OBJECTIVE:To investigate the effects of application timing of carboporst tromethamine on postpartum hemor-rhage and hemodynamics of cesarean section women with centralplacenta previa. METHODS:Totally 138 parturient women with central placenta previa selected from our hospital during Jan.-Dec. 2015 were divided into observation group and control group ac-cording to random number table,with 69 cases in each group. Both groups received cesarean section. Control group was given Car-boporst tromethamine injection 250 μg on uterine body after the delivery of placenta. Observation group was given Carboporst tro-methamine injection 250 μg on corpora uteri immediately after umbilical cord cutting. The operation duration,intraoperative and postoperative blood loss were observed in 2 groups. The preoperative and postoperative 24 h hemodynamic index,coagulation func-tion and corpora uteri were compared between 2 groups. RESULTS:The operation duration of observation group was significantly shorter than control group;intraoperative blood loss and 24h blood loss of observation group were significantly less than control group,with statistical significance(P<0.05). There was no statistical significance in preoperative hemodynamic index and coagula-tion function index between 2 groups before surgery(P>0.05). 24 h after surgery,SBP,DBP,MAP,FIB and D-D of 2 groups were decreased significantly,while HR of them were increased significantly;the observation group was significantly better than the control group,with statistical significance (P<0.05). There was no statistical significance in the incidence of ADR (15.94% vs. 13.04%) between 2 groups (P>0.05). CONCLUSIONS:Carboporst tromethamine after omphalotomy via uterine body helps to shorten the operation time,reduce intraoperative blood loss,maintain hemodynamic indexes stable and improve coagulation func-tion of cesarean section women with central placenta previa ,with good safety.

To observe the effect of aerobic exercise on the body shape,cardiovascular,physiology and biochemical indexes of elderly women,we trained 76 old women (volunteers,mean age 58 years old) from urban area of Yulin from 14th July 2005 to 14th January 2006,60 minutes every time,3 times a week. The heart rate during exercise was controlled within 110-125 times per minute. The changes in their body shape (body mass and waistline),cardiovascular function (resting heart rate and blood pressure) and biochemical indexes before and after exercise were observed. The results showed that after 24 weeks of aerobic exercise,the body mass and waistline of the subjects were decreased compared with that before exercise (P

[Objective] To observe the proliferation of T and B Lymphocyte in mouse after treated by Hirsutella sinensis strain fermentation.[Methods] Mice were treated with 1.0g?kg-1,0.1g?kg-1 of Hirsutella sinensis strain fermentation by intragastric administration.The proliferation of T and B Lymphocyte from the spleen of mouse is detected by the MTT assay .[Results] Hirsutella sinensis strain fermentation and Cordyceps Sinensis can distinctly increasse the stimulation index (SI) of T and B lymphocyte transformation.[Conclusion] Hirsutella sinensis strain fermentation and Cordyceps Sinensis are able to enhance immune function of normal mouse.

Objective To approach work pressure and its influencing factors in general surgical nurses. Methods 105 general surgical nurses were selected from 8 hospitals with stratification clus-tering randomization methods. The job stressor inventory,- personality scale, coping styles questionnaire and general state of health questionnaire were used to carry out investigation and the results underwent analysis. Results The main stressors came from the factor of workload and time allocation, then from nursing specialty and job. Working time more than 10 years, undergraduate degree or above, work shift, neuroticism, wrapping, avoidance, letting off were risk factors of work pressure in general surgical nurses. Age more than 40 years old, nurse and associate director, exotrophy, planning to solve the ques-tions, seeking social support, rational coping were protective factors of work pressure for nurses. Con-clusions The overall pressure level of general surgical nurses was moderate. We should cultivate favourable personality of nurses and positive coping styles in combination with their demography char-acters to prevent work pressure.

Purpose:To clarify roles of overexpression of phosphorylated Akt(p-Akt) and loss of phosphatase and tensin homologue deleted on chromosome 10(PTEN) expression in biological behavior of non-small-cell lung cancer(NSCLC). Methods:Immunohistochemical staining was used to determine the expression of p-Akt and PTEN in 20 cases of normal lung tissues and 102 patients with NSCLC.Results:Negative p-Akt expression and positive PTEN expression were found in normal lung tissues, while the positive incidences of p-Akt expresssion and loss of incidences of PTEN expresssion in NSCLC were 41.2% (42/102) and 46.1% (47/102)respectivtly.The overexpression of p-Akt and loss of PTEN expression were correlated to degree of differention of cancer, metastasis(including lymph node), and clinical stages. A significant negative correlation was observed between expression of p-Akt and PTEN(Phi r=-0.425,P

Mucus secreted mainly by epithelial goblet cells and submucosal glands covering the respiratory tract plays an important role in the protection from external aggressions, such as solid particles, pathogens and chemical agents by mucociliary clearance. The viscoelastic properties of mucus are mainly determined by the presence of extensively-glycosylated high molecular weight mucins. A lot of factors influence the expression and secretion of mucins in airway, lead to mucus overproduction, which is a distinguishing feature of chronic obstructive pulmonary disease (COPD) and causes disruption of the mucociliary clearance function, resulting in airway block, chronic infection and death.

