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OBJECTIVE:To observe effects and safety of Kangfuxin solution combined with intense pulsed light in the treat-ment of rosacea. METHODS:A total of 50 rosacea patients in our hospital during May 2014-Jun. 2016 were divided into control group(25 cases)and observation group(25 cases)according to random number table. Based on oral administration of Metronida-zole tablets,control group received intense pulsed light. Observation group was additionally given Kangfuxin solution for local wet compress after 4 to 6 layers of gauze saturated with liquid,5-10 min,qn. Both groups received treatment for 4 weeks. Clinical effi-cacies,as well as symptom score and DLQI score were compared between 2 groups before and after treatment,and the occurrence of ADR was recorded. RESULTS:The response rate of observation group was 92.0%,which was significantly higher than 64.0%of control group,with statistical significance (P<0.05). Before treatment,there was no statistical significance in erythema,pap-ules,pustules,itching,telangiectasia score and total score,DLQI score with before treatment(P>0.05). After treatment,erythe-ma,papules,pustules,itching,telangiectasia score and total score,DLQI score of 2 groups were decreased significantly,and the observation group was significantly lower than the control group,with statistical significance(P<0.05). The incidence of ADR in observation group was 16.0%,which was significantly lower than 40.0% in control group,with statistical significance(P<0.05). CONCLUSIONS:Kangfuxin solution combined with intense pulsed light show significant efficacy for rosacea,and can effectively improve erythema,papules,pustules,itching and telangiectasia,and improve the quality of life with good safety.

Abstract Biomarkers could improve the understanding of the causes of obesity and its association with chronic diseases for people. The purpose of the review is to summarize recent advances in transcriptomic, proteomic, and metabolomic phenotypic biomarkers of obesity in order to deepen the understanding of the etiology of obesity and its metabolic consequences. In the precise prevention and control of childhood obesity, different groups of biomarkers can improve the accuracy of the word "obesity" and help early detection of specific biomarkers with risk characteristics, so as to realize the transformation of childhood obesity from a one size fits all prevention and control strategy to a personalized prevention and control plan during the development of obesity.

Objective: To investigate the effect of Baohuoside-Ⅰ on the proliferation, invasion, migration and apoptosis of colon cancer cell lines SW480 and RKO and the relative mechanism. Methods: Colon cancer cell lines SW480 and RKO were respectively treated with different concentrations of Baohuoside-Ⅰ (0, 5, 10, 20 μg/ml). Cell proliferation was detected by MTT assay; The ability of cell clone formation was tested by cell clone formation experiments; The migration and invasion of cells were detected by Transwell assay; The apoptosis and cell cycle was detected by Flow cytometry; and the protein expression levels of cleaved PARP, cleaved Caspase-3 and Bcl-2 were detected by Western blotting. TheeffectsofBaohuoside-Ⅰontranscriptomeandpossiblesignaling pathways were detected by RNA-Seq technology. Results: Baohuoside-Ⅰ could inhibit the proliferation, invasion and migration of SW480 and RKO cells, and induce cell apoptosis and G0/G1 phase block. Baohuoside-Ⅰ could also up-regulate the protein expressions of cleaved PARP and cleaved Caspase-3 but down-regulate the protein expression of Bcl-2 in SW480 and RKO cell lines. In addition, RNA-Seq data analysis showed that DNAreplication and transcription of ERBB signaling pathway related genes were both affected by Baohuoside-Ⅰ. Conclusion: Baohuoside-Ⅰ could induce apoptosis and G0/G1 phase block of colon cancer cell lines SW480 and RKO by affecting the expression of apoptosis related proteins, as well as cellular DNA replication and ERBB signaling pathways, thus inhibiting the malignant phenotypes of SW480 and RKO.