Objective To explore the factors associated with infections in children with leukemia after chem-otherapy. Methods Clinical data of fifty-six children with leukemia, who infected after chemotherapy were reviewed with infection characteristics and some related factors. Results The infection rates were 27% in upper airway,37% in lower upper airway,12% in oral cavity,5% in gastrointestinal tract,3% in perianal abscess,7% in urinary sys-tem,5% in scpficaemia and 2% in ofitis media. Most infection happened from five to ten days after chemotherapy,and the infection rates varied with chemotherapy scheme. The persistence time of infection were (8.5±2.2) days in the children with the numbers of neutrophil below 0.5×109/L and (3.5±1.4) days in those above 0.5×109/L with significant difference(P <0.01). Conclusions After chemotherapy,the children had more chances to be infected. To be more exact, the /nfection was more common observed in respiratory tract, next in gastrointestinal tract. And the stronger the medicine used the number of heterophil granulocyte dropped much more ,the infection happened more easily.

Objective To explore the effect of transforming growth factor ?_1 (TGF-?_1) on expression of paxillin(Pax) in human kidney proximal tubular epithelial cell line (HKC cells) in different phases. Methods HKC cells cultured in vitro were divided into four groups at random. Control group(C group): HKC cells were cultured with serum free medium (FSM), and TGF-?_1-treated groups (T1, T2, T3 group): HKC cells were cultured with FSM containing 10ng/ml TGF-?_1. In the latter groups, duration of treatment varied as follows: T1 group for 24h, T2 group for 48h, T3 group for 72h. The proliferation of HKC cells induced by TGF-?_1 was assessed by MTT and the expression of Pax was determined. The gene expression of Pax was determined by reverse transcripton polymerase chain reaction (RT-PCR) and the protein expression of Pax was assessed by immunohistochemical staining and Western blot. Results TGF-?_1 could induce the proliferation of HKC cells in a time-dependent manner. In C group there was expression of Pax mRNA and protein in HKC cells. The expression of Pax mRNA and protein in HKC cells was positive in all the T groups, especially in T2 group and T3 group they were significantly increased compared with T1 group (P

Objective To investigate the expression of Paxillin (Pax) in human kidney proximal tubular epithelial cell line (HKC) induced by transforming growth factor ?1(TGF?1). Methods HKC cells cultured in vitro were divided into three groups at random: control group(C): cultured with free serum medium(FSM), TGF?1-treated groups (T1 and T2): cultured with FSM containing different concentrations of TGF?1 (T1: 5 ng/ml, T2: 10 ng/ml). After 48-hour treatment, the expression of Pax mRNA and protein was detected by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemical staining in HKC cells respectively. Results In Group C, the expression of Pax mRNA and protein in HKC cells was elementary. However, in T1 and T2 groups , the expression of Pax mRNA and protein in HKC cells was greatly increased, especially in T2 group which was more significantly increased than T1 group (P

Objective To explore the cognition processes of language in stroke patients by characterizing the differences between the event-related potentials (ERPs) N400 evoked bypicture-word andsentence-wordparadigms.Methods Twenty patients with a single brain lesion resulting from a first episode of stroke were tested along with 20 normal controls.Their N400 ERPs were tested using pictures and sentences as activating stimuli and phrases composed of nouns and verbs as the target stimuli.The N400 potentials were recorded from electrodes located at Fz,Pz,T3 and T4 positions of the International 10-20 system.The N400 latencies and amplitudes and the behavioral responses of the experimental and control groups were compared.Results The latencies were longer and the amplitudes were lower in response to sentence activation than to picture activation.The differences in terms of amplitude were the greatest with N400 recorded from Pz.The responses to the target stimuli were not significantly different with regard to the types of stimuli and recording location.The average response time was longer and the error rate was higher in response to sentence activation than to picture activation.Conclusion Multiple accesses,activating stimuli and integrating processes are involved in language cognition.The brain is more sensitive and accurate in response to picture stimuli in language cognition.The response to sentence stimuli is slower,and the error rate is higher.This suggests that it is better to usc varied prime stimuli in teaching children languages.

