Bacterial infection is likely to develop into sepsis, which is regarded as the main reason for high mortality rate. High-density lipoprotein cholesterol (HDL-C) and its associated apolipoprotein can combine with lipopolysaccharide, regulate the body's inflammatory response and reduce the mortality, which can provide a new method for treatment of bacterial infection.

Anoikis,a specific form of programmed cell death,is triggered by cell detachment from extracellular matrix or adjacent cell.Studies have found that the most kinds of tumor cells had anoikis resistance characteristic,which may inhibit pro-apoptosis protein,block anoikis inside and outside pathways,up-regulate pro-survival factor signals,and promote survival,invasion and metastasis of tumor cells in the end.The review summarized the mechanism of anoikis and the relationship between anoikis and tumor invasion and the metastasis.

Objective To evaluate the effectiveness of bio-safety theory intensive training before laboratory medicine intern-ship.Methods Collected 220 students of three universities in human before laboratory medicine internship,according to dif-ferent university,and the different grade of the same university,and the same class grouping pre-and post-the bio-safety in-tensive training,the obj ects of study in accordance with the training scheme to do on-site questionnaire survey by secret way in three universities,comparing the effectiveness pre-and post-training.Results Three universities had no difference before training of laboratory medicine students (χ2=0.081~3.135,P>0.05).However,in the same university,pre-training,the basic concepts and microbiological hazards assessment could be better grasped (respectively,P=0.000,0.000).Post-train-ing,the basic concepts and bio-safety operation specification could be promoted (respectively,P=0.000,0.002).In the same class between pre-and post-training,test results had no difference (χ2=0.096~2.408,P>0.05)except personal protec-tive equipmentin 2008 grade students,while in 2009 grade test performance was improved (χ2=4.821~12.27,P<0.05) except the basic concepts.Conclusion Bio-safety intensive training has good effects to operation skills,according to the spe-cific situation of students targeted training,which made students real benefit,and ensure laboratory medicine students’labo-ratory safety.

Objective To evaluate the clinical value of 9 9 Tc m-HL91 as a tumor positive imaging agent in diagnosis of pulmonary neopl asms. Methods We performed 2 h,4 h planar and 4 h tomogr aphy fusion imaging after injection of HL91 on 42 patients with high probability of pulmonary cancer. Tumor-to-normal tissue background (T/N) ratios were meas ured for all scans. Results T/N ratios at 2 h,4 h planar and 4 h transectional imaging in malignant group were 1.50? 0.17 ,1.71?0.22 and 2.72?0.75, respectively; while in benign group, they were 1.21?0.10,1.25 ?0.22 and 1.49?0.36, respectively. 4 h delay T/N ratios in malignant group we re obviously higher than 2 h ( P 0.05). There were no remarkable differences of T/N ra tios in different phases and histological types of lung carcinoma. Using maligna nt transection ratio ( -1s ) as diagnostic threshold, the sensitivity , specificity and accuracy were 84.85% , 88.89% and 85.71% , respectiv ely. Using transection ratio( +1s ) of benign group as diagnostic th reshold , the sensitivity, specificity and accuracy were 90.91%, 88.89% and 95.2 4%,respectively. Conclusion The results indicate that as a tumor positive imaging agent 99 Tc m-HL91 is useful in diagosising pulmon ary neoplasms.

Objective To certify whether 99mTc-HL91 can be trapped in ischemic myocardium prominently for clinical imaging. Methods The model of myocardial ischemia in rabbits was prepared and the distribution of 99mTc-HL91 was observed. The distribution of 99mTc-HL91 in the whole body was also observed. Results The myocardium of the left ventricle supplied by LCX was ischemic according to the change of electrical physiology and the results of NBT dying. The radioactivity in the myocardium of left ventricle supplied by LCX was higher than that supplied by LAD. LCX/LAD: 120 min, 2.57; 180 min, 3.45. 99mTc-HL91 was mostly distributed in the liver and kidney, next, the stomach and intestine. It was excreted through urination and defecation. There was little radioactivity in the heart and lungs and it eliminated quickly. 99mTc-HL91 was eliminated quickly from blood, too. Conclusion Ischemic myocardium can trap 99mTc-HL91 more significantly and relatively prominently at 180 minutes of post-injection. 99mTc-HL91 can be used in myocardial hypoxia imaging.

Objective To investigate the factors affecting non-neoplasma touch technology operation by operation room nurses for malignant tumor patients.Methods The qualitative methods was used in the study to interview 15 operation room nurses phenomenologically using semi-structured interviews.Colaizzi principles were used for data analysis and summarization of themes.Result The themes brought out from the data included lack of knowledge of no-neoplasma touch technology operation,huge workload,less importance attached to the management,over-consideration of medical costs.Conclusions It is a need to improve the level of knowledge of operating room nurses on non-neoplasma technology operations.The importance should be attached to management of the technology operation.The training on the operation should be strengthened so as to improve their operation of the technology.

