1.GENETIC POLYMORPHISM OF SIX Y CHROMOSOMAL STR IN CHINESE HUI ETHNIC GROUP
Bofeng ZHU ; Guiping Lü ; Guifa YAO ; Jun ZHU ; Hongwang DONG ; Qingdong SUN ; Lei HUANG ; Yao LIU
Journal of Pharmaceutical Analysis 2005;17(1):49-52
Objective To study genetic polymorphism of 6 Y chromosomal STR in Hui ethnic group living in Ningxia Hui ethnic autonomous region, in order to evaluate their usefulness in forensic science and enrich the Chinese genetic information resources. Methods We investigated 101 unrelated, healthy, male individuals of Hui ethnic group and studied their allelic frequency distribution and haplotype diversity of 6 Y chromosomal STR. Primer for each loci was labeled with the fluorescent by FAM (blue) or TAMRA(yellow). The data of Hui ethnic group were generated co-amplification, GeneScan, genotype, and genetic distribution analysis. Results 31 alleles and 43 phenotype(DYS385) were detected, with the frequencies ranging from 0.0099-0.7129. Out of a total of 101 individuals, 96 showed different haplotypes; 91 were unique; 5 were found 2 times. The haplotype diversity for 6 Y-STR loci was 0.9990. Conclusion The date obtained can be valuable for individual identification, paternity testing in forensic fields and for population genetics because of 6 Y-STR loci high polymorphism.
2.An Approch on Using Concentration of Air Negative Ion and Oxygen Consumption as Environmental Monitoring Indexe for Evaluation of Indoor
Wenxing PU ; Guifa JIANG ; Mingli HUANG ; Hao LIANG ; Yuqiao QIN ; Jianjun YU ; Xuefeng LIU ; Wei Li ; Ming XU ; Huining
Journal of Environment and Health 1989;0(06):-
In this paper,the indoor air quality of several hotelsand dancing halls was evaluated by using of two monitoring indexes.The results showed that the concentration of carbon dioxide of three dancing halls is low before opening,and the oxygen consumption is low too.But after opening an hour,the concentrations of carbon dioxide and carbon monoxide raises with increase of oxygen consumption correlation analysis between the concentration of air-negative ion and the oxygen consumption showed that both is negative correlation,(r=-0.354,P
3.Research on Relationship Between Processing Time, Chroma Value and Fingerprint of Dipsaci Radix
ZHANG Xuelan ; LUO Yu ; ZHONG Zhikui ; LIN Weixiong ; HUANG Guifa ; HU Yi ; LIANG Zhiyi ; LIU Xiaotong
Chinese Journal of Modern Applied Pharmacy 2023;40(16):2231-2236
OBJECTIVE To study the correlation between processing time, chroma value and UPLC fingerprint map of Dipsaci Radix. METHODS Established the UPLC fingerprint of Dipsaci Radix. Monitored the changes of chemical components in the processing process of wine and salt processed Dipsaci Radix, spectrophotometer was used to objectively quantify the chroma value of different processed products. SPSS 20.0 and SIMCA 14.0 statistical software was used to analyze the correlation between processing time and chroma value and fingerprint. RESULTS In the process of processing, the decoction pieces color of the powder deepened and L*, b* and E* values decreased. The correlation analysis showed that the processing time was significantly correlated with the chroma value and fingerprint of decoction pieces. CONCLUSION The method of UPLC fingerprint is stabled and reliabled, combines with the objective discriminant analysis of the chroma value of the Dipsaci Radix processed products, which lay the foundation for standardizing the processing technology of wine and salt processed products and evaluating the quality of Dipsaci Radix.