Objective To establish the reference interval of 3-14 years old children in Nanhai district of Foshan city .Methods 2 032 cases of 3-14 years old healthy children were detected and analyzed using MINDRAY BC-5800 automatic blood cell analy-zer .The levels of WBC ,RBC ,Hb ,PLT ,Hct ,MCV ,MCH ,MCHC ,RDW were analyzed .Results The levels of WBC ,RBC ,Hb , PLT ,Hct ,MCV ,MCH ,MCHC ,RDW in 2 031 cases were non normal distribution .The levels of Hb ,Hct had no statistical signifi-cance between male children and female children (P>0 .05);The levels of WBC ,RBC ,PLT ,MCV ,MCH ,MCHC ,RDW had statis-tical significance between male children and female children (P<0 .05) .Conclusion It is very important to establish the reference interval of 3-14 years old children in Nanhai district of Foshan city .

Objective Detection of single nucleutide polymorphisms in CD31-563 codon by denaturing high-performance liquid chromatography(DHPLC). Methods The eighth exon fragments of CD31 code gene in chromosome 17q23 were amplified by PCR. The fragments were analysed with DHPLC and were sequenced and compared with the sequences available in National Center for Biotechnology Information ( NCBI) database. Results The length of the amplified fragments is 203bp. There are three kinds of pictures when the fragments are analysed by DHPLC, after comparing with direct sequencing, the pictures of G/G homozygous、A/A homozygous、G/A heterozygous are obviously different, thus the individuals with the three kinds of genetype can be distinguished accurately. In 74 healthy people studied,the gene frequencies of CD31-563S(AGC) is 0.514, the gene frequencies of CD31-563N( AAC) is 0. 486. Conclusion DHPLC can effectively, economically and accurately detect the single nucleutide polymorphisms in CD31 -563 codon.

Objective To investigate the diagnostic value of serum human epididymis protein 4 (HE4 ) for ovarian cancer . Methods Electrochemiluminescence immunoassay was employed to detect the serum HE 4 of 91 patients with ovarian cancer (ovari-an cancer group) ,69 patients with benign ovarian disease(benign group) and 60 healthy people(control group) .Area under the curve(AUC ) of receiver operator characteristic (ROC ) curve for HE4 was calculated .Results Serum HE4 level [(146 .81 ± 81 .29)pmol/L] of patients in ovarian cancer group was significantly higher than those in control group [(43 .16 ± 25 .64) pmol/L] and benign group[(59 .86 ± 39 .87) pmol/L](P<0 .01) .Set serum HE4≥140 pmol/L as positive cutoff value ,the diagnostic sensi-tivity of serum HE4 detection for ovarian cancer was 82 .4% (75/91) ,specificity 89 .9% (62/69) and diagnostic efficiency 85 .6% . Serum HE4 levels of patients with serous adenocarcinoma ,endometrial adenocarcinoma or other ovarian cancer were markedly high-er than that with mucinous adenocarcinoma (P<0 .01) .Serum HE4 levels of patients with stage Ⅲ ,Ⅳ ovarian cancer were obvious-ly higher than those with Ⅰ ,Ⅱ stage(P<0 .01) .Conclusion HE4 detection has clinical value for the auxiliary diagnosis of ovarian cancer .

OBJECTIVESTo explore the clinical and pathological characteristics of stage IV breast cancer and to analyze their relationship with the morbidity and prognosis.

METHODSThe records of 66 patients presenting from January 2008 to December 2014 with stage IV breast cancer were reviewed. All of the patients were women and the median age was 57.5 (31 to 80) years, accounted for 3.01% (66/2 189) among the breast cancer patients treated in the same period. Statistical methods were used to analyze the correlation between clinical and pathological characteristics such as T-stage, N-stage, immuno-histo-chemistry and the morbidity and prognosis of stage IV breast cancer. The influence of patients characteristics to metastasis were compared by χ(2) test. Kaplan-Meier curves were reported for overall survival (OS), and the Log-rank test was used to compare the difference in groups. Cox proportional models were fitted for multivariate analysis.

RESULTSThe median survival time of stage IV breast cancer was 56.0 months and the 5-year survival rate was 40%. To metastasis, the effects of age, subtypes, histological grade, hormone receptor (HR) and human epidermal growth factor receptor 2 (HER2)had no significant statistics differences. It was concluded that the expression of HER2 (P=0.003) and HR (P=0.001) as well as single metastasis (P=0.029) were the influencing factors of the survival by multivariate Cox regression analysis. Primary tumor R0 surgery group and no surgery group had no significant statistics differences of overall survival and the 5-year survival rate (P=0.102).

CONCLUSIONSClinical and pathological characteristics have no effect on metastasis. The expression of HER2 and HR as well as single metastasis play important roles in survival.

Adult ; Aged ; Aged, 80 and over ; Breast Neoplasms ; Female ; Humans ; Middle Aged ; Multivariate Analysis ; Neoplasm Staging ; Prognosis ; Proportional Hazards Models ; Receptor, ErbB-2 ; Survival Rate

Parry-Romberg syndrome (PRS) also known as progressive hemifacial atrophy, is a rare craniofacial disorder characterized by chronic hemifacial atrophy of the skin, subcutaneous tissue, fat, underlying muscle and bone. Early diagnosis and treatment of the disease are of great significance. This article will review the latest progress in the pathogenesis, early diagnosis and clinical treatment of the disease.

Parry-Romberg syndrome (PRS) also known as progressive hemifacial atrophy, is a rare craniofacial disorder characterized by chronic hemifacial atrophy of the skin, subcutaneous tissue, fat, underlying muscles and bone. Early diagnosis and treatment are of great significance. This article has summarized the latest progress in the pathogenesis, early diagnosis and clinical treatment of the disease.

