Objection: To investigate the effects of tumor necrosis factor-?(TNF-?)and interferon-?(IFN-?) on the proliferation of fibrobl asts from healthy persons(NM-FB) and patients with oral submucous fibrosis(OSF -FB).Methods: Fibroblast cultures were established from t he buccal mucosa of five healthy individuals and six patients with OSF. The effe cts of TNF-? and IFN-? on the proliferation of NM-FB and OSF-FB were studi ed by MTT colorimetric assay.Results: TNF-? at 100～10 0 00 U/ml stimulated NM-FB and OSF-FB proliferation (P

OBJECTIVETo analyze caries activity of nasopharyngeal carcinoma (NPC) patients before and after radiation therapy. The study was also designed to analyze calcium and phosphorus concentrations in saliva of NPC patients before and after radiotherapy.

METHODSTwenty-eight NPC patients and 20 healthy controls were included in this study. Resazurin disc method was used to detect caries activity of NPC patients before and after 70 Gy radiotherapy. The atomic absorption spectrometry and molybdenum-stibium antispetrophotography method were used to analyze calcium and phosphorus concentrations in saliva of NPC patients before and after 70 Gy radiotherapy and the healthy controls.

RESULTSThe results of resazurin disc method showed caries activity of NPC patients was increased after radiotherapy (P < 0.01). Calcium concentration in saliva of NPC patients before radiotherapy was (63.19 +/- 3.27) mg x L(-1), phosphorus concentration was (132.96 +/- 5.13) mg x L(-1). After 70 Gy radiotherapy, calcium concentration was (33.38 +/- 0.32) mg x L(-1), phosphorus concentration was (49.18 +/- 2.66) mg x L(-1). The difference of calcium and phosphorus concentrations in saliva before and after radiotherapy was significant (P < 0.01).

CONCLUSIONCaries activities of NPC patients increase after radiotherapy. Calcium and phosphorus concentrations in saliva decrease after radiation therapy.

OBJECTIVE: To investigate dental caries of deciduous teeth among preschool children of Kaifu district in Changsha. METHODS: According to the criteria recommended by the Third National Oral Health Investigation, deciduous caries of 459 children aged 2 to 4 were clinically examined. The mean decayed missing filled teeth (dmft) and the prevalence of dental caries for each age group were calculated. The Results were statistically analyzed using SPSS13.0 software package. RESULTS The prevalence of dental caries was 39.65%.The mean dmft score was 1.32. The prevalence of dental caries and mean dmft increased as children grew up (P<0.05).Dental caries of maxillary deciduous central incisor and mandibular second deciduous molar were significantly more common than other teeth. The filling rate was 7.57%, which increased with age. The prevalence of early childhood caries was 17.21% (17.90% for males and 16.34% for females). CONCLUSION Deciduous dental caries of preschool children are an important problem, and the filling rate is very low. Necessary measures for early prevention of dental caries must be taken.
Child, Preschool ; China ; epidemiology ; Dental Caries ; epidemiology ; prevention & control ; Female ; Humans ; Male ; Prevalence ; Tooth, Deciduous

OBJECTIVE: To establish a suitable environment for the bioengineered teeth in vivo by observing the dentification ability of BALB/C mice tooth germs homologically implanted into the oral submucosa. METHODS: The first molar tooth germs of BALB/C mice 4 days after birth were transplanted into the oral submucosa of BALB/C male mice, and then recycled for regular histological observation after 1, 2, 3, and 6 week transplantation. RESULTS: The tooth germs in the oral submucosa grew well with continuing developing enamelum and pulpodentinal complex, and the dentinal tubules were clear. CONCLUSION The environment of the BALB/C male mice oral submucosa is favorable for the growth of tooth germs in inbred strain BALB/C mice, and it can provide a new environment for the development of bioengineered teeth in vivo.
Animals ; Animals, Newborn ; Dentinogenesis ; physiology ; Male ; Mice ; Mice, Inbred BALB C ; Molar ; transplantation ; Mouth Mucosa ; surgery ; Tissue Engineering ; Tooth Germ ; transplantation

In order to strengthen the stomatology experiment teaching,mehods such as constructing the stomatology experimental teaching platform,improving teaching methods and reforming the system of exam and assessment were adopted.Through these measures,opration and the innovative ideology of students were obviousely enhanced,students' interest were estimulated,the innovative spirit was enlightened and the comprehensive potential ability was developed.

