Objective:To evaluate the effects of erythropoietin (EPO)on the proliferation and osteogenic differentiation of human dental pulp cells(hDPCs)in vitro.Methods:Isolated hDPCs were cultured and identified.The cells were treated by EPO and the proliferation of the cells was determined by CCK-8 assay.After incubation with EPO at 20 U /ml in osteogenic induction medium for 7 and 1 4 days,the mineralization of hDPCs was observed by alkaline phosphatase (ALP)activity assay and alizarin red staining.Real-time PCR was utilized to detect the expression of odontogenesis-related genes.Results:EPO enhanced the proliferation of hDPCs in a time-and dose-dependent manner.After treatment with EPO,ALP activity and the minerialized nodes of the cells increased(P <0.05);the expression levels of odontogenesis-related genes DSPP,OCN,OSTERIX and RUNX2 were upregulated(P <0.05).Con-clusion:EPO can promote proliferation and differentiation of human dental pulp cells.

A total of 118 patients (138 knees) who received total knee arthroplasty at the Department of Orthopedics, Guizhou Province People's Hospital between June 2000 and January 2007 were included in this study. These patients comprised 45 males and 73 females and were averaged (61±8) years old (range, 52-81 years). Of them, 28 (34 knees) suffered from rheumatoid arthritis, 6 (6 knees) from traumatic arthritis, 84 (98 knees) from severe osteoarthritis, 14 (18 knees) complicated by extraversion, and 25 (28 knees) complicated by inversion. Link Gemini knee prosthesis was used in 38 knees, Zimmer Nexgen knee prosthesis in 68 knees, and Depuy pfc sigma rotation platform knee system in 32 knees. All patients were followed up for an average of 28 months (range, 10 months-6 years). Prior to replacement, the mean knee score, according to the Hospital for Special Surgery (HSS) rating scale, was (34.00±7.65) points, and at the latest follow-up examination, it was (81.00±6.89) points. HSS score was excellent in 87 knees (63%), good in 43 knees (31%), and fair in 8 knees (6%). The excellent and good rate was 94%. Of 118 patients, 4 presented with infection and 3 showed deep venous thrombosis in lower limb following unilateral total knee arthroplasty and healed after anticoagulation and symptomatic therapy. There were 3 patients presenting with knee joint pains 2 months following knee joint arthroplasty. No knee joint red swelling and tenderness were found. Range-of-motion was basically normal. X-ray photograph indicated good position of prosthesis. Pains were relieved after femoral nerve block. All these findings suggest that total knee replacement is an effective method of treating severe knee joint diseases. INTRODUCTIONTotal knee arthroplasty (TKA), as an important part of artificial joint replacement for treatment of severe knee joint diseases, initiated from the first knee joint prosthesis developed by Cunstno in 1960s and was put in use. But following TKA, large-scale severe complications appear due to condition limitations, which results in failed surgery. With development of molecular biology and materials, as well as further research of installing technique and knee joint biomechanics, TKA has been shown to be gradually perfect theoretically. A novel prosthesis better corresponds to the physiological standards of human body in terms of structure design, material strength, and shape. These improvements greatly enhance prosthesis survival rate and markedly decreases complication incidence following TKA. TKA has been shown to be an efficient method of treating severe knee joint diseases. It can effectively relieve joint pains and improve joint functions, and is mostly used in elderly populations (over 60 years old) who complicated by other diseases, such as diabetes mellitus, hypertension, heart disease, phlebitis of lower extremities. In addition to above-mentioned, poor body resistance enhances surgery risk and difficulties, and complications would be correspondingly increased following TKA. The present study retrospectively analyzed a total of 118 patients with severe knee joint diseases who underwent TKA at the Department of Orthopedics, Guizhou Province People's Hospital between June 2000 and January 2007.

