Patients replanted severed finger generally have negative emotion after operation. Negative mood could induce vascular crisis and reduce survival of replanting finger. This paper reviewed the studies on negative emotion and psychological intervention in patients after digit replantation in various stages post-operation, and prospected the future.

BACKGROUND: The rare earth elements (Res) have multiple bio-activities and some extent neurotoxicity, Because of their distinct physical and chemical properties. The studies on neuromuscular junction and sympathet ic ganglia have shown that some Res, such as lanthanum(La), gadolinium (Gd),etc, exert considerable effects on synaptic transmission, but the effects and mechanism of Samarium on synaptic transmission are still unknown.OBJECTIVE: To investigate the effects and impossible mechanism of Samarium Chloride (SmCl3) on the nicotinic transmission in the isolated sympathetic ganglia, superior cervical ganglion (SCG) of rats.DESIGN: Controlled experimental study based on cells.SETTING: Department of Pharmacology, Guangxi Medical University. MATERIALS: Totally 40 adult Wistar rats (weighing 250-300 g) of either sex, provided by the Experimental Animal Center of Guangxi Medical University, were used in this study. SmCl3 was made by the chlorination of Samarium Oxide with purity 99.5% and relative molecule mass 348.7, presented by Professor Liu Da-yuan, Guangxi Medical University. Acetylcholine chloride (Ach) and carbachol (Carb) were purchased from Sigma.METHODS: The experiment was completed at the neuropharmacology lab of the experimental center of Guangxi Medical University from September 2001 to December 2002. After sacrificing animals by acute exsanguination,SCG together with their preganglionic nerve trunks were isolated rapidly,then transferred to the recording chamber, the preganglionic nerve trunk was drawn into a suction electrode for orthodromic stimulation. The ganglia were superfused continuously with a Krebs solution, saturated with 950 mL/L 02 and 5mL/L CO2, pH 7.4±0.05, (34±0.5) ℃.The fiber containing glass microelectrodes filled with 3 mol/L KC1 (30-60 MΩ tip resistance) were used to impaled cells and do intracellular recording. The fast excitatory postsynaptic potentials (FEPSPs) were evoked in SCG neurons by single pulse stimulations (0.2-0.5 Hz, 0.5-1.0 ms, 2-10 V)on preganglionic nerve trunk. The remarkable membrane depolarization would be recorded in SCG neurons by superfusing ganglia with exogenous Ach (0.1 mmol/L) or Carb(0.1 mmol/L) for 30-60 s. The effects of 1×(10-7-10-4) mol/L SmCl3 on FEPSPs, membrane potentials, membrane resistance, exogenous Ach and Carb-induced membrane depolarization of SCG neurons were investigated in this experiment.The effects of SmCl3 on the facilitation of high Ca2+ (10 mmol/L ) on FEPSPs were also be observed, namely, first superfusing the ganglia with high Ca2+ (10 mmol/L)to facilitate FEPSPs, then superfusing the ganglia with Ca2+(10 mmol/L)contained SmCl3. All the drugs were solved in Krebs solution or improved Krebs solution and applied to ganglia by superfusion in known concentration.The bioelectricity difference before and after the drug superfusion were analyzed by paired Student's t test.MAIN OUTCOME MESURES: ①Effects of SmCl3 on FEPSPs.②Effects of SmCl3 on membrane potentials and membrane resistances. ③Effects of SmCl3 on exogenous Ach and Carb-induced membrane depolarization. ④Effects of SmCl3 on the facilitation of high Ca2+ (10 mmol/L ) on FEPSPs.RESULTS: ① 1 ×(10-7-10-4)mol/L SmCl3 could reversibly depressed the FEPSPs of rats SCG neurons [the amplitude inhibitory percentage of FEPSPs of l×10-4, 1×10-5, 1×10-6, 1×107 mol/L SmCl3 was (49.78±13.85)%(n=20),(39.05±4.05)%(n=10),(29.83±9.73)%(n=10)and (16.30±2.16)%(n=10)respectively (P ＜ 0.05-0.01)].1×10-4 mol/L SmCl3 could chang Aps into FEPSPs (n=5).②The membrane depolarization induced by Ach (n=5) and Carb (n=7) were not significantly changed by 1×10-4 mol/L SmCl3(P ＞ 0.05).③The membrane potential and membrane resistance were not significantly altered by 1×(10-7-10-4)mol/L SmCl3(n=67), P ＞ 0.05. ④1×10-4 mol/L SmCl3 could antagonized the facilitation of high Ca2+ (10 mmol/L ) on FEPSPs (n=5), P ＜ 0.01.CONCLUSION: SmCl3 can depresses nicotinic transmission in rats sympathetic ganglia by presynaptic mechanisms, perhaps due to its inhibition on Ca2+ influx.