OBJECTIVE: To observe the therapeutic effect of salvia miltiorrhiza and prednisolone on patients with oral submucous fibrosis (OSF). METHODS: A total of 60 medium-term OSF patients and 60 advanced stage OSF patients were randomly divided into the first group (treated with both salvia miltiorrhiza and prednisolone) and the second group (treated with prednisolone alone). The clinical effect was compared between each group after 3-month treatment. RESULTS: Difference was found in the lesion area of the medium-term cases and the advanced stage cases of the first group before the treatment [(10.37+/-3.40) cm2, (19.60+/-3.27) cm2] and after the treatment [(5.90+/-4.10) cm2, (16.33+/-4.02) cm2] (P<0.05). Significant difference was found in the mouth opening before the treatment [(3.41+/-0.77) cm, (1.98+/-0.39) cm] and after the treatment [(3.87+/-0.67) cm, (2.26+/-0.46) cm] (P<0.05) in the first group. There was significant difference in the lesion area and mouth opening of the medium-term cases of the second group before the treatment [(10.87+/-3.18) cm2, (3.57+/-0.75) cm] and after the treatment [(6.70+/-3.75) cm2, (3.97+/-0.69) cm] (P<0.05). No difference in the lesion area and mouth opening of the advanced stage cases of the second group was found (P>0.05). There was difference in the therapeutic efficacy between the first group (70%) and the second group (16.67%) of the advanced stage cases (P<0.05), but not in the clinical effect between the 2 groups of the medium-term cases (P>0.05). The side effect of prednisolone could be reduced while used together with salvia miltiorrhiza. CONCLUSION There is obvious advantage in treating OSF by the combination of salvia miltiorrhiza and prednisolone.
Adult ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Male ; Middle Aged ; Oral Submucous Fibrosis ; drug therapy ; Phytotherapy ; Prednisone ; therapeutic use ; Salvia miltiorrhiza ; chemistry

OBJECTIVE: To observe the expression and distribution of transforming growth factor β3 (TGF-β3) in the mouse tooth germ after advanced bell stage, and to discuss the role of TGF-β3 during the development of tooth germs. METHODS: BALB/C's mouse tooth germs at 4, 11, and 18 days postnatal (4dpn,11dpn,and 18dpn) were collected and processed for routine fixation, decalcification, embedding, and slicing. The expression of TGF-β3 was detected by immunohistochemisty. RESULTS: As to 4dpn tooth germ: Positive expression of TGF-β3 was found in enameloblasts, odontoblasts, ambitus of dental pupilla, with weak positive expression in the intermedial of dental papilla. As to 11dpn tooth germ: Positive expression was seen in enameloblasts, with negative expression in odontoblasts and dental papilla. As to 18dpn tooth, positive expression of TGF-β3 was showed in the vessel wall and its surrounding, with negative expression in other areas. CONCLUSION The distribution of TGF-β3 expression showed a time-space characteristic during the mouse tooth germ development after advanced bell stage, which may exert a regulatory effect on tooth development and this effect is gradually getting weak with the development of tooth germs.
Animals ; Animals, Newborn ; Immunohistochemistry ; Mice ; Mice, Inbred BALB C ; Molar ; metabolism ; Tissue Distribution ; Tooth Germ ; growth & development ; metabolism ; Transforming Growth Factor beta3 ; metabolism

