Objective To investigate the detection rate of couples at high risk of bearing babies with severe thalassemia and the occurrence rate of birth defect caused by severe thalassemia among the people at reproductive age in Foshan city ,Guangdong prov‐ince .Methods Peripheral blood samples were collected from 17 522 preparing pregnant couples of child bearing age .Mean corpus‐cular volume (MCV) <80 fL was used as the positive indication for screening thalassemia .And 348 positive samples were detected by molecular biological technique for further analysis .Statistical analysis was conducted based on the couple′s genetic test results . Results Among the people at reproductive age ,the detection rates of couples bearing babies with Hb Bart′s Hydrops Fetalis ,He‐moglobin H disease andβ‐thalassemia were 0 .36% ,0 .47% and 0 .20% respectively ,the total was 1 .03% .The occurrence rates of birth defect caused by the above severe thalassemia were 9 .0/10 thousand ,11 .8/10 thousand and 5 .0/10 thousand ,the total was 25 .8/10 thousand .Conclusion This research illustrated the degree of damage of birth defect caused by severe thalassemia among the people at reproductive age ,which could provide valuable fundamental materials for the launch of eugenic work in this region .

Serum ?-carotene contents of both the healthy adults and the patients with malignant tumour diagnosed by pathology were measured in Shenyang city. It was found that the levels of ?-carotene in the tumour group were obviously lower than those of the healthy adults (p

To investigate the changes in serum enzymes (ALP、LDH、CK) in dogs suffered from gunshot wounds in limbs in hot and humid environment, 12 dogs were divided into two groups randomly: hot and humid environment (HHE) group and normal environment (NE) group. The contents of ALP, LDH and CK in peripheral blood were assayed at 0h, 2h, 4h, 6h, 8h, 12h and 24h postinjury. The results showed that the contents of serum enzymes tended to rise after gunshot injuries, especially CK. The changes in the contents of serum enzymes in HHE group appeared earlier and more obviously than those in NE group. In conclusion, the contents of serum enzymes coincided with the severity of local tissue damage, and change in the content of CK was more sensitive after tissue injury. The results suggested that hot and humid environment would aggravate systemic reactions and tissue damage after gunshot injuries.

To explore the prevention of deterioration of damaged tissue in gunshot wounds in limbs in hot and humid environment, 18 healthy male mongrel dogs were divided into three groups randomly: hot and humid environment (HHE) group, normal environment (NE) group, and heat acclimatization (HA) group. Histopathological changes and bacterial quantity in wound tracks were observed at postinjury o, 1, 4, 6, 8, 12 and 24h. It was found that the swelling of limbs and local pathological changes in injured tissues of HA group were significantly less serious and appeared later than those of HHE group, but severer and appeared earlier than those of NA group. The period that the bacterial quantity reaching the critical point of infection was 8h in HHE group, 12h in HA group and NE group. It is concluded that the pathological changes in gunshot injury in dogs inflicted in hot and humid environment could be lessened by exercise with heat acclimatization.

AIM:To investigate whether minocycline postconditioning protects rat myocardium from ischemia-reperfusion ( I/R ) injury through attenuating poly ( ADP-ribose ) polymerase-1 ( PARP-1 ) excessive activation. METHODS:The left anterior descending coronary artery was ligated for 45 min and then reopened for 2 h to establish the rat model of myocardial ischemia-reperfusion injury.The male Wistar rats ( n =90 ) were randomly divided into sham group, I/R group, low-and high-dose minocycline groups, and 3-aminobenzamide (3-AB, PARP inhibitor) group.The myocardial infarct size was measured by Evans blue and 2,3,5-triphenyltetrazolium chloride ( TTC) staining.The morpho-logical changes of the myocardium were observed with HE staining.The cardiomyocyte apoptosis was detected using in situ TDT-mediated dUTP nick end labeling ( TUNEL) .The level of tumor necrosis factorα( TNF-α) and interleukin 1β( IL-1β) in the serum were measured by ELISA.The content of poly( ADP-ribose) ( PAR) in the reperfused myocardium and peripheral leukocytes were detected by Western blot.RESULTS: Compared with sham group, PAR expression, TNF-αcontent and IL-1βconcentration increased in all other groups.Compared with I/R group, treatment with low and high doses of minocycline and 3-AB significantly reduced the infarct size and myocardial apoptosis.PAR expression, TNF-αcontent and IL-1βconcentration in low-and high-dose minocycline groups and 3-AB group all decreased.No significant difference of the above parameters between high-dose minocycline group and 3-AB group was observed.CONCLUSION: Minocy-cline postconditioning may attenuate myocardial ischemia-reperfusion injury by depressing the activation of PARP-1 in car-diomyocytes and peripheral leukocytes in rats.

Objective To discuss a new method of BMSCs culture which can reduce the lead-in of extraneous antigenic substances in the process of cell culture, and can thus better meet the demand on the quantity and biological characteristics of BMSCs in the clinical use of tissue engineering bone. Methods BMSCs from a human adult in vitro were induced by using autologous platelet-rich plasma instead of animal serum in high-carbohydrate DMEM (Dulbecco's minimum essential medium). After the cultured BMSCs were amplified rapidly by Rotary Cell Culture System, their growth status was observed and their biological characteristics were detected. Results The cells were in good growth status and presented fine biological characteristics. Their growth speed in Rotary Cell Culture System was observed to be evidently faster than that by conventional methods. Conclusions Rotary Cell Culture System by autologous platelet-rich plasma instead of animal serum is a good culture method to induce human BMSCs in vitro. The quantity and biological characteristics of BMSCs cultured in this way can meet the demand in clinical use.

