Objective To investigate the mRNA and protein expression of GPX3 gene in non-small cell lung cancer (NSCLC) patients, and then discuss the clinical significance of GPX3 in NSCLC patients. Methods Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were used to detect the GPX3 mRNA and protein levels in NSCLC tissues and adjacent non-cancerous normal lung tissues from 60 patients undergoing surgical treatment. The correlation between the expression levels of GPX3 and clinicopathological features was analyzed. Results The relative expression value of GPX3 mRNA was higher in adjacent non-cancerous tissues than in non-small cell lung cancer tissue (P < 0.05). GPX3 mRNA expression was significantly correlated with TNM stage、differentiation and lymph node metastasis. The integral optical density value of GPX3 protein confirmed was lower in adjacent non-cancerous tissues than in non-small cell lung cancer tissues (P < 0.05). In additional, the expression of GPX3 was related to TNM stage and lymph node metastasis. Conclusion The expression of GPX3 gene may play an important role in the carcinogenesis and progression of NSCLC.

Objective To study the function of vascular endothelial growth factor (VEGF) in type 2 diabetes model rats and its effect on focal cerebral ischemia induced by middle cerebral artery occlusion in these rats. Methods Focal cerebral ischemia was induced by middle cerebral artery occlusion for 6 hours in type 2 diabetes rats and normal control rats.Blood vessels morphology was examined by ink perfusion,infarct size was measured by TTC and expression of VEGF and CD34 were evaluated by immunohistochemistry staining. Results Ink perfusion revealed increased number of small vessels in type 2 diabetes rats. Infarct size was significantly smaller in type 2 diabetes rats ( ( 80. 07 ± 11.21 ) mm3 ) than that in normal controls ((98. 91 ± 14. 86) mm3,t = 2.48,P = 0. 0326). There were more hemorrhage lesions in the ischemic hemisphere in type 2 diabetes rats when comparing with the controls. VEGF and CD34 showed significantly higher expression in type 2 diabetes rats than in normal controls. Conclusions High expression of VEGF and CD34 are found in type 2 diabetes rats after middle cerebral artery occlusion. There is cerebrolvascular remodeling in diabetes rats. While this diabetes-induced remodeling appears to prevent infarct expansion,the changes also increase the risk of hemorrhagic transformation. The latter may result in poor prognosis.

Objective To investigate the changes of T lymphocyte subsets in patients with acute exacerbation of chronic obstructive pulmonary disease(AECOPD). To evaluate the clinical efficacy of Xuebijing injection in the treatment of AECOPD and its influence on T lymphocyte subsets. Methods From October 2016 to October 2017, 58 AECOPD patients and 20 healthy subjects at the same period in the First People's Hospital of Luoyang were selected. The levels of T - lymphocyte subsets were detected in healthy subjects and AECOPD patients. The 58 cases patients were divided into observation group(n = 28) and control group(n = 30) using random number table. The control group was treated with conventional therapy. The observation group was treated with Xuebijing injection on the basis of the control group. The scores of CAT,APACHE Ⅱ in both two groups before and after treatment were evaluatedThe levels of C - reactive protein(CRP),procalcitonin(PCT) and T lymphocyte subsets were compared between the two groups before and after treatment. Results The levels of CD +3 ,CD +4 and CD +4 / CD +8 in the peripheral blood of patients with AECOPD were (0. 64 ± 0. 10)％ ,(0. 33 ± 0. 14)％ and (0. 95 ± 0. 48),respectively,which of the healthy controls were (0. 70 ± 0. 10)％ , (0. 41 ± 0. 17)％ , (1. 25 ± 0. 51), respectively. There were statistically significant differences between the two groups(t = 2. 09,7. 19,2. 32,all P < 0. 05). After treatment,the scores of CAT and APACHE Ⅱ in the observation group were (13. 2 ± 3. 6)points and (14. 1 ± 3. 8)points,those in the control group were (16. 6 ± 4. 1) points and (16. 9 ± 4. 2) points. The scores of CAT and APACHE Ⅱ in the two groups after treatment were lower than those before treatment(observation group: t = 3. 08,13. 25,control group: t = 2. 94,11. 31,all P < 0. 05). There were significant differences in CAT and APACHE Ⅱ scores between the two groups after treat-ment(t = 8. 90,3. 27,all P < 0. 05). The levels of CRP,PCT,CD +3 ,CD +4 and CD +4 / CD +8 in the control group were (22. 4 ± 8. 8)mg/ L,(0. 9 ± 0. 6)μg/ L,(0. 67 ± 0. 09)％ ,(0. 36 ± 0. 13)％ ,(1. 12 ± 0. 50),respectively,which in the observation group were (15. 6 ± 6. 3)mg/ L,(0. 5 ± 0. 6)μg/ L,(0. 72 ± 0. 82)％ ,(0. 43 ± 0. 13)％ ,(1. 43 ± 0. 47),respectively. The differences between the two groups were statistically significant(t = 2. 75,6. 16,2. 03,2. 69, 4. 90,all P < 0. 05). Conclusion Patients with AECOPD have immune dysfunction. Xuebijing injection is effective in the treatment of patients with AECOPD. The mechanism may be related to the regulation of immune function.

Objective To investigate the value of endoscopic retrograde appendicitis therapy ( ERAT) in the diagnosis and treatment of atypical acute appendicitis. Methods All the 48 patients suspected of atypical acute appendicitis in Jiangsu Province Hospital from January 2015 to December 2016 were randomly divided into ERAT group and conservative treatment group according to the treatment method. The final appendectomy rate of the two groups was analyzed. Results Only 17 of the 24 patients in the ERAT group received endoscopic treatment because of complex conditions or personal wishes, and 16 cases were diagnosed as acute appendicitis. Surgical resection was performed in 5 cases because of recurrence of the disease after ERAT, and the appendectomy rate was 31. 2％ ( 5/16 ) . In the conservative treatment group, all 24 patients were treated with antibiotics. Twenty of them underwent surgical resection with appendectomy rate of 83. 3％ ( 20/24) , and 1 of them had appendiceal perforation. The appendectomy rate of the ERAT group was significantly lower than that of the conservative treatment group (χ2=11. 111, P<0. 05) . Conclusion ERAT has a high diagnostic and therapeutic value for atypical acute appendicitis.