Objective: To develop a novel fast auto blood viscosity analyzer based on the relation between flow rate and Casson viscosity of non-Newton fluid such as blood. Methods: Different from the tradition friction method of measuring blood viscosi-ty, the instrument was developed based on the Casson flow equation for blood in round tube and Stokes equation, using virtual instrument technique to calculate the Casson viscosity and stress, and pressure sensor and peristaltic pump to perform a setup. Results: Controlled by the data acquisition system, the blood Casson viscosity and stress were measured quickly and simply. Conclusions: Compared with the similar instruments used in clinic, this analyzer is faster in measurement and has high preci-sion, can determine the apparent viscosity and other Casson parameters of blood at any shear rate, and gives more clinic informa-tion for patients.

Objective To investigate the detection rate of couples at high risk of bearing babies with severe thalassemia and the occurrence rate of birth defect caused by severe thalassemia among the people at reproductive age in Foshan city ,Guangdong prov‐ince .Methods Peripheral blood samples were collected from 17 522 preparing pregnant couples of child bearing age .Mean corpus‐cular volume (MCV) <80 fL was used as the positive indication for screening thalassemia .And 348 positive samples were detected by molecular biological technique for further analysis .Statistical analysis was conducted based on the couple′s genetic test results . Results Among the people at reproductive age ,the detection rates of couples bearing babies with Hb Bart′s Hydrops Fetalis ,He‐moglobin H disease andβ‐thalassemia were 0 .36% ,0 .47% and 0 .20% respectively ,the total was 1 .03% .The occurrence rates of birth defect caused by the above severe thalassemia were 9 .0/10 thousand ,11 .8/10 thousand and 5 .0/10 thousand ,the total was 25 .8/10 thousand .Conclusion This research illustrated the degree of damage of birth defect caused by severe thalassemia among the people at reproductive age ,which could provide valuable fundamental materials for the launch of eugenic work in this region .

The clinical data of 23 cases with emergent hematochezia endoscopically diagnosed as non-ulcerous duodenum diseases were retrospectively analyzed.Majority of cases were misdiagnosed and delayed to transfer to emergency room because of lack of knowledge and experiences of local medical workers.After referral to our emergency department,all 23 cases obtained appropriate management and cured successfully.

Objective To establish a 3D atlas of Letournel-Judet classifications of acetabular fractures which provides remote access on the web through hypertext markup language (HTML) and virtual reality modeling language (VRML) for efficient remote diagnosis. Methods Firstly,different Letournel-Judet classifications of acetabular fracture were simulated based on 3D reconstruction models of healthy volunteers via Mimics software.The files of VRML format were exported after geometry modeling.Secondly,The.wrl files were edited by adding nodes of Sensor,Route,and Script to the files to make the static scene respond to users' interaction.Finally,the homepage of 3D atlas was established with the Dreamweaver software.Results The 3D atlas of Letournel-Judet classifications of acetabular fractures was established which can be accessed online.These virtual worlds can be browsed via the Internet and hyperlinked with the World Wide Web. Conclusion The 3D atlas of Letournel-Judet classifications of acetabular fractures can be easily and convenicntly used by surgeons for consultation in clinical diagnosis of an acetabular fracture.

Objective To discuss the clinical effect about flyback avulsion injury of skin in foot that repaired by anterolateral thigh flap and sural neurovascular flap with their neural anastomosis.Methods 14 patients including 10 males and 4 females,from 16 to 58 years old,with an average of 31 years.The anterolateral thigh flap taking along anterolateral thigh cutaneous nerve was cut to repair wall and sole of foot,at the same time,the anterolateral thigh cutaneous nerve and the medial plantar nerve was anastomosis;the lateral plantar nerve was embedded in the anterolateral thigh flap that was used to repair sole of foot.The sural neurovascular flap was reversed to repair lateral part of foot,and the superficial peroneal nerve was sutured with sural exterior and interior cutaneous nerve.According to Swanson evaluation of clinical effect of peripheral nervous impairment,the sensation of flap was classed as S1,S2,S3,S4,S5;the area of sensory recovery was classed as R1(

[Objective]To study the ossification effect by histology research on tissue engineered bone by transplanting nerval bundles of different types.[Method]The Newzeland rabbit BMSCs(bone marrow stromal cells,BMSCs) were induced to osteoblast and compounded with ?-TCP(?-tricalcium phosphate,?-TCP)to make tissue engineered bones,and different kinds of nerval bundles were transplanted into these tissue engineered bone.All Newzeland rabbits were divided into 5 groups according to different types of nerval bundles:Group A,tissue engineered bones without nerval bundles transplantation.Group B,tissue engineered bones transplanted with sensory nerval bundles.Group C,tissue engineered bones transplantation with motor nerval bundles.Group D,tissue engineered bones transplanted with vascular bundles(autonomic nerves contained).Group E,tissue engineered bones transplantation with both sensory and motor nerval bundles.Then,1.5 cm bone defect in femur of Newzeland rabbits were repaired.At 12 week,gross specimen observation,histology studies and immunohistochemistry were used to compare the results of bone repair in different groups.[Result]During the observation period,tissue engineered bones transplanted with sensory and vascular bundles effectively accelerated the ossification of the tissue engineered bones,while those transplanted with motor nerval bundles did not.[Conclusion]Sensory nerval bundles and vascular bundles can effectively accelerate the ossification of tissue engineered bones.They can be used as a construction method for nervalization after tissue engineered bone.

