Bronchial asthma is a chronic inflammation airway disease.The imbalance of T helper cell (Th) 1/Th2 immunity plays an important role in the pathogenesis of allergic asthma.Recent years a new T cell lineage has been recognized as Thl7 cells because they can produce cytokines interleukin-17.The new studies find that interleukin-17 is a negative regulator of asthma,and interleukin-17 or Th17 cells may represent an interesting therapeutic target in asthma.

Objective To investigate the relationship of serum levels of C-reactve protein (CRP) with diabetic cardiomyopathy (DCM). Method Thirty-six patients with DCM were recruited as DCM group and thirty healthy people as the control. Serum levels of CRP in all subjects were determined by immunoturbidimetry. Results Compared with control group, the serum levels of CRP in patients with DCM were singnificantly increased by about 3.7 folds[(6.95?1.37)mg/dL vs.(1.89?0.38) mg/dL, P

OBJECTIVE To investigate the pre-operation and/or pre-transfusion testing on patients to avoid risk of(occupational) infection exposure among medical staff and its significance in prevention of nosocomial infection.METHODS Serum hepatitis B(virus surface) antigen(HBsAg),antibody against hepatitis C virus(anti-HCV),(antibody) against human(immunodeficiency) virus(anti-HIV) and toluidine red unheatedserum test(TRUST) were performed on samples from 10 745 cases of pre-operation and/or pre-transfusion from 2003 to 2004.RESULTS The number and(percentage) of positive reaction for HBsAg,anti-HCV,anti-HIV and TRUST were 1163,(10.81%);21,0.20%;1,0.01%(confirmed by HIV Reference Laboratory,Center for Disease Prevention and(Control) of Zhejiang Province) and 74,(0.69%),respectively.TRUST positivity was further confirmed with TPHA.(Overlapping) infections were mostly observed between HBV and HCV,or,to a less scale,between HBV and syphilis.For HBV infection the(department) of digestive medicine was prevalent(?~2=4.77,P

Objective To evaluate the value of ultrasound-guided channel establishment in minimally invasive percutaneous nephrolithotomy(MPCNL).MethodsBetween May 2006 and December 2007,85 patients received MPCNL in our hospital.Puncture points were selected and then channels were established under the guidance of ultrasonography according to the distributions of calculi during MPCNL.ResultsUnder the guidance of ultrasonography the puncture was completed at one session in all of our 85 cases.No massive hemorrhage or other severe complications occurred.In the cases,57 cases were cured after the first operation,and 27 cases received a second surgery.One of the cases was diagnosed as having calcification of the renal papillae without kidney calculi during the operation.Residual calculi(

Objective To observe the effect and mechanism of Bush en Tiaojing Decoction (Decocti on for reinforcing the kidney and regulating the menstruation) on ovulation of p olycystic ovary syndrome (PCOS) rats. Methods Eighty fe male SD rats were made PCOS models and randomized into blank group, model group, metformin group, Bushen Tiaojing Decoction high and low dose group s, with 10 ones in each and the remainders removed. The changes of serum leptin, ins ulin and testosterone in each group were observed. Results In Bushen Tiaoji ng Decoction high and low dose groups, serum leptin, insulin and testosteron e were all decreased. The effect of Bushen Tiaojing Decoction high dose group wa s similar with that of metformin group in decreasing leptin, insulin a nd testosterone. Conclusion Bushen Tiaojing Decoction c ould improve the ovulation of PCOS rats through regulating leptin, insulin , and testosterone.

