Automation of diagnostic labs are being developed at full speed in China,but some unreasonable large scale and comprehensive import are preferred.Consequently lots of valuable equipments have not been fully used and great recourses have been wasted.So the hospital and diagnostic lab policy makers should ask themselves about the equipment scale to match the requirement.

Subarachnoid hemorrhage ( SAH) especially aneurismal SAH is a deadly cerebrovascular disorder with very high mortality and morbidity rates, but there is still no effective treatment in clinical practice.In recent years, more and more studies have suggested that early brain injury ( EBI) may be the main factor for poor prognosis of SAH.The pathogenesis of EBI after SAH includes a series of complicated pathophysiological changes.This paper reviews the related progress of EBI after SAH from the aspects of acute cerebral ischemia, the disruption of blood brain barrier, apoptosis, autophagy, oxidative stress, inflammation, and so on.

Objective To design a Chinese phonetic laboratory system for clinical blood and urine analysis with Chinese pronunciation. Methods The database/table for saving data of patients is established on the basis of Microsoft Access 2000. The Windows Chinese speech function API is used for Chinese pronunciation. The codes of all objects are translated to an executive file which is set up with Visual Basic 6.0 tool kit. Results All data received from 100,000 samples are saved without error in the program running. The Chinese pronunciation is clear. Conclusion This Chinese phonetic laboratory system is effective and flexible in managing the data from blood analyzer and urine analyzer, which helps to improve the standardization of laboratory data report in Out-patient Department.

GeneXpert is an advanced molecular biological detection system developed in recent years, it integrates and automates the sample preparation , nucleic acid purification, gene amplification, results report of the fluorescent quantitative PCR process.As, an accurate, rapid, biosafe technology , GeneXpert has become one of the novel molecular POCT detection technology platforms for diagnosis of infectious pathogens and especially declared it a major milestone for global TB diagnosis .This paper introduces the detection principle , clinical application in the detection of infectious pathogens , limitation and prospect of GeneXpert.

In present,clinical laboratory is required to continuously improve the quality management system for providing almost perfect reports and services.The new conception and methodology of quality management in clinical laboratory should be constantly updated in order to ensure the patient safety.The article focuses on the integration of risk management and six sigma,into the quality management system for clinical laboratory.

Objective To develop a bar code management system for two-way communication between XE-2100 hematology analyzer and LIS.Methods The relevant databases/tables were set based on PowerBuilder 7.0.The bar code ActiveX MSBCODE9.OCX was used for creating bar codes from patient information and test item information.Codes of all objects were translated to an executive file and DLL files.Results The results showed that the bar code was clear with high discernable rate,stable two-way communication and safe data storage.As the only identifier for specimen,the bar code improved work efficiency and management level,and avoided errors in sampling procedure.Conclusion The application of bar code technique to clinical hematology examination normalizes the workflow and provides a new mode for automatization and standardization management.

Objective To develop a self-help report printing system for management of inspection data and improvement of inspection workflow. Methods The self-help report printing system was compiled based on LIS with ORACLE database management system and was coded with PowerBuilder9.0 development kit. Results The information exchange in self-help report printing system and LIS are implemented successfully, and can print out inspection report with electronic signatures. Conclusion The self -help report printing system simplifies the reporting process and improves work efficiency through application in hospital.

Objective To perform the comparative analysis on the test results acquired from three different types of hematology analyzers ,to explore their results comparability or whether their deviations being within the allowable range .Methods The 30 d in‐ternal quality control results collected from the three types of analyzers ,Sysmex XE2100 ,Sysmex XN3000 and Sysmex XT4000i , were analyzed and analyze .The XE2100 hematology analyzer with excellent results in repeatedly participating in the national exter‐nal quality control assessment was taken as the reference instrument ,while the Sysmex XN3000 and Sysmex XT4000i analyzers served as the contrastive instruments .Four blood samples from different patients with high ,medium and low values were randomly selected and detected for 5 d .The results of white blood cell(WBC) ,red blood cell(RBC) ,hematocrit(HCT) ,hemoglobin(HGB) and platelet(PLT) were performed the comparative analysis .Then the accuracy and comparability of the detection results were judged by F test ,regression equation and correlation coefficient .Results The detection result of WBC ,RBC ,HCT ,HGB and PLT had no statistical differences among three instruments (P> 0 .05) .The correlation of various indexes had close correlation (r≥0 .975) .The deviations conformed to the related requirements with comparability .Conclusion When conducting detection by multi‐ple instruments ,the quality control of experimental instrument should be paid attention to ,the different detection instruments should be conducted the calibration and comparison at regular intervals for ensuring the accuracy and consistency of detection re‐sults ,thus better serve clinic .

This paper introduces such information of a urinary sediment diagnostic system and its data management system saving data directly from urine analyzer and microscope as its hardware organization and software design.With the performances of easy tooperate and low cost,the system can be applied toclinical urine diagnosis and togenerating standardized reports,whose data management system has high security and accuracy for saving data,strong anti-interference capability,reliable performance,noerror in proceeding and Chinese interface.

Objective:Using PCR to amplify filamentous fungi DNA fragments. Methods:Pour some liquid nitrogen to the hypha and grind them to powder. Then add some TE buffer and boil them for 15-20 minutes. Take a little supernatant as PCR template. After PCR do agarose gel electrophoresis. Collect the gel containing the objective fragment and add some TE. Take a little supernatant as PCR template (again.) After PCR, do agorose gel electrophoresis again. Results:We obtained a great deal of objective DNA. Conclusion:Amplifying filamentous fungi DNA fragment by this way, we can save time and money. We can also improve our work efficiently.