Objective:To study the surface enhanced Raman scattering ( SERS) of safflower and identify safflower injections by SERS quickly and effectively. Methods:Through comparative analysis of the Raman spectroscopy of safflower injections and the corre-sponding control herbs, the rapid identification of safflower injections was realized. Results:The results showed that several character-istic peaks of safflower were enhanced obviously in SERS, which could be used to identify safflower injections. Conclusion:The meth-od is reliable, rapid, accurate and specific, which can be applied as a method to identify safflower and its injections.

Animals ; Cattle ; Humans ; Infant ; Infant Formula ; Milk Hypersensitivity ; Milk Proteins ; adverse effects

Objective To investigate the changes of protein C and fibrinolytic system in patients with acute cerebrovasculer disease and the influence of intervened treatments on acute cerebral infarction(ACI).Methods Determinations of protein C(PC),protein S (PS),thrombomodulin(TM),Tissue-type plasminogen activater(t-PA),plasminogen activator inhabitor(PAI) were performed in patiens with acute cerebrovasculer disease and normal control(NC),then redeterminated these items after treatments in patients with ACI.Results PC,PS:The levels in patients with ACI were significantly lower than that in other groups.After dreatments,the levels in patients with ACI increased.There were no significantly discrepancy in acute cerebral hemorrhage(ACH) and lacunar cerebral infarct(LCI) compared with NC;TM:The levels in patients with ACH,ACI were significantly higher than that with LCI and NC.After dreatments,the levels in patients with ACI decreased;t-PA:The levels in patients with ACI were lower than NC,whereas the levels in patients with ACH increased.After dreatments,the levels in patients with ACI increased;PAI:The levels in patients with ACI were significantly higher than NC,and also found that the levels in patients with ACH and LCH were higher compared with NC.The levels in patients with ACI decreased after dreatments.Conclusions There are obviously abnormalities in plasma coagulative and fibrinlytic parameters in acute cerebrovasculer disease;To check the levels of PC,PS,TM,t-PA,PAI are important to predict the type of stroke,the seriousness of disease,the effect of treatment,prognosis of the cases and suggest that earlier treatments be important.

The severity of coronary artery diseases is assessed based on morphological and functional stenosis.The study Oil detection of coronary artery diseases based on morphological lesions has unveiled its short comings for evaluation of the seriousness of the disease.Therefore,many research groups diagnose the coronary artery diseases by functional stenosis.From functional aspect,studies of x-ray angiography algorithms for blood flow and velocity measurement are potentially clinically important and are an actively researched subject,which Can accurately diagnose the coronary diseases from functional stenosis aspect.Based on the angiographic images,the article describes formulation and characteristics of existing coronary blood flow and velocity techniques such as tracking and computational methods.Tracking methods can be divided into four classes:bolus transport time algorithms,continuous velocity determination from two-dimensional(time-distance)parametric images,contrast traversal distance algorithms,and droplet tracking algorithms.Computational methods can be divided into six classes:indicator-dilution or Stewart-Hamilton method,first-pass distribution analysis,inverted continuity equation algorithms,inverse mass transport problem approach,optical flow type algorithms,and the fluid continuity method.

Objective To describe the Evidence-based Nursing Competences of nurses who have received Evidence-based Nursing training in tertiary hospitals in Shanghai and analyze its influencing factors. Methods Use the Evidence-based Nursing Competency Rating Scale made by Wang Yilei to investigate 428 nurses who have received Evidence-based Nursing training in 9 tertiary hospitals in Shanghai. Results The average score was (54.39±16.77), the 3-scoring rate was 25.1%-64.3%and the 4-scoring rate was 2.8%-23.1%which indicates the nurses had moderate-low level of EBNP competence after training project including in service training, seminar, lecture, workshop and so on, but still at a low level. Educational background and training time were the main influencing factors (P<0.05). Conclusions Nurses in tertiary hospitals in Shanghai have a moderate-low level of evidence-based nursing practice competence after education project. Comprehensive evidence-based nursing training system is expected.