0.05)respectively.[Conclusion]The technique of radiographic assessment to define the anteromedial OA of the knee is not easy to perform,but it can be used,by well trained doctors,in the most knee OA cases with high accuracy.The MR imaging gives more information about cartilage defects and can be used in some doubted cases.

Objective To study the role of polyamines(putrescine,spermidine and spermine) in regulating ornithine decarboxylase(ODC) protein levels in the early regenerating liver of rats. Methods Exogenous polyamines(dissolved in 0.9% NaCl) were administered subcutaneously to adult male SD rats(180～220g).The ODC protein levels in the regenerating liver induced by partial hepatectomy(PH) were measured by Western blotting analysis. Results The treatment with high doses of putrescine( 20 mg/kg body weight),spermidine(0.15 mg/kg body weight) or spermine(6 mg/kg body weight) reduced the ODC protein level compared with the control group within 12 hours after PH.However,the treatment with low doses of putrescine(0.02 mg/kg),spermidine(0.03 mg/kg) or spermine(0.06 mg/kg) resulted in a rapid increase in the protein level in the 4th hour after PH,which was 15.1%,29.5% or 30.3% higher than that of control rats respectively.Conclusion Polyamines with certain doses play a role in the feedback regulation of ODC protein levels in the early regenerating liver,where spermidine and spermine play a greater role than putrescine dose.

Objective To study the effect of preoperative immunonutrition on nutritional status and immunity for the patients with gastrointestinal cancer, and to evaluate whether the administration of immunonutrition could reduce the rate of postoperative complication and thus shorten the days of postoperative hospitalization. Methods Sixty patients with different gastrointestinal cancer were randomized to receive either immunonutrition (containing Arginine, RNA and ?-3 polyunsaturated fatty acids) for 7 days before surgery (EN, n=30) or a conventional diet (CONT, n=30). Variables of nutritional status and immunity, postoperative complications, infection and the days of postoperative hospitalization were measured. Results There were no significant differences in the immunological and nutritional variables between two groups before surgery. The serum concentrations of prealbumin(PALB) and transferrin(TRF) were lower than those before surgery in the CONT group on postoperative day 3 (P

BACKGROUND:Mechanical signal has close correlation with the growth, development, repair and reconstruction of the skeletal system and the development of disease, the effect and the mechanism on bone marrow mesenchymal stem cel s is worthy to concern.
OBJECTIVE:To explore the effect and mechanism of hydrostatic pressures on the differentiation of bone marrow mesenchymal stem cel s.
METHODS:Short-term experiment:the human bone marrow mesenchymal stem cel s were incubated into the normal Dulbecco’s modified Eagle’s medium, osteogenic medium or the Dulbecco’s modified Eagle’s medium containing extracel ular signal-regulated kinase 1/2 inhibitor U0126, respectively. Homemade pressure loading system was used to impose 0, 40 and 80 kPa hydrostatic pressure for 1 and 4 hours. Long-term experiment:human bone marrow mesenchymal stem cel s were incubated into the normal Dulbecco’s modified Eagle’s medium or osteogenic medium respectively, and then 40 kPa hydrostatic pressures was loaded for 4 hours per day, and lasted for 14 days. The cel s without hydrostatic pressure were regarded as the control group.
RESULTS AND CONCLUSION:Real-time quantitative reverse transcription PCR results showed that after osteogenic induction and simulated with 40 kPa hydrostatic pressure for 4 hours, the mRNA expressions of core binding factorα1 and osteocalcin in the bone marrow mesenchymal stem cel s were increased, while the mRNA expressions of peroxisome proliferator-activated receptorγ2 and adipsin were decreased, and the 80 kPa hydrostatic pressure did not cause such reactivity. The osteogenic induction effect of 40 kPa hydrostatic pressure could be partial antagonized with U0126. Histochemical staining showed that after simulated with 40 kPa hydrostatic pressure for 7 days, the expression and activity of alkaline phosphatase of bone marrow mesenchymal stem cel s were increased;after lasted for 14 days, the mRNA expressions of peroxisome proliferator-activated receptorγ2 and adipsin were increased. Certain intensity and duration of hydrostatic pressure stimulation can regulate the differentiation of bone marrow mesenchymal stem cel s, and the mechanism is only partly mediated by the extracel ular signal-regulated kinase 1/2 signaling pathway.