Objective To discuss the perioperative nursing method for patients receiving colorec-tal tumor surgery under peritoneoscope. Methods Using the nursing procedure, through appraisal of patients' health situation→listing the nursing diagnosis or the nursing questions→formulation of nursing plan→ implementation of nursing plan→appraisal of the expected effect, combination of the surgical nursing's characteristic, to practice the idea of "the perfect person health and concern", cooperation with medical and nursing team, we provided a complete treatment and nursing plan for 38 patients receiving colorectal tumor surgery under peritoneoscope. Results 38 patients made satisfactory results with no surgery complications. The average in-hospital time was 9 days. Conclusions This technology has slight wound,light hemorrhage,slight draw-off, less pain, short exposure time of intestine, fast recovery of intestine function and early food intake. Systematic and intact perioperative nursing can improve the nursing quality and the success rate and reduce the complications so that the patients can live through the surgery safely.

Objective To explore the clinical behavior,pathological and immunohistochemical characteristics and diagnosis, treatment methods of gastrointestinal stromal sarcomas (GISS). Methods Retrospective analysis was perfomed over 36 GISS patients based on clinical data and pathological data. Results GISS occurred more in the stomach and small intestine, than colon and rectum. Immunohistochemical staining showed CD117, CD34 expression,the positive rates were 91.7% and 86. 1%. Conclusion GISS is a rare malignant tumor, GISS diagnosis depends on the histologic and immunohistochemical test. CD34 and CD117 in the positive diagnosis has its special significance. Surgery is the main treatment.

Bronchopulmonary dysplasia is one of the causes of affecting children′s health,which has a complicated etiology,including the gene level of internal aspect and some external factors, such as premature birth inducing the pulmonary hyperplasia,infectious diseases caused by low immunity,complications caused by clinical interventions,and the low quality of respiratory tract management. In the article,we review the research progress about mechanism and prevention of BPD in domestic and overseas in recent years.

Mechanical stresses modulate almost all aspects of cell function, including growth, differentiation, migration, gene expression, protein synthesis and apoptosis. Thus, uncovering the mechanisms by which living cells sense and transduce mechanical stress lies at the core of understanding how they respond and adapt to their physical environments. In this review, the possible mechanism about cell response to mechanical stimulus was discussed from outer cell membrane to inner nucleus.

Objective To observe the clinical effect of procedure for prolaps and hemofrhoids (PPH) in treatment of mixed hemorrhoids combined with non-functional obstructed defecation syndromes (ODS).Methods The clinical data of 50 patients with mixed hemorrhoids combined with non-functional ODS treated by PPH were retrospectively analysed.All these patients were followed up for 12 months,the treatment outcome was observed.Results Eleven cases were basic cured,apparently effective in 12 cases,and effective in 12 cases,invalid in 15 cases,the total effective rate was 70％ (35/50),inefficacy patients were mainly combined with rectocele.None patients had serious complications such as death,anastomotic infection and pelvic sepsis.Conclusion PPH convenient operation,small trauma,for the treatment of mixed hemorrhoids combined with internal rectal prolapse is safe and effective,but the effective is poorer in treatment of mixed hemorrhoids combined with rectocele.

Objective To investigate the curative effects of laparocopic treatment for cystiduodenal internal fistula. Methods All procedures were conducted under laparoscope. After the exposure of the cholecystic base, trans-cholecystic cholangiography was performed to detect the relations between the cholecyst and neighboring organs. Once the internal fistula was confirmed, the orifice of fistula was closed laparoscopically. Then abdominal drainage, gastrointestinal decompression, and administration of somatostatin were routinely employed. Results In a series of 327 cases of laparoscopic cholecystectomy (LC) with intraoperative cholangiography, a total of 6 cases of cystiduodenal internal fistula were detected, all of which were anatomized and closed successfully under laparoscope. No postoperative hemorrhage or duodenal bile leakage occurred. Conclusions Routine intraoperative cholangiography during LC is beneficial to the detection of the cystiduodenal internal fistula.Effective and reliable management rests upon skillful laparoscopic techniques of dissection and fistula closure, as well as the supportive treatment.

Objective: To establish an on-line column switching liquid chromatography for quantitative determination of vitamin D3 in poultry eggs and their products. Methods: Antioxidant-protected samples were saponified, extracted with a mixture of ethyl acetate-n-hexane mixture (6︰4, v/v) and subjected for determination using two-dimensional liquid chromatography. One-dimensional chromatography was performed with the UniChiral® OD-5H column (4.6 mm × 100 mm, 5 μm), acetonitrile: methanol (75︰25, v/v) and water as a mobile phase for gradient elution at a flow rate of 1.00 mL/min to complete vitamin D purification, and two-dimensional chromatography was performed with the Agilent Eclipse PAH column (2.1 mm × 100 mm, 3.5 μm, column temperature of 35 ℃), detection wavelength of 264 nm for separation and determination of vitamin D3 and internal standard vitamin D2 with the internal standard method. Results: Vitamin D3 had a good linear relationship within the range of 2.5 to 100 ng/mL, with r2 of >0.999. The mean spiked recovery rate of vitamin D3 was 101.23% to 102.08% in egg yolk powder, with relative standard deviation (RSD) of 3.91% to 5.85%, detection limit of 1.5 μg/100 g, and quantitative limit of 4.9 μg/100 g, and the limits of detection and quantitation of vitamin D3 were 0.15 μg/100 g and 0.49 μg/100 g in whole egg liquids. Conclusions The method is simple, rapid, highly accurate, sensitive and reproducible, which is suitable for rapid and quantitative determination of vitamin D3 in poultry eggs and their products.

