Objective To investigate the clinical efficacy of iron polysaccharide complex in the treatment of iron deficiency anemia and its effect on content of serum transferrin receptor(sTfR)and serum ferrin(SF).Methods 132 patients with iron deficiency anemia in Guizhou Provincial People’s Hospital were received iron polysaccharide complex for one month,we analysed the clinical efficacy of iron polysaccharide complex in the treatment of iron deficiency anemia.The content of blood indicators,serum iron(SI),sTfR and SF were detected and analyzed before and after treatment. Results Iron polysaccharide complex had a good effect on iron deficiency anemia,the obvious effective rate was 59.09%and total efficiency was 85.61%;blood indicators and SI improved significantly(P<0.05),sTfR decreased significantly(P<0.05),SF increased significantly(P<0.05);SI and sTfR,SF, sTfR/SF has a good linear relationship relatively.Conclusion The mechanism of iron polysaccharide complex on patients with iron deficiency anemia is related with SF levels increased and sTfR levels reduced.

[Objective]To explore the feasibility of SF-36 in assessment of health-related quality of life(HRQOL) in patients with total hip replacement.[Method]The reliability,validity and responsiveness of the SF-36 in 90 total hip replacement patients were analyzed.[Result]The SF-36 was of better validity by factor analysis.All domains of the scale,the test-retest reliability correlation coefficients were better and internal consistency coefficients were more than 0.7 for five domains,except role-physical 0.43;vitality 0.57;role emotional 0.15.The SF-36 could detect the change of quality of life over before and after treatment sensitively(P

Objective: To analyze effect of ulinastatin on patients’ inflammatory factors level during cardiopulmonary bypass valve replacement surgery. Methods: Chosen patients accept cardiopulmonary bypass valve replacement surgery between 2011 to 2013 as research subjects, randomized to receive Ulinastatin as observation group and volume of saline as control group, testing inflammatory levels and pulmonary ventilation function difference. Results: One hour after CPB and 1h after the end of CPB, TNF-α, IL-6 level as well as A-aDO2 level of observation group were lower than control group; (2)A-aDO2 levels were positively correlated with TNF-α, IL-6 levels. Conclusion: Ulinastatin can effectively reduce cardiopulmonary bypass valve replacement surgery preoperative inflammatory cytokine levels, reducing the impact on pulmonary ventilation function, inflammatory levels and pulmonary ventilation function was negatively correlated.

Pancreatic cancer (PC)is one of common malignant digestive diseases.It is mostly diagnosed at advanced stage,with an ex-tremely poor progression.The relationship between diabetes and PC was shown by numerous epidemiological studies for decades.Retrospec-tive clinical studies and research on molecular mechanisms in recent years have led to a new understanding of the relationship between diabe-tes,especially new-onset diabetes,and PC,the effect of antidiabetic medication on PC,and the molecular mechanisms underlying the con-nection between diabetes and PC.It is suggested by recent data that long-standing diabetes is one of the risk factors for PC development, new-onset diabetes may facilitate early diagnosis of PC,diabetes may have an impact on the prognosis of PC,the option of antidiabetic medication may influence the incidence of PC,and exploring the molecular mechanisms underlying the association between diabetes and PC may help to identify the new therapeutic target for PC.

The ophthalmological teaching of clinical laboratory and preventive medicine specialty should base on their own characteristics,and the teaching reform must be conducted form the four aspects of material,outline,practice and test to adapt to the demands of society development.

Objective To explore the effect of carnosic acid combined with tamoxifen on breast cancer cells and the mechanism.Methods Breast cancer cell lines MCF-7,T47D were divided into 4 groups:PBS was given to control group,carnosic acid(CA)was added to CA group,tamoxifen(TAM)was added to TAM group,and CA+TAM group was treated with carnosic acid and tamoxifen.The proliferation ability was measured by MTT assay,the colony formation capacity was measured by colony formation assay,the invasion ability was measured by Transwell chamber assay,the migration ability was measured by wound-healing assay,the expression of Caspase-8,Caspase-3 and PARP protein were measured by Western blotting.Results CA,TAM and CA+TAM suppressed breast cancer cell proliferation with a dose-dependent manner.Compared with CA group and TAM group,the proliferation inhibitory rate of CA+TAM group was significantly increased(P<0.05).In MCF-7 and T47D cell lines,compared to control group,the rate of colony fromation,number of invasive cells and cell migration rate of CA,TAM,CA+TAM groups were significantly decreased(all P<0.05).These indexes mentioned above in CA+TAM group were significantly lower than those in CA group and TAM group(all P<0.05),but there were no significant differences between CA group and TAM group (all P>0.05).In two breast cancer cell strains,the expression of cleaved Caspase-8 protein between control,CA,TAM and CA+TAM groups was not significantly different(P>0.05).Compared to control group,the expression levels of cleaved caspase-3,cleaved PARP protein in CA,TAM and CA+TAM groups were significantly increased(P<0.05),and these protein levels were significantly higher in CA+TAM group than in CA and TAM group(all P<0.05).But there were no significant differences between CA group and TAM group(all P>0.05).Conclusion Carnosic acid combined with tamoxifen can suppress the proliferation,invasion and migration of breast cancer cells,and its mechanism may be related to the activation of Caspase-3/PARP signaling pathway.