Objective To investigate the effects of local delivery c-myc antisense oligodexynucleotides on neointimal hyperplasia after angioplasty in rabbits. Methods The celiac arterial endothelium of 75 male Japanese white rabbit (2.5?0.5 kg) were denuded after being fed with cholesterol-supplemented diet to establish the model of atherosclerotic stenosis of celiac arteries. The animals were randomized into the antisense DNA group (Group Ⅰ), sense DNA group (Group Ⅱ), saline control group (Group Ⅲ) and simple angioplasty group (Group Ⅳ). The drugs were injected intramurally using a porous balloon. The local vessels were collected at the 24 th hour and the 4 th week after the procedure for morphological observation. C-myc mRNA level was measured by means of RT-PCR and c-myc protein with immunohistochemical staining. Results RT-PCR showed that c-myc mRNA level in Group Ⅰ was lower than that of Group Ⅱ, Group Ⅲ and Group Ⅳ after the first 24 hours. The ratio of neointimal area (NEA) to medial area (MA) and NEA/MA in Group Ⅰ were significantly less than those of Group Ⅱ, Group Ⅲ and Group Ⅳ. Immunohistochemical staining showed that the percentage of c-myc protein positive cells was less in Group Ⅰ than those of the other groups. Conclusion Transcatheter local delivery of c-myc antisense oligonucleotides reduces neointimal hyperplasia after angioplasty in rabbits.

Objective To study the relative factors of hypotension after recanalization of infarction related artery(IRA) during emergency PCI for patients with acute myocardial infarction.Methods We retrospectively analyzed the clinical information and the coronary angiograph of 245 consecutive patients with primary acute myocardial infarction undergoing emergency PCI at the first affiliated hospital of China Medical University from January 2003 to September 2006.Twenty seven patients with blood pressure elevated higher than 140/90 mm Hg after reperfusion were excluded.The remaining 218 patients were catagorized into the hypotension group(BP≤90/60 mm Hg,n=102) and the normal blood pressure group(n=116) according to the blood pressure level after reperfusion during emergency PCI.The relative factors of hypotension were analyzed through logistic regression test.Results Compared with the normal blood pressure group,patients in the hypotension group had higher incidences of old age(age ≥60 yrs),heart failure(killip grades≥grade 2),inferior wall or right ventricular infarction,right coronary artery lesions,proximal or total occlusion lesions,multivessal disease and TIMI blood flow≤grade 1 in the IRA after recandization.Mortality rate was also higher in the hypotension group(P1).Conclusion Old age(age ≥60),inferior wall infarction,killip grades≥2,right coronary artery lesions,proximal or total occlusion lesions are the risk factors of hypotension after recanalization of IRA during emergency PCI.The presence of hypotension is correlated with the increase of in hospital mortality.

The therapeutic effect of a natural foodstuffs--Wei-Luo powder (WLP)composed of high protein, various essential amino acids, vitamins (B1, B2 and E), and trace elements (Fe, Zn, etc), on both monosodium glutamate (MSG) induced obese mice and alloxan diabetic mice were tested. After 16 days of feeding with WLP diet, the body weight and accumulation of fat in liver and adipose tissue (Lee index) in MSG mice were decreased significantly, WLP diet also caused significant reduction in carbohydrate tolerance of mice as compared with wheat diet. In addition, improvement of hyperglycemia and diabetic symptoms (polydipsia, polyuria and hyperphagia) in alloxan diabetic mice were observed. These results suggest that WLP diet may be beneficial for obesity and diabetic patients.

BACKGROUND: Vascular endothelial growth factor (VEGF) can promote angiogenesis, and has been extensively used in treatment of bone defect. However, few studies have addressed its isomer VEGF121. OBJECTIVE: To construct adenovirus vector carrying VEGF121-FLAG and humanized Renilla reniformis green fluorescent protein 1(hrGFP-1) and observe its expression in bone marrow stromal stem cells (BMSCs). METHODS: Using polymerase chain reaction technique, VEGF121 gene contained in the plasmid of pTG19T-VEGF121 was used to remove termination codon. NotI and Xho I restriction sites were added before and after gene sequence. Obtained gene subclone was moved onto pMD19-T plasmid. The pMD19-T-VEGF121 and pShuttle-CMV-IRES-hrGFP-1 plasmids underwent double enzymatic digestion. Small fragment and big fragment were retrieved utilizing gel. Subsequently, coupled reaction was conducted to complete the construction of shuttle plasmid. After measuring virus titer, BMSCs were transfected and the fluorescence intensity was observed under fluorescence microscope.RESULTS AND CONCLUSION: Recombinant adenovirus plasmid was successfully constructed by enzymatic digestion determination and gene sequence. Fluorescence microscope has shown that BMSCs transfected with recombinant adenovirus presented significantly green fluorescence expression. Thus, adenovirus vector carrying VEGF121-FLAG and hrGFP-1 gene can express in eukaryotic cells, which can be used for gene therapy for ischemic disease.