Objective To investigate the effects of resveratrol on epileptic seizure and cognitive impairment and the role of CREB in rats with temporal lobe epilepsy (TLE). Methods TLE rat model was established by LiCl?pilocarpine injection and resveratrol was administrated at a dose of 15 mg/(kg · d) for 7 days. The rat behavior was evaluated by Racine scale and the CREB expression and phosphorylation in temporal cortex were detected with western blot. The Morris water maze was used to assess the learning and memory. Results Resveratrol administra?tion significantly reduced the Racine scales of TLE rats. The CREB phosphorylation in TLE group was increased compared with that in control group, which was remarkably inhibited by resveratrol. The escape latency of TLE group was increased at 4th, 5th and 6th day after training, and time in target quadrant was reduced compared with that in control group. Resveratrol significantly decreased latency time and increased target quadrant time of TLE rats. Conclusion In TLE rats, resveratrol administration suppresses epileptic seizure and improves cognitive function, which might be mediated by inhibition of CREB phosphorylation.

Objective To explore expression of Beclin1, autophagy-related protein 1 (Atg1), mammalian target of ra-pamycin (mTOR) in squamous cell carcinoma (SCC) and adenocarcinoma of lung and to analyse relationship among these three factors. Methods Immunohistochemical stainning was applied to detect expression of Beclin1, Atg1 and mTOR in 82 samples of SCC and adenocarcinoma of lung (lung cancer group) and 17 samples of paraneoplastic normal lung tissues (con-trol group). Correlations of expression of Beclin1, Atg1 and mTOR were analyzed in SCC and adenocarcinoma of lung. Re-sults The expression of Beclin1 was significantly lower in lung cancer group than that in control group(52.44%vs 94.12%, P＜0.01). There were significant differences of the differentiation, TNM stages and lymph node metastasis in lung cancer group compared with those in control group. And Beclin1 expression showed a decreasing trend with malignant and invasive of tumors. mTOR expression in lung cancer tissue with lymph nod metastasis is higher than that in the tissue without lymph node metastasis(P＜0.01). Atg1 expression in lung cancer tissue with larger mass is less than that in lung cancer tissue with small lumps(P＜0.01). Beclin1 Expression was positively correlated with the Atg1 expression in SCC(r=0.609,P＜0.01). Beclin1 and Atg1 expressions were both negatively correlated with mTOR expression in SCC(r=-0.617,-0.444,P＜0.01). Beclin1 expression was positively correlated with Atg1 expression in adenocarcinoma(r=0.458,P＜0.01). Beclin1 and Atg1 expression were both negatively correlated with mTOR expression in adenocarcinoma(r=-0.497,-0.541,P＜0.01). Conclu-sion Beclin1, Atg1 and mTOR expression showed a strong correlation in lung cancer, and clinicopathological change of lung SCC and adenocarcinoma may be related to autophagy.

Purpose To study the correlation between the expression of podoplanin-positive stromal cells and clinicopathologic features in squamous cell carcinoma ( SCC) of esophagus, and to explore the role of podoplanin-stromal cells in the progression of SCC of e-sophagus. Methods Immunohistochemical EnVision method was used to detect the expression of podoplanin in the stromal cells of e-sophageal SCC. The correlation between the expression of podoplanin-positive stromal cells and clinicopathologic features in SCC of e-sophagus was analyzed. Results Of 69 cases of SCC, podoplanin-positive stromal cells were seen in 43 and the positive rate was 62. 3%. Lymphatic vessel density in SCCs with podoplanin-positive stromal cell was 45. 72 ± 14. 36, which was higher than that with-out podoplanin-positive stromal cell (21. 23 ± 10. 49) (P<0. 01). Expression of podoplanin in stromal cells in SCCs with lymphatic vessel invasion was higher than those without lymphatic vessel invasion (P<0. 05). There was no correlation between podoplanin-posi-tive stromal cells and age, sex, tumor size, the depth of invasion, differentiation, lymph node metastasis, and clinical stage ( P>0. 05). Conclusion Podoplanin-positive stromal cells may promote the lymphangiogenesis and lymph vessel invasion of esophageal SCC.