Objective To study the role of NF-?B pathway in HGF (hepatocyte growth factor)-mediated proliferation of WB F-344 cell. Methods WB F-344 cells were treated with HGF in different concentrations i.e. 0, 20, 40, 80 and 100ng/ml. After incubation for 24 hours, proliferation effect was analyzed with [3H] Thymidine. WB F-344 cells transfected with I?B? plasmid mutants by liposome transfection were used to block NF-?B activity, and then they were treated with 40ng/ml HGF for 0, 15, 30, 60 and 120min, respectively. The nucleoprotein was extracted to determine the NF-?B DNA-binding activity by EMSA. WB F-344 cells were also transiently transfected with BD Great EscA PeTM SEAP vector with liposome and then stimulated with HGF in different concentrations (0, 10, 20, 40ng/ml) for 8 hours to detect the NF-?B transcription activity. WB F-344 cells were pre-treated with NF-?B inhibitor BAY-11-7082 or ASA, and then with 40 ng/ml HGF for 24 hours to analyze the proliferation effect by [3H] Thymidine. Results HGF promoted the proliferation of WB F-344 cells and exhibited positive dose-effect tendency. HGF could enhance the NF-?B DNA-binding activity. NF-?B complexes appeared 15min after addition of HGF. The complexes reached the peak value 30-60min after addition of HGF and then decreased gradually. HGF could also enhance the NF-?B transcription activity, exhibiting positive dose-effect tendency. HGF-mediated proliferation was remarkably inhibited when NF-?B activity was blocked by BAY-11-7082 or ASA. In WB F-344 cells transfected with p-CMV-I?B?, the proliferative response to HGF was completely interrupted. Conclusions HGF may promote the proliferation of WB F-344 cells in a positive dose-effect tendency. The activation of NF-?B is necessary for HGF-mediated proliferation of WB F-344 cells.

The operational main body,fund source,information source and its assessment,service contents and forms of the major medical and health information service platforms for the public in United Kingdom,USA,Canada and Australia were compared and analyzed with suggestions put forward for establishing government-led non-profitable medical and health information service platforms,namely enlarging the depth and width of medical and health information service,promoting the assessment of information service on the platforms by the third party,enforcing the management of medical and health information service platforms,improving the interaction and feedback between website maintenance staff and users,and strengthening the construction of digital medical service system.

The effects of immediate administration of inderal following acute myocardial infarction on left ventricular remodeling were studied in acute myocardial infarction model of the rabbit. The results showed that there was no significant difference in the infarct size and expansion index between the infarct-inderal group and the infarct-placebo group. As compared with sham surgery-inderal group，infarct-inderal group had a higher ventricular weight，left ventricular volume index and left ventricular wall stress ［(2.65±0.17) g/kg vs (2.32±0.11) g/kg，P<0.05;(2.22±0.23) ml/kg vs (1.70±0.12) ml/kg，P<0.001;(0.84±0.07) vs (0.75±0.052)，P<0.05，respectively］，but there was no significant difference between the infarct-inderal group and infarct-placebo group. Heart rate in the infarct-inderal group was significantly decreased as compared with that of infarct-placebo group ［(153±6.36) min－1 vs (225±4.08) min－1，P<0.001］，but there was no significant difference in left ventricular end-diastolic pressure，+dp/dt max and cardiac index between the two groups. It was suggested that immediate administration of inderal postinfarction had no preventive effect on left ventricular remodeling.

Objective To investigate the possibility of gestational body weight intervention and its influence on pregnancy outcomes.Methods 300 healthy primiparae with normal pregestational BMI and living monoeyesis and head presentation were randomly divided into the observation group (n = 120) and control group ( n = 180).Both groups were received general health education and regular obstetric examination, in addition, the observation group received body weight intervention.The data about changes in BMI and body weight from pregestation to ante partum were collected and pregnancy outcomes were assessed.Results The incidence of △ BMI≥6,gestensive hypertension, diabetes, cesarean section,large-for-date infant,intrauterine fetal distress,postparum hemorrhage in the observation group was significantly lower than those of the control group( x2 = 19.265,4.903,6.875,13.522,7.354,4.545,3.993, all P < 0.05).Conclusion Body weight intervention had a preferable feasibility and could improve pregnancy outcome.

Objective:To explore the multiple meditation effects of emotional intelligence and self-esteem between interpersonal initiative personality and social positive adaption in left-behind junior high school students.Methods:Totally 331 left-behind junior high school students [165 boys,160 girls,average age of (14.5 ± 1.2) years] were selected.They were assessed with the Interpersonal Self-Support Scale for Middle School Students (ISSS-MSS),Emotional Intelligence Questionnaire (EIQ),Rosenberg Self-Esteem Scale (RSES) and Social Adjustment Scale (SAS-YB).Results:The scores of social positive adaption were positively correlated with the scores of interpersonal initiative personality,EIQ and RSES (r ＝ 0.47-0.67,Ps ＜ 0.001).Path analysis showed that interpersonal initiative personality had direct effects on social positive adaption (β ＝ 0.47,P ＜0.001).Mediation modeling showed that interpersonal initiative personality could exert effects on social positive adaption indirectly through the mediating effect of not only emotional intelligence,but also self-esteem (x2/df＝ 3.03,TLI ＝ 0.87,CFI ＝ 0.89,RMSEA ＝ 0.08).Conclusion:The present study indicates that parallel multiple mediation model of emotional intelligence and self-esteem could explain the mechanism of influence of interpersonal initiative personality on social positive adaption appropriately.

