Objective To investigate the clinical application value of fluorescence PCR in vitro diagnosis(IVD)reagent kits in the HLA-B27 detection by comparing 2 kinds of HLA-B27 IVD reagent kit approved by CFDA.Methods A total of 573 clinical blood samples were collected and detected for HLA-B27 by the approved reagent kits based on the fluorescence PCR technique and the flow cytometry.The samples with inconsistent testing results by the two kits were further confirmed by the PCR sequencing.At the same time,about 5% samples of the positive results detected by the fluorescence PCR method were extracted for conducting the re-testing.Results Among 573 samples,191 samples were HLA-B27 positive and 382 cases were HLA-B27 negative by flow cytome-try;the same samples had 194 cases of HLA-B27 positive and 379 cases of HLA-B27 negative by real-time PCR.With flow cytome-try as reference of the final results,the positive coincidence rate of the two kinds of kit was 96.33%(184/191),the negative coinci-dence rate was 94.76%(362/382),27 samples had inconsistent results from the two kinds of assay(accounting for 4.71% of the to-tal number of samples),the total coincidence rate was 95.29% [(184+362)/573],the Kappa value was 0.896(P =0.02);the chi-square test P =0.021,the two kinds of testing method had the high consistency,but the differences existed in the testing results. The re-testing results by PCR sequencing(including 27 samples with inconsistent results by two kinds of kit)were entirely consist-ent with the fluorescence PCR testing results.Conclusion Compared with the authority method flow cytometry for HLA-B27 tes-ting in clinic,the fluorescence PCR kit may present more accurate judging ability for the HLA-B27 testing on the basis of ensuring the higher consistency of the testing results,is easier compared with the sample preparation and operating procedures,and has the stronger clinical application value and prospects s.

Selenium, iodine and zinc are the essential trace elements for human body and the important components of many kinds of hormones, proteins and enzymes. The three elements can play important biological roles by affecting the hormones, proteins and enzymes in human body, one of the three elements can influence the biological effects of the other two. For preventing and curing some deficiency diseases and supernutrition, to study the correlated biological effect of the three trace elements will demonstrate the important theory significance and practical value.

BACKGROUND:Interleukin-1βand matrix metal oproteinase-13 can promote the metabolism of chondrocytes, inhibit the capacity of synthesizing and repairing, induce the degradation of extracellular matrix, and play a crucial role in the occurrence of osteoarthritis.
OBJECTIVE:To observe the effects of extracorporeal shock wave therapy on the interleukin-1βand matrix metal oproteinase-13 expression in rabbits with experimental knee osteoarthritis.
METHODS:Thirty New Zealand rabbits were randomly and equal y divided into treatment group, model group and control group, with 10 rabbits in each group. Model of knee osteoarthritis was established in both the treatment group and model group, using modified plaster cast in extension position for 6 weeks. Then the rabbits of treatment group were treated with extracorporeal shock wave therapy, each 1 000 impulse, at the energy flux density of 0.1 mJ/mm2. There were no treatments in the control group. The rabbits in each group were sacrificed at 4 weeks after treatment, the knee synovial fluid and articular cartilage were col ected from the rabbits. The pathological changes of knee joint were detected using hematoxylin-eosin staining and toluidine blue staining. The interleukin-1βand matrix metal oproteinase-13 expression in the synovia was detected using ELISA and immunohistochemical staining respectively.
RESULTS AND CONCLUSION:The interleukin-1βconcentration in the synovial fluid was significantly higher in the treatment group and model group than the control group (P<0.01), and the treatment group after treatment showed a lower concentration than the model group (P<0.05). Mankin scores in treatment group and model group were significantly increased compared with the control group (P<0.01), and the treatment group after treatment showed a lower score than the model group (P<0.05). The interleukin-1βand matrix metal oproteinase-13 positive expression rates in the treatment group and model group were significantly increased compared with the control group (P<0.01), and the treatment group after treatment showed a lower rate than the model group (P<0.05). The extracorporeal shock wave therapy can downregulate the expression of interleukin-1βand matrix metal oproteinase-13, promote the synthesis of new col agen.

