Serum ?-carotene contents of both the healthy adults and the patients with malignant tumour diagnosed by pathology were measured in Shenyang city. It was found that the levels of ?-carotene in the tumour group were obviously lower than those of the healthy adults (p

Objective To investigate the detection rate of couples at high risk of bearing babies with severe thalassemia and the occurrence rate of birth defect caused by severe thalassemia among the people at reproductive age in Foshan city ,Guangdong prov‐ince .Methods Peripheral blood samples were collected from 17 522 preparing pregnant couples of child bearing age .Mean corpus‐cular volume (MCV) <80 fL was used as the positive indication for screening thalassemia .And 348 positive samples were detected by molecular biological technique for further analysis .Statistical analysis was conducted based on the couple′s genetic test results . Results Among the people at reproductive age ,the detection rates of couples bearing babies with Hb Bart′s Hydrops Fetalis ,He‐moglobin H disease andβ‐thalassemia were 0 .36% ,0 .47% and 0 .20% respectively ,the total was 1 .03% .The occurrence rates of birth defect caused by the above severe thalassemia were 9 .0/10 thousand ,11 .8/10 thousand and 5 .0/10 thousand ,the total was 25 .8/10 thousand .Conclusion This research illustrated the degree of damage of birth defect caused by severe thalassemia among the people at reproductive age ,which could provide valuable fundamental materials for the launch of eugenic work in this region .

To investigate the changes in serum enzymes (ALP、LDH、CK) in dogs suffered from gunshot wounds in limbs in hot and humid environment, 12 dogs were divided into two groups randomly: hot and humid environment (HHE) group and normal environment (NE) group. The contents of ALP, LDH and CK in peripheral blood were assayed at 0h, 2h, 4h, 6h, 8h, 12h and 24h postinjury. The results showed that the contents of serum enzymes tended to rise after gunshot injuries, especially CK. The changes in the contents of serum enzymes in HHE group appeared earlier and more obviously than those in NE group. In conclusion, the contents of serum enzymes coincided with the severity of local tissue damage, and change in the content of CK was more sensitive after tissue injury. The results suggested that hot and humid environment would aggravate systemic reactions and tissue damage after gunshot injuries.

To explore the prevention of deterioration of damaged tissue in gunshot wounds in limbs in hot and humid environment, 18 healthy male mongrel dogs were divided into three groups randomly: hot and humid environment (HHE) group, normal environment (NE) group, and heat acclimatization (HA) group. Histopathological changes and bacterial quantity in wound tracks were observed at postinjury o, 1, 4, 6, 8, 12 and 24h. It was found that the swelling of limbs and local pathological changes in injured tissues of HA group were significantly less serious and appeared later than those of HHE group, but severer and appeared earlier than those of NA group. The period that the bacterial quantity reaching the critical point of infection was 8h in HHE group, 12h in HA group and NE group. It is concluded that the pathological changes in gunshot injury in dogs inflicted in hot and humid environment could be lessened by exercise with heat acclimatization.

Objective To discuss the three-dimensional reconstruction of lateral flaps of legs by Amira 4.1 based on an operational platform of personal computer,and to establish their digitized visible models.Methods A fresh adult cadaver specimen was perfused with the mixture of lead oxide-gelatine.The eikonic data were imputed into personal computer.Radiographs of CT scanning of the lower legs were digitally analyzed by Amira 4.1 software.The segmented areas for skin,arteries and flap structures were displayed in different colors using tools of Brush,Lasso and Magic wand.By several steps,according to particularities of anatomy structures and traits of images,stereo images of the flap and its blood vessel structure were performed by several algorithms.The 3D reconstruction via the Amira 4.1 software consisted of tracing the contours of the anatomical structures to be reconstructed;adjusting the contours of stacked points by geometrical alignment;modeling the surfaces by meshing the framework of the points transformed into polygons and smoothing the contours of the object reconstructed from points.Three dimensional computerized reconstructions of lateral flaps of legs were conducted from these data using Amira 4.1 by computer-assisted imaging processing.Results The 3D images of the flap and its blood vessel structure by Amira were clearly displayed,and the surface and volume information could be obtained.The 3D images could display perfectly the main structures of flap and other adjacent structures by means of personal computer and software Amira 4.1.Correlation of cross-sectional images from CT-scanning to 3D models was a more effective way for understanding the flap anatomy.Conclusion Three-dimensional computerized reconstruction for lateral flaps of legs may provide great value for clinical experiments,basic research and surgical planning,and the Amira 4.11 software is of vital for three-dimensional reconstruction.

