Objective To evaluate the diagnose and operation about the movement cecum syndrome.Methods To analyse clinical manifestations and what finned by operation of the movement cecum syndrom 16 cases,to summarize the issues of diagnose.To use a new operation method-accending colo,cecum shorten treates the movement cecum syndrome,observing the result of the treatment.Results 1 case was used with appendectomy,2 cases was treated with appendectomy and cecum fixxing,clinical manifestation do not disappear.13 cases was treated with appendectomy and accending colo、cecum,clinical manifestation disappear,the weight added.Result of the treament is good.Conclusion appendectomy colo,cecum shorten is a good methed for treating movement cecum syndrom.

0.05). Conclusions The LTs synthesis is elevated in RSV bronchiolitis and LTs may participate in the pathogenesis of RSV bronchiolitis. Leukotriene receptor antagonist may play an important role in RSV bronchiolitis. However there still needs further research on the relationship between LTs and eosinophils count in the pathogenesis of RSV bronchiolitis.

With the development of minimally invasive surgery,great developments have been made in laparoscopie techniques and digestive endoscopy.However,laparoscopy may cause injury by abdominal puncture,and digestive endoscopy is a painful examination for patients and is difficult to localize the tumor.Magnetic anchor makes use of magnetic attraction in positioning,and it improves laparoscopy and endoscopy by external magnetic field or magnetic handle.Nowadays,combined application of magnetic anchor and laparoscope and endoscope in the treatment of digestive diseases has been accepted by many medical centers,and further researches need to be carried out to realize the aim(small trauma,little pain and quick recovery)of minimally invasive surgery.

The efficacy of conventional chemotherapy for gastric cancer is extremely limited because of its relatively low chemo-sensitivity and obvious heterogeneity. Molecular phenotype-directed target therapy is important for the improvement of the effi-cacy of conventional chemotherapy in treating advanced gastric cancer. Trastuzumab has been confirmed to have a survival benefit when added to chemotherapy for HER-2 positive gastric cancer. Ramucirumab can increase the survival of gastric cancer patients as second line treatment compared with a placebo. The anti-c-MET monoclonal antibody Rilotumumab indicates promising results in phaseⅡtrial. However, most of the targeting drugs in gastric cancer clinical trials have failed. Therefore, further efforts are required to explore critical targeted genes and effective agents.

Polyamidoamine (PAMAM) dendrimers is considered a better genetic carrier for its ability to form with DNA a nano-sized polyelectrolyte complex of high stability and dissolubility. The mechanism of PAMAMmediated gene endocytosis has been fairly clear and the researches in vitro have confirmed that PAMAM had high transfection efficiency and low cytotoxicity. The researches in vivo have further confirmed that PAMAM had a potential application foreground in the field of gene therapy including in the prevention and treatment of ewing's sarcoma and pulmonary fibrosis, in heart transplantation, and in the preparation of falciparum malaria vaccine.

Thyroid nodules are very common.Its etiological factors include hyperplasia,adenomas,cancer,cysts,and thyroiditis.Most of thyroid nodules are benign.The management of thyroid nodules whether thyroidectomy or observation is according to their nature.So,the key of the therapy is to identify that they are benign or malignant.We should comprehensive evaluate patients' history,physical examination,laboratory examination,and auxiliary examination for judging the nature of thyroid nodules and making decision in the choose of thyroidectomy or observation.

Objective To construct a yeast expression vector containing human TIP60? gene (a spliced form of HIV-1 tat interactive protein,HTATIP,60?103) and screen its interaction proteins by yeast two-hybrid. Methods Human TIP60? gene fragment was amplified by RT-PCR and cloned into pGBKT7 vector. Using TIP60? as bait,the proteins interacting with TIP60? were screened from a human liver cDNA library. The positive clones were analyzed by bioinformatic methods. Results Human TIP60? cDNA was successfully amplified and cloned into the pGBKT7 vector and indentified by double enzyme digestion and DNA sequencing. Using yeast two-hybrid,32 clones were screened from a human liver cDNA library,and 9 positive clones including histone deacetylase 7A (HDAC7A),mouse double minute 2 (DMD2),B-cell CLL/lymphoma 3 (BCL3),endothelin receptor type A (EDNRA),androgen receptor (AR),PHD finger protein 17 (PHF17),Ataxia Telangiectasia Mutated (ATM),CAMP responsive element binding protein 1 (CREB1),orphan steroid receptor BD73 were verified after sequencing. Conclusion The screened interaction proteins with TIP60? by yeast two-hybrid may be involved in the roles of transcriptional regulation of TIP60?.