Objective To discuss the clinical effect about flyback avulsion injury of skin in foot that repaired by anterolateral thigh flap and sural neurovascular flap with their neural anastomosis.Methods 14 patients including 10 males and 4 females,from 16 to 58 years old,with an average of 31 years.The anterolateral thigh flap taking along anterolateral thigh cutaneous nerve was cut to repair wall and sole of foot,at the same time,the anterolateral thigh cutaneous nerve and the medial plantar nerve was anastomosis;the lateral plantar nerve was embedded in the anterolateral thigh flap that was used to repair sole of foot.The sural neurovascular flap was reversed to repair lateral part of foot,and the superficial peroneal nerve was sutured with sural exterior and interior cutaneous nerve.According to Swanson evaluation of clinical effect of peripheral nervous impairment,the sensation of flap was classed as S1,S2,S3,S4,S5;the area of sensory recovery was classed as R1(

Objective To investigate the angiographic coronary atheroslerosis findings with the plasma levels of von Willebrand factor (vWF) and ?-granule membrane protein (GMP-140). Methods 74 patients undergone selectrive coronary angiography (CAG) were divided into 3 groups based on plaque morphology, Group S(n=15), concentric or eccentric stenosis with smooth borders; Group C(n=37), eccentric stenosis with complex borders; Group N (n=22), CAG without coronary atheroslerosis. 37 patients in group C were divided into group Ⅰ (n=10, one-vessel involved CAD), group Ⅱ (n=12, two-vessel involved CAD) and group Ⅲ (n=15, three-vessel involved CAD) based on major epicardial coronary branches lesion. These 37 patients were divided into group x(n=21, ≤3 segments) and group y(n=16,≥4 segments) based on coronry stenotic segments. The plasma levels of vWF and GMP-140 were assayed by ELISA before angiography. Results (1)The plasma levels of vWF and GMP-140 in group C were significantly higher than those in group S(P

BACKGROUND:Double inferior vena cava (DIVC), usually found at routine autopsies, is rare in the clinic. It has been described in numerous reports over the years since Adachi first depicted it in 1940. But all these reports were based on two-dimensional (2D) observation, and no three-dimensional and animated images reported. OBJECTIVE: To observe and establish the digital visible models of the structure of DIVC of the Virtual Chinese Human Male (VCH-M) Ⅲ.DESIGN: Repetitive measurement design by taking VCH-M Ⅲ as the subjects.SETTING : Department of Traumatic Orthopedics and Anatomy, Nanfang Hospital Affiliated to Southern Medical University.MATERIALS: The experiment was conducted in Southern Medical University from February 2006 to May 2006. The cross-sectional images of fresh tissues from VCH-M Ⅲ dataset were reviewed and 1 060 serially-sectioned slices of VCH-M Ⅲ, from the 2 600th slice to the 3 660th were taken as the source for the 3D models in our study.METHODS: Cross-sectional images of fresh tissues from the VCH-M Ⅲ dataset were reviewed and the structures of the DIVC were confirmed on a section-by-section basis. 3D computerized reconstructions of DIVC and its adjacent structures were generated from these data using Arnira 3.1 (TGS) imaging software.MAIN OUTCOME MEASURES: Cross-sectional images and 3D reconstruction of VCH-M Ⅲ.RESULTS: The cross-sectional images of the VGH-M Ⅲ could fairly display the main structure of DIVC. The digitized model of brachial plexus offers unique insights into the abnormity anatomy of DIVC.CONCLUSION: The VCH-M Ⅲ dataset can provide complete and accurate data of DIVC.

To explore the effect of heat acclimatization to the content of SOD and MDA in dogs with gunshot wound in limbs in hot and humid environment, eighteen Chinese mongrels were divided into 3 groups randomly according to the given environment: heat acclimatization(HA) group, normal environment(NE)group, and hot and humid environment(HHE) group. The contents of superoxide dismutast(SOD) and malonaldehyde(MDA) in muscle tissues of the gunshot wound track were assayed at 0, 1, 3, 4, 6, 8, 10, 14, 18 an 24h respectively after wounding. The results showed that the contents of MDA in both HA and HHE groups began to ascend on postinjury hour(PIH) 1, peaking at PIH10 in HA group and PIH6 in HHE group, with the former lower than the latter. There was no significant difference between the contents of MDA in the three groups at PIH18. The content of SOD in HA group began to rise immediately after injury, which was 1h earlier than that in the other two groups, and it was higher than that in HHE group after PIH4. The content of SOD was too low to be measured at PIH10 in HHE group, and this result was 4h earlier than that in the other two groups. In conclusion, SOD and MDA play an important role in the pathophysiological process of gunshot wound in hot and humid environment. Heat acclimatization can be favorable to mitigate pathophysiological changes, and it might be an effective method to prevent rapid deterioration of gunshot injuries in hot and humid environment.