Objective　To evaluate the in vitro effect of che motherapeutant on hepatic carcinoma.　Methods　The inhibitory effect of 5-Fu, DDP and 4-PA on Bel-7402 cel ls proliferation was studied by MTT colorimetry.　Resu lts　(1) Bel-7402 was on an active proliferation by the treat ment of 5-Fu and DDP for half an hour, and the proliferation inhibited after 12 hours; but the proliferation was inhibited by 4-PA after half an hour of tr eatment. (2) The inhibitory rates increase with time and were over 50% after 48 ～72 hours of treatment. (3) There was temporarily active proliferation of Bel- 7402 cells in the early period of chemotherapy by 5-Fu and DDP. 　Conclusion　Continuous chemotherapy is effectiv e for the inhibition of Bel-7402 cells proliferation.