Objective To develop a kind of tongue image analysis instrument which is used to deal with the deviation problem on the process of tongue image analysis currently. Methods The system included two parts:hardware and software. The hardware was made up of special tongue image acquisition device and computer. With the application of digital image analysis technology, the instrument implements functions such as tongue image collection, image preprocess, image segmentation and feature analysis. Results After a certain amount of sample study process, the accuracy of tongue shape recognition was about 90%. Conclusion This instrument can achieve the aim of tongue information objectification and are widely used for clinical medicine and teaching.

Objective To understand the situation of nosocomial infections, the distribution of important pathogens of affected patients and the uses of antibacterial drugs.Methods The cross-sectional survey was ap-plied.Before the survey, the investigators were trained by the professionals of the department of hospital infection management.Medical records were reviewed one by one, checked with bedside physical examination.Results In this survey, 283 inpatients were investigated, with an actual check rate of 100%.Two inpatients were affected with nosocomial infection with the prevalence rate of 0.71%.There were two cases of infections (0.71%).Conclusion Investigation of the prevalence rate of nosocomial infection was an important means to monitor the hospital infection, which can monitor specifically, minimize the incidence of nosocomial infection and ensure medical safety by obtaining the information of the departments with high-risk nosocomial infection, the main infectious sites and pathogens.

The culture of umbilical cord-derived mesenchymal stem cells is extremely important for studies on umbilical cord mesenchymal stem cells. Optimization of cellculture technology is crucial for clinical application of mesenchymal stem cells and even celltherapy. Meanwhile, the labeling and tracer technique of umbilical cord-derived mesenchymal stem cells is a hotspot in stem celltransplantation. OBJECTIVE:To review the research and development of the cellmarkers and tracer methods of umbilical cord-derived mesenchymal stem cells. METHODS:A computer-based search of VIP, CNKI, Medline, Highwire and Foreign Journals Integration System databases was performed for articles concerning culture and labeling of umbilical cord-derived mesenchymal stem cells published from January 2001 to October 2013. The keywords were“stem cells, mesenchymal stem cells, umbilical cord-derived mesenchymalstem cells, cellculture, labeling methods”in Chinese and English, respectively. Final y, 35 articles were included in result analysis. RESULTS AND CONCLUSION:Umbilical cord-derived mesenchymal stem cells have not yet been widely used, mainly because of the immature isolation, culture and staining techniques of umbilical cord-derived mesenchymal stem cells. These techniques are worthy of further optimization studies. Although in recent years, cellmarkers and tracer technology of umbilical cord-derived mesenchymal stem cell s have made great progress, there are stil many problems need to be solved.

AIM: To investigate the expression profile of peripheral-type benzodiazepine receptor(PBR) involved in mitochondrial permeability transition(PT) regulation,and to observe the binding dynamic of the mitochondrial PBR with specificity ligand during rat live regeneration.METHODS: Liver regeneration model was produced by 70% partial hepatectomy(PH) performed in male SD rats.The animals of sham groups underwent the same surgical operations as PH groups did,but the liver lobes were not resected.The animals in the PH groups and corresponding sham groups were sacrificed at 3,6,12,24,48,72,120 and 168 hours after the operation.The livers were removed,weighted and processed for isolation of mitochondria.Semi-quantitative RT-PCR was performed to examine the expression level of PBR in 70% hepatectomized rat livers during the whole regeneration process and compared to that in the sham and normal groups.Compared with healthy rats,the kinetic parameters of PBR was evaluated by using a specific radioligand -PK11195.RESULTS: Compared with healthy rats,the expression of PBR was unchanged.Meanwhile,the results obtained in the present experiments by scatchard analysis,Bmax of PK11195 for PBR significantly decreased,returned to normal level in 168 h after PH.Kd of PK11195 for PBR significantly decreased at 72 h and 168 h after PH of rat liver regeneration(P

Objective To investigate the saponins from the Chinese materia medica Huluba (the seeds of Trigonella foenum-graecum L.). Methods To isolate and purify the single saponin by the column chromatography and dry column chromatography of silica gel H. The structural elucidation was carried on by spectroscopic evidence of (()~(13)C-NMR), FAB-MS, DEPT and the results of the fraction-hydrolysis of acquiring the secondary glucosides. Results The newly isolated saponin D was consisted by six molecules of sugar with diosgenin. The chemical structure of saponin D is: diosgenin-3-O-?-L-rhamnopyranosyl (1→3)-?-D-glucopyranosyl (1→4)-?-L-rhamnopyranosyl [(1→3)-?-L-rhamnopyranosyl] (1→4)-?-D-glucopyranosyl (1→4)-?-D-glucopyranoside. Conclusion Saponin D is a new one consisted of six molecules of su-(gar) with diosgenin.

