1.Expression of the estrogen receptor α and β in mouse thymus
Guoning GUO ; Guoyan ZHU ; Yongjun SHANG ; Liang WEN ; Shiwei XU
Chinese Journal of Immunology 2010;26(4):291-293
Objective: To detect estrogen receptor α and β(ER-α, ER-β)protein expression in different age of mouse thymus.Methods:Protein expression of ER-α and ER-β in thymus was analyzed via immunohistochemistry.Moreover,the relationship between ER-α and cytokeratin 18(epithelial cell marker)was further tested through fluorescence double-staining.Results: Immunohistochemical analysis revealed that both ER-α and β protein was found in nuclei of some thymocytes of 3 month mice.However,expression of ER-β was absence while ER-α was still positive in aged mice, such as 12 months and 16 months old.Double staining further confirmed that lots of ER-α/β positive cells were Foxp3 negative cells.Conclusion: Expression of ER-β is absent while ER-α is still positive in thymus of aged mice, which indicates ER-α is the critical estrogen receptor that involves in thymic involution.Moreover, ER-α/β do not participate in Treg development within thymus.
2.A study of serum endotoxin and its gene expression in a model of cerebrogenic multiple organ dysfunction syndrome
Linping LIANG ; Chuanqiang QU ; Hongzhi GUO ; Lei WANG ; Guoyan WANG
Chinese Journal of Geriatrics 2000;0(04):-
Objective To study changes of serum endotoxin and its receptor CD14 gene expression in lung, liver, intestines, kidney tissues in model of acute forebrain ischemia complicated by multiple organ dysfunction syndrome(MODS), and to investigate the pathogenesis of cerebrogenic MODS. Methods 54 Wistar rats were randomly divided into seven groups: normal control group(n=6)、sham-operative group(n=8) and 5 ischemia groups(n=40)including 12 h, 24 h, 36 h, 48 h, 72 h five time points. Model of cerebral infarction was established. The content of endotoxin in plasma was evaluated with a test kit. The area density and optical density of positive staining of CD14mRNA expressing were analyzed for the relative content of CD14mRNA using in-situ hybridization and CMIA medical imaging analysis system. Results Plasma endotoxin level were markedly high at 12 h (0.184?0.055)Eu/L after acute forebrain ischemia, peaking at 24 h (0.639?0.064)Eu/L and it was somewhat decreased at 72 (0.117?0.024)Eu/L. The CD14mRNA expression in lung, liver, intestines, kidney tissues increased after brain ischemia, reaching the peak at the 24~36 h, and decreased after 48 h. The highest change of CD14mRNA expression was found in lung(P
3.Expression of the estrogen receptor ? and ? in mouse thymus
Guoning GUO ; Guoyan ZHU ; Yongjun SHANG ; Liang WEN ; Shiwei XU
Chinese Journal of Immunology 1986;0(04):-
Objective:To detect estrogen receptor ? and ?(ER-?,ER-?) protein expression in different age of mouse thymus.Methods:Protein expression of ER-? and ER-? in thymus was analyzed via immunohistochemistry.Moreover,the relationship between ER-? and cytokeratin 18(epithelial cell marker) was further tested through fluorescence double-staining.Results:Immunohistochemical analysis revealed that both ER-? and ? protein was found in nuclei of some thymocytes of 3 month mice.However,expression of ER-? was absence while ER-? was still positive in aged mice,such as 12 months and 16 months old.Double staining further confirmed that lots of ER-?/? positive cells were Foxp3 negative cells.Conclusion:Expression of ER-? is absent while ER-? is still positive in thymus of aged mice,which indicates ER-? is the critical estrogen receptor that involves in thymic involution.Moreover,ER-?/? do not participate in Treg development within thymus.