Objective To collect the information about the types and clinically-used materials for fixed partial dentures, and evaluate the current conditions and characteristics of fixed partial dentures in Shanghai. Methods Three municipal public hospitals and two private commercial dental labs in Shanghai were served as sample units. The data of fixed partial dentures from January 2005 to December 2007 were retrospectively analysed. The number, type and material of dentures of each year were observed, and the proportion of different types and materials of dentures were analysed. Results From 2005 to 2007, the total number of fixed partial dentures gradually increased year by year, and porcelain fused metal crowns accounted for the largest proportion. The application of noble crown in public hospitals increased annually, and the increase rates were 13.85% in 2005 to 2006 and 30.86% in 2006 to 2007, respectively, while the application of casting basic metal full crown decreased annually. Computer-aided design and computer-aided manufacture (CAD/CAM) fixed partial dentures (CAD/CAM crown) and implant dentures occupied a tiny percentage of total fixed partial dentures in public hospitals and privated dental labs, while both had a remarkable increase trend in number between 2005 and 2007 (227.78% and 53.01%, respectively for 2005 to 2006, and 120.34% and 27.41%, respectively for 2006 to 2007). Conclusion Fixed partial dentures are the most common prostheses for tooth defect, and are characterized by the growing application of advanced techniques and high-quality materials.

BACKGROUND:It is important to produce and save a large amount of high-activity feeder cel s for the culture of human embryonic stem cel s.
OBJECTIVE:To establish the optimal method for isolation and culture of Kunming mouse embryonic fibroblasts, and to evaluate the feasibility of preparing feeder layers for culture of human embryonic stem cel s.
METHODS:Embryonic fibroblasts were isolated and cultured by different concentrations of trypsin from Kunming mouse fetuses in vitro. The biological characteristics and growth rule of mouse embryonic fibroblasts were investigated, and then the feeder layers for human embryonic stem cel s culture were produced. The growth of human embryonic stem cel s on the prepared feeder layer was tested.
RESULTS AND CONCLUSION:The optimal fetal age for preparing Kunming mouse embryonic fibroblast feeder layer was 13.5 days. Kunming mouse embryonic fibroblasts at different concentrations grew wel with high purity and active proliferation by trypsin digestion method. There was no significant difference in the survival rate of cel s after cryopreservation for 2 weeks, 1 month, 3 months and 6 months. The cel s were proliferative from the second to fourth passage and declined sharply after the fifth passage. Human embryonic stem cel s which grew on Kunming mouse embryonic fibroblasts feeder layers were stil to remain the typical undifferentiated morphology and were strongly positive for alkaline phosphatase and periodic acid-Schiff after long-term subculture. The
mouse embryonic fibroblasts can be used as the stable and high-quality feeder cel s for human embryonic stem cel s.

Objective To investigate the resistance of clinical isolated gram negative bacilli to tigecycline.Methods One hun-dred and fifteen Escherichia coli isolates,110 Klebsiella pneumoniae isolates and 99 Acinetobacter calcoaceticus-Acinetobact-er baumannii complex isolates were collected from Affiliated Second Hospital of Zhejiang University College of Medicine from the year 2012.The other 393 A.calcoaceticus-A .baumannii complex isolates were collected from 15 hospitals of nine cities in Zhejiang province during September to October 2012.Species identification and susceptibility test were confirmed by VITEK-2 compact system,while the 393 A.calcoaceticus-A .baumannii complex strains isolated from Zhejiang province were identified by MALDI-TOF MS.Moreover,159 A.baumannii isolates with tigecycline MIC value ≥8 μg/ml or 4 μg/ml and part of the MIC value ≤0.5 μg/ml,1 μg/ml and 2 μg/ml which were firstly determined by VITEK 2 GN AST-16 Suscepti-bility card were then determined by Etest.Results The 393 A.calcoaceticus-A .baumannii complex strains were identified as 317 of A.baumannii,32 of A.pittii and 44 of A.nosocomialis.When using the FDA breakpoints,the resistance rate of tige-cycline against 115 E.coli isolates,110 K.pneumoniae isolates and 99 A.calcoaceticus-A .baumannii complex isolates were 0.0%,7.3% and 6.1%,respectively.Interestingly,85.7% of the tigecycline-resistant gram negative bacilli were resistant to carbapenems.However,only 10.0% of the carbapenem-resistant gram negative bacilli were resistant to tigecycline.Conclu-sion Tigecycline had a good activity against clinical isolated multi-drug resistant or even pan-drug resistant gram negative bacilli.No matter which criteria for tigecycline was referred to,the resistance rate oftigecycline was slightly lower by Etest than by GN AST-16 Susceptibility card.

