The pattern and distribution of vimentin intermediate filament in synovial cell in vitro were observed by means of immunohistochemistry,confocal scanning laser microscopy (CSLM) and three-di-mentional photograph reconstruction on adherent cell analysis and sorting (ACAS 570). The result showed that vimentin presented spongy stero-structure in whole cytoplasmic space. The reconstructed three-dimentional photograph was similar to the appearance of the cultured synovial cell. The relationship between the distribution of vimentin and the morphological change of cultured synovial cell in different growth period was discussed in this paper.

Objective To explore the roles of IL-8, sICAM-1 and sE- selectin in the airway inflammation of chronic obstructive pulmonary disease (COPD). Methods The levels of IL - 8, sICAM -1 and sE-selectin in the serum were measured by double-sandwich ELISA in 40 COPD patients and 20 healthy persons. The COPD patients were divided into two groups: 20 cases in COPD a-cute exacerbation group and 20 cases in stable period group. Results The IL- 8, sICAM- 1 and sE - selectin level of serum in patients with COPD acute exacerbation group and stable period group were obviously higher than those in healthy group, and patients with COPD acute exacerbation group was obviously higher than those in stable period group (P

AIM: To evaluate the effect of GYY4137, a novel hydrogen sulfide (H2S) donor, on cytosolic lipid decomposition in mouse primary steatosis hepatocytes.METHODS: Oleic acid (OA) was used to induce hepatic steatosis model in vitro.The C57BL/6 mouse primary hepatocytes isolated and cultured by 2-step in situ perfusion were divided into 4 groups: the cells in control group were incubated with normal medium for 54 h;the cells in model group were incubated with OA at 1.2 mmol/L for 48 h followed by serum-free phenol red-free RPMI-1640 for 6 h;the cells in H2S group or DL-propar-gylglycine (PAG;an inhibitor of cystathione γ-lysase, inhibiting H2S synthesis) group were incubated with OA at 1.2 mmol/L for 48 h followed by serum-free phenol red-free RPMI-1640 which contained 1 mmol/L GYY4137 or 200 μmol/L PAG for 6 h.The glycerin release and the protein expression of hormone-sensitive lipase (HSL) in the cells were mea-sured.RESULTS: Compared with model group, the glycerin release and the protein expression of phosphorylated HSL (p-HSL) in H2S group decreased significantly, while those increased significantly in PAG group.CONCLUSION: In steatosis hepatocytes, exogenous H2S possibly decreases cytosolic lipid decomposition by decreasing the protein level of p-HSL.

Objective To study the Diagnosis and treatment of thyroglossal duct cyst or fistula. Methods The clinical data of 67 patients with thyroglossal duct cyst or fistula treated by surgery were analyzed retrospectively. Results The diagnosis rate preoperatively was 92.5% and the accuracy diagnosis rate by B type ultrasonography was 96.1%; all cases were treated by operation, of them, 14 cases underwent simple cyst resection, 12 with resection of partial periosteum of hyoid and 41 with resection of middle segment of hyoid( Sistrunk's operation ). 54 cases were followed up, recurrence in cases with cyst was 4 of 43 (9.3%) and recurrence in cases with fistula was 2 of 11(18.2%)respectively. Conclusion Ultrasonography shows certain characteristics and clinical value to diagnose and distinguish thyroglossal duct cyst. Postoperative recurrence is due to the selection of operation procedures and control of infection of focus. The Sistrunk's operation properly performed previou to cyst infection should reduce recurrence rate markedly.