4.Distribution of high-intensity zone of lumbar intervertebral disc in patients with low back and/ or leg pain and its related factors
Xian HE ; Anjing LIANG ; Yan PENG ; Wenjie GAO ; Guoyan LIANG ; Dongsheng HUANG
Chinese Journal of Postgraduates of Medicine 2012;35(11):1-4
ObjectiveTo investigate the distribution of high-intensity zone (HIZ) of lumbar intervertebral disc in patients with low back and/or leg pain,and analyze its related factors.MethodsSix hundred and twenty-eight patients with low back and/or leg pain were examined by MRI scan from June 2009 to August 2010.According to the diagnostic criteria of HIZ,the features of distribution of HIZ on age,segment and degree of intervertebral disc degeneration were analyzed retrospectively.ResultsAmong 3140 intervertebral discs of the 628 patients,172 cases (27.39%,172/628) and 206 discs (6.56%,206/3140)were involved with HIZ.There was no significant difference between men and women [26.38%(86/326)vs.28.48%(86/302)] (P=0.556).HIZ occurred more often [40.22%(72/179)] in those patients between 40 and 49 years of age.The incidence of HIZ at the segments from L1-2,L2-3,L3-4,L4-5,L5-S1 was 0.80%(5/628),2.07%(13/628),2.07%(13/628),14.01%(88/628) and 13.85%(87/628) respectively.In cases with and without HIZ,the incidence of intervertebral disc degeneration up to grade V was 49.03%(101/206) and 23.76%(697/2934) respectively (P < 0.01 ).HIZ was correlated with age,degree of intervertebral disc degeneration and disc segment (r =-0.040,P=0.025 ;r =0.217,P< 0.01 ;r =0.179,P< 0.01 ).Conclusions HIZ is correlated with age,degree of intervertebral disc degeneration and disc segment.Intervertebral disc degeneration plays the most important role in the occurence of HIZ.HIZ_mainly occur in L4-5 and L5-S1 segment and in those between 40 and 49 years of age.
5.Treatment of delayed deep infection after spinal fixation
Dong YIN ; Xiaoqing ZHENG ; Honglin GU ; Yongxiong HUANG ; Jianxiong ZHUANG ; Guoyan LIANG ; Shuaihao HUANG ; Changxiang LIANG ; Yunbing CHANG
Chinese Journal of Orthopaedics 2017;37(18):1150-1155
Objective To explore the efficacy of early aggressive debridement with implant retention, primary wound clo-sure, closed suction drain without irrigation and antibiotic therapy for the treatment of delayed deep infection after spinal fixation. Methods 4057 patients were underwent dorsal spinal fixation from January 2010 to June 2014. Among them, 42 cases of de-layed deep infection after operation were included in the study. There were 25 males and 17 females, with an average age of 68.6± 8.1 years (ranged from 53 to 83 years). The diagnosis of delayed deep infection was based on the time of onset, clinical symptoms and signs, imaging and laboratory findings. Surgical debridement was performed immediately after diagnosis of infection. In addi-tion, devitalized and necrotic tissue and biofilms which adhered to the surface of the implant were removed meticulously and thor-oughly. Primary wound closure was performed in each patient, and closed suction drains were maintained for about 7-10 d without irrigation. Routine sensitive antimicrobial drugs was applied for 3 months after operation. Results 42 cases were all followed up for 24 to 72 months with an average of 46 months. Among the 42 infected patients, 3 patients were underwent posterior cervical spine surgery and 39 patients were underwent posterior lumbar spine surgery. There were 13 cases of staphylococcus aureus infec-tion, 7 cases of escherichia coli infection, 3 cases of ESBL escherichia coli infection, 3 cases of enterobacter cloacae infection, 2 cases of MRSA, 2 cases of acinetobacter baumannii infection, 2 cases of klebsiella pneumoniae infection, 1 case in enterococcus faecium and pseudomonas aeruginosa and staphylococcus haemolyticus, respectively. There were still 7 patients with negative bacterial culture. 41 cases retained their implant, whereas 1 staphylococcus aureus infection patient had the implants removed be-cause of loosening during debridement. Nevertheless, primary wound healing was found in all patients, and stitches were removed 2 to 3 weeks after debridement. Infections were effectively controlled with no recurrence of infection during the follow-up. The av-erage erythrocyte sedimentation rate was (65.76±20.08) mm/h preoperative, (41.43±14.65) mm/h 1 month postoperative, (10.81±2.72) mm/h 6 months postoperative, and (8.10±5.46) mm/h 12 months postoperative, respectively, the differences were statistically significant. The average C reactive protein was (40.55±16.91) mg/L preoperative, (6.50±2.46) mg/L 1 month postoperative, (4.31± 1.26) mg/L 6 months postoperative, and (3.83±1.50) mg/L 12 months postoperative, respectively, the differences were statistically significant. The average procalcitonin was (0.47±0.28) ng/ml preoperative, (0.08±0.06) ng/ml 1 month postoperative, (0.06±0.03) ng/ml 6 months postoperative, and (0.05±0.00) ng/ml 12 months postoperative, respectively, and the differences were statistically significant. Conclusion A timely diagnosis, aggressive and meticulous debridement, high vacuum closed-suction drain, routine and adequate use of antibacterial agents are keys to successfully resolving infection and maintaining implant retention in the treat-ment of delayed deep infection after spinal fixation.