Object To investigate the saponin from the seeds of Trigonella foenum graecum Linn (STFG) Methods The total saponin from STFG was extracted and purified by using the absorptive resin, the single saponin was isolated by using the column chromatography as well as dry column chromatography of silica gel H The chemical structure was elucidated by 13 CNMR , FAB MS, DEPT spectroscopic evidence and the results of fraction hydrolysis of acquiring their secondary glucosides Results A new saponin A from the total saponin has been obtained, the fraction hydrolysis carried out and the secondary glucoside Ⅰ and Ⅱ identified by determining the structure of saponin A The chemical structure of saponin A is: diosgenin 3 O ? L rhamnopyranosyl(1→4) ? D glucopyranosyl(1→4) ? D glucopyranoside The secondary glucoside Ⅰ is: diosgenin 3 O ? D glucopyranoside; Ⅱ is: diosgenin 3 O ? D glucopyranosyl(1→4) ? D glucopyranoside Conclusion Glucoside A is a new saponin with three molecules of sugar

Object To do detail investigation of the saponins from the Chinese materia medica Huluba (the seeds of Trigonella foenum graecum L ) Methods The pure saponins from the total saponins were isolated by employing the column chromatography and dry column chromatography of silica gel H Their chemical structures were elucidated by 13 C NMR , FAB MS, DEPT spectroscopic evidence and the results of the fraction hydrolysis of acquiring their secondary glucosides were obtained Results Two new saponins B and C were isolated and both were the glucosides consisted by four molecules of sugar with diosgenin The chemical structure of B is: diosgenin 3 O ? L rhamnopyranosyl (1→3) ? L rhamnopyranosyl (1→4) ? D glucopyranosyl (1→4) ? D glucopyranoside And saponin C is: diosgenin 3 O ? D glucopyranosyl (1→4) ? L rhamnopyranosyl (1→4) ? D glucopyranosyl (1→4) ? D glucopyranoside Conclusion Saponins B and C are two new ones with four molecules of sugar respectively

Objective: To investigate the development of public health human resource of the Centers for Disease Control and Prevention (CDCs) in Zhejiang Province from 2016 to 2020, so as to put forward the strategies for the talent team construction of CDCs. Methods: The general information of personnel of CDCs in Zhejiang Province from 2016 to 2020 was collected through China Information System for Disease Control and Prevention. A questionnaire survey and a qualitative interview were conducted to collect the data of staffing, talent training, salary and employee turnover, and to analyze the development of the talent team of CDCs. Results: By 2020, there were 105 CDCs, including 1 provincial, 11 prefectural and 93 county-level CDCs, and 5 277 staff on-the-job. From 2016 to 2020, the number of staff per 10 000 permanent residents in the province, prefecture and county levels were 0.062-0.070, 0.170-0.188 and 0.585-0.604, respectively, which did not meet the standard for the personnel establishment in Zhejiang CDCs; the proportion of the staff with bachelor degree or above increased from 90.05% to 94.25%; the proportion of the health professionals decreased from 82.91% to 80.01%; the proportion of the staff with senior professional titles increased from 35.20% to 45.25%. The results of the qualitative interview indicated a lack of health professionals and high-end talents, a gap between the approved staffing and post requirements, as well as demands for a stable team and higher pay. Conclusion From 2016 to 2020, the structure of educational background, major and professional title among the on-the-job staff of CDCs in Zhejiang Province has been improving; however, the total allocation of human resource is insufficient, and the stability and treatment of staff need to be further improved.

Objective To observe the clinical effect of electric acupuncture,surrounding acupuncture and cotton moxibustion in the treatment of herpes zoster.Methods Forty cases with herpes zoster were randomly divided into a treatment group and a control group,with 20 patients in each group.The treatment group was treated with electric acupuncture,surrounding acupuncture and cotton moxibustion,while the control group was treated with valaciclovir and vitamin B1.In the two groups severity of pain and the time of pain beginning to ease,pain lasting blisters disappearing,incrustation and decrustation during treatment were observed and recorded,referring to the People's Republic of China Traditional Chinese Medicine Industry Standard(Chinese disease diagnosis and efficacy standard)for efficacy evaluation.Results There was significant difference between the treatment group and the control group with regard to the cure rate(75% versus 35%,P＜0.05);however,the total effective rates of two groups had no significant difference(P＞0.05).After treatment VAS score in treatment group was significantly lower than that in control group(P＜0.05);in treatment group the time of pain beginning to ease and pain lasting were significantly less than those in control group(P＜0.05);the time of blister disappearing in treatment group was significantly faster than that in control group(P＜0.01);the time of incrustation and decrustation in treatment group were significantly less than those in control group(P＜0.05).Conclusion The advantages of electric acupuncture,surrounding acupuncture and cotton moxibustion in the treatment of herpes zoster were shown as faster pain easing,shorter pain lasting,shorter duration of blister and incrustation.This treatment for herpes zoster is safe,effective and affordable.

In subjects aged above 40 years old in Pudong,Shanghai,the annual progression rates from normal glucose regulation to impaired glucose tolerance and to diabetes were 9.5%and 4.4%respectively.and the annual progression rate in subjects with impaired Slucose regulation to diabetes was 20.2%.The conversion rate to diabetes increased along with elevated number of risk factors.