Objective To observe the effect of transformation of vascularized tissue-engineered bone with vascular bundle graft in vivo. Methods The bone marrow stem cells (BMSCs) obtained from rabbit os ilium were cultured, expanded and induced to form osteocytes, then they were combined with porous ?-tricalcium phosphate (?-TCP) to construct tissue-engineered bone. The tissue-engineered bone was implanted in a segmental defect with critical length of 15mm in rabbit femoral shaft. A bundle containing both artery and vein was inserted in the centre of the tissue-engineered bone with microvascular surgical technique. After an examination with DEXA bone scanner, the specimen was harvested for macroscopic and histological examination after 12 weeks of growth period. Results The site where the implanted vascular bundle inserted into the tissue engineered bone appeared like foramen nutriens, the blood vessels were patent. Abundant blood vessels, which emerged from the deep tissue of the engineered bone, were evident on the surface of specimen. Multicentric ossification area rich in blood vessels could be seen in the tissue-engineered bone histologically. The enchondral ossification in the center of tissue-engineered bone and membranous ossification in the periphery occurred simultaneously. Some materials in the centre of tissue-engineered bone had transformed into marrow cavity like construction. The results of the DEXA demonstrated that the vascularized tissue-engineered bone produced more boney tissue. Conclusions Implantation of blood vessel bundle into tissue-engineered bone may enhance neovascularization of the tissue-engineered bone and accelerate the process of reconstruction subsequently, and it is a promising method of vascularization of tissue-engineered bone.

Field medical equipment is important material base of medical service in wartime and one organic part of medical care scheme in echelon. However, medical service under hot and humid environment (HHE) is characterized by some new features due to the dual influences of environmental factor and war injury itself, which might bring certain challengeable demands on medical equipment development. As one part of medical care scheme in echelon under HHE, this paper presents a general study on demands of field medical equipment under HHE aimed to provide references for demonstration, research and development of medical equipment of Chinese PLA in the future.

Vascularization is one of the major c hallenges in tissue engineering gra ft.Vascular plexus falls into two modes which are different in recourse of endotheliocyte:vascul ogenesis and angiogenesis.Both mod es are a dynamic and complicated physiological process.Various kinds of growth factor and adhesion molecule modula te the process.Both in vitro and in vivo methods are used in vascularization research.In vitro methods are used to e-valuate biomaterials for endothelial cell attachment,cytotoxicity,g rowth,angiogenesis and its effects on gene reg-ulation.Chorioallantoic membrane(CAM)is one of the simplest and effective models in vivo.Animals are used as more advanced models.The strategie s of vascularization of tissue engin eering include:modification of the surface of the biomaterials,addition of slow-release growth factor to scaffold,e ndotheliocyte cocultured with other seed cells,wrapping by vascular net,implantin g blood vessel bundles into the graft,seeding biomaterial implants with vascular remnants as vascular template,and c reating perfused microvessels in vitro.None of these approaches,however,is satisfactory at present.We believe that an ideal method will be finally f ound for vascularization in tissue e ngineering with advancement of research on mech anism of vascularization and progre ss in techniques.[

Objective To study the clinical value of serum cardiac troponin Ⅰ (cTnⅠ) in patients with unstable angina pectoris (UAP) and minor myocardial damage.Methods The serum cTnⅠ level of 60 UAP patients (UAP group) were determined.The patients whose cTnⅠ were ≥ 0.1 μ g/L were ascribed to cTnⅠ positive group,and the patients whose cTnⅠ were ＜ 0.1 μ g/L were ascribed to cTnⅠ negative group.The correlation among clinical data,coronary arterial lesions and adverse cardiac events were analyzed.Forty healthy people were selected as control group.Results The positive rate of cTnⅠ in UAP group (35.0％,21/60) was significantly higher than that in control group (0),and there was statistical difference (P ＜ 0.01).The incidence rate of coronary arterial multi-branch lesions and/or severe stenosis in cTnⅠ positive group (19.0％,4/21) was higher than that in cTnⅠ negative group (5.1％,2/39),but there was no statistical difference (P ＞ 0.05).The incidence rate of 90 d adverse cardiac events in cTnⅠ positive group (33.3％,7/21) was significantly higher than that in cTnⅠ negative group (5.1％,2/39),and there was statistical difference (P ＜ 0.01).Conclusions Low-level cTnⅠ demonstrates minor myocardial damage sensitively and specifically.cTnⅠ level detected quantitatively can help to assess prognosis and provide risk stratification of UAP patients.