Parry-Romberg syndrome (PRS) also known as progressive hemifacial atrophy, is a rare craniofacial disorder characterized by chronic hemifacial atrophy of the skin, subcutaneous tissue, fat, underlying muscle and bone. Early diagnosis and treatment of the disease are of great significance. This article will review the latest progress in the pathogenesis, early diagnosis and clinical treatment of the disease.

Parry-Romberg syndrome (PRS) also known as progressive hemifacial atrophy, is a rare craniofacial disorder characterized by chronic hemifacial atrophy of the skin, subcutaneous tissue, fat, underlying muscles and bone. Early diagnosis and treatment are of great significance. This article has summarized the latest progress in the pathogenesis, early diagnosis and clinical treatment of the disease.

Objective:To develop a nomogram model based on clinical and imaging parameters to predict the etiological type of acute ischemic stroke (AIS).Methods:Patients with AIS received endovascular treatment in Beijing Hospital from March 2016 to December 2021 were retrospectively included. According to the etiological type, they were divided into large artery atherosclerosis (LAA) and cardioembolism (CE). The clinical and imaging parameters mostly relevant to the etiological type were selected by LASSO regression, and a nomogram model for predicting the etiological type of AIS was established by multifactorial logistic regression to investigate the predictive value of relevant clinical imaging parameters. In addition, the diagnostic efficacy of the prediction model was assessed by receiver operator characteristic (ROC) curves, calibration curves, and clinical decision curves. Results:A total of 136 AIS patients with anterior circulation large vessel occlusion received endovascular treatment were included, including 62 patients with CE (45.6%) and 74 with LAA (54.4%). Variables with P<0.10 in the univariate analysis were included in LASSO regression to screen for relevant variables. The gender, baseline National Institute of Health Stroke Scale (NIHSS) score, penumbra to ischemic core ratio, brain natriuretic peptide (BNP), and platelet (PLT) count were included into the multivariate logistic regression model. The results revealed that gender (odds ratio [ OR] 2.632, 95% confidence interval [ CI] 1.048-6.607; P=0.039), baseline NIHSS score ( OR 1.078, 95% CI 1.002-1.160; P=0.043), BNP ( OR 1.004, 95% CI 1.002-1.007. P<0.001), PLT ( OR 0.991, 95% CI 0.982-0.999; P=0.031) as the predictors to distinguish LAA from CE. In addition, the penumbra to infarct core ratio ( OR 0.886, 95% CI 0.785-1.000; P=0.050) also played an important role in predicting the model. The diagnostic efficacy of this predictive model was analyzed by the ROC curves, with an area under the curve of 0.881 (95% CI 0.815-0.930, P<0.001). Bootstrap internal validation showed that the good compliance with a mean absolute error of 0.027 for true versus predicted value compliance. Calibration curves, clinical decision curves, and Hosmer-Lemeshow test ( P=0.562) showed good agreement between the predicted and actual values of the model. Conclusion:Patients with CE are more common in women, have higher NIHSS scores and BNP, and have lower PLT and penumbra to ischemic core ratio. The nomogram model combining the above indicators can better identify LAA and CE, and maybe helpful in clinical decision making.

Purpose To explore the application value of forward projected model-based iterative reconstruction solution(FIRST)algorithm improving image quality of 100 kV low-dose head and neck CT angiography(CTA).Materials and Methods A total of 32 patients who underwent head and neck CTA examinations in the Beijing Hospital from September to October 2021 were retrospectively recruited.All patients were divided into three groups based on different reconstruction,including the filtered back projection(FBP)group,the adaptive iterative dose reduction 3D(AIDR 3D)group,and the FIRST group.The average CT value,image noise,signal-to-noise ratio,contrast to noise ratio and other objective indicators of the main blood vessels images(including common carotid artery,vertebral artery,internal carotid artery C1,C4,middle cerebral artery M1,M3 and anterior cerebral artery A1,A3)and background(including muscle,brain tissue)in the head and neck of the three groups were recorded and analyzed,respectively.The subjective imaging evaluation was scored and analyzed.Digital subtraction angiography(DSA)was used as the gold standard to evaluate the detection of vascular stenosis in the head and neck by each reconstruction algorithm.Results Compared with the AIDR 3D group and the FBP group,the FIRST group significantly reduced the image noise of the blood vessels and the background(muscle,brain tissue)(t=-13.19--7.28,all P<0.001).The signal-to-noise ratio and contrast to noise ratio of the FIRST group were significantly higher than those of the FBP group,AIDR 3D group(t=2.17-9.67,all P<0.001).The CT values of FIRST group were significantly higher than those of AIDR 3D group in the common carotid artery,internal carotid artery C1 and C4,middle cerebral artery M1,and anterior cerebral artery A1 segment(t=1.28-3.60,all P<0.05).The CT value of background(muscle,brain tissue)in FIRST group was significantly lower than those in the FBP group and the AIDR 3D group,with statistically significant difference(t=-7.63--4.03,all P<0.001).The images of FIRST group and AIDR 3D group met the diagnostic requirements,and the subjective scores of the two groups were significantly higher than those of the FBP group images(all P<0.05).The imaging scores of FIRST group were significantly higher than those of AIDR 3D group(P<0.05).Compared with DSA,FBP was ineffective for mild and moderate stenosis;FIRST and AIDR 3D algorithms were consistent with DSA.Conclusion Compared with traditional FBP and AIDR 3D reconstruction algorithms,FIRST algorithm can effectively improve the image quality of low-dose head and neck CTA and obtain better image quality and meet the diagnostic performance of head and neck vascular diseases.