Objective To investigate the resistance of Acinetobacter baumannii to clinical common antibiotics and new drug tigecycline. Methods Six hundred and two Acinetobacter baumannii isolates were collected from 2008 to 2009 in four teaching hospitals in Zhejiang province. Agar dilution method was used to detect the resistance of 13 clinical commom antibiotics, polymyxin B and tigecycline. Homology analysis of 24 multi-drug resistant Acinetobacter baumannii strains was used to investigate the relationship of each strain with the method of pulsed field gel electrophoresis. Results From 2008 to 2009, the Acinetobacter baumannii isolates of four teaching hospitals in Zhejiang province were mainly isolated from respiratory specimens with the number of 277 (86.0%) strains in 2009, the number of blood samples decreased from 15 (5.4%) strains in 2008 to 5 ( 1.5% ) strains in 2009, and there were no obvious change in other specimens. Acinetobacter baumannii strains were resistant to 13 clinical common antibiotics at different degree, fluctuated from 35.0% to 85.0%. Compared with the resistance in 2008, levofloxacin and tobramyxin decreased 0. 9% and 9.0% in 2009, respectively. However, the resistance of other antibiotics increased at different degree, the resistance of ceftriaxone and cefepime increased about 10.0%, and the resistance of imipenem and meropenem increased to 74.2% (239/602) and 70.8% (228/602) in 2009,respectively. Acinetobacter baumannii showed high resistance to tigecycline with the percent of 78.9% (475/602), while it was only 3.7% (22/602) for polymyxin B. There were six cloning types among the 24 Acinetobacter baumannii isolates, and the most common type was type A with the percent of 50%.Conclusions The resistance of tigecycline makes the situation of nosocomial infectious more serious. It is necessary to control the transmission of multi-drug resistant Acinetobacter baumannii immediately.

Objective To investigate the linezolid resistance mechanisms and molecular epidemiology of clinical isolates of methicillin-resistant coagulase-negative staphylococci (MRCoNS).Methods Seventeen MRCoNS,including 10 S.capitis,4 S.cohnii,2 S.haemolyticus,and 1 S.sciuri with various levels of linezolid resistance were isolated from intensive care units in our hospital from March to August 2011. Minimal inhibitory concentration (MIC) was determined by E-test method. Pulsed-field gel electrophoresis was performed to analyze the molecular epidemiology.PCRs and DNA sequencing were preformed to investigate the mechanisms of linezolid resistance in MRCoNS.Results Nine S.capitis with linezolid MIC of ＞256 μg/ml were indistinguishable,and another S.capitis with linezolid MIC of 4 μg/ml was closely related.Four S.cohnii with linezolid MIC of ＞256 μg/ml were belonged to the same clonal strain.MIC of linezolid for S.sciuri was 64 μg/ml,and were 4 μg/ml and 6 μg/ml for 2 S.haemolyticus,respectively.A commom G2576T mutation and a novel C2104T mutation were identified in 9 S.capitis with linezolid MIC of ＞256 μg/ml by DNA sequence analysis of domain V of the 23S rRNA gene.cfr gene was deteeted in all staphylococci except a S.sciuri whose 23S rRNA gene contained the G2576T mutation.Conclusion It is the first report of linezolid-resistant clinical isolates of staphylococci in China.Linezolid resistance in MRCoNS is related to the presence of DNA mutation in domain V of the 23S rRNA gene and cfr gene.It's a clonally dissemination of linezolid-resistant MRCoNS in intensive care units of our hospital.