Biomarkers, Tumor ; analysis ; Breast Neoplasms ; metabolism ; pathology ; Female ; Guidelines as Topic ; Humans ; Immunohistochemistry ; methods ; In Situ Hybridization, Fluorescence ; methods ; Quality Control ; Receptor, ErbB-2 ; metabolism

Biopsy, Large-Core Needle ; Coloring Agents ; Hematoxylin ; Humans ; Immunohistochemistry ; Lymphoma ; pathology ; Pathology ; standards ; Pathology, Molecular ; Staining and Labeling

Objective To evaluate the effect of the miracle questions in solution?focused brief therapy on memory tend and emotional stage in a non?clinical situation in college students. Methods A quasi?experimental design was adopted,and two kinds of materials for self?coaching( solution?focused,problem?focused) were devel?oped according to the previous researches. Seventy?two subjects were randomly divided into two groups,receiving the miracle questions in solution?focused brief therapy( The miracle question group,thirty?six subjects) or problem?focused coaching intervention(problem?focused group,thirty?six subjects).Exception memories number and positive and negative emotion level were evaluated between the two groups. Results The subjects receiving the miracle question group write more exception memories (3.67±2.07) than problem?focused group (1.61±1.18) ( t=5.178, P<0.01) . There was no significant difference in the positive and negative emotion level between the two groups(P>0.05) . Conclusion The miracle questions in solution?focused brief therapy can trigger the subjects to exceptional memories .This suggests that in practical application,questions order has impact on the effect of consultation. Be?fore asking exception question,miracle questions should be asked in the use of solution?focused intervention.

Objective To evaluate the methods for improving diagnostic ratio of unruptured ectopic pregnancy with ultrasonography. Methods A total of 139 patients of small adnexal cysts with thick wall were predicted respectively with sonographic indexes. All patients (61 with unruptured ectopic pregnancy and 78 with small corpusluteum) were confirmed postoperatively or with long-term follow up, and the results were compared. Results The diagnostic sensitivity of with hyperecho or isoecho compared with endometrium, hyperecho compared with ovarian tissue, adnexal cyst without small follicle around was 86.89%, 93.44% and 96.72%, and the specificity of three predictors was 93.59%, 85.90% and 70.51%, respectively. Two sonographic indexes together had a sensitivity of 98.36% and a specificity of 94.87% for diagnosing ectopic pregnancy. Conclusion Observing the relation of adnexal cyst and ovarian and the echogenic of cyst wall are specific and sensitive for the diagnosis of ectopic pregnancy.

Assessing the stage of live fibrosis and cirrhosis has direct clinical implications for patient treatment options.Liver biopsy is typically considered as the gold standard, but it has limited clinical utility due to its invasiveness.In recent years, with the rapid development of CT hardware and software, CT has made important progress in the non-invasive evaluation of liver fibrosis and cir-rhosis at home and abroad.This review focuses on clinical application of of contrast-enhanced CT, CT perfusion imaging, dual-energy CT and CT molecular imaging for liver fibrosis and cirrhosis.

Objective To explore the combined detection of serum osteopontin(OPN),gastric cancer associ-ated antigen(MG7 -Ag)and tissue polypeptide specific antigen(TPS)for the clinical value of diagnosis of gastric cancer.Methods Enzyme linked immunosorbent test(ELISA)was used to detect serum levels of OPN,MG7 -Ag and TPS of 60 cases of gastric cancer patients,gastric benign lesion group 20 cases were randomly selected at the same time,the group of 24 cases of precancerous lesions and 40 healthy people were also selected as control study.Results Gastric cancer group′s serum levels of OPN,MG7 -Ag and TPS were significantly higher than those of gastric benign lesions,the precancerous lesion group and healthy control group(F =50.993,62.50,41.396,all P <0.01 ).The single detection sensitivity were 71.7%,80.0% and 67.3% respectively,three joint detection sensitivity was 91.7%.Conclusion Combined detection of serum OPN,MG7 -Ag and TPS can improve the sensitivity of diagnosis of gastric cancer,and has higher diagnosis significance in the diagnosis of gastric cancer.

