AIM: To study the effects of oxidized high-density lipoprotein (oxHDL) on the expression of monocyte chemoattractant protein-1(MCP-1) and intercellular adhesion molecule-1(ICAM-1) and intracellular free calcium concentration （［Ca2＋］i） level in cultured human umbilical venous endothelial cells(HUVECs). METHODS: The MCP-1 protein content in the medium of conditioned HUVEC was measured by ELISA, and the ICAM-1 on HUVECs was detected by indirect immunofluorescence, and ［Ca2＋］i was determined by Fluo-3/AM, the injury of cells was observed by scanning electron microscopy (SEM).RESULTS: oxHDL could induce the expression of MCP-1 and ICAM-1 in HUVECs. In oxHDL group (HUVECs were incubated with 100 mg protein/L oxHDL for 24 h), the levels of MCP-1, ICAM-1 and ［Ca2＋］i increased by 160%, 60% and 70% respectively compared with the control group (P＜0.01). When HUVECs were incubated with 300 mg protein/L oxHDL for 24 h, cells were injured obviously. CONCLUSION: By inducing the expression of ICAM-1 and MCP-1 in endothelial cells, oxHDL may promote monocyte-endothelium adhesion and monocyte migration to intima, it may promote atherosclerosis as oxidized low-density lipoprotein (oxLDL).

Objective To investigate the relationship of the serum anti-lysosome associated membrane protein 2 (LAMP-2) antibody levels and anti-neutrophil cytoplasmic antibodies (ANCA)-associated glomerulonephritis.Methods Thirty-three patients with new onset ANCA-associated glomerulonephritis and thirty healthy controls were enrolled.ANCA detection was performed using indirect immunofluorescence (IIF).Enzyme-linked immunosorbent assay (ELISA) was used to detect myeloperoxidase (MPO),proteinase-3 (PR3) and other ANCA-associated antibodies including LAMP-2.The cut-off value of the serum anti-LAMP-2 antibody was determined by a receiver operating characteristic (ROC) curve.Results The serum levels of anti-LAMP-2 antibody in new onset ANCA-associated glomerulonephritis patients were significantly higher than remission stage ANCA-associated glomerulonephritis patients and healthy controls (P ＜ 0.05).The serum levels of anti-LAMP-2 antibody showed no visible difference between the remission ANCA-associated glomerulonephritis patients and healthy controls (P ＞ 0.05).The levels of anti-LAMP-2 antibody showed a strong positive correlation with ESR,Scr,BUN,proteinuria,crescent proportion and Birmingham vasculitis activity score (BVAS) and a negative correlation with Ccr,Hb and Alb.Conclusions Anti-LAMP-2 antibody is correlated with the activity of ANCA-associated glomerulonephritis and the severity of renal damage.It may be a useful indicator on the activity of ANCA-associated glomerulonephritis.

With the improvement of survival outcome by modern surgical treatment,more and more attention has been paid to the postoperative quality of life.As known,the most related factor of postoperative quality of life is the pattern of digestive tract reconstruction.Current evidences indicate that Roux-en-Y esophagojejunostomy + jejunal pouch has some advantages in total gastrectomy and Roux-en-Y gastrojejunostomy might be most suitable reconstruction in distal gastrectomy.Pylorns-preserving gastrectomy and proximal gastrectomy are only considered in early gastric cancer,i.e.predictive cTlcN0.Pylorus-preserving gastrectomy does not exactly superior to distal gastrectomy.Total gastrectomy with Roux-en-Y esophagojejunostomy is superior to proximal gastrectomy with esophagogastrostomy.For unresectable lower tumor with gastric outlet obstruction,gastric cancer Devine exclusion + gastrojejunostomy might be better than simple gastrojejunostomy,while stent placement is safer and suitable for predictively short-term survival,poor performance status or senility patients.However,the current available evidences of reconstruction in gastric cancer surgery is still poor in quality and more high-quality large-scale multi-center randomized controlled trials are required to resolve the controversies.