Objective:To research the expense distribution of discharged patients during hospitalization to find out the period with the greatest weight, and provide reliable evidence for the increase of turnover rate by controlling hospitalization of patients and medical security. Methods:Totally 2 444 discharged patients of our hospital from September to November in 2013 with stay day of 1 to 20 d were analyzed, and then divided into the internal medicine group and surgical groups including surgical operation group and surgical without operation group. The hospitalization expenses and drug expense in all the groups were counted and compared. Results: The daily expense in the internal medicine group showed significant difference when compared with that in the surgical operation group and surgical without operation group (P<0. 01), meanwhile, that in the two surgical groups had no significant difference (P>0. 05). The daily expense in the surgical operation group was with linear decrease, and that in the other groups was with logarithmic decline. The 10-day expense in each group had significant difference (P<0. 05) compared by median test. The expense proportion of drugs in all groups was generally increased along with the hospitalization days. Conclusion:The effective method for the decrease of drug pro-portion is shortening hospitalization days,especially in surgical departmens,the drug proportion is increased along with the length of stay.

AIM: To detect the expression of ALEX 1 in the breast cancer tissues in order to verify whether ALEX1 has correlation with clinical pathological features in breast cancer .METHODS:Real-time PCR and immmunohis-tochemistry were applied to detect the expression of ALEX 1 at mRNA and protein levels in the breast tissues .The statistical analysis were performed for determining the correlation with the level of ALEX 1 and the clinical pathological features in breast cancer .RESULTS:The protein levels of ALEX1 in the breast cancer tissues were lower than that in the non-breast cancer tissues (P<0.01).The expression of ALEX1 had correlations with pathological grade , clinical stage, molecular type (P<0.05) but had no correlation with the patients ’ age, tumor size and tumor types in breast cancer .Furthermore, the result of real-time PCR showed that mRNA expression of ALEX 1 was also significantly reduced in the breast cancer tis-sues (P<0.01).CONCLUSION:The expression of ALEX1 in the breast cancer tissues is lower than that in non-breast cancer tissues .The pathological grade and clinical stage in breast cancer are negatively correlated with the expression of ALEX1.

Objective To observe the clinical curative effect of the joint application of Vitapex paste and AH plus,which cured the combined periodontic -endodontic lesion .Methods 72 patients of 113 teeth with the com-bined periodontic-endodontic lesion were chosen ,which were randomly divided into the two groups ,with all complete periodontal initial therapy and routine root canal preparation and dressing at the same time .The observation group included 36 cases of 58 teeth,when the first time to see a doctor ,injected Vitapex paste to the periapical tissue and periodontal tissue sparse area for 1 -2weeks.The sceond time,the tap root canal was closely filled withc AH plus paste and gutta-percha.The control group concludes 36 cases of 55 teeth,with the traditional method,the tap root canal was filled with AH plus paste and gutta -percha directly .Then observation of postoperative emergency response within 1 week,of periodontal clinical indicators (including the PD,BOP,PLI) in 1 month,3 months,6 months after periodontal initial therapy ,and of the long-term curative effect after 1 year.Results 1 week after root canal filling , there were 48 cases no reaction , 9 cases mild reaction and 1 case severe reaction in the observation group , while 47cases no reaction ,7 cases mild reaction and 1 case severe reaction in the control group .There was no statistical difference of evaluation of emergency response between the two groups (χ2 =0.002,P>0.05).Compared with the situations before treatment ,PD,BOP and PLI were all reduced significantly in 1,3 and 6 months after periodontal ther-apy (P<0.05).There was no significant differences of PD ,BOP and PLI between the two groups before treatment (t=0.050,0.019,0.004,all P>0.05),while 1 month (t=2.581,5.984,1.870,all P<0.05),3 months (t=4.806,7.640,1.034,all P<0.05) and 6 months (t=4.612,8.043,1.167,all P<0.05) later,there was statistical difference of decreased value between the two groups .The 1 year follow up showed that significant efficiency in the observation group was 46.55%,and total efficiency was 91.38%,while significant efficiency 27.27% and total effi-ciency 70.91%in the control group ,and there were statistical differences of significant efficiency and total efficiency between the two groups (χ2 =16.761,21.061,all P<0.05).Conclusion With the joint application of Vitapex paste and AH plus ,and two method curing the combined periodontic -endodontic lesion ,there was no evident differ-ence compared with the traditional short -term emergency response .By long-term observation ,found that this method could effectively promote the healing of periapical tissue and periodontal tissue ,and that curative effect is remarkable compared with the traditional method .