AIM:To establish a digitized HPLC fingerprint of Compound Danshen Dropping PilLs (CDDP). METHODS:The RP-HPLC condition was optimized by the chromatographic fingerprint relative index (Fr). The chromatographic fingerprints were characteristically digitized and evaluated by the software of the digitized evaluation system of Traditional Chinese Medicine (TCM) with the super information characteristics. RESULTS:15 co-possessing peaks were selected as the fingerprint peaks of CDDP by taking protocatechualdehyde peak as the referential peak to establish the digitized fingerprint and the important digitized information for its quality control was obtained. The stabilities among different lots of samples were evaluated by the dual qualitative and dual quantitative similarity method. CONCLUSION:The fingerprints can perfectly reflect the authentical quality of CDDP.

Objective To investigate the mechanism of affecting smooth muscle cells apoptosis after balloon injury to vessel Methods The percentage of vascular smooth muscle cells (VSMC) apoptosis and the expression of angiotensin Ⅱ subtype 1 receptor (AT 1R) was measured by terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labeling (TUNEL) and immunohistochemical technique after balloon injury Results Compared with sham's, the expression of AT 1R protein in vascular media was significantly increased at 3 days after balloon injury ( P

AIM: To establish a novel method for the overall quality control of Fructus Gardeniae by HPLC digitized fingerprint and its normalization. METHODS: The chromatographic fingerprints were obtained by injecting 5 ?L of the sample solution each time on a Century SIL C_ 18 BDS column (20 cm?4.6 mm, 5 ?m) with the gradient elution solvent system composed of 1% acetate acid-water and 1% acetate acid- acetonitrile. The flow rate was 1.0 mL /min, the column temperature was maintained at (30?0.15)℃ and the detection wavelength was set at 265 nm. The chromatographic fingerprints were evaluated by the digitized parameters, such as the chromatographic fingerprint index (F), quantitative similarity, etc,. The influence of big peak on similarity was elaborated by direction cosines, and its cancellation was achieved by the qualitative similarity of peak area ratio. RESULTS: 35 co-possessing peaks were selected as the fingerprint peaks of Fructus Gardeniae by taking Geniposide peak as the reference peak. The HPLC digitized fingerprint and its normalization were implemented. CONCLUSION: This method with good precision and reproducibility can be useful in the quality control of Fructus Gardeniae.

AIM: To study the effects of ischemia/reperfusion (I/R) on primary cultured sinoatrial node (SAN) cells and the influence of pinacidil (a K_(ATP) channel activator). METHODS: The SAN cells were isolated from newborn rats and purified. The 48 h cultured cells were cultivated in following mediums: simulated reperfusion solution as normal control, simulated ischemia/reperfusion solution (I/R), Pinacidil+I/R (P+I/R), 5-HD+P+I/R and 5-HD+I/R. Spontaneous action potentials were recorded by ruptured-patch whole-cell technique in current clamp ((I=0)) and the maximum diastolic potential (MDP), upstroke velocity (UV), action potential overshoot (APO), interbeat interval (IBI) and action potential durations at 50% repolarization (APD_(50)) were measured. RESULTS: Compared with control group, simulated ischemia/reperfusion shorten APD_(50), reduced UV, MDP and APO. Exposed to pinacidil, MDP of cells in I/R groups was hyperpolarized; IBI, UV and APO were increased; APD_(50) was shorten. 5-HD couldn't block the effects of pinacdil on APD_(50), IBI and MDP, but reversed its actions on increasing UV and APO. CONCLUSIONS: Pinacidil made changes of AP in I/R group by opening different K_(ATP) channels of SAN cells. The role of this changes on protection in SAN cells during ischemia/reperfusion requires further investigation. [

AIM:A new method for the overall quality control of Radix Scutellariae has been established by HPLC digitized fingerprint. METHODS: The chromatographic fingerprints were obtained from injecting 5 ?L of the sample solution each time on a CenturySIL C_(18) BDS column(200 mm?4.6 mm,5 ?m) with the gradient elution solvent system composed of 1% acetic acid water and 2% acetic acid acetonitrile.The flow rate was 1 mL/min,the column temperature was maintained at(30?0.15)(?C) and the detection wavelength was set at 280 nm.The chromatographic fingerprints were evaluated by the software of the digitized evaluation system of Traditional Chinese Medicine fingerprint with the super information characteristics. RESULTS: 30 co-possessing peaks were selected as the fingerprint peaks of Radix Scutellariae and the similarities between the chromatographic fingerprints and the herbal drugs were calculated by taking baicalin peak as the referential peak.The chromatographic fingerprints were also evaluated by the chromatographic fingerprint index(F). CONCLUSION: This method with good precision and reproducibility can be applied to the quality control of Radix Scutellariae.

