magnetic group as indicated by frequency of grid crossing,grooming time and suspending tail test were improved to a significantly greater extent than the other two groups (P ＜ 0.05).In addition,the urine content of cortisol in 24 hours in magnetic field and model groups was higher than the control group at the end of 5 weeks,and the serum level of cortisol in the magnetic field group were significantly higher than the model group at the end of 9 weeks (P ＜ 0.05).Conclusion The 400mT magnetic field can reduce the severity of behavior changes of rats with CUMS-induced depression,which might be attributable to its influence on the cortisol levels in blood and urine.