Objective:To study the effect of lovastatin on the proliferation of rat mesangial cell(RMC) and the activity of TGF-?1 gene promoter.Methods:(1)Effect of lovastatin(10~(-7),10~(-6),10~(-5) and 10~(-4) mol/L) on proliferation of RMC was analyzed by MTT.(2)Two plasmids(phTGF2.14 and phTGF1.12) containing various lengths of 5′flank sequences of human TGF-?1 gene and CAT as reporter gene were constructed and transfected into RMC by FuGENE transfection reagent.The effects of lovastatin(10~(-7),10~(-5)mol/L)on transcription of the 2 plasmids were studied by CAT-ELISA.Results:(1)Different concentrations of lovastatin had obvious inhibitive effects on the proliferation of RMC compared with control group(P

Objective To observe the effect of self mading Tongfengxiao decoction in treatment of acute gouty arthritis. Methods 69 patients were randomly divided into two groups. In the control group,33 patients were given diclofenac sodium sustained-release capsules and allopurinol treatment;In treatment group ,36 patients were given to the self mading Tongfengxiao decoction in addition to the western medicine received by the control group. The two groups were all treated for 14 days. Results The total effective rate was 94.4% for treatment group and 87.8% for control group respectively. Therefore, the difference of treatment effect between twp groups was significant ( P ＜0. 05 ) ;Also, the treatment group performed better than control group in term of reducing serum uric acid( P ＜ 0. 05).Conclusion The treatment of Tongfengxiao decoction combined western medicine for acute gouty arthritis had a good synergistic effect, and could reduce serum uric acid and improve clinical symptoms.

Animal health systems initially established in Australia,Canada and other countries,played a very important role in prevention and control of animal diseases.Based on comparing the animal health systems of Australia with the veterinary medical system in China,some strategies was obtained for establishing a more reasonable official veterinary system and animal health system in China,thus speeding up the integration process of veterinary and human medical public health system.

Communicable Disease Control ; Global Health ; Humans ; Severe Acute Respiratory Syndrome ; prevention & control

Due to the completion of human genome project and the advent of new technologies, the traditional drug discovery is shifting to genomics-based drug discovery. Target discovery is an important first step in drug discovery pipeline. In this review, we critically introduce the strategies and biology technologies for target discovery, especially focus on the likely impact of new technologies in drug target research and speculate the future trend of target research.

Acupuncture ; education ; instrumentation ; methods ; Acupuncture Points ; Acupuncture Therapy ; instrumentation ; methods ; Humans ; Meridians ; Moxibustion ; instrumentation ; methods

Objective To observe the effects of berberine hydrochloride on the secretion of interleukin-6(IL-6)from human periodontal ligament cells(PDLCs)cultured in vitro.Methods Periodontal ligament tissues were isolated from extracted human premolars,and PDLCs were cultured in vitro.After identification by immunohistochemistry technique,human PDLCs were divided into LPS group and NLPS group according to whether lipopolysaccharide(LPS,50 ?g/mL)was contained in cell culture media.Then in accordance with the final concentrations of berberine hydrochloride in cell culture media(0,0.010,0.020,and 0.030 g/L),the groups were subdivided into LPS and NLPS control group,LPS1 and NLPS1 group,LPS2 and NLPS2 group,LPS3 and NLPS3 group.IL-6 contents in supernatants of each group were determined by enzyme-linked immunosorbent assay,and statistical analysis and comparison were conducted as well.Results Compared with corresponding LPS and NLPS groups,IL-6 contents in supernatants of LPS3 group and NLPS3 group reduced markedly(P

italic>Cannabis sativa, one of the ancient medicinal plants, has been used to alleviate pain and seizures. However, cannabinoids are often addictive, which limits their clinical use. Cannabidiol (CBD) as a non-psychoactive component of Cannabis sativa, has much weaker adverse effects than Δ9-tetrahydrocannabinol (THC) and therefore has received widespread attention. CBD has been found to ameliorate a variety of neuropsychiatric diseases, but the precise mechanism(s) of action are still unclear. Due to its low affinity for classical cannabinoid receptors current studies are focusing on other targets outside the endocannabinoid system. In the present review we mainly summarize the effects and molecular mechanisms of CBD in neuropsychiatric disorders, including epilepsy, neuropathic pain, anxiety, and depression.

Cardiovascular Diseases ; Humans ; Trans Fatty Acids

MicroRNA(miRNA)is a small non-coding RNA that contains 21 to 23 nucleotides and can down-regulate gene expression by translational repression.Recent studies found that some miRNAs might function both as oncogenes and tumor suppressors;its role in the tumorigenesis may complement and enrich the mechanisms of tumorigenesis.Liver cancer is a great threat to human health,whose pathogenesis is still not completely understood.MiRNAs can influence the tumorigenesis,differ- entiation and treatment of liver cancer.Here we summarizes the related progression in research of miRNA and liver cancer.