The overall situation of projects of ethnomedicine and ethnopharmacology funded by the National Natural Science Foundation of China (NSFC) since 2008 has been presented in this paper. The main source of characteristics and innovation of the funded projects were summarized, which may come from several aspects, such as the ethnomedical theories, the dominant diseases of ethnomedicine, special diseases in ethnic minorities inhabited areas, unique ethnomedical therapy, special methods for applying medication, endemic medicinal materials in ethnic minorities inhabited areas, same medicinal materials with different applications. Examples have been provided to give references to the applicants in the fields of ethnomedicine and ethnopharmacology.
Biomedical Research ; economics ; organization & administration ; China ; Ethnopharmacology ; economics ; organization & administration ; standards ; Financial Management ; Humans ; Medicine, Traditional ; economics ; standards ; Natural Science Disciplines ; economics

Notch signaling pathway plays a vital role in cell fate decisions. Notch signals are transferred among adjacent cells through Notch receptors and their ligands, which can regulate differentiation, proliferation and apoptosis of many cell types including stem cells. They can also influence organ formation and morphopoiesis. Genetic mutations in the Notch signaling pathway are related to the emergence and development of many diseases. Notch signaling pathway is increasingly becoming important drug targets for cancer, hereditary disease such as CADASIL and other related diseases. It is also used to develop stem cell therapeutics to treat age or trauma related degenerative diseases such as Alzheimer's disease, Parkinson disease and diabetes.

Objective To evaluate the CT manifestation of pancreatic carcinoma proved by patholoical results. Methods The patients were supine. Both plain and three-phase dynamic contrast-enhanced CT scan were performed. 100 ml of Ominipaque was injected at a rate of 2～4ml/s, with 5~10 mm slice thickness and interval. Results CT scanning clearly showed the direct signs or indirect signs of pancreatic carcinomal, which include: pancreatic mass, obstructive dilatation of the bile duct and pancreatic duct, the visualization of the abnormal large peripancreatic vessels, lymph node metastasis, etc. Conclusion Plain CT scanning shows clearly manifestation of pancreatic carcinomal and the surrounding tissue, and three-phase dynamic contrast-enhanced CT scan can clearly display the tiny changes of pancreatic parenchyma. Therefore it is valuable for diagnosis of pancreatic carcinomal.

The involvement of oxidization of peroxid in the antigen-antibody reaction makes the cell membrane permeability enhanced, so the fluorescence-labeled antibody stain can infiltrate into the cell easily and contact the antigen rapidly and diffusely, and then the antigen-antibody crosslinking can be formed efficiently. With the enhanced staining efficiency, shortened test duration, simplified operation, increased positive detection rate and accuracy, the new technology lays a foundation of clinical definite, curative effect view and prognosis.

Objective: To evaluate the importance of waist to hip ratio among all the possible risk factors in the development of NAFLD. Methods: The samples were selected from individuals receiving physical examination from the 14th of October to the 4th of November in 2004.The enrolling criteria were as follows:①BMI between 25-29.9.②ethanol consumption was less than 40 g per week.③markers of hepatitis virus were negative.④no diabetes mellitus.The diagnosis of fatty liver was based on sonographic evidence.According to the standards,81 cases were chosen,including 59 patients with NAFLD and 22 normal controls.Seven indices were recorded in fasting state: age,BMI,waist to hip ratio(WHR),serum cholesterol,serum lipid,serum glucose and insulin sensitivity index.Results: The fatty liver group,compared with the normal controls,was significantly marked with higher BMI(26.70 vs 26.15,(P=)0.026), WHR(0.90 vs 0.86,P=0.002),serum lipid(1.99 vs 1.42 P=0.001) and lower insulin sensitive index(0.025 vs 0.039,P=0.005).By binary logistic regression analysis,WHR and insulin sensitive index is significantly associated with the development of NAFLD,and the P value is 0.01 and 0.069 respectively. Conclusion: WHR is an independent factor in the development of NAFLD in patients with BMI matched and non-DM mild fatty individuals.

To meet the requirements of military medical readiness and to win in informatized war, striding development of Chinese medical equipment has to be performed. This paper presents related guiding ideology, principle, mission, objective and countermeasures.

Objective To investigate the effects of continuous nursing quality improvement on emergency care of parturient with preeclampsia.Methods The parturient with preeclampsia in the First Affiliated Hospital of the Medical University Of Anhui from January 2010 to December 2015 were chosen.The effects of continuous nursing quality improvement on the emergency care of those parturient were analyzed and the maternal and infant health outcomes and patient satisfaction were contrasted.Results The surgical complication rate of the observation group patients was significantly lower than that of the control group (P＜0.001).The overall health outcome of maternal and infant patients in the observation group was significantly better than that of the control group (P＜0.000) Patient satisfaction of the observation group was significantly higher than that of the control group (P＜0.000)Conclusion Continuous nursing quality improvement model can not only ensure the quality of eclampsia patient care but also keep on improving the care quality so as to improve maternal and infant health outcomes and patient satisfaction.