Objective To better understand the clinical characteristics of Familial Idiopathic Basal Ganglia Calcifi?cation (FIBGC), including at the perspective of hereditary pattern, clinical test results, onset age, clinical heterogeneity and the volume of basal ganglia calcification (VBGC). Method 8 Eight FIBGC families were collected and draw family pedigrees were draw. Analysis of was conducted on the patient's clinical test results, head CT and MRI changes, onset ag?es, relationship of clinical manifestations with VBGC. Results No significant difference was found in serum calcium, alu?minum, arsenic, cobalt, magnesium, phosphorus, iron, parathyroid hormone and calcitonin concentration between the fam?ily members of patients and healthy controls (P>0.05). Family members from 8 FIBGC families including the two with consanguineous marriage manifested autosomal dominant heredity. The severity of , symptomatic s was correlated with VBGCpatients showed the same clinical manifestations in the dyskinesia family. The psychiatric symptoms was not asso? ciated with VBGC whereas patients with dyskinesia had a large VBGC. There was a significant difference in onset age be?tween patients with psychiatric symptoms and those with dyskinesia. P.atients with dyskinesia suffer larger VBGC, and is characterized by Patients with dyskinesia had relatively later onset age (43.95 ± 2.47 y) whereas those with. psychiatric symptoms hadsymptomatic patients with early onset age (31.32±10.16y). The comparison of the onset age (43.954±2.473 vs. 31.319±10.156 y, t=4.438, P=0.001) and VBGC (1.748±0.622 vs. 0.392±0.276 cm3, t=2.518, P=0.028) with symptom?atic patients between dyskinesia and psychogenic families was significant. Conclusions Eight FIBGC families manifested autosomal dominant heredity. Patients with dyskinesia suffer have a larger VBGC and are associated with a, and is char?acterized by relatively later onset age. In contrast, patients with psychiatric symptomspsychogeny is not related withhave a the small VBGC and showedand their age of onset is young. earlier onset age.

OBJECTIVETo analyze caries activity of nasopharyngeal carcinoma (NPC) patients before and after radiation therapy. The study was also designed to analyze calcium and phosphorus concentrations in saliva of NPC patients before and after radiotherapy.

METHODSTwenty-eight NPC patients and 20 healthy controls were included in this study. Resazurin disc method was used to detect caries activity of NPC patients before and after 70 Gy radiotherapy. The atomic absorption spectrometry and molybdenum-stibium antispetrophotography method were used to analyze calcium and phosphorus concentrations in saliva of NPC patients before and after 70 Gy radiotherapy and the healthy controls.

RESULTSThe results of resazurin disc method showed caries activity of NPC patients was increased after radiotherapy (P < 0.01). Calcium concentration in saliva of NPC patients before radiotherapy was (63.19 +/- 3.27) mg x L(-1), phosphorus concentration was (132.96 +/- 5.13) mg x L(-1). After 70 Gy radiotherapy, calcium concentration was (33.38 +/- 0.32) mg x L(-1), phosphorus concentration was (49.18 +/- 2.66) mg x L(-1). The difference of calcium and phosphorus concentrations in saliva before and after radiotherapy was significant (P < 0.01).

CONCLUSIONCaries activities of NPC patients increase after radiotherapy. Calcium and phosphorus concentrations in saliva decrease after radiation therapy.

Objective To investigate the accuracy of 320-slice row CT system for the detection of coronary artery disease(CAD)in high pre-test probability population without heart rate/rhythm control.Methods Thirty patients with a high pre-test probability of CAD underwent 320-slice row CT without preceding heart rate/rhythm control.Invasive coronary angigraphy(ICA)served as the standard reference.Data sets were evaluated by 2 observers in consensus with respect to stenoses≥50% decreased diameter.The sensitivity,specificity,positive predictive value(PPV),negative predictive value(NPV)and Youden index were analyzed;the impact of heart rate and calcification on image quality as well as diagnostic accuracy were also analyzed by Chi-square test Results Mean heart rate during scanning was 73.7±15.4 beats per min(bpm),and median(QR)of Agatston score of segment was 45.6(181).On a per-segment analysis,overall sensitivity was 96.1%(74/77,95%CI:89.03%-99.19%),specificity was 98.3% (337/343,95%CI:96.23%-99.36%),PPV was 92.5% (74/80,95%CI:84.39%-97.20%),NPV of 99.1% (337/340,95%CI:97.44%-99.82%)and the Youden index was 0.94.In both heart-rate subgroups(242 in heart rate＜70 bpm group,169 in heart ratet≥70 bpm group),diagnostic accuracy for the assessment of coronary artery stenosis was similar(P＜0.05).The accuracy and the quality score of the subgroup Agatston score≥100 were lower than that of the subgroup Agatston score＜100;however,the difiercnce of results between 320-slice row CT and ICA was not significant(P＜0.05).Conclusion 320-detector row CT can reliably detect coronary artery stenoses in a high pre-test probability population without heart rate/rhythm control.

OBJECTIVE: To observe the expression and distribution of transforming growth factor β3 (TGF-β3) in the mouse tooth germ after advanced bell stage, and to discuss the role of TGF-β3 during the development of tooth germs. METHODS: BALB/C's mouse tooth germs at 4, 11, and 18 days postnatal (4dpn,11dpn,and 18dpn) were collected and processed for routine fixation, decalcification, embedding, and slicing. The expression of TGF-β3 was detected by immunohistochemisty. RESULTS: As to 4dpn tooth germ: Positive expression of TGF-β3 was found in enameloblasts, odontoblasts, ambitus of dental pupilla, with weak positive expression in the intermedial of dental papilla. As to 11dpn tooth germ: Positive expression was seen in enameloblasts, with negative expression in odontoblasts and dental papilla. As to 18dpn tooth, positive expression of TGF-β3 was showed in the vessel wall and its surrounding, with negative expression in other areas. CONCLUSION The distribution of TGF-β3 expression showed a time-space characteristic during the mouse tooth germ development after advanced bell stage, which may exert a regulatory effect on tooth development and this effect is gradually getting weak with the development of tooth germs.
Animals ; Animals, Newborn ; Immunohistochemistry ; Mice ; Mice, Inbred BALB C ; Molar ; metabolism ; Tissue Distribution ; Tooth Germ ; growth & development ; metabolism ; Transforming Growth Factor beta3 ; metabolism