Electrocardiography ; Humans ; Hypertension ; diagnosis ; physiopathology ; Hypertrophy, Left Ventricular ; diagnosis ; physiopathology

Anthracyclines ; pharmacology ; therapeutic use ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Breast Neoplasms ; drug therapy ; metabolism ; Cisplatin ; administration & dosage ; Deoxycytidine ; administration & dosage ; analogs & derivatives ; Drug Resistance, Neoplasm ; Female ; Humans ; Taxoids ; administration & dosage ; Vinblastine ; administration & dosage ; analogs & derivatives

Antineoplastic Agents, Hormonal ; therapeutic use ; Antineoplastic Agents, Phytogenic ; therapeutic use ; Breast Neoplasms ; drug therapy ; prevention & control ; secondary ; surgery ; Chemotherapy, Adjuvant ; methods ; Early Detection of Cancer ; methods ; Female ; Humans ; Tamoxifen ; therapeutic use ; Taxoids ; therapeutic use

Breast Neoplasms ; diagnosis ; therapy ; Chemotherapy, Adjuvant ; Combined Modality Therapy ; Female ; Humans ; Neoadjuvant Therapy

Alzheimer's disease (AD) is a most common neurodegenerative disease. The mechanisms underlying AD, especially late-onset AD, remain elusive. In the past few years, results from genome-wide association studies (GWAS) and systems approaches indicated that innate immune responses mediated by microglia played critical roles in AD. Functional analysis on animal models also showed that immune receptors or proteins expressed in microglia mediated Abeta-induced inflammation, or Abeta phagocytosis by microglia. Microglia plays double sword roles in AD. More work is warranted to elucidate the exact roles of microglia in AD, which will facilitate our better understanding of the mechanisms underlying AD.
Alzheimer Disease ; pathology ; Animals ; Disease Models, Animal ; Genome-Wide Association Study ; Humans ; Inflammation ; pathology ; Microglia ; physiology ; Phagocytosis ; Receptors, Immunologic ; physiology

Aged ; Aged, 80 and over ; Breast Neoplasms ; epidemiology ; pathology ; physiopathology ; therapy ; China ; epidemiology ; Female ; Humans ; Middle Aged ; Treatment Outcome

Objective: To establish a method for simultaneous determination of the contents of six main active constituents (neochlorogenic acid, chlorogenic acid, rutin, hyperin, isoquercitrin, and quercitrin) in Houttuyniae Herba by quantitative analysis of multi-components by single marker (QAMS). Methods: The chromatographic separation was achieved on a Kromasil C18 column (250 mm × 4.6 mm, 5 μm), the mobile phase was acetonitrile-0.1% phosphoric acid aqueous with gradient elution and changeable wavelength detection. Chlorogenic acid was used as the reference, the calibration factor of other five constituents to that of chlorogenic acid was calculated respectively to get each relative calibration factor. And the contents of the active constituents were calculated by the relative calibration factor to realize QAMS. At the same time, the external standard method was used to detect the contents of the six constituents, comparing the two methods to validate the accuracy and feasibility of the new method. Results: No significant differences were found in the quantitative results of the six active constituents in Houttuyniae Herba determined by measured value and calculated value. The amounts of the six active constituents in the different parts of Houttuyniae Herba were found to be varied, chlorogenic acids and flavonoids mainly distributed in the leaves. Conclusion: The active constituents of Houttuyniae Herba mainly distribute in the leaves. The QAMS is feasible and accurate to evaluate the contents of the six active constituents in Houttuyniae Herba.

Objective: To establish a new method for quantitative analysis on multi-components by single marker (QAMS) and validate its feasibility and technical adaptability in analysis on Shuangqing Yanhou Tablets (SYTs) for the simultaneous determination of 10 main constituents (citric acid, gallic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, ammonium glycyrrhizinate, and artemisia ketone). Methods: Using SYTs as objects and the above 10 constituents as indexes, three correction methods were used to establish the relative correction factor (fk/s) between each component and gallic acid, respectively then to calculate the amount of each component and finally to achieve QAMS. At the same time, the external reference method and regression equation method were used to determine the amounts of the above 10 constituents, to compare the difference between the calculated and real data of the three fk/s, and to validate the correctness and adaptability of QAMS. Results: No significant difference was found in the quantitative results of 10 active constituents in three batches of SYTs determined by the calculated and real data. Conclusion: The QAMS by three correction methods is feasible and accurate to evaluate the contents of the 10 constituents in SYTs.

OBJECTIVE: To establish a HPLC method for the simultaneous determination of the contents and fingerprint of 7 active constituents (neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isochlorogenic acid B, isochlorogenic acid A, and isochlorogenic acid C) in honeysuckle extract. METHODS: The chromatographic separation was achieved on a AkzoNobel Kromasil C18 column(4.6 mm × 250 mm, 5 μm), the mobile phase was acetonitrile-0.1% phosphoric acid aqueous solution with gradient elution at a flow rate of 1.0 mL · min-1, the detection wavelength was set at 326 nm, and the column temperature was 30°C. RESULTS: All the 7 compounds showed good linearity in the ranges of the test concentrations. The RSDs of the precision, stability and reproducibility tests were less than 3%. The average recoveries were in the range of 97.98%-99.29%. CONCLUSION: This method is simple, sensitive, accurate, and can be used for quality control of honeysuckle extract. Copyright 2012 by the Chinese Pharmaceutical Association.

Child ; Cystitis ; Eosinophils ; Humans ; Male