Lycopene is an important natural carotenoid,and is widely found in human tissues and blood,with the prevention of many types of cancer,anti-aging effect.It can inhibit the proliferation of cancer cells of oral malignancy,and increasing of gap-junction communication between cells to prevent oral cancer,and also can inhibit the development of colorectal cancer by reducing the activity of matrix metalloproteinase.It plays the role of anti-metastasis through enhancing the expression of anti-metastatic gene nm23-H1.

Recently,many studies find that microRNA plays a key role in the development,diagnosis,treatment and prevention of gastric cancer.It is confirmed that miR-451 can be used as a useful biomarker for the screening of gastric cancer.Studies also show that miR-203 and miR-21 may become diagnostic markers of gastric cancer.MicroRNA can play the role of oncogenes,and also can play the role of tumor suppressor gene,such as miR-141 plays the role of tumor suppressor gene through inhibition of the positive fibroblast growth factor receptor 2 gene expression.

Objective To study the mRNA expression of MCT1 and MCT2 genes in human hepatocellular carcinoma (HCC) and paracarcinoma liver tissue (PCLT). Methods The semi-quantitative analysis of MCT1 and MCT2 genes mRNA expression in human HCC and PCLT was conducted by RT-PCR method and electrophoresis band opacity density (OD) comparison analysis method in 25 patients with HCC. Results The mRNA expression of MCT1 was significantly higher than MCT2 in HCC and PCLT, in HCC the mRNA expression of MCT1 and MCT2 genes were significant higher than that in PCLT. Conclusions The high expression of mRNA of MCT1 and MCT2 genes in HCC indicates that these genes may take a significant role on lactate and other monocarboxylate transmembrane transportation and on pH regulation in tumor cells.

OBJECTIVE To investigate the characteristics of mycoplasma infection and antifungal resistance to guide the clinical reasonable usage.METHODS Mycoplasma was isolated and its antibiotic susceptibility was detected by Mycoplasma IES test kit.RESULTS Among the 1103 samples,418(37.8%) were positive,including 268 with Ureaplasma urealyticum(Uu)(64.1%),13 with Mycoplasma hominis(Mh)(3.1%),and 137 with mixed infection(Uu and Mh)(32.8%),Young people with the age from 20 to 45 were the highest group of infection(80.1%),Sensitive antibiotics to mycoplasma in precedence order were doxycyline,minocycline,tetracycline,josamycin,and clarythromycin.CONCLUSIONS Mycoplasma is a common pathogen resulting in urogenital tract infection.We should pay more attention to monitor mycoplasma infection and use antibiotics properly and to guide clinically antibiotics usage.

Objective To design an intravenous indwelling needle in battle field aid.Methods Several concavity were made on the hollow tube inner surface posterior puncture needle case,and some corresponding convexity uplifted peripheral needle handle pistons posterior puncture needle core.Revolution could be avoided because the needle handle pistons were just embedded in hollow tube concavity.It was convenient to operate and decreased the operator's orientation transformation by the needle's handhold vane adopting rectangle instead of butterfly shape.Results The improved puncture needle was stable and the rate of success of intravenous puncture was increased.Conclusion The improved venous retaining needle can be applied in battle field aid.

Objective To explore the effect of heroin dependence on the expression and the function of glucagon in islet A cells of rats.Methods The changes of numerical density on area of A cells and level of plasma glucagon of heroin-dependent rats were investigated by means of the immunohistochemical SABC method,image analysis and radioimmunoassay(RIA).Results As compared with SCG and NCG,it was observed that the number of Glu-IR positive cells increased and the intensity of immunohistochemical staining enhanced in the heroin-dependent groups.The result of image analysis showed that the numerical density on area of A cell apparently increased in the 38-day heroin-dependent group(P