Metabolism of n-alkane mainly includes ?-oxidation pathway and ?-oxidation pathway in Candida tropicalis,which are both catalyzed by a series of enzymes. The transformation from n-alkane to fatty acid is accomplished via ?-oxidation pathway,and the degradation of fatty acid is involved in ?-oxidation pathway. The mechanism on metabolism of n-alkane and studies on related enzymes are summarized in the review. Furthermore,some molecular genetics and metabolic engineering techniques related to Candida tropicalis developed in recent years are introduced.

Objective To investigate and analyze the behavior and therapeutic status of rheumatoid arthritis (RA) patients. Methods Out patients diagnosed with rheumatoid arthritis in our hospital from May to August 2007 were enrolled. The data including sex, age of onset, site of first hospitalization and medication status were collected and analyzed. Results In this 181 RA patients, the mean age of onset was (53±11) year-old, mean history duration was (10±8) years, the ratio of male to female was 1:4.2. The orthopedics department was the most common site of first hospital visit (32.0%, 58/181) and rheumatology department was the most common site to clarify the diagnosis (62.4% ,113/181). The diagnosis delay caused by patients themselves was (5.9±17.2) months and the delay caused by doctors was (9.0±22.0) months. More than half of the patients were not treated appropriately before they came to our hospital. Methotrexate was the most commonly used DMARDs (67.3%), followed by leflunomide (46.4%), sulfasalazine (37.5%) and hydroxyehloroquine (19.6%). Conclusion In this cohort, the proportion of patients who come to rheumatology department immediately after disease onset is low. There is delay between symptoms and final diagnosis. More than half of the patients are not treated appropriately.

Objective To discuss the value of hypotonic-MRCP combined with LAVA dynamic contrast-enhanced scan in diagnosis of duodenal tumor.Methods Five patients with duodenal adenocarcinomas confirmed by pathology were involved in this study.All cases underwent plain MRI and hypotonic-MRCP and LAVA dynamic contrast-enhanced scans.Results 4 of 5 cases were correctly diagnosed with MR imaging.Imaging signs included:the cavum of descending duodenum appeared as asymmetrical stenosis,masses with soft tissue signal intensity connecting the intestinal wall with wide base,the intestinal wall stiff,the intestinal mucosa destruction.The lesions would be slightly-moderately enhanced at LAVA dynamic enhancement scanning.Conclusion Hypotonic-MRCP combined with LAVA dynamic contrast-enhanced scan can display the direct and indirect sign of duodenal tumors,which was effective in identifying the circumscription of the tumor and its extension.

Objective:To explore the application value of transesophageal echocardiography (TEE)in secondum atri-al septal defect (ASD)intervention and minimally invasive occlusion surgery in adult patients.Methods:A total of 134 cases adult patients (>15 years)with pure secondum ASD (including 91 cases undergoing interventional occlu-sion and 43 cases undergoing minimally invasive occlusion surgery),who were screened via transthoracic echocardio-graphy (TTE)and TEE from Jan 2012 to Dec 2012,were selected.Intervention group and minimally invasive sur-gery group received TTE and TEE monitoring respectively during operation.Preoperative TTE and TEE parameters and operation results were compared and analyzed.Results:The parameters of ASD diameter measured by TEE were significantly higher than those measured by TTE in two groups (P <0.01 all).Maximum defect diameter of ASD measured by TEE was significantly higher than that of measured by TTE [(19.8±5.2)mm vs.(18.7±4.9)mm], P <0.01. The correlation between maximum defect diameter measured by TTE and occluder size (intervention group r =0.926,minimally invasive surgery group r =0.215)was all lower than that of TEE (intervention group r=0.965,minimally invasive surgery group r =0.627),P <0.01 both.Conclusion:TEE is better than TTE in as-sessment of ASD size.It should be regarded as a routine preoperative screening method for adult ASD patients.It has important value in real-time monitoring,guidance and immediate evaluation of therapeutic effects during mini-mally invasive ASD occlusion surgery.