*Alkaloids ; *Antineoplastic Agents, Phytogenic ; Chemistry ; *Harringtonines

Objective To analyze the influence of anticoagulants on platelet count .Methods A total of 5 patients with decreased platelet level ,detected by using haemocyte analyzer and samples anticoagulated by ethylene diamine tetraacetic acid dipotassium (EDTA‐K2 ) ,were enrolled ,and EDTA‐K2 ,sodium citrate ,heparin lithium ,sodium fluoride anticoagulated venous blood samples and fingers peripheral blood samples were collected and detected for platelet by using haemocyte analyzer and microscopic detection . Wright‐Giemsa′s stained cell smears were prepared and observed by using microscope .Results Platelet levels of anticoagulated samples ,detected by haemocyte analyzer and microscopic detection were obviously decreased ,and with large platelet aggregation ob‐served under microscope .Platelet levels of finger peripheral blood samples detected by haemocyte analyzer and microscopic detection were normal ,and without platelet aggregation observed under microscope .Conclusion Various anticoagulants could cause pseudo‐thrombocytopenia ,and fingers peripheral blood samples ,without anticoagulants ,should be used for platelet count to obtain accurate results .

OBJECTIVE:To probe into the utilization status of carbopenem antibacterials in our hospital.METHODS:The antimicrobial use density(AUD),the antimicrobial average daily cost(AADC),and the antimicrobial cost density(ACD) of carbopenem antibacterials used in our hospital during 2000～2006 were analyzed statistically using DDDs analytical method. RESULTS:DDDs and AUD of the carbopenem antibacterials used in our hospital showed an upward trend;the AADC of each kind was decreasing year by year but ACD presented an upward trend.CONCLUSION:Safe,effective,economical and rational use of carbopenem antibacterials is in the interest of protection of drug resources used for bacterial infection.

Objective:To study the clinical features of histiocytic necrotizing lymphadenitis. Method: Eleven pa-tients with histiocytic necrotizing lymphadenitis were collected to analyze the clinical features, pathological features of biopsy lymph nodes,diagnosis and treatment. Result:Lymph node swelling of neck and persistent fever were de-tected in all patients. The leukocyte reduction and swiftness of ESR in serum were detected in most patients. The final diagnosis of histiocytic necrotizing lymphadenitis was confirmed by biopsy investigation of the pathology. The pathological features included distinctive necrosis, loss of lymph node structure, infiltration with histiocytes and lymphocytes, absence of neutrophils. All patients were treated with glucocorticoid for 2 to 4 months. The patients improved significantly and were not recurrence during follow up visit. Conclusion: There was no specific clinical manifestation of histiocytic necrotizing lymphadenitis and which easy to misdiagnose. Diagnosis of histiocytic ne-crotizing lymphadenitis relies on the pathological examination of enlarged lymph nodes.

Objective To investigate the therapeutic effects of retinal angiomatosis in differentclinical stages.To discuss the indication of vitrectomy for retinal hemangioblastoma. Methods TheclinicaI data of 22 cases(33 eyes)were retrospectively analyzed.The retinal hemangiomas were dividedinto 5 stages according to their degrees of development from simple angioma without vessel dilation tofeeder vessel dilation and intra-retinal exudates.local retinal detachment,massive retinal detachment andcomplication occurrence in proper order.The methods of treatment were laser photocoagulation,trans-scleral cryotherapy and vitrectomy. 13 eyes were treated with laser photocoagulation, 5 eyes withcryotherapy combined with laser and 11 eyes with vitrectomy.Tumor resection and silicone oil tamponadewas performed in 3 eyes during vitrectomy.The patients were followed up for 46 months on average.Visual acuity(VA),the condition of the hemangioma and retina was compared pre～and post-operationrespectively. Results In a11 13 eyes treated with laser phot.coagulation the hemangiomas regressed andthe retina remained attached.VA improved in 2 eyes,and remained unchanged in 11 eyes.Cryotherapycombined with laser photocoagulation was performed on 5 eyes. In this group,4 eyes'hemangiomasregressed and no new hemangiomas occurred,proliferative vitreous retinopathy and vitreous hemorrhagewas observed in 1 eye which vitrectomy was performed later.VA improved in 2 eyes,unchanged in 2 eyesand decreased in 1 eye.In the 11 eyes treated with vitreoretinal surgery,new hemangiomas was found in 1eye,exudative retinal detachment was caused by hemangiomas in 2 eyes,proliferative vitreous retinopathywas observed in 2 eyes.and the retina remained attached in 8 eyes.VA improved in 3 eyes,unimproved in3 eyes,and decreased in 5 eyes.In the 3 eyes with surgical resection of retinal hemangioma during vitrectomy,2 eyes'retina remained attached,1 eye had exudative retinal detachment and no new hemangiomas occurred.VA improved in 2 eyes and decreased in 1 eye. Conclusions Laser photocoagulation or combined with cryotherapy is effective in treating the hemangiomas in early stage.Vitreetomy is advisable for late stage of retinal angiomatosis,especially with vitreous hemorrhage,epiretinal membrane,proliferation and large scale of retinal detachment.Surgical resection of isolated large retinal hemangioblastoma may be useful for selected patients.

Objective To investigate the efficiency of intracerebroventricular injection of histamine receptor antagonists on mechanical pain threshold of neuropathic pain in rats. Methods The neuropathic pain rat model was established by partially ligation of the sciatic nerve in 30 rats. Two weeks later, histamine receptor 1 antagonist pyrilamine 1.5 μg or 15 μg was injected intracerebroventricularly in group P1 or P2, histamine receptor 2 antagonist ranitidine 10 μg or 100 μg in group R1 or R2, and normal saline in group C as the control. The pain threshold was measured. Results Compaered with group C,the pain threshold was signifigantly increased in group R1 and R2, especially in group R2 (P<0.05). Conclusion Histamine H2 receptor antagonist can increase pain threshold in rats with neuropathic pain.