Objective To study the osteogenic capability of bone marrow stromal cell (BMSc) using tissue engineering methods Methods The osteogenic potential in vitro of cultured BMSc in a conditional medium were examined by phase contrast microscopy,histochemistry stains technique The BMSc were cultured in combination with bioactive glass ceramic (BGC) materials Then the composite were implanted into the skeletal muscle beds in rabbits All implants were exmined by gross observation and histological examination Results The BMSc showed a similar property to those of osteoblasts and could synthesized mineralized new bone tissue in vitro New bone tissue can be observed in the composites of the BMSc and BGC materials Conclusions New bone tissue can be formed by tissue engineering methods

Objective To investigate the angiographic coronary atheroslerosis findings with the plasma levels of von Willebrand factor (vWF) and ?-granule membrane protein (GMP-140). Methods 74 patients undergone selectrive coronary angiography (CAG) were divided into 3 groups based on plaque morphology, Group S(n=15), concentric or eccentric stenosis with smooth borders; Group C(n=37), eccentric stenosis with complex borders; Group N (n=22), CAG without coronary atheroslerosis. 37 patients in group C were divided into group Ⅰ (n=10, one-vessel involved CAD), group Ⅱ (n=12, two-vessel involved CAD) and group Ⅲ (n=15, three-vessel involved CAD) based on major epicardial coronary branches lesion. These 37 patients were divided into group x(n=21, ≤3 segments) and group y(n=16,≥4 segments) based on coronry stenotic segments. The plasma levels of vWF and GMP-140 were assayed by ELISA before angiography. Results (1)The plasma levels of vWF and GMP-140 in group C were significantly higher than those in group S(P

To prepare an experimental caprine model of tibia bone diaphyseal defect used in tissue engineering and defect repair with the tissue engineering method, 27 Chinese caprines were divided into 3 groups: blank, control, and test. The 20mm left tibia diaphyseal defect of each caprine was made and fixed with plates. The blank group was not filled with coral hydroxyapatite (CHAP) or bone marrow stroma cell (BMSc). The control group and the experimental group were filled with CHAP and CHAP/BMSc respectively. The results of three groups were evaluated by X ray examination, optical density index of X ray film and histology 4, 8, 12 weeks after operation. The biomechanical characteristics of the specimens of the CHAP group and the CHAP/BMSc group were tested by three point bending test 12 weeks after operation.The results showed that the optical density indices of X ray film of the blank group were not significantly different 4, 8, 12 weeks after operation and indices of the CHAP group were significantly smaller than those of the CHAP/BMSc group ( P

Objective　To study the biocompatibility of bioactive glass ceramic (BGC) materials with bone marrow stromal cell (BMSc) and the osteogenic capability of BMSc using tissue-engineering methods. 　Methods　The osteogenic potential in vitro of cultured BMSc in a conditional medium was examined by histochemistry stains technique. The BMSc was cultured in combination with BGC. The attaching and extending speed of the cells to the materials, the proliferation and alkaline phosphatase activity were tested. Then the composite was implanted into the skeletal muscle beds in rabbits. All implants were examined by gross observation and histological examination. 　Results 　The BMSc showed a similar property to those of osteoblasts. BMSc can attach to and extend on BGC materials. No inhibition to celluar proliferation and ALP activity were observed by the materials. New bone can be observed in the composites of the BMSc and BGC materials.　 Conclusions　 BMSc may provide a rich cellular resource in tissue-engineered bone formation. New bone tissue can be formed by tissue engineering methods.