6.Treatment of cervical ossification of the posterior longitudinal ligament by modified laminoplasty with C3 laminectomy and C7 spinous process retention
Changxiang LIANG ; Guoyan LIANG ; Dan XIAO ; Shuaihao HUANG ; Yuhong KE ; Yunbing CHANG
Chinese Journal of Orthopaedics 2018;38(24):1502-1510
Objective To observe the clinical efficacy of modified laminoplasty with C3 laminectomy and C7 spinous process retention in the treatment of cervical ossification of the posterior longitudinal ligament(OPLL).Methods From February 2014 to March 2016,133 cases of OPLL were admitted,and 42 OPLLs were included according to the inclusion and exclusion criteria.A case-control study was conducted.Twenty-two cases were included in modified group and 20 cases in traditional group.There were 13 males and 9 females in the modified surgery group,and the average age was 56.2±9.75 years (39-77 years).Modified surgery was conducted with C3 laminectomy,C4-C6 laminoplasty,resection at the upper part of the C7 lamina and retaining the C7 spinous process.Meanwhile,there were 12 males and 8 females in the traditional surgery group,with an average age of 53.7± 8.23 years (41-75 years).Patients in the traditional surgery group were treated with standard C3-C7 laminoplasty.The mean followup was 22.9±4.5 (15-29) months.The JOA,NDI scores of the two groups were recorded preoperatively and postoperatively.The physiological curvature and activity of the cervical spine before and after operation were observed,and the incidence of axonal symptoms and postoperative complications in both groups were recorded.Results The follow-up period was 15-28 months,with an average of (23.2 ± 4.8) months.There was no significant difference between the base line of two groups before operation.At the last follow-up,the JOA and NDI scores of the two groups were significantly improved.There was no difference of JOA scores between the two groups at the last follow-up,while the NDI sores at the modified group(6.56±4.78) was superior to the traditional group(9.25±7.63).The VAS score at the first day after surgery in modified group was lower than that of the traditional group.The average cervical curvature of the patients in the modified surgery group and the standard surgery group were 12.32°±8.26° and 11.56°± 8.05°,respectively.There was no significant difference between the two groups.The postoperative range of movement (ROM) of cervical spine was 39.68°±9.52° in modified group and 33.51°± 10.39° in traditional group(P<0.05).Eight patients (19%) had augmentation of axial symptoms,including 3 patients in the modified surgery group(13.6%) and 5 patients in the standard surgery group (30%).There was no significant difference between the two groups.During the follow-up period,there were no complications such as cerebrospinal fluid leakage,spinal cord injury,wound infection,laminar collapse,postoperative closure,internal fixation loss or fracture.Conclusion The modified laminectomy with C3 laminectomy and C7 spinous process retentionis minimally invasive,whichachieved satisfactory decompression effect,reduced the incidence of postoperative axial symptoms and maintained the cervical ROM.
