Long noncoding RNA ( lncRNA) is a functional RNA molecule longer than 200 nucleotides.They are involved in epigenetic, transcriptional and post-transcriptional modifications, playing an important role in gene expres-sion, chromatin remodeling, histone modification, X chromosome inactivation, genomic imprinting, protein folding and ac-tivity as well as other important biological events.Some studies have proven that lncRNAs are involved in the development of metabolic diseases.The changes of their expression levels may be new diagnostic markers and therapeutic targets.

Objective To investigate the effect of CD147 monoclonal antibody (mAb) on the natural resistance to paclitaxel (TAX) in the human cervical cancer line (HCE1) multicellular spheroid (HCE1/MCS) model and if CD147 mAb can reverse the HCE1/MCS resistance to TAX. Methods HCE1/MCS was obtained by liquid overlay and rotating technique. HCE1/MCS morphological changes were observed before or after the interference of CD147 mAb. The effects of TAX on HCE1/MCS (including inhibition ratio, IC50 and index of multicellular resistance) before or after CD147 mAb treatment were determined by the method of WST-1 and the inhibition ratio curve was mapped. Cell cycle and apoptosis were detected by flow cytometer (FCM). The expression of CD147 and P-gp of both HCE1/MC and HCE1/MCS was detected by immunocytochemistry. Results HCE1/MCS was established successfully. CD147 mAb could inhibit HCE1/MCS from forming spheroids. CD147 mAb could enhance the sensitivity of HCE1/MCS to TAX. IC50 in different concentrations of CD147 mAb (5,10,20 μg/mL) HCE1/MCS group were (40.31±3.73), (32.43±1.56), and (30.69±1.01) μg/mL. CD147 mAb resulted in G1/G0 arrest in HCE1/MCS. CD147 mAb of low concentrations (0-10 μg/mL) caused a dose-dependent inhibition of HCE1/MCS (P<0.05). Combined with TAX, CD147 mAb could also induce HCE1/MCS cell cycle arrest in both G1/S and G2/M stage. The expression of CD147 and P-gp was consistent in HCE1/MCS groups. Conclusion CD147 plays an important role in muliticellular resistance of cervical cancer and inhibition of CD147 can synergistically reverse the multicellular drug resistance (MCR) in cervical cancer. The MCR of HCE1/MCS mediated by CD147 is related to P-gp.

Lipoxygenase(LOX) is related to emergence and development of many diseases, such as acute and chronic inflammation, atherosclerosis, hypertension and tumor. Therefore, the inhibition of LOX may play an important role in the prevention and treatment of these diseases. Many kinds of flavonoids, such as chalcones, flavonols, flavones and flavanols, have inhibitory effect on 5-LOX, 12-LOX and 15-LOX probably through inhibiting expression of LOX, bonding to the enzyme or reacting with free radicals generated at the active site of the enzyme. Their inhibitory activities are related to their structures. The inhibitory effects of flavonoids on LOX maybe one of mechanisms of flavonoids' some biological functions, such as anti-inflammatory, anti-tumor and reducing the risk of cardiovascular disease and cerebrovascular disease.

Objective To investigate the predictive value of spontaneous cough in extubation patients with craniocerebral in‐jury in ICU .Methods Totally 78 cases of patients with severe craniocerebral injury in ICU was divided into successful exbuation group (53 cases) and failing exbuation group (25 cases) according to the exbuation outcome .With the permission of patients ,the general clinical data ,glasgow coma scale (GCS) scores and spontaneous cough of patients were recorded .Results There were 53 cases suceed ;25 cases of patients failing in exbuation ,accounting for 32 .05% .There were no significant difference between ages , gender ,medical history ,hospital acquired pneumonia (HAP) ,disease kind between two groups (P> 0 .05) ,while had significant difference between smoking ,body mass index (BMI)、GCS scores and spontaneous cough of two groups (P<0 .05) .No significant difference was found between the conventional exbuation parameter of two groups (P> 0 .05) .Receiver operating characteristic curve (ROC) analysis showed that ,GCS scoresarea under the curve (AUC) was 0 .822 ,BMI AUC was 0 .674 ,spontaneous cough AUC was 0 .914 ,and smoking AUC was 0 .856 .Conclusion Smoking ,BMI and GCS scores and spontaneous cough times were all meaningful indices for evaluating exbuation of patients with craniocerebral injury in ICU ,in which spontaneous cough times was an important predictive factor and the most accurate one .