Objective:To evaluate MRI manifesattions of intradural metastases of medulloblastomas.Methods:The clinical data and MRI of 6 cases with intradural metastasis of medulloblastomas were reviewed retrospectively.Results:21 masses were found in 6 cases,mostly in lumbar segment.Most of them were oblong or fusiform shagped.The size averaged 1.8 cm?2.3 cm.They had the similar signal intensity with those in brain,but hemorrhage,cyst and calcification were less common.Conclusion:MRI plays an importment role in diagnosis of intradural metastases of medulloblastomas. [

AIM: Using optical coherence tomography angiography (OCTA) to observe the changes and clinical significance of macular blood flow density in patients with diabetic retinopathy (DR).METHODS: Totally 47 eyes (28 patients) with diabetic retinopathy (DR) were enrolled in the DR group.According to the international clinical grading criteria of diabetic retinopathy, 30 eyes (19 patients) with non-proliferative diabetic retinopathy were classified as the NPDR group, and 17 eyes (11 patients) with proliferative diabetic retinopathy were classified as PDR group.A total of 46 (27 subjects) healthy eyes with matched age were enrolled in the control group.All the subjects underwent the 3mm×3mm scanning of macular retina by optical coherence tomography angiography (OCTA), obtaining 4 levels of macular blood flow density map.The macular blood flow density at 3 levels, including superficial retinal layer, deep retinal layer and choroidal capillaries layer, were measured.RESULTS: The macular blood flow density of superfical retinal layer, deep retinal layer and choroidal capillaries layer in DR group were 0.4963±0.0840, 0.4798±0.0801 and 0.5290±0.0528, respectively.Among them, the blood flow density of each layer were 0.5064±0.0843,0.4983±0.0766,0.5345±0.0529, respectively, for the NPDR group, and were 0.4786±0.0830, 0.4473±0.0778,0.5192±0.0526, respectively, for the PDR group.For the control group, the density of each layers were 0.5919±0.0704, 0.6301±0.0527, 0.5691±0.0169, respectively.The macular blood flow density was significantly different in the superficial retinal layer, deep retinal layer and choroidal capillary layer between the control group and the NPDR group, as well as the PDR group and the DR group (total P<0.001).Statistically significant difference was found between the NPDR group and the PDR group in the deep retina layer (P=0.029), but not in the superficial retina layer and choroid capillary layer (P=0.236, 0.268).CONCLUSION: Compared with the control group, the macular blood flow density of superficial retinal layer, deep retinal layer and choroidal capillary layer in the patients with diabetic retinopathy decreased significantly.It indicated that the macular ischemia existed in both retina and choroid.By quantitatively measurement of the macular blood flow, OCTA may be used for monitoring the progression of diabetes, and early detection of diabetic retinopathy.

Objective To establish a cell level-based negative enrichmen technique to detect circulating tumor cells (CTCs) in the peripheral blood of patients with epithelial ovarian cancer.Methods The colon cancer SKOV-3 cells were mixed with 2 ml whole blood from healthy donors at different ratio.Quantification of CTCs was performed using immunomagnetic bead based negative enrichment combined with immunofluorescence antibody method.The method was evaluated the recovery rate of target cells,Samples of 32 patients with ovarian cancer and 10 controls were assayed for CTCs detection by above method.Results ①The recovery rate was ranging from 64％ ～80％ by spiking varying numbers of SKOV-3 into 2 ml blood samples of healthy volunteers.Regression analysis of number of recovered SKOV-3 cells yielded a regression equation of Y=0.782X-1.408 and a coefficient of determination of R2 =0.998.②Did not detect CK8/18-+ circulating tumor cells in 10 controls,and CK8/18+ circulating tumor cells in 18 cases of 32 Patients with ovarian cancer.The positive rate of CK 8/18 + circulating tumor cells was significantly differences between the two groups (x2 =7.681,P＜0.01).③The presence ofCTCs was significantly correlated with distant metastasis (x2 =5.776,P＜0.05).Conclusion The method of immunomagnetic bead based negative enrichment combined with immunofluorescence antibody technique for CTCs detection in peripheral blood of patients with ovarian cancer has a clinical value of application and extension.

Adenocarcinoma ; classification ; pathology ; surgery ; therapy ; Cardia ; Chemotherapy, Adjuvant ; Esophageal Neoplasms ; classification ; pathology ; surgery ; therapy ; Esophagectomy ; methods ; Esophagogastric Junction ; surgery ; Gastrectomy ; methods ; Humans ; Neoplasm Staging ; Radiotherapy, Adjuvant ; Stomach Neoplasms ; classification ; pathology ; surgery ; therapy