Objective To study the value of imaging characters in diagnosing mediastinal tumors.Methods X-ray and CT findings of pathologically proved mediastinal tumors in 58 cases were analysed,the findings enabling qualitative diagnosis were recommended. Results There were 40 cases of anterior mediastinal tumors including intrathoracic thyroid tumors [n=32,5 tyroid carcinomas and 1 thyroid cyst ,thymomas (n=3) and teratomas (n=5, 3 cases ruptured)]. All the other 18 cases in posterior mediastinum were neurogenic tumors.Conclusion Based on the combination of the mediastinal compartment knowledge, X-ray and CT findings of the tumors and the clinical information, the qualitative diagnosis of mediastinal tumors will be characterized correctly.

Objective　To study the effect on the proliferation of vascular smooth muscle cells(VSMC) after transferring angiotensin Ⅱ(AngⅡ) type 2 receptor(AT2R) gene.Methods　The recombinant adenoviral vector AdcCMV-AT2R, containing rat AT2 receptor gene which was constracted by homologous recombination, was used to transfer AT2 receptor gene to rat VSMC in vitro. The expression of AT2R mRNA was detected by RT-PCR. The rate of expression and the change of cell cycle in VSMC were analysed by flow cytometry. Cell devision index, incorporation of bromodeoxyuridine(BrdU)and 3-(4,5-dimethyl-thiazol-2-yl)2,5-diphenytetrazolium bromide(MTT) were used to determine the proliferation of VSMC, respectively.Results　RT-PCR showed that the expression of AT2R mRNA increased obviously in transferred VSMC, and the peak value of expression rate was about 89.51% at 48 hours. When the expression of AT2R was at peak value, the ratio of S and G2-M periods was reduced from 31.7% to 13.9%(P<0.05) and the index of cell division from 37.4% to 9.6%(P<0.01). The OD values of MTT and BrdU incorporation were reduced by 61.4% and 51.6% respectively(P<0.01).Conclusion　Our study indicates that AdCMV-AT2R can generate high level expression of AT2 receptor in cultured rat VSMC and its expression can significantly inhibit the proliferation of rats VSMC in vitro. It may be a new selection for the gene therapy of restenosis after angioplasty.

Objective To investigate the mechanism of affecting smooth muscle cells apoptosis after balloon injury to vessel Methods The percentage of vascular smooth muscle cells (VSMC) apoptosis and the expression of angiotensin Ⅱ subtype 1 receptor (AT 1R) was measured by terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labeling (TUNEL) and immunohistochemical technique after balloon injury Results Compared with sham's, the expression of AT 1R protein in vascular media was significantly increased at 3 days after balloon injury ( P

Objective To evaluate the security and the reliability of ligating the cystic duct and the cystic ar- tery with common silk suture as the substitute for titanic or biological clamps in laparoscopic cholecystectomy (LC) us- ing the suture and knot instrument developed by the authors. Methods One hundred and two laparoscopic chole- cystectomies were performed by the authors using the instrument. Results All patients were discharged in the post- operative fourth or fifth day without any complication. Conclusion The instrument used for LC was safe and reliable.

Objective: To examine the developmental characteristics of the pupils' emotion display rules and its relationship to the peer acceptance. Methods: A sample of 157 pupils' emotion display rules and peer acceptance in third and fifth grades were tested by clinical interview and social nomination. Results: Third-graders compared to fifth-graders were more knowledgeable about expression regulation, and used more self-protective display rules. Girls were proposed more norm-maintenance display rules than boys. Urban children compared to country children were more knowledgeable about expression regulation, and used more prosocial display rules. Self-protective display rules can predict peer rejection. Conclusion: Pupils' emotion display rules have different developing characteristics, and can predict peer rejection to a certain extent. The development of display rules is beneficial to moderate children's expression regulation and social development.

Objective To construct the recombinant adenovirus of Angiotensin Ⅱ type 2 receptor (AT2R), and study the effect of AT2R on prevention of neointimal hyperplasia in rat carotid arteries after balloon angioplasty. Methods The recombinant adenovirus of AT2R with green fluorescent protein was constructed by using the method of homogenous recombination in bacteria. AT2R gene was transduced into rat carotid arteries with pAdCMV/AT2R after the reproduction of rat carotid balloon injury restenosis model. The expression and the transfection efficiency of AT2R gene were evaluated by RT-PCR, immunofluorescence staining, and confocal microscope. The intimal/medial area ratio was measured by digital analysis system. Results The titre of purified recombinant adenovirus was 1.5?10 12pfu/ml, and it was proved by PCR. pAdCMV/AT2R transfection efficiency in carotid artery was about 40% at day14, and localized to the neointima, as well as to the media and the adventitia. pAdCMV/AT2R delivered into injured rat carotid arteries significantly up-regulated AT2RmRNA and protein expression in neointima from day 7 to 21 after injury. Compared with pAd-GFP transfection group, pAdCMV/AT2R transfection reduced I/M ratio significantly on day 21(0.78?0.06 vs. 1.36?0.21 respectively, P