To introduce the basic working principle of TCL - II medical injection pump, the detailed description of the power circuit and motor drive circuit. According to the basic principle of each function, it gave the corresponding circuit diagram for peer reference. Injection pump in the maintenance can use this typical working principle and the typical electronic circuit to realize the common troubleshooting quickly and accurately.

Objective To observe the effect of heroin on EGF and IL-2 positive cells in the submandibular gland of rats. Methods Normal SD rats were divided into normal control group (NGC),saline control group (SCG)and heroin dependence group (HDG). The heroin dependent model of rats was established by subcutaneous injection of heroin, and submandibular glands were excised on the following days respectively: the 10th, 17th, 24th, 31th and 38th. Immunohistochemical SABC method, image analysis and cells calculation were used in the study. Results 1. As compared with those of the NCG, the number of EGF and IL-2 positive cells and the intensity of staining in SCG didn't change obviously. 2. During heroin dependence, the immunohistochemical staining showed that the number of EGF and IL-2 positive cells increased obviously(P

The striatum is involved in diverse behaviors which depend on intact dopaminergic innervation. Recent electrophysiological studies have revealed that dopamine alters both voltage dependent conductances and synaptic transmission, resulting in state dependent modulation of target cells. This review makes clear predictions about how dopamine should alter the responsiveness of striatal neurons to extrinsic excitatory synaptic activity.

Objective:To explore the hemodynamic effect of the modified Z Trendelenburg position in patients undergoing laparoscopic radical surgery of endometrial carcinoma, to provide a reference for the selection of the operative position of patients.Methods:A total of 80 patients who undergoing laparoscopic radical surgery of endometrial carcinoma from April 2017 to April 2019 in the Central Hospital of Xiangyang were recruited and assigned to experiment group and control group by random digits table method with 40 cases in each group. Patients in the experiment group were positioned in modified Z Trendelenburg position, while the patients in the control group were placed in conventional Trendelenburg position. The data of heart rate, mean arterial pressure, central venous pressure, systemic circulation resistance, cardiac output were recorded at 10 minutes before pneumoperitoneum (T 0), pneumoperitoneum immediately (T 1) and 5 minutes (T 2), 10 minutes (T 3), 30 minutes (T 4), 60 minutes (T 5) after pneumoperitoneum and 1 minute after recover horizontal position (T 6). Followed up investigation at 24 h after surgery, the degree of pain in the shoulder and the lower limbs were also recorded by Visual Analogue Scale(VAS). Results:The differences of heart rate, mean arterial pressure, systemic circulation resistance and cardiac output between the two groups had no statistical significance at T 0 to T 6 (all P>0.05). The central venous pressure were (9.86±1.95), (10.94±1.53), (11.33±2.24), (11.14±1.54),(7.75±1.59) cmH 2O(1 cmH 2O=0.098 kPa) respectively from T 2 to T 6 in the experiment group, lower than that of control group (18.83±2.82), (16.81±2.93), (16.27±3.56), (15.76±2.33), (8.92±1.68) cmH 2O at the same time, the differences were statically significant ( t values were 3.057-15.736, all P<0.05). The VAS scores of shoulder pain and low leg pain in experiment group were (3.48±1.10), (2.35±0.66) points, lower than that of control group (4.16±1.32), (2.99±1.02) points, the differences were statically significant ( t values were 2.404, 3.228, P<0.05). Conclusions:Modified Z Trendelenburg position can maintain the circulatory system hemodynamic stability, reduce the degree of shoulder pain and low leg pain after surgery in the laparoscopic radical resection for endometrial carcinoma patients.

ObjectiveTo observe the clinical features, complications, and prognosis of subarachnoid hemorrhage (SAH) in the aged. Methods132 old cases were analyzed retrospectively. Results56.8% of patients complained headache as the main symptoms, 43.2% with dizziness or vertigo, 40.1% with disturbance of consciousness, 70.5% with negative of meningeal irritation sign. The most common cause is the aneurysm. The mortality was more in those complicated with hypertension. ConclusionSAH in the old characterized variable clinical manifestations, no special signs, half of them with good prognosis.