Objective: To evaluate the clinical effectiveness and safety of early total enteral nutrition (TEN) in severely burned patients. Methods: Forty one burned patients with total burn surface area over 30% were randomly assigned to TEN group (n=21) and CONT group (n=20). The two nutritional support protocols were similar in calorie and nitrogen intake within 7 d of treatment. Serum levels of visceral proteins and TNF-? were measured. Prognostic inflammatory and nutritional index (PINI) was postulated from formula. Results: Contrasting to a significant reduction (from 96.0?31.8mg/L to 69.4?17.3 mg/L, P

Objective To discuss the clinical effect about flyback avulsion injury of skin in foot that repaired by anterolateral thigh flap and sural neurovascular flap with their neural anastomosis.Methods 14 patients including 10 males and 4 females,from 16 to 58 years old,with an average of 31 years.The anterolateral thigh flap taking along anterolateral thigh cutaneous nerve was cut to repair wall and sole of foot,at the same time,the anterolateral thigh cutaneous nerve and the medial plantar nerve was anastomosis;the lateral plantar nerve was embedded in the anterolateral thigh flap that was used to repair sole of foot.The sural neurovascular flap was reversed to repair lateral part of foot,and the superficial peroneal nerve was sutured with sural exterior and interior cutaneous nerve.According to Swanson evaluation of clinical effect of peripheral nervous impairment,the sensation of flap was classed as S1,S2,S3,S4,S5;the area of sensory recovery was classed as R1(

To investigate the dynamic change of TNF ? and HSP70 after gunshot wound on limbs of canine in hot and humid environment, the canines were divided into three groups: trauma in normal environment group(NE), trauma in heat acclimatization group (HA), trauma in hot and humid environment group(HHE). Tumor necrosis factor ? (TNF ?) and HSP70 were measured at the time of 0, 1, 3, 4, 6 ,8 , 10, 14, 18, 24h after canine being gunshot. The results showed: ①TNF ? in muscle tissue from gunshot wound tract was significantly higher than that in blood plasma( P

Objective To discuss a new method of BMSCs culture which can reduce the lead-in of extraneous antigenic substances in the process of cell culture, and can thus better meet the demand on the quantity and biological characteristics of BMSCs in the clinical use of tissue engineering bone. Methods BMSCs from a human adult in vitro were induced by using autologous platelet-rich plasma instead of animal serum in high-carbohydrate DMEM (Dulbecco's minimum essential medium). After the cultured BMSCs were amplified rapidly by Rotary Cell Culture System, their growth status was observed and their biological characteristics were detected. Results The cells were in good growth status and presented fine biological characteristics. Their growth speed in Rotary Cell Culture System was observed to be evidently faster than that by conventional methods. Conclusions Rotary Cell Culture System by autologous platelet-rich plasma instead of animal serum is a good culture method to induce human BMSCs in vitro. The quantity and biological characteristics of BMSCs cultured in this way can meet the demand in clinical use.

BACKGROUND:Double inferior vena cava (DIVC), usually found at routine autopsies, is rare in the clinic. It has been described in numerous reports over the years since Adachi first depicted it in 1940. But all these reports were based on two-dimensional (2D) observation, and no three-dimensional and animated images reported. OBJECTIVE: To observe and establish the digital visible models of the structure of DIVC of the Virtual Chinese Human Male (VCH-M) Ⅲ.DESIGN: Repetitive measurement design by taking VCH-M Ⅲ as the subjects.SETTING : Department of Traumatic Orthopedics and Anatomy, Nanfang Hospital Affiliated to Southern Medical University.MATERIALS: The experiment was conducted in Southern Medical University from February 2006 to May 2006. The cross-sectional images of fresh tissues from VCH-M Ⅲ dataset were reviewed and 1 060 serially-sectioned slices of VCH-M Ⅲ, from the 2 600th slice to the 3 660th were taken as the source for the 3D models in our study.METHODS: Cross-sectional images of fresh tissues from the VCH-M Ⅲ dataset were reviewed and the structures of the DIVC were confirmed on a section-by-section basis. 3D computerized reconstructions of DIVC and its adjacent structures were generated from these data using Arnira 3.1 (TGS) imaging software.MAIN OUTCOME MEASURES: Cross-sectional images and 3D reconstruction of VCH-M Ⅲ.RESULTS: The cross-sectional images of the VGH-M Ⅲ could fairly display the main structure of DIVC. The digitized model of brachial plexus offers unique insights into the abnormity anatomy of DIVC.CONCLUSION: The VCH-M Ⅲ dataset can provide complete and accurate data of DIVC.