Objective To study the therapeutical effects of compound Danshen injection combined with edaravone in treatment of acute cerebral infarction.Methods 130 cases of ACI were randomly divided into two groups, control group(65 case) and observation group(65 cases), control group were administered with edaravone injection, while observation group were administered with compound Danshen injection and edaravone injection, NIHSS score and Barthel index score were used for effects evaluation, meanwhile, serous levels of copeptin, NT-proBNP and Hcy were measured by ELISA assay.Results Observation group had a better effect on ACI care, compared with control group (P<0.01), and serum levels of copeptin,NT-proBNP and Hcy significantly improved both in the two groups ( P <0.01 ) , while, observation group had a better effects ( P <0.01 ) . Conclusion Combination therapy of compound Danshen injection and edaravone has a fabulous effect on ACI care.

Objective To study the clinical efficacy of modified Liuwei Dihuang Decoction (LDD) combined with metformin plus atorvastatin for the treatment of type 2 diabetes mellitus (T2DM) complicated with hyperlipidemia. Methods A total of 120 cases of T2DM patients complicated with hyperlipidemia were randomly divided into study group (N=60) and control group (N=60), which were treated with LDD combined with metformin plus atorvastatin orally, and with oral use of metformin plus atorvastatin respectively. The course of treatment lasted for 12 weeks. Before and after treatment, we compared the blood levels of fasting blood glucose (FBG), 2-hour postprandial blood glucose(2hPG), glycosylated hemoglobin(HbA1C), total cholesterol(TC), triglyceride(TG), high-density lipoprotein cholesterol(HDL-C), and low-density lipoprotein cholesterol(LDL-C) in the two groups. After treatment, we evaluated the therapeutic effect of the two groups. Results (1) The total effective rate of the study group was 95.0％, and that of the control group was 83.3％, the effective rate of the study group being significantly higher than that of the control group (P < 0.05). (2) After treatment, the blood levels of FBG, 2hPG and HbA1C of both groups were significantly lower than pre-treatment levels (P <0.001), and the levels of FBG, 2hPG and HbA1C of the study group were significantly lower than those of the control group (P<0.05, P<0.01 or P<0.001). (3) After treatment, the TC, TG and LDL-C levels of both groups were significantly lower and HDL-C was higher than pre-treatment levels (P < 0.001); the levels of TC and TG levels of the study group were significantly lower and HDL-C was higher than those of the control group (P < 0.05 or P < 0.001). Conclusion LDD combined with metformin plus atorvastatin exerts certain effect for the treatment of T2DM complicated with hyperlipidemia, and is more effective than metformin or atorvastatin alone.

OBJECTIVE:To explore the observation and nursing points of hemorrhage in intravenous thrombolysis after using alteplase. METHODS:128 patients underwent alteplase intravenous thrombolysis were selected as the research object. The occur-rence of hemorrhage in each part were observed within 48 hours after medication and nursed timely. RESULTS:128 patients re-ceived intravenous thrombolysis. 48 h later,26 patients suffered from hemorrhage complications (20.3%). Among them,there were 6 cases of nose bleeding (4.7%),8 cases of subcutaneous congestion (6.3%),6 cases of gingival bleeding (4.7%) and 6 cases of gastrointestinal bleeding(4.7%). There was no significant difference in the incidence of complications among those groups (P>0.05). The time of nose bleeding was (3.2 ± 1.3) h,the time of subcutaneous congestion (3.5 ± 1.2)h,the time of gingival bleeding(1.5±0.7)h,and the time of gastrointestinal bleeding(4.1±1.8)h. The time of gingival bleeding was earlier than that of other groups,with statistically significant difference(P<0.05). There was no statistically significant difference among other groups (P>0.05). CONCLUSIONS:In alteplase intravenous thrombolysis,nurses should closely monitor hemorrhage and complications, especially the complication of gingival bleeding. Find out the complications timely and nurse it as early as possible.

Objective To investigate the clinical value of shortening double-J stent indwelling time in the treatment of ureteral stone-street complications after flexible ureteroscope lithotripsy . Methods Clinical data of 38 cases of ureteral stone-street complications following flexible ureteroscope lithotripsy from January 2012 to December 2013 in our hospital were retrospectively analyzed.The patients were divided into Group A (extubating the double-J stent two weeks after the surgery ) and Group B (retaining the double-J stent) with 19 cases in each group .The stone clearance results were compared . Results All the urinary calculus in Group A had been removed successfully , including 2 cases of renal colic , 3 cases of irritative symptoms of bladder and 3 cases of gross hematuria.Hospital cost was (766.5 ±153.7) yuan.Urinary calculus were successfully removed in 13 cases in Group B, including 19 cases of gross hematuria and 17 cases of irritative symptoms of bladder.No renal colic occurred.The hospital cost was (1251.2 ± 155.6) yuan.Compared with Group B , Group A has higher success rate of stone clearance , lower hospital cost and lower rate of bladder irritative symptoms and gross hematuria (P<0.05).There were no significant differences between the two groups in the occurrence rate of renal colic (P>0.05). Conclusions Removing the double-J stent 2 weeks after flexible ureteroscope lithotripsy resulted in higher stone clearance rate and less complications compared with retaining the double -J stent.It can reduce the occurrence of irritative symptoms bladder and gross hematuria .