[Objective]This study investigated the utility of plasma D-dimer level as a diagnostic test for deep vein thrombosis (DVT)in patients for stroke rehabilitation.[Methods]Plasma samples were drawn from 109 nonambulatory rehabilitation patients recent ischemic or hemorrhagic stroke and assayed for D-dimer with D-Dimer PLUS method.Samples were drawn within 24 hours of venous duplex ultrasound(VDU)screening for DVT.[Results]Five of 105 patients had DVT identified by VDU scan.The op- timal cut point for predicting DVT was D-dimer 500?g/L,resulting in 100% sensitivity,89% specificity,31% positive predictive value,and 100% negative predictive value.[Conclusion]Plasma D-dimer level is a simple and inexpensive screening test for DVT during stroke rehabilitation.

This paper introduces the latest developments of the monitoring techniques for electroencephalogram (EEG) bispectral index (BIS), pulse indicator continuous cadiac output (PiCCO), entropy index (ENTROPY), internal organs blood stream perfusion (TONO), neuromuscular transmission (NMT) and spirometry, and their clinical applications.
Electroencephalography ; methods ; Entropy ; Heart Function Tests ; methods ; Humans ; Monitoring, Physiologic ; methods ; Neurologic Examination ; methods ; Respiratory Function Tests ; methods ; Signal Processing, Computer-Assisted

The key and difficult point always on the way of projecting the rural cooperative medical system is to find out a reasonable fund-raising level and compensating rate according to local economic condition.A variety of trials and researches on the fund-raising system have been committed in Xihu district of Hangzhou,Zhejiang province since June,2003 when it was chosen as an experimental place for the new rural cooperative medical system.In this paper,two main patterns of fund-raising system are introduced with their effects compared as well.Based on the data-analyses,it comes the conclusion that the key to ensure the new rural cooperative medical system's stable execution and sustainable development is to establish a reasonable and long-lasting cooperative fund-raising system.Some advices are put forward in the end.

[Objective]To explore the clinical effect of prolapse of waist intervertebral disc treated with traction and massage.[Method]382 cases were randomly divided into treatment group (traction and massage) 272 cases and controlled group of (traction) 110 cases for comparison.[Result]After 15 days for clinical effect,treatment groups were better control.[Conclusion]Prolapse of waist intervertebral disc treated with traction massage was better than prolapse of waist intervertebral disc treated with traction.

OBJECTIVE To study the inhibitory effect and mechanism of curcumin and its derivatives A and B on TGF-β induced LX-2 cell fibrosis. METHODS Established the liver fibrosis model of LX-2 cells induced by TGF-β(10 ng·mL−1).The effects on cell proliferation were detected by CCK-8. The effects on cell apoptosis was detected by flow cytometry. The effects on fibrosis related factors(Collagen I, Collagen Ⅳ, Fibronectin, Vimentin, α-SMA, PDGFRβ, TGFβR1, TGFβR2, MMP2, MMP9, TIMP1 and TIMP2) protein expression and gene transcription levels were detected by Western blotting and q-PCR. RESULTS The curcumin and its derivative A and B had the inhibition effects on normal LX-2 cells, and the IC25 values were 15.7, 2.6, 10.2 μmol·L−1, respectively. Compared to the model group, the curcumin(15.7 μmol·L−1) and its derivative A(2.6 μmol·L−1) and B(10.2 μmol·L−1) had the significant inhibition effects on cell proliferation of the TGF-β induced LX-2 cells(P<0.05). The cell apoptosis rate of curcumin derivative B group was higher than the model group(P<0.05). Collagen I, Fibronectin, Vimentin, α-SMA, TGFβR1 and TIMP-1 protein expression levels in curcumin group were lower, while the protein expression level of MMP-9 was higher(P<0.05). The protein expression levels of Collagen I, Collagen IV, Fibronectin, Vimentin, α-SMA, TIMP-1 and TIMP-2 in curcumin derivative A group were lower, while the protein expression level of MMP-2 was higher(P<0.05). The protein expression levels of Collagen I, Collagen IV, Fibronectin, Vimentin, α-SMA, PDGFRβ, TGFβR1, TGFβR2, TIMP-1 and TIMP-2 in curcumin derivative B group were lower, while the protein expression level of MMP-2 was higher(P<0.05). The gene transcription levels of Collagen I, Fibronectin, α-SMA and TIMP-1 in curcumin group were lower(P<0.05). The gene transcription levels of Collagen I, Fibronectin and α-SMA in curcumin derivative A and B groups were lower(P<0.05). CONCLUSION Curcumin and its derivatives A and B inhibit the abnormal activation and proliferation of TGF-β-induced LX-2 cells, inhibit the excessive secretion and accumulation of its extracellular matrix components, and promote its degradation, thus playing an anti-fibrotic effect in vitro, especially the curcumin derivative B.

