[Objective]To study the proliferation inhibition effect of decetaxel combined with cisplatin on osteosarcoma cells,and the effect of the drugs during the course of chemotherapy for osteosarcoma.[Method]The effect of docetaxel and cisplatin alone or combined with osteosarcoma cell line HOS-8603 was examined by cell count,morphologic observation,flow cytometry(FCM).The animal model of human osteosarcoma was established in nude mice.They were given docetaxel or cisplatin alone and combined,and a blank control group was set up.Observations on the growth and pathologic markers of osteosarcoma were carried out and pathological examinations were made.[Result]Docetaxel and cisplatin either alone or combined inhibited significantlythe proliferation of osteosarcoma cells and induced obviously apoptosis,and the effect of the drags combined was more effective than that of the drugs alone,Growth inhibition ratio ≥59.34%(P

OBJECTIVE:To provide the reference for drug stores to choose the chain mode:the direct sales or franchise.METHODS:The common modes of the drug store’chain administration and the current condition of the chain drug store industry in china were summarized;The merits&demerits of each mode and the environment,which influenced the choice of chain modes,were analyzed.RESULTS&CONCLUSION:The phenomenon can not be the proof that direct sales are better than the franchise in the market.The Chinese pharmaceutical chain enterprises should make their own choices based on the specific situation.The improved franchise mode is very competitive in the market.

OBJECTIVE:To provide suggestions for retail drugstores on how to tackle problems of non-quality induced drug returning from customers.METHODS:Reasons why it is hard to return the already purchased drugs were analyzed based on the status quo of drug returning in retail drugstores,and some countermeasures were presented on how to reduce or avoid non-quality induced drug returning problems.RESULTS&CONCLUSION:Non-quality induced drug returning problems can be well solved in drugstores through improving pharmaceutical care level and management level.

Cardiovascular Diseases ; diagnosis ; therapy ; Cerebrovascular Disorders ; diagnosis ; therapy ; Humans ; Medicine, Chinese Traditional ; methods

Objective: To summarize our experience on the anesthetic management, cardiopulmonary bypass, and protection of major organs total aortic arch replacement in patients with DeBakey type I aortic dissection. Methods: From June 2005 to June 2006, 5 patients with acute DeBakey type I aortic dissection received total aortic arch replacement under general anesthesia and deep low temperature. The 5 patients, including 3 man and 2 women, with an age range of 49-76 years, all received emergent operation. The clinical data were retrospectively analyzed and the experience on anesthesia management was summarized. Results: All the 5 patients had uneventful anesthesia with propofol. The time of cardiopulmonary bypass was 111-148 min, with a mean of (127.2±16.6) min. The priming solution was Ringer acetate solution. The selective cerebral perfusion time was 11-18 min, with a mean of (15.2±2.8) min. The lowest temperature was 19-22°C, with a mean of (20.4±1.5)°C. Modified pH-stat technique was employed for temperature lowing and the rate of rewarming was controlled strictly. The right axillary artery cannulation was routinely performed for cardiopulmonary bypass and selected cerebral perfusion. The blood transfusion was 600-1 400 ml. The auditory evoked potentials index was 0 during lower body arrest. There were no serious cerebral complications after operations. Four patients survived and one died of renal failure 6 days after operation. Conclusion: General anesthesia combined with deep low temperature and selected cerebral perfusion provides a safe condition for ascending arota and total arotic replacement.

Objective To use numerical simulation for analying hemodynamic changes in intracranial aneurysm with elastic wall, and to analyze the effect of mechanical properties of the aneurysm wall on the hemodynamic changes. Methods Based on the DSA images of a patient with multi-intracranial aneurysm, the numerical simulation of the hemodynamics of the elastic intracinal aneurysm was processed using a software of finite element method of computational fluid dynamics (CFD) and the computational fluid-structure interaction analyses. We also investigated the effect of the coupling of hemodynamics and aneurysm wall movement. Results The morphology of the elastic aneurysm underwent deformation, and the angle between aneurysm and parent artery also changed, affecting the inflow jet dispersed into the flow field of aneurysm and the distribution of wall shear stress on the surface of aneurysm. Conclusion The numerical simulation of CFD can directly reflect the hemodynamic characteristics of aneurysms. More accurate elastic wall aneurysm model is needed to improve the quantitative analysis of the hemodynamics of intracranial aneurysms.