Objective To discuss the diagnosis and operative treatment of the complex acetabular fractures of Letournel classification. Methods On the basis of the three- dimensional computed tomography, 75 cases of complex acetabular fractures were diagnosed and classified according to Letournel classification. They were treated through the anterior, posterior, combined anterior- posterior and the improved iliofemoral approaches. All the fractures were fixed with screws and AO reconstruction plates. Results All the cases were followed up for 6 to 96 months, with an average time of 46 months. They were evaluated according to D' Aubigne and Pestel criteria for joint functions and Epstein criteria for radiographic manifestation. 34 cases of the series were rated as excellent (45.23% ), 28 case as fine (37.33% ), 8 cases as fair (10.67% ) and 5 cases as poor (6.67% ). Conclusion Enough image data, simulation in vitro on a pelvic specimen, maximal anatomical reduction and appropriate approach are the basis for satisfactory outcomes.

Objective To investigate the effect and mechanism of different high concentrations glucose(Glu) on the expression of hypoxia-inducible factor 1α(HIF-1α) in cultured neonatal rat eardiomyocytes under hypoxia and non-hypoxia conditions. Methods Neonatal rat cardiomyocytes were cultured for 6 hours under different conditions and were divided into 6 groups:①Negative control group (5.5 mmol/L Glu) ; ②hypoxia mimicking cobalt chloride(Cocl_2) group(5.5 mmol/L Glu + 400 μmol/L cocl_2 ) ;③Different high concentrations Glu groups: (11.1 mmol/L, 22.2 mmol/L, 33.3 mmol/L Glu);④cocl_2 +different high concentrations Glu groups(11.1 mmol/L Glu +400 μmol/L cocl_2,22.2 mmol/L Glu +400 μmol/L cocl_2, 33.3 mmol/L Glu+400 μmol/L cocl_2);⑤High concentrations Glu + antioxidant α-tocopherol group(33.3 mmol/L Glu + 100 μmol/L α-tocopherol) ; ⑥ High concentrations Glu+antioxidant α-tocopherol+cocl_2 group(33.3 mmol/L Glu+400 μmol/L cocl_2+100 μmol/L α-tocopherol). The effect of high concentrations Glu, cocl_2 and high concentrations Glu plus cocl_2 on the expression of HIF- 1α mRNA and protein in cultured neonatal rat cardiomyocytes, as well as the effect of high concentrations Glu plus antioxidant α-tocopherol, high Glu concentrations plus cocl_2 and antioxidant α-tocopherol on the expression of HIF- 1α mRNA and protein were observed. Results 1. Compared with negative control group(5.5 mmol/L Glu), the 'expression of HIF- 1α was increased under cocl_2 mimicked hypoxia(5.5 mmol/L Glu+400 μmol/L cocl_2, 11.1 mmol/L Glu+400 μmol/L cocl_2 ,22.2 mmol/L Glu+400 μmol/L cocl_2,33.3 mmol/L Glu+400 μmol/L cocl_2). 2. The expression of HIF- 1α was increased gradually after the increasing of Glu concentrations(5.5 mmol/L, 11.1 mmol/L, 22.2 mmol/L and 33.3 mmol/L Glu).3.The expression of HIF-1α was decreased gradually after the increasing of Glu concentrations under certain cocl_2 plus different high concentrations Glu (5.5 mmol/L Glu+400 μmol/L cocl_2, 11.1 mmol/L Glu+400 μmol/L cocl_2, 22.2 mmol/L Glu+400 μmol/L cocl_2,33.3 mmol/L Glu+400 μmol/L cocl_2). 4. Under high concentration Glu plus antioxidant α-tocopherol (33.3 mmol/L Glu+100 μmol/L α-tocopherol) ,the expression of HIF- 1α was less increased than the same high Glu concentration(33.3 mmol/L Glu). 5. Under high Glu concentration plus cocl_2 (33.3 mmol/L Glu+400 μmol/L cocl_2), the expression of HIF- 1α increased less than that of the same high Glu concentration plus antioxidant α-tocopherol and cocl_2 (33.3 mmol/L Glu+400 μmol/L Cocl_2+ 100 μmol/L α-tocopherol). Conclusions High glucose increases the expression of HIF-1α under non-hypoxia, but blunts the expression of HIF-1α under hypoxia in cultured neonatal rat cardiomyocytes. Some mechanisms such as ROS (reactive oxygen species) signal transduction system and oxidative stress may be involved in it.