B-ultrasonography and/or CT scannings of 84 cases of enlarged superior ophthalmic vein (SOV) were analysed morphologically and etiologically. Carotid-cavernous fistula, ophthalmic Graves disease, Tolosa-Hunt syndrome, orbital vascular deformity, inflammation at the apex of the orbit, orbital pseudo tumor, intraorbital hematoma and thrombosis of the SOV might induce enlargement of SOV. Associated with other Findings of intraorbital tissues revealed by CT and B-ultrasonography, the etiological diagnosis of the enlarged SOV could be made. The dynamic findings of the SOV rendered by B-ultrasonography and Doppler ultrasonic play an important role in the diagnosis of carotid-cavernous fistula.

AIM:To establish the normalized chromatographic fingerprints(NCFP) of Shegan Antiviral Injectionl(Rhizoma Belamcandae,Flos Lonicerae japonicae,Herba Artemisiae scopariae,Radix Bupleuri,etc.)(SGKBDI) and to evaluate them by using the 25 relatively normalized parameters.METHODS:The chromatographic fingerprints of the eight herbal drugs,two intermediates and preparation of SGI were determined by RP-HPLC.The 11 chromatographic fingerprints were evaluated by 25 relatively normalized index calculated by the software of Digitalized Evaluation System of Super-information Characteristic for Traditional Chinese Medicine Fingerprints 3.0.RESULTS:The best fingerprints were S4 Herba Artemislae Scopariae and S7 Radix Isatidis,the next were S2 Flos Lonicerae、 S5 Radix Bupleuri、 S6 Herba Taraxaci、 S8 Folium Isatidis,S10 intermediat Ⅱ and S11 Sgkbdi.CONCLUSION:The 25 characteristic parameters can be used to objectively,authentically and thoroughly reveal the separation of chromatographic fingerprints and its normalized characteristics of the NCFP of SGKBDI.

50% and types 2 and 3 of plaque morphology) and mild lesion group (MG, narrowness of luminal diameter

Objective To compare the effect of enteral nutrition liquid with different dietary fiber content on serum protein in patients with stress hyperglycemia.Methods A total of 90 patients with stress hyperglycemia were randomly divided into three groups:enteral nutritional suspension (SP) group (Peptisorb),enteral nutritional suspension (TPF-FOS) group (Jevety) and enteral nutritional suspension (TPF-D) group (Glucerna),each group consisting of 30 patients.In the three groups,prealbumi (PA),serum albumin (ALB),retinol binding protein (RBP),transfer-rin (TRF) and hemoglobin (Hb) were continuously measured on the 1st,2nd,3rd,4th,5th,6th and 7th day.Data were analyzed by SPSS 15.0.Variances on time and group were analyzed by repeated measures of general linear model.Results The PA,ALB,RBP,TRF and Hb were significantly different among Peptisorb group,Jevety group and Glucerna group (P＜ 0.05).The PA,ALB and Hb which were determined in different time,were not significantly impacted by Peptisorb,Jevety or Glucerna,and not significantly changed with time.The RBP and TRF which were determined at different time,were not impacted by Peptisorb,Jevety or Glucema,but time× group from RBP and TRF which were determined in different time,were significantly impacted in Peptisorb group,Jevety group and Glucerna group (P＜ 0.01).Conclusion The content from different dietary fiber significantly affects serum protein in patients with stress hyperglycemia,and TPF-D has the most significant effect on serum protein.

Objective To evaluate the immediate and short term therapeutic efficacy of percutaneous transluminal septal myocardial ablation (PTSMA) in patients with hypertrophic obstructive cardiomyopathy (HOCM) and to investigate the prevention of the related complications. Methods PTSMA was conducted in 3 patients with HOCM refractory to medication. The immediate effect was evaluated by pressure monitor, but the follow up effect by observation of the clinical symptoms and ultrasonic cardiogram. Results The left ventricular outflow tract gradients triggered by ventricular premature beats decreased from 143 mmHg (ranging from 70 to 180 mmHg) to 53 mmHg (ranging from 30 to 80 mmHg). Complete atrioventricular block (Ⅲ?AVB) with atrioventricular node rhythm was found in 1 case, and complete right branch bundle block (CRBBB) in another one. Follow up of the 3 cases at 6 months after operation revealed remarkable improvement of the patients' cardiac function (NYHA), disappearance of angina pectoris, and obvious attenuation of ventricular septal hypertrophy and SAM (systolic anterior motion of mitral) phenomenon. Conclusion PTSMA is an effective method with satisfactory short term effect for the patients with HOCM refractory to medication.