There are five-year transitional period for manufacturers after the issue of regulation(EU) 2017/746 on
European Union ; Marketing ; Reagent Kits, Diagnostic

The olfactory disorders are mainly studied in the field of otolaryngology, but less in plastic surgery. In this review, the author summarized the anatomy of olfactory system, the etiology of dysosmia, the test of olfactory function, the relationship between dysosmia and nasal plastic surgery, and the treatment and prevention of dysosmia, in order to explore the relationship between olfactory disorders and plastic surgeries, especially rhinoplasty.

Objective To gain insight on how exercise affects the outcomes of prostate cancer patients treated with androgen deprivation therapy, specifically cancer-related fatigue (CRF) and quality of life (QoL).Methods Systematic searches for randomized clinical trials (RCTs) evaluating the effects of exercise on CRF and QoL of prostate cancer patients receiving androgen deprivation therapy were carried out to identify the eligible studies from EMBASE, PubMed and Cochrane library. Related data were extracted from eligible studies and then subjected to Reviewer Manage 5.3 for analysis. Standardized mean differences (SMD) and its 95% confidence interval (CI) were calculated.Results In all, 10 RCTs involving 841 prostate cancer patients (448 of whom exercised and 393 did not) were included in this study. With respect to CRF, there was good consistency among different studies, and it was remarkably reduced in the exercise group (SMD=-0.32, 95% CI: -0.45 to -0.18, P<0.00001, n=784). In regards to QoL, there was also good consistency among different studies, and it was also improved significantly in the exercise group (SMD=0.21, 95% CI: 0.08 to 0.34, P=0.002, n=841).Conclusion Exercise both reduced CRF and improved QoL in prostate cancer patients receiving androgen deprivation therapy.
Exercise Therapy ; methods ; Fatigue ; etiology ; physiopathology ; therapy ; Humans ; Male ; Prostatic Neoplasms ; physiopathology ; therapy ; Quality of Life ; Randomized Controlled Trials as Topic

OBJECTIVETo investigate the effects of different concentrations of paraquat (PQ) poisoning on the expression of voltage-dependent anion channel (VDAC) and caspase family in the mitochondria of rat lung tissue, and to explore possible mechanisms of acute lung injury induced by acute PQ poisoning.

METHODSTwo hundred healthy adult Wister rats with equal numbers of male and female ones were randomly and equally divided into control group and poisoned group. The control group received one-time gastric lavage with 1 ml of normal saline, and the poisoned group with PQ (50 mg/kg) diluted in 1 ml of normal saline. Twenty rats were collected at 1, 24, 72, 120, and 168 h after lavage with normal saline or PQ and dissected after anesthesia. Mitochondria were separated from rat lung tissue, and the content of VDAC and caspase-3, -8, and -9 were determined.

RESULTSThe expression of VDAC and caspase-3, -8, and -9 in the poisoned rats were significantly higher than that in the control group (P < 0.001). At 1, 24, 72, 120, and 168 h after exposure, acute diffuse damages were found in alveolar capillary endothelial cells, alveolar epithelial cells, and pulmonary interstitial cells. Inflammatory cell infiltration in the pulmonary interstitium, alveolar structural disorder, and substantially increased fibroblasts were also found in rat lung tissue.

CONCLUSIONPQ poisoning can up-regulate the expression of VDAC and caspase-3, -8, and -9 in mitochondria of rat lung tissue to induce acute lung injury.

Acute Lung Injury ; chemically induced ; pathology ; Animals ; Caspase 3 ; metabolism ; Caspase 8 ; metabolism ; Caspase 9 ; metabolism ; Caspases ; metabolism ; Female ; Lung ; drug effects ; pathology ; Male ; Mitochondria ; drug effects ; metabolism ; Paraquat ; poisoning ; Rats ; Rats, Sprague-Dawley ; Voltage-Dependent Anion Channels ; metabolism

OBJECTIVETo observe the effect of lipopolysaccharide(LPS) and interleukin-1beta (IL-1beta) on the expression of inducible nitric oxide synthase (iNOS) gene and nitric oxide (NO) in human periodontal ligament cells (hPDLCs).

METHODSAfter stimulating hPDLCs by LPS and IL-1beta, RT-PCR had been used to identify the expression of iNOS gene. The activity of iNOS in the culture was quantitated by enzyme-linked immune sorbent assay (ELISA). And the level of NO was determined by nitrate reductase method. RESULTS Slight amount of iNOS and NO had been detected in hPDLCs without sitimulation, but when stimulating with LPS and IL-1beta, the amount of the iNOS mRNA increased significantly in the cells in the time and dose dependent way (P<0.05). Under the stimulation with the same time or same dose, the productions of iNOS and NO stimulated by IL-1beta and in combination with LPS were larger than that stimulated with LPS (P<0.05).

CONCLUSIONThe expression NO and iNOS could be increased by stimulating hPDLCs with LPS and IL-1beta, which may contribute to the research on injecting LPS and IL-1beta at periodontal tissue of animal models.

Animals ; Humans ; Interleukin-1 ; Interleukin-1beta ; Lipopolysaccharides ; Nitric Oxide ; Nitric Oxide Synthase Type II ; Periodontal Ligament ; RNA, Messenger