Objective Overexpressing gax gene in vascular smooth muscle cells(VSMC) mediated by adenovirus transfection.Methods Replication defective adenovirus type 5 (Ad5) vector encoding the rat gax gene (AdCMV gax) was constructed via site specific recombination and amplified in 293 cells. The resulting adenovirus was purified for a high viral titer. The expression of gax mRNA and protein was evaluated by a series of methods such as reverse transcription polymerase chain reaction (RT PCR), flow cytometry and immunocytochemical technique after gax gene was transferred to VSMCs. Results When AdCMV gax was transfected to VSMCs, the expression of gax protein increased significantly in VSMCs, and reached 80% in 24 hours. High expression of gax protein could keep for over 5 days. Before AdCMV gax was transfected to VSMCs, gax mRNA and protein could be down regulated by platelet derived growth factor (PDGF) BB. After AdCMV gax was transfected to VSMCs, the level of gax expression was significantly higher than that before transfection, and no correlation with the stimulation of PDGF BB.Conclusions gax gene can be transfected to VSMC by AdCMV gax and translated to protein, which is advantageous to exploring the effects of gax gene on biological behaviour of VSMC.

To study the expression of angiotensin Ⅱ (AngⅡ) type 2 receptor (AT2R) and its effect on the proliferation and migration of vascular smooth muscle cell (VSMC) in rats. The recombinant adenoviral vector, AdCMV AT2R, containing rat AT2 receptor gene was constructed by homologous recombination, and then it was used to transfer AT2 receptor gene to rat VSMC in vitro. The expression of AT2R, bcl 2 and bax mRNA was detected by RT PCR and the rate of expression in VSMC was determined by flow cytometer. Cell proliferation was determined by incorporation of bromodeoxyuridine(BrdU) and 3 (4,5 dimethyl thiazol 2 yl)2,5 diphenytetrazolium bromide(MTT) respectively. The modified Boyden chamber method was used to test the migration of VSMC. Apoptosis was quantified by flow cytometer and by terminal deoxynucleotide transferase mediated dUTP nick end labeling(TUNEL) in situ. RT PCR showed that the expression of AT2R mRNA increased obviously in transferred VSMC, and the peak value of expression rate was about 89.51% at 48 hours; bax mRNA was also increased by 76.3% at the same time. When the expression of AT2R was at peak value, the OD value of MTT and BrdU incorporation were reduced by 61.4% and 51.6% respectively( P

To investigate the changs in the expressions of cellular adhesion molecules (CAMs) of the myocardium after low dose interleukin 1? (IL 1?) pretreatment and the relationship between CAMs and delayed cardioprotection, we measured the change in the expressions of intercellular adhesion molecule 1 (ICAM 1) mRNA and its proteins, the expressions of leukocyte function antigen 1(CD11a) proteins, and infiltration number of polymorphonuclear leukocytes (PMNs) in the myocardium and determined the infarct size with in situ hybridization(ISH),immunohistochemistry and enzyme methods immediately,12 hours and 24 hours after IL 1? pretreatment in a model of ischemia/reperfusion of myocardium in rats. The results showed that the expressions of ICAM 1 mRNA and its protein, CD11a protein and the PMN infiltration number were significantly higher in the ILPC and NS groups than in the control at 0～24h after IL 1? or NS pretreatment( P 0 05). The results suggested that low dose IL 1? pretreatment might inhibit the expressions in the CAMs of myocardium during the late period of ischemia /reperfusion,resulting in delayed cardioprotection.

AIM:To evaluate the effects of atorvastatin on nitric oxide(NO),endothelin-1(ET-1)and myocardial no-reflow in a rabbit model of acute myocardial infarction and reperfusion(AMI/R). METHODS:Twenty-four rabbits were randomized into 3 groups:8 in AMI/R group,8 in atorvastatin-treated group(5 mg?kg-1?d-1)and 8 in sham-operated group. Animals in the former two groups were subjected to 60 min of coronary occlusion followed by 120 min of reperfusion. Data on haemodynamics were collected. NO in blood sample,and in normal,and in infarcted reflow and no-reflow myocardium were evaluated respectively by nitrate reductase method. The levels of ET-1 in blood sample,and in normal,infarcted reflow and no-reflow myocardium were determined by radioimmunoassay. RESULTS:(1)Compared to the baselines,the heart rate(HR),systolic blood pressure(SBP),diastolic blood pressure(DBP),left ventricular systolic pressure(LVSP),maximal rate of increase and decline in left ventricular pressure(?dp/dtmax)and cardiac output(CO)in AMI/R and atorvastatin-treated groups were significantly declined,whereas left ventricular end-diastolic pressure(LVEDP)was increased after 60 min of coronary occlusion and 120 min of reperfusion(P