The 16S rRNA gene is the most commonly used molecular marker for identifying microorganisms. It is used in sequencing technology, including the first-generation, the second-generation, and the third-generation sequencing technology. A large number of studies on the 16S rRNA gene have contributed to a deeper understanding of oral microbial diversity. In the healthy oral cavity, there is microbial diversity in time and space. With the occurrence or development of oral diseases such as caries, periodontal disease, or halitosis, the microbial diversity will be changed.
High-Throughput Nucleotide Sequencing ; Mouth ; RNA, Ribosomal, 16S ; genetics

Objective: To explore the effects of Er: YAG lasers with different pulse energies and of Er: YAG lasers combined with Duraphat on dentine surface morphology and the dentinal tubule exposure rate, thereby providing a theoretical basis and parameters for clinically treating dentine hypersensitivity with Er: YAG lasers. Methods : Dentinal tubule exposure models were divided into five groups, namely, group A (single Er: YAG laser exposure), group B (Duraphat+ Er: YAG laser), and group C (Er: YAG laser+Duraphat), which were exposed to 6 levels of pulse energy (80 mJ/P- 250 mJ/P), and experimental control group D (Duraphat group) and blank control group E. Scanning electron microscopy was used to observe dentine surface morphology and dentinal tubule closure in these groups, and Image-Pro Plus 6.0 software was used for image analysis to measure the exposure rates of the five groups of dentinal tubules. Results: The dentinal tubule exposure rates for the A-D groups were significantly lower than that of the E group (P < 0.001); when the Er: YAG laser pulse energy was less than 250 mJ/P, the dentinal tubule exposure rates for the A-C groups were all lower than that of the D group, but when the pulse energy was higher than 250 mJ/P, the dentinal tubule exposure rates for the A-C groups were higher than that of the D group, showing significant differences (P < 0.001); under the same handling method, when the pulse energy was 80~200 mJ/P, the dentinal tubule exposure rate decreased as the pulse energy increased. If the pulse energy reached 250 mJ/P, the exposure rate of the dentinal tubules increased instead. There were apparently no significant differences between 80 mJ/P, 100 mJ/P, 160 mJ/P and 200 mJ/P in the A and C groups or between 80 mJ/P and 100 mJ/P in the B group (P > 0.05), while significant differences existed among the other groups (P< 0.05). Under the same pulse energy, the dentinal tubule exposure rates were A group > C group >B group; under different pulse energies, there existed significant differences between the A and B groups (P < 0.05); when the pulse energy was 120~250 mJ/P, there were significant differences between the B and C groups (P < 0.05), while there were no significant differences among other groups (P＜0.05). Conclusion Er: YAG lasers can close the dentinal tubules and play a cooperative role if combined with Duraphat; the effects of laser exposure are better if fluorine is applied beforehand; and dentinal tubule closure is positively correlated with the pulse energy of Er: YAG lasers within a certain energy scope, but when the pulse energy reaches 200 mJ/P, the dentine surface is excessively fused and injured.

OBJECTIVE: To explore the association of the dental decay of children with the contents of chemokine CCL28 and secretory immunoglobulin A (sIgA) in saliva. METHODS: A total of 108 children in 2 kindergartens of Changsha, with age from 3 to 5 years old, were enrolled for this study. The saliva was collected from these children when they were in the examination of mouth. Th e children were divided into 3 groups: A non-caries group [dynamical mean-field theory (DMFT)=0], a low caries group (DMFT=1-4) and a high caries group (DMFT ≥ 5). Th e contents of CCL28 and sIgA were measured by ELISA. RESULTS: The contents of CCL28 and sIgA in saliva were (121.22 ± 32.63) pg/mL and (16.49 ± 8.02) μg/mL, respectively. A positive linear correlation was found between the CCL28 content and sIgA content in saliva (r=0.734). Th e CCL28 and sIgA contents in saliva were positively correlated with the degree of dental caries in children (P<0.05). CONCLUSION The dental decay of children leads to the secretion of chemokine CCL28, which promotes the secretion of sIgA in saliva.
Chemokines, CC ; analysis ; Child, Preschool ; Dental Caries ; pathology ; Enzyme-Linked Immunosorbent Assay ; Humans ; Immunoglobulin A, Secretory ; analysis ; Saliva ; chemistry