BACKGROUND:To control blood glucose, blood pressure, blood lipids and inhibit the rennin-angiotensin system is the main idea focused on the treatment of diabetic nephropathy, but the curative effect is unsatisfactory. Hemodialysis and kidney transplantation are suitable for serious cases, however, which is restricted because of the limited source of kidneys and high cost. Regenerative medicine research based on stem cells brings a new hope for treatment of diabetic nephropathy. OBJECTIVE:To comprehensively analyze the mechanism underlying different sources of stem cells for treatment of diabetic nephropathy and the clinical implications. METHODS:Papers addressing stem cells for the treatment of diabetic nephropathy were retrieved by computer in CNKI database and PubMed database from January 2005 to August 2013 with the key words“embryonic stem cells, bone marrow mesenchymal stem cells, induced pluripotent stem cells, diabetic nephropathyin Chinese and English. Papers published recently or in journals with high impact factor were selected. A total of 60 papers were included for review. RESULTS AND CONCLUSION:Embryonic stem cells, bone marrow mesenchymal stem cells, induced pluripotent stem cells have the potential to differentiate into renal histiocytes. A large numbers of experimental studies have shown that stem cells transplantation has a positive effect on recovery of injured kidney. Stem celltransplantation can provide a novel therapy for diabetic nephropathy.

Objective To study the relationship among hydrops fetalis(over 28 gestational weeks),Mirror syndrome and preeclampsia(PE). Methods Clinical data of 13 cases of hydrops fetalis were analyzed and relative publications were reviewed. Results Eight out of the 13 cases were diagnosed as PE,and 5 were Mirror syndrome.Serious maternal complications occurred in all cases with either Mirror syndrome or PE. ConclusionsFetaland placental hydrops was closely related with maternal symptoms.Clinicians should be aware of this condition and detect Mirror syndrome in time so as to prevent its progress to PE.However,the pathogenic mechanism remains to be fully elucidated.More attention should be paid to both mothers and the hydrops fetalis.

OBJECTIVE:To investigate the status quo of the use of Chinese patent medicines in our hospital and to put forward the coping strategies.METHODS:The data about the consumption of Chinese patent medicines in our hospital during the period of 2002～2006 obtained from medical information platform were analyzed in respect of consumption sum,DDDs,and DDC using the method of DDDs sequence and the method in pharmacoeconomics.RESULTS:The use of Chinese patent medicines in our hospital during 2002～2006 took on an obvious rising trend.CONCLUSION:The utilization of Chinese patent medicines in our hospital is rational basically.The Chinese patent medicines with suitable prices and proved efficacy take a great share in the clinical application.However,there are still some problems remain to be standardized.

OBJECTIVE: To investigate the utilization and development trend of heat-clearing drugs(HCDs) in our hospital so as to provide references for rational drug use and reasonable drug purchase.METHODS: The consumption quantity and consumption sum of HCDs during the period 2006～2007 were investigated,and the DDDs and daily consumption sum of HCDs were analyzed using DDDs sequence analytical method.RESULTS: The consumption quantity of HCDs in the hospital during 2006～2007 showed a steady increase and the DDDs of HCDs increased synchronously.CONCLUSION: The utilization status of HCDs in the hospital is basically in consistent with national drug consumption tendency.

Objective To study the relationship between carotid intima media thicknes(IMT) and cerebral infarction in patients with type 2 diabetes.Methods The IMT of the carotid artery was measured by high resolution B mode ultrasound, the relationships between carotid IMT and risk factors of cerebrovascular disease were evaluated.Results The serum levels of fibrinogen, total cholesterol and low density lipoprotein cholesterol were significantly higher in diabetic subjects with cerebral infarction than those in control group ( P

AIM: To observe the functional recovery of knee joint in patients with posterior cruciate ligament (PCL) under knee arthroscopy with ligament advanced reinforcement system (LARS) artificial ligament transplantation. METHODS: From January 2005 to May 2006, six patients with PCL injury treated by reconstruction of PCL with LARS artificial ligament transplantation were selected from the Department of Orthopedics, Huadu Hospital Affiliated to Southern Medical University. All subjects agreed with the operation before the surgery and all reconstruction surgeries were of second period. ① The tibial tunnel was established firstly. ② Then was the femoral tunnel. ③ Under knee arthroscopy, the "Y"-shape double cords of LARS artificial ligament were cut through ante-out and meta-in bone tunnels of femur, and fixed at 90o and 30o flexion respectively. Patients were required to motor 3 days postoperative, and patients actively exercised their quadriceps muscle of thigh at one week later. Regular follow-ups were conducted postoperatively, and functional evaluation of the knee joints was performed by according to Lysholm standards, including 8 aspects: Limping, supporting, interlocking, unstable, painful, swell, upstairs and downstairs and squatting with a total score of 100 points. The higher the score was, the better the functional recovery of knee joint. RESULTS: All of the 6 patients were available at follow-up, and no one withdrew from the study. The follow-up was conducted at the 7th, 7th, 10th, 10.4th, 12.8th, and 14th months with an average duration of 10.2 months. After operation, unstable symptoms of knee joint in all subjects disappeared with negative sag sign and posterior drawer. The Lysholm knee scores of patients postoperation were significantly higher than those before the surgery (83.5?4.5, 51.6?3.6, P

Clinical communication skills are the one of seven lowest requirements of global medical education.This review emphasizes the importance of cultivating communication skills in general medical,indicates the defections of curriculum installation and discusses the training mode and examination mechanism.

In this paper, the importance of using advanced medical equipment to check treatment was ana-lyzed, and then pointed out that nowadays there were the following problems: the medical staff had excessive de-pendence on medical equipment; medical equipment inspection treatment of irrationality; medical equipment in-spection treatment with humanity was not enough; medical equipment management of dereliction of duty. Put for-ward to strengthen medical personnel professional moral education;to strengthen the medical students' ethical edu-cation;regular hospital manage concept;improve the cognitive level of medical staff of medical equipment.