An outbreak of rabies took place in may 1987. At the begining, rabies disseminated among dogs, horses and oxen,then it passed to the human beings. This article describes the pathological features and problems related to its diagnosis by means of observation of microscope and electronmicroscope on the brain tissue.

Objective To study the distribution of larvae of Trichinella spiralis in muscles of infected mice.Methods The muscles of mice infected with Trichinella spiralis were examined with microscopy.These muscles included lingua,masseter,pectoral muscle,abdominal muscle,forelimb muscle,crus muscle,diaphragma and dorsal muscle.Results The densities of the larvae were highest in diaphragma and lowest in abdominal muscle.Conclusion The larvae infection rate of diaphragma was higher than that of other muscles of mice with Trichinella spiralis infection.

Objective To investigate the infection status of different adenovirus serotypes of adenovirus among normal popula‐tion in Shandong area in order to screen out the serotypes of low prevalence level for the use in the adenoviral vector construction . Methods Serum levels of neutralizing antibodies against different serotypes of adenovirus were determined with ELISA by using different adenovirus serotype standard strains .Results The positive rate of total adenovirus antibodies in the normal population aged 18-70 years old in Shandong area was 95 .2% ,in which the antibody positive rates of Adv3 ,Adv5 ,Adv7 ,Adv11 ,Adv26 , Adv35 ,Adv48 and Adv49 were 69 .8% ,71 .0% ,61 .0% ,9 .4% ,2 .2% ,5 .8% ,0 .8% and 1 .6% ,respectively .The positive rates of Adv11 ,26 ,35 ,48 ,49 neutralizing antibodies were significantly lower than those of Adv3 ,5 ,7 serotypes(P<0 .01) .Conclusion The adenovirus total infection rate is high in Shandong area .The infection rates of different serotypes are different ,in which Adv26 ,48 , 49 serotypes have lower infection rates and can be used for constructing the adenovirus vector .

Nine patients with neonatal malabsorption syndrome and low birth weight infants were treated with PPN support. 10 cases of newborns were as control. The results showed that increases in weekly average body weight and plasma albumin after treatment were higher in the therapeutical group than in control group ( P

Squeezing and smear method was used to detect Demodex infection for college students,the overall infection rate was 30.81% with Demodex folliculorum as the major one,D.brevis and mixed infection as the second ones.Certain relations were found between facial signs,lipid content and the infection.Prevalence was higher in those students who used to share washing materials(towels,etc.)with family members.

31 rat were divided randomly into control group (n=10),tripterygium wilfordii glycosides group (n = 10 9. 4 mg/kg per day),and poly saccharide sulphate group (n=11. 30 mg/per day). We made balloon endothelium denudation in thoracic aortae of the rats. The treatments with drugs began 6 days before balloon injury and continously until the animal were killed 14 day after balloon injury. The determinations for area of neointima, neointima /media, coverage of neointima and 3H-TdR incoperation of thoracic aortae suggested that tripterygium wilfordii glycosides can inhibit intimal proliferation of injuried aorta, whill poly saccharide sulphate shows no effect.

There were no data concerning restenosis rate after percutaneous transluminal coronary angio-plasty available in China so far. Our study was focused on clinical and angiographic follow-up and tried to reveal the predictive value for angiographic restenosis using noninvasive tests among 592 patients whose 841 coronary arteries were dilated in our department from Dec. 1987 to Dec. 1994.This patient group consisted of 503 males and 89 females had average age of 58. 4 ? 9. 1. The overall success rate was 93. 3% and acute vessel complications was 4. 1%. After successful procedures of PTCA,the patients were followed up with recurrence of angina, Holler ECG, submaximal excercise ECG and 99mTc-MIBI exercise scintigraphy regularly. 62 of these patients underwent coronary angiographic follow-up from 4-24 months (average time: 10. 4 ? 7. 6 months) after PTCA. We found 35 patients with angiographic restenosis and 27 patients without. Thus, we divided the patients into restenosis group and non-restenosis group according to the angiographic results. Subsequently, we oberserved whether the symptom and noninvasive tests were valuable in prediction of restenosis. The result showed that recurrence of angina, Holter ECG, submaximal exercise ECG and 99mTc-MIBI scintigraphy were useful in detecting restenosis with their specificity and sensitivity 75.7% and 72.0%, 71. 4% and 86. 7%, 66. 7% and 73. 1% ,76. 5% and 83. 3 %, respectively. Moreover, If any two of above four ischemic indexes were combined together to detect restenosis,the specificity and sensitivity were significantly enhanced (85. 2% and 92. 9%). It is concluded that recurrence of angina and the noninvasive tests were valuable in predicting restenosis. The combination of these ischemic indexes would greatly improve their predictive value.

Objective To study the association of peroxisome proliferator activated receptor?2 (PPAR?2) gene polymorphism with type 2 diabetes mellitus.Methods PPAR?2 gene Pro12Ala polymorphism was examined using polymerase chain reaction and restriction fragment length polymorphism (PCR RFLP) in 101 couples of type 2 diabetes mellitus normal control.Results In control group, heterozygosity carriers had lower serum apolipoprotein B (P=0 006).The Pro12Ala polymorphism in PPAR?2 gene is not associated with type 2 diabetes mellitus(OR=1.48,95%CI=0.52～4.18,P=0.461).Conclusion PPAR?2 gene Pro12Ala polymorphism is associated with abnormal lipid metabolism; PPAR?2 gene may not play a critical role in the development of type 2 diabetes mellitus in Han of Beijing Chinese.

Objective To study the treatment of renal artery stenting for patients with coronary artery heart disease and renal artery stenosis who need coronary atery bypass graft (CABG) and its effect on preventing acute renal dysfunction after CABG. Methods From April 2001 to January 2005, renal artery stenting was carried out in 82 patients (a total of 104 renal arteries) with CHD and renal artery stenosis who needed CABG. Among the 82 patients, 77 of them accompanied with hypertension and 21 of them had abnormal renal function. Heparin was selected as anticoagulant other than anti-platelet drug and low molecular weight heparin before stenting. Results All of the stents were successfully implanted in 104 renal lesions without complication. Blood creatinine (Cr) reduced in 9 patients with renal dysfunction by the time of discharge. Three patients had blood Cr increased provisionally after stenting. Four patients with normal pre-operational renal function showed transient increase in blood Cr after stenting. All of the patients with transient blood Cr elevation had bilateral renal artery stenosis. Conclusion Renal artery stenosis may cause acute renal failure after CABG. Pre-CABG renal artery stenting may prevent the occurance of acute renal failure after operation.

Objective To compare the immune protective effect of different antigens of Trichinella spiralis:excretory-secretory(ES) antigen of adult worms, ES antigen of muscle larva and their mixed antigens in mice. Methods ES antigens of Trichinella spiralis adult worms and muscle larva were produced by the physiological saline culture methods. The ES antigens of Trichinella spiralis adult worms, muscle larva and the mix were used to immunize mice 3 times at 7 day interval, adjuvant control and normal control were set up. Seven day after the final immunization, each mouse was orally challenged with 200 Trichinella spiralis larva. Intestinal adult worms and muscle larva of Trichinella spiralis of each group were recoveried and examined on Day 7 and Day 30 post-challenge,respectively. Results The intestinal adult worms of Trichinella spiralis reduced by 87.95%, 69.48% and 84.34% in the adult worm ES antigen group, muscle larval ES antigen group and their mixed antigen group, respectively, while muscle larva reduced by 74.79%, 87.97% and 86.87% respectively. Adult worm reduction rates of the adult worm ES antigen group and mixed antigen group were higher than those of muscle larval ES antigen group (P