Objective To evaluate the effect and safety of transobturator inside-out tension-free urethral suspension (TVT-O) as the surgical treatment on post-operational recurrence of female stress urinary incontinence (SUI). Methods Eleven women with SUI recurrence who underwent TVT-O were selected in the research. Their incontinence recurred from 1 months to 8 years after the previous surgical treatment.Results The operative time was (28 ±9) min, the bleeding volume was (15 ±5) ml. Postoperative complications: urinary retention in 1 case, vaginal erosion in 1 case. Follow-up was enforced for 6-36months, median time was 16 months. Grouts-Blaivas analogue scale:among the 11 patients, 9 patients were cured, 2 patients were improved. ICI-Q-SF score: preoperative 13-21 ( 15.36 ± 2.34) scores compared to postoperative 0-9 (1.46 ±3.27) scores (t =5.850,P ＜0.01 ). I-QOL score: preoperative 23.33-36.67(29.85 ± 4.74) scores compared to postoperative 66.67-100.00 (95.15 ± 10.12) scores (t = 17.753,P ＜0.01 ). PISQ-12 score of 10 patients: preoperative 16-25 (20.10 ± 3.07) scores compared to postoperative 11-20( 14.00 ± 2.83 ) scores (t = 11.600, P ＜ 0.01 ). Conclusion If conservative treatments fail, TVT-O is highly effective and safe for the postoperative recurrent SUI.

Pancreatic cancer is a kind of highly malignant aggressive cancers.Perinearal growth is one of the importcant biological characteristics of pancreatic cancer.Perineural invasion is an independent prognostic indicator of its prognosis.However,the mechanism has not yet completely clear.The pancreatic cancer perineural invasion mechanism research has been the focus of scholars to discuss,however it' s difficult to break through.Therefore,the discussion pancreatic violation of the peripancreatic neural mechanism for early clinical find,early treatment is essential.The mechanisms of pancreatic cancer research,from all kinds of adhesion molecule to the nerve growth factor,related ligand protein path to susceptibility gene expression,have been reported largely,which helps enhance people' s awareness of peripancreatic neural invasion.At the same time,perineural invasion in the early detection of disease,prolonging survival period and improving the quality of life has important significance.Now the progresses of reviews in recent years are discussed as follows.

Objective To investigate the effect of tonic pain stimulation on the attention network test (ANT) . Methods Twenty healthy males recruited from university were enrolled in this study, and laboratory pain was induced by cuff pressure stimulation on the upper arm. All subjects performed the task separately under pain, no-pain and pressure conditions. The reaction time and accuracy rate of the ANT were quantified. The subjective pain perception was assessed by the short-form McGill Pain Questionnaire ( SF-MPQ) . Results Across three conditions, the executive network showed significant pain effect (F = 76.31 , P< 0.001) : no-pain condition at (92.51 ±35.71 )ms, pressure condition at (94.06 ±25. 28) ms, pain condition at ( 120.24 ± 18.21 )ms. Furthermore, the average reaction time and the A-scores (i. e. affective words) in SF-MPQ caused by pain was positively correlated ( r = 0.11, P = 0.03) . Conclusion Pain can result in deficit in the executive network for attention regulation. The negative affections caused by pain can interfere with attention.

Acupuncture Therapy ; instrumentation ; methods ; Aged ; Female ; Humans ; Male ; Middle Aged ; Periarthritis ; physiopathology ; therapy ; Range of Motion, Articular ; Shoulder Joint ; physiopathology ; Treatment Outcome

This paper reported one case with deposition of schistosome eggs in lymph nodes of rectal cancer draining area.

OBJECTIVE: To study the effects of Azone,propylene glycol(PG),oleic acid(OA) alone or in combination of two or three as penetration enhancers on the in vitro transdermal efficacy of diclofenac potassium(DP) gels.METHODS: Thirteen formulations of DP gels containing the following different penetration enhancers at different concentrations were prepared: blank,3% Azone, 5% OA,12% PG,6% PG+2.5% OA,12% PG+5% OA,1.5% Azone+2.5% OA,1.5% Azone+6% PG+5% OA,1.5 % Azone+12% PG,3% Azone+5% OA,3% Azone+6% PG,3% Azone+6% PG+5% OA,and 3% Azone+12% PG.The in vitro permeation test was performed on a modified Franz-type diffusion cell with human skin as transdermal barrier taking transdermal velocity(J) as index to determine and compute the transdermal properties of the DP gels after treated respectively by the above 13 penetration enhancers.RESULTS: Compared with the blank control group,all the penetration enhancer-treated formulations showed significant higher J value,with the formulation treated by 3% Az-one+12% PG enhancer showing the highest J value of 10.253 0 ?g?cm-2?h-1.CONCLUSION: All the three penetration enhancers could promote the transdermal permeation DP gels,much as in the combination of Azone and propylene.