Objective To investigate the effects of 1,25-dihydroxyvitamin D3 in the prevention of corneal allograft rejection. Methods Forty-five SD rats (only right eyes) were used as recipients,and 15 Wistar rats (double eyes) were used as donors. SD rats were randomly divided into 3 groups: the autograft control group,the allograft control group (receiving peanut oil as placebo),and the allograft group were treated with 1,25-dihydroxyvitamin D3 (1.0 ?g.kg-1.d-1,3 times/d for 13 d,i.p.). Drugs were injected intraperitoneally for 14 d after transplantation. Routine antibiotic eye drops were used. Grafts were observed by operating microscopy everyday after transplantation. Five SD rats in each group were killed respectively at the 14,21,and 30 d postoperatively. Neovascularization and inflammation were evaluated with HE staining. ELISA was used to detect the contents of IL-1?,IL-2,IL-8,and IL-10 in the peripheral blood. Results The mean survival time (MST) of the graft was (21.7?6.8) d in the autograft control group,(11.2?2.5) d in the allograft control group,and (19.3?5.2) d in the allograft group. There was statistically significance between the latter 2 groups (P

Objective To study the effect of letrozole (LE) on ovulation in patients with polycystic ovary syndrome (PCOS).Methods One hundred and sixty-four patients with PCOS were divided into LE treatment group (n=68) and chloramiphene (CC) treatment group (n=96).Growth rate of follicles,serum hormone level,endometrial thickness,and cervix mucus were observed before and after treatment.Results The growth rate of follicles was 1.67 and 1.78 mm/d,respectively,in LE and CC treatment groups (P=0.14).The serum T level on the day of LH peak was 2.1?1.9 and 2.0?1.7ng/dL,respectively,in LE and CC treatment groups (P=0.93).The serum testosterone (T) level on day of LH peak and on day 3 of menses was 2.1?1.9 and 1.4?1.5 ng/d,respectively,in LE and CC treatment groups (P=0.09).The serum E2 level on day of LH peak was 89.0?59.5 and 108.1?46.0 pg/ml,respectively,in LE and CC treatment groups (P=0.13).The endometrial thickness was 11.3?1.6 and 9.7?1.4 mm,respectively,in LE and CC treatment groups (P=0.07).The score of cervix mucus was 11.0?1.1 and 8.3?3.1,respectively,in LE and CC treatment groups (P=0.003).Conclusion Letrozole can effectively induce ovulation in patients with polycystic ovary syndrome,and does not increase serum T level.

Objective To build occupational management skills training mode for the community patients with psychosis in recovery stage by using action research method. Methods Action research method was applied to mental rehabilitation patients in Sunshine Psychiatric Garden of Shanghai to manage vocational rehabilitation support. By literature verification and empirical experience, the key problems were found out, and goals were established. Then management skills according to planning, action, observation, reflection and correction were implemented. This circle were spiraled for 2-3 times stages, and verification and evaluation before and after action practice based on Negative Symptom Rating scale (SANS) and Social Disability Screening Scale (SDSS). Results The community mental vocational rehabilitation was built with action research and based on the evidence of foreign psychic rehabilitation supportive individual placement and support. The mode should consider the professional characteristics of technical, social and economic. Also, it should contain professional ability, social skills and job adaptability training and performance evaluation, and offer salary allowance. The factors of SANS in 21 patients after practice were significantly improved (P < 0.01), and most of the factors in the SDSS were significantly improved (P < 0.01). Conclusions Community psychic vocational rehabilitation using action research method is a kind of advanced nursing practice, and also a process of evidence-based practice, which can provide a systematic, specific, practical work method for community mental rehabilitation institutions to force vocational rehabilitation training.

Sepsis is a major problem in the pediatric critical care medicine.The related research indi-cates that the nutrition risk may be one of the risk factors that affect the incidence and mortality of sepsis. Through nutritional risk screening and assessment,it can be used to provide reference for assessing the nutri-tional risk,the nutritional support and the effect of nutritional support on prognosis of children with sepsis.At present,there are few studies on the nutritional risk and nutritional support in children with sepsis.In this arti-cle,we reviewed the research progress on nutritional risk and nutritional support in children with sepsis.

Objective To explore the relationship between calcium channel ?1 subunit (Cav1.1) gene intron 26 -67 A/G polymorphism and thyrotoxic periodic paralysis(TPP). Methods Cav1.1 gene polymorphism at position -67 was determined by polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) in 46 male patients with TPP, 68 male Graves’ disease (GD) patients without TPP and 72 healthy male controls. The difference of genotype and the variation of allele frequencies were analyzed by Chi-square test. Results (1) Frequencies distribution of AG+GG genotype in TPP, GD and control groups were 47.83%, 14.71% and 29.17% respectively, and those of allele G were 44.57%, 13.24% and 27.78% for the three groups respectively. (2) Frequencies of -67 AG+GG genotype in TPP group were significantly higher than those in GD and CON group(OR=5.32, P