OBJECTIVE: To evaluate the cost-effectiveness of five regimens of antibacterial drug therapy in the treatment of community acquired pneumonia. METHODS: A retrospective study was conducted, in which a total of 128 cases with pneumonia were enrolled and randomly assigned to receive each of the following 5 therapies,cefoperazone sodium/ sulbactam sodium(Group A),levofloxacin(Group B),amoxicillin/ clavulanate potassium(Group C),azithromycin plus ceftazidime(Group D),and cefoxitin(Group E),the therapeutic effects were monitored and the cost-effectiveness analyses were conducted on the five groups. RESULTS: The costs of the five regimens were 2 137.0 yuan, 1 393.0 yuan, 1 152.5yuan, 2 044.2 yuan and 1 827.0 yuan, respectively;The effective rates were 85.2%, 86.6%, 80.6%, 89.6% and 78.5%, respectively;The cost-effectiveness ratios were 25.08 , 16.09, 14.30, 22.82 and 23.27, respectively; As compared with group C, the incremental cost-effectiveness ratios of the other four groups were 214.02,40.08, 99.08 and —321.19, respectively. CONCLUSION: The therapy of amoxicillin/ potassium clavulanate is superior to the other regimes in the treatment of community acquired pneumonia.

Objective To study the expression of connective tissue growth factor(CTGF) and PEST containing nuclear pro‐tein(PCNP) and their significance in pancreatic cancer .Methods The expressions of CTGF and PCNP proteins were tested by im‐munohistochemistry and Immunofluorescence in 39 cases of pancreatic carcinomas and adjacent paracancerous tissues .Results The positive rate of CTGF and PCNP in pancreatic carcinomas was significantly higher than adjacent paracancerous tissues(χ2 =60 .41 , 51 .46 ,all P<0 .01) .The differences of the expression of CTGF and PCNP in pancreatic carcinoma of tumor differentiation ,TNM stage and lymph node metastasis was significant (P< 0 .05) .Conclusion The expression of CTGF and PCNP in the pancreatic cancer was obviously increased ,and the level of CTGF ,PCNP had remarkable connection with the stages of tumor and the condition of lymph node metastasis .

There is a close relationship between oxidative stress induced tissue damage and many diseases.As a multi-function oxidase,lipoxygenase can generate reactive oxygen species (ROS)by oxidatively metabolizing various endogenous and exogenous chemicals such as drugs and environmental pollutants.Lipoxygense can catalyze endogenous che mical reaction,such as arachidonic acid and pro-duce ROS.Lipoxygenase can be also activated in other ways to generate ROS by activating signal trans-duction.Moreover,some exogenous chemicals can be metabolized into highly reactive radical intermedi-ates by lipoxygenase,inducing ROS generation.The accumulation of intracellar ROS can damage intra-cellar redox balance and induce oxidative stress.That may be one of the possible toxic effect mecha-nis ms of che mical agents.

Objective To evaluated the treatment results of late course accelerated fractionation radiotherapy for Stage Ⅲ and Ⅳa nasopharyngeal carcinoma. Methods 100 patients with stage Ⅲ and Ⅳa nasopharyngeal carcinoma were randomized into two groups. The late course accelerated hyperfractionated radiotherapy combine chemotherapy group(LCAF+CT group) received the induction chemotherapy for two cycles, followed by conventional fractionation radiotherapy to a dose 40 Gy, than changed into accelerated hyperfractionated radiotherapy to a total dose of 70 Gy, and use two cycles adjuvant chemotherapy, The conventional fractionation radiotherapy combine chemotherapy group(CF+CT group) received the chemotherapy that it similar to the LCAF+CT group, and use the conventional fractionation radiotherapy to a total dose of 70 Gy. Results The 3- and 5-year overall survival rate were 70.0% and 62.0% respectively in LCAF+CT group and 58.0 % and 46.0 % respectively in CF+CT group, had the significant difference (P 0.05). There were the cranial nerves complications in two patients of LCAF+CT group. Conclusions The results from this study show that the 3- and 5-year overall survival rate, the 3- and 5-year disease free survival rate and the 3- and 5-year local-regional free survival rate in LCAF+CT group was significant better than in CF+CT group, but the radiation-induced damage to the cranial nerves was improved in LCAF+CT group.

General biology is a preclinical course for seven-year program students.It is important for students to grasp the fundamental ideas in the course.Holding contests of lecturing on biomedicine in postgenome era is a good application of the teaching-and-learning-through-interest technique.It aims at broadening students’vision,making students familiar with cutting-edge technologies in biology and enhancing their ability to search and read literatures,and so on.