The changes of morphology, ATP and malonaldehyde (MDA) contents, xanthine oxidase (XO) activity as well as the glutathione peroxidase (GSH-px) activity of rabbit aortic endothelial cells under hyperoxia (100% O_2) for 0-72 hours were studied. We found that cellular morphological changes including swelling, shape variation after hyperoxia were time-dependent; after a temporarily increasing (24hr)(P

Objective: To summarize our experience on anesthesia management of patients who suffered from cervical spine injury after earthquake. Methods: The anesthesia management data of the 6 patients suffering from cervical spine injury during Wenchuan Earthquake (May 12,2008,China) were retrospectively analyzed. Results: After antishock treatment and stabilization of vital signs, the 6 patients underwo nt surgery under general anesthesia in an open field hospital (intubation was conducted with slow induction) or in our hospital (intubation was conducted with bronchofibroscope). During operation the patients showed smooth respiratory and cardiovascular characteristics, and recovered well after operation. Conclusion: Under the field condition, intubation with slow induction is a good choice for patients with cervical spine injury; while in a hospital, intubation with bronchofibroscope should be chosen because it causes less movement of cervical spine.

Objective: To summarize our experience in perioperative anesthetic management for fulminant hepatic failure (FHF) patients receiving liver transplantation. Methods: The clinical anesthetic data of 48 FHF patients receiving orthotopic liver transplantations (OLT) from January 2006 to January 2007 were retrospectively analyzed, and the anesthetic management experience was summarized. General anesthesia was applied; the hemodynamics was monitored during the operation and doses of adrenaline and phenylephrine were adjusted according to the monitoring results. Blood samples were obtained before operation, before anheptic, 30 min after anhepatic P base, 5 min before neohepatic phase, and 5 min, 30 min and 60 min after neohepatic phase for blood gas and electrolyte analysis and for determination of coagulation function; the drugs were subsequently adjusted according to analysis results. Results: All the 48 patient underwent successful anesthetic management and there was no death during opearation. The average blood loss during operation was (5 219±478) ml. Mild alkalosis, hypokalemia, hyponatrium, and hypocalcemia were present before operations. pH, BE and HCO3-were obviously reduced 30 min after anhepatic phase and increased 60 min after neohepatic phase. Kalemia was obviously increased 30 min following anhepatic phase and began to increase 60 min following neohepatic phase. Calium concentration was decreased at the end of preanhepatic phase (P

Objective: To summarize our experience on anesthetic management for liver re-transplantation. Methods: The clinical data of 20 patients who received liver re-transplantation under general anesthesia were retrospectively analyzed. General anesthesia was given to all patients. Ambulatory blood pressure (ABP), electrocardiogram (ECG), central venous pressure (CVP), pulmonary artery pressure (PAP), pulmonary artery wedge pressure continuous cardiac output (CCO), cardiac index (CI), partial pressure of end-tidal carbon dioxide pressure (PetCO2), auditory evoked potentials index (AEPI), body temperature (T), and urine volume were continuously monitored during the operation. Pulse-induced contour cardiac output (PiCCO) technology was used to measure global end-diastolic volume (GEDV), intrathoracic blood volume (ITBV), extravascular lung water (EVLW), systemic vascular resistance (SVR), and stroke volume variation (SVV). The following data of patients, including the periods between the 2 operation, the presence of abdominal infection and multiple organ system failure (MOSF), the mode of re-operation, the operation duration, non-liver time, blood loss, blood transfusion, prothrombin time (PT), international normalized ratio (INR), preoperative creatinine, preoperative bilirubin, and the use of volven, albumin, 5% sodium bicarbonate, fibrinogen and thrombin, were all investigated and compared between the 2 operations. Results: All the 20 patients survived after liver re-transplantation. During the operation the hemodynarnic state, urine volume, electrolytes, and acid-base balance were all stable. The duration of the re-operation was significantly longer compared with that of the first operation (P<0.05), and the blood loss, blood transfusion, and the used of fibrinogen, thrombin and 5% sodium bicarbonate were all significantly more than those of the first operation (P<0.05). Conclusion: Anesthetic management for liver re-transplantation is very complicated; better understanding of patients condition and operation, careful observation during operation, and correct management in time are the keys for successful operation.