Objective To research the growth of human umbilical vein endothelial cell(HUECV) cultured with different dose of selenium. Methods HUVEC were cultured with selenium in various concentration (50、100、500、1000、2000 nmol/L) for 48 h in vitro. The morphological changes of cells were observed, activity of cell was measured by MTT method, and at the same time the levels of SOD, GSH-Px, MDA, NO, iNOS, eNOS in the culture were determined. Results At the doses of 1 000-2 000 nmol/L, the cells were damaged and revealed related morphological change. Cell activity was significantly higher at the dose of 100 nmol/L compared with that of control (P

Objective:To investigate affection of ureaplasma urealyticum (UU) infection on the production of calculus of the urinary and reproductive system in male rat. Methods:Sprogue-dawley(SD) rats were infected with UU4 (serotype 4) through repeated natural sexual intercourses for 8 weeks. The urinary and reproductive system were detected . Results:Twelve point five percents of rate infected with UU had soft calculus in urinary tracts, while 27.5 percents of rate infected with UU had soft or hard calculus. Conclusion: Infection with UU may lead to the production of calculus in urinary and reproductive system in male rats

Objective To explore the effect of extracorporeal shock wave (ESW) on expression of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in cartilage in rabbits with knee osteoarthritis. Methods 30 rabbits were randomly divided into control group, model group and treatment group, with 10 rabbits in each group. Model of knee osteoarthritis was established with modified plaster cast in extension position for 6 weeks except the control group, and the treatment group was treated with ESW (each 1000 impulse, the energy flux density of 0.1 mJ/mm2). The rabbits were sacrificed respectively 4 weeks after ESW treatment, the general and histological changes of articular cartilage were examined and immunohistochemical staining was used to observe the expression of IL-1β and TNF-α in cartilage. Results The expression of IL-1β and TNF-α were significantly lower in the treatment group than in the model group (P<0.01). Conclusion ESW can down-regulate the expression of IL-1β and TNF-α in chondrocytes with osteoarthritis.

ObjectiveTo evaluate the role of transurethral resection of the prostate (TURP) in the management of prostate carcinoma.MethodsTURP were randomly performed on 19 suspected patients with prostate carcinoma, based on their mean serum PSA level (14.5 ng/ml) and negative result of anal touch,so as to take more biopsy samples and to make histopathologic diagnosis.Meanwhile,another 8 patients with prostate carcinoma underwent palliative TURP so as to resolve bladder outlet obstruction.ResultsOf the 19 suspected patients, 6 (33%) were pathologically diagnosed to have prostate carcinoma.These 6 patients were all alive during a follow-up for 3 months to 6 years.Obstructive symptom was relieved in 4 patients who underwent palliative TURP,but repeat palliative TURP was needed in 2 patients 3 months after initial operations.ConclusionsIn certain circumstances,TURP may play a role in the diagnosis of prostate carcinoma. In addition, it relieves obstructive symptom caused by enlarged prostate. However, the long-term effects are unpredictable.

Ubiquitination on target proteins is the signal of cellular protein degradation.Ubiquitin ligase E3 is one of the key enzymes in ubiquitination,it recognizes a specific substrate protein and recruits an ubiquitin conjugating enzyme E2,mediating the ubquitin transfer from the E2 to the substrate protein.Ubiquitin ligase E3 can be divided into two subfamilies according to their different structure characters and function mechanisms,the HECT(homologous to E6AP C terminus) family and the RING-finger family.Members of the HECT E3 share the common HECT catalytic domain,which can bind to an E2 and load the ubiquitin on themselves before catalyzing the transfer of ubiquitin to the target proteins.While the RING-finger E3 all contain an similar E2 binding domain and a unique substrate binding part,mediating direct ubiquitin transfer from the E2 to the substrate.The most recent progresses in the stuctural and functional studies of these two E3 famlies were summarized.

Objective To enhance the rapid response capability of urgent medical unit to satisfy the requirements of natural and manmade disasters for medical service.Methods Modularization principle and system approach were applied.Results Modularized design and management of urgent medical equipments were realized.Conclusion It's very important to realize modularized management of urgent medical equipments.

Objective To discuss the clinical value of transesophageal echocardiography used in extracorporeal circulationcardiac valve replacement. Methods 89 patients received extracorporeal circulation cardiac valve replacement in our hospital from January 2012 to December 2014 were included in the study. Transesophageal echocardiography were performed preoperatively, intraoperation and postoperatively and the findings were compared to the result of preoperation transthoracic echocardiography exam. The measurement of aortic annulus diameter and detection rates of calcification obtained by transthoracic and transesophaged echocardiography were compared. Changes in heart function parameters before and after operation were compared. The alternations in surgery plan made after pre-operative transesophageal echocardiography exam and any relevant treatment or procedure performed according to intra-operation transesophageal echo findings were recorded. All the patients were followed up for 6 months - 40 months. Results Before extracorporeal circulation cardiac valve replacement, the aortic annulus diameter measured and detection rates of calcification by transthoracic echocardiography were smaller and lower than actual surgical pathology results ( P < 0. 05 ) . No differences in the results from transesophageal echocardiography of two-dimensional and three-dimensional examination when compared to pathological findings ( P > 0. 05 ) . Conclusions For patients receiving extracorporeal circulation cardiac valve replacement, transesophagus echocardiography examination is helpful to guide the selection of valve stent and operation methods.