7.Quantitative CT for predicting efficacy of medical thoracoscopic giant emphysematous bullae volume reduction
Tingting LIANG ; Yaohua YU ; Zhenhua LI ; Guoyan LI ; Xinna ZHANG ; Hua ZHANG
Chinese Journal of Medical Imaging Technology 2023;39(12):1819-1822
Objective To observe the value of quantitative CT(QCT)for predicting the efficacy of medical thoracoscopic giant emphysematous bullae(GEB)volume reduction.Methods Data of chest QCT and pulmonary function tests before and 6 months after medical thoracoscopic volume reduction in 26 chronic obstructive pulmonary disease(COPD)patients complicated with GEB who underwent medical thoracoscopic GEB volume reduction were retrospectively analyzed.According to the improvement rate of lung function(forced expiratory volume in one second[FEV1])6 months after treatment compared with that before treatment,the patients were divided into significant improvement group(FEV1 improvement rate≥15%,n=20)and non-significant improvement group(FEV1 improvement rate<15%,n=6).QCT parameters before treatment were compared between groups,and the correlations of QCT parameters being significantly different between groups with FEV1 improvement 6 months after treatment were analyzed.Then receiver operating characteristic(ROC)curves were drawn to evaluate the efficacy of the above parameters for predicting the curative effect of medical thoracoscopic volume reduction of GEB.Results Significant differences of preoperative target lung lobe volume,total lung volume and bullae volume were found between groups(all P<0.05).Preoperative target lung lobe volume,total lung volume,target lobar bullae volume and total lung bullae volume were positively correlated with FEV1 improvement(r/rs=0.600,0.470,0.699,0.523,all P<0.05),with the area under the curve(AUC)for predicting FEV1 improvement of 0.817,0.817,0.892 and 0.833(all P<0.05),respectively.Conclusion QCT could effectively predict the efficacy of medical thoracoscopic GEB volume reduction.
8.Blood consumption and clinical prognosis in patients with massive blood transfusion: a retrospective analysis
Guilong LIU ; Dandan LI ; Yiling LIU ; Guoyan LIANG ; Guodong HUANG
Chinese Journal of Blood Transfusion 2023;36(2):148-152
【Objective】 To investigate the incidence of clinical massive blood transfusion in hospitals, the proportion of departments conducted massive blood transfusion and the current situation of component transfusion, so as to provide a theoretical basis for medical decision-making and further research on massive blood transfusion. 【Methods】 The basic clinical data and transfusion of blood components were retrospectively collected from 489 patients (514 occasions) who received massive blood transfusion at Sun Yat-sen Memorial Hospital of Sun Yat-sen University from Jan. 1 2014 to Dec. 31 2018. 【Results】 The incidence of massive blood transfusion during the 5-year period was 1.2/1 000 inpatients (95%CI: 1.1-1.3), and the 30-day all-cause mortality was 21.88%; in the departments where massive blood transfusion occurred, the mortality rate was the highest in the trauma emergency department (60%), followed by intensive care unit (56.25%) and other surgery department (46.67%), while there was no death in the obstetric department. All patients received red blood cells [median 14 U (11.5-19.13)] and plasma [median 1 600 mL (1 200-2 200)], of which 47% received platelet [median 0 U (0-10)] and 32.68% received cryoprecipitate [0 U (0-10)]. The results of logistics regression analysis of all-cause mortality risk showed that compared with the youth group, the risk of all-cause death at 30 days of elderly patients over 65 years old (65 < age ≤79 years old: OR=2.471, 95%CI=[1.044, 5.847], P<0.05; patients with age > 80 years old: OR=7.563, 95%CI=[1.587, 36.049], P<0.05) and 24-hour RBC infusion volume greater than 18 U (18≤RBC<27: OR=2.948 95%CI=[1.592, 5.462], P<0.05; RBC≥28: OR=3.992, 95%CI=[1.178, 13.536], P<0.05) was higher. 【Conclusion】 A dynamic definition should be included in massive transfusion studies. If only a 24-hour RBC infusion volume ≥18 U was used as the mass transfusion definition, about 68% of cases would be lost. The mortality rate of patients with massive blood transfusion was higher, and the incidence of massive blood transfusion was higher in the departments of cardiac surgery, general surgery and orthopedics surgery. More attention should be paid to the increasing number of female patients with massive blood transfusion. In addition, the risk of 30-day all-cause death was highest in elderly patients over 65 years of age and those with a 24-hour erythrocyte transfusion level of ≥18 U.