Purpose:To explore the clinical significance of gene diagnosis in detecting micrometastases in lymph node,peripheral blood and bone marrow from non-small-cell lung cancer patients and the correlation to e ach other. Methods:The MUC1 mRNA expression was detected in lymph nodes, p eripheral blood and bone marrow from 31 lung cancer putients and 10 benign p?lm onary lesion patients by nested RT-PCR. Results:The sensitivity of the the RT-PCR technique was 10 -6 in this study. The positive rates of MUC1 mRNA were 54.6%, 32.3% and 22.6% in lymph nodes , peripheral blood and bone marrow from lung cancer patients resp ectively , and highly correlation existed among the three groups(P

Objective:By measuring the expression of nuclear factor of ?B(NF-?B) and apoptosis in the tissue of benign and malignant pancreatic diseases.Methods:The expression of NF-?B factor was measured by immunohistochemical method to measure.The expression of apoptosis for cells was determined by TUNEL method.Results: When measuring the expression of NF-?B p65 protein in the tissue,we found firstly,in the tissue of pancreatic cancer,NF-?B p65 protein was located in the nucleolus and cytoplasm,the number of positive cell was obviously more than that in the control group(P

Accordingtothe analysis ofthe characters and difficulties ofskill ofanimal surgery,we divide the teaching process into three stages:theory classes,aseptic technique and basic operating techniques,animal practice.PBL+LBL double-track teaching methods is performed,which can make up the defect of PBL in practice and should be advocated and spread in the teaching of skill of animal surgery.

Purpose　To detect the prevention of EGF and GLN on the side effect of TPN.　Methods　We randomized SD rats into four groups:group A (control),B(STPN),C(TPN+GLN),D(TPN+GLN+EGF).All rats were maintained on their respective diets for 8 days,at which time they were killed and the proximal jejunum was incised for biopsy,the MLN and celiac vein blood were collected for bacterial culture(aerobic culture).　Results　Group B rat mucosal cell atrophied,DNA and RNA content and plasma cell decreased,the positive rat of MLN bacterial culture was 62.5%.Atrophy in group C alleviated and bacterial translocation rate declined(37.5%).Group D was similar with group A.　Conclusions　GLN combined with EGF is more effective in protecting gut barrier function and preventing bacterial translocation than GLN alone.

By discussion the experience of surgery teaching for foreign students by the methods of CBL+MCAI, the successful teaching experience are the followings: making sufficient preparations before lesson according to the features and difficulties in the foreign students' surgery teaching; Emphasis on CBL teaching methods and rational application of multimedia computer assisted instruction,strengthening the teaching disciphne of foreign students and the cultivation of the spirit of students' active learning.These measures stimulated the enthusiasm of students' active learning and improved the quality of teaching.

Objective: To explore clinical features of acute myocardial infarction (AMI) caused by left main (LM) coronary artery lesions and to study the effect of percutaneous coronary intervention (PCI) in relevant patients. Methods: A total of 3514 AMI patients received coronary angiography (CAG) in our hospital from 2000-01 to 2015-12 were studied, those including 36 of infarct-related artery (IRA) as LM. There were 28/36 patients received PCI and 8 received CABG. The clinical features and outcomes in 28 LM disease patients were investigated. Results: The patients included 5 female and 23 male at the mean age of (66.5±8.32) years. There were 16 patients with ST-segment elevation myocardial infarction (STEMI) and 12 with NSTEMI; 21 received primary PCI and 7 had elective PCI; there were 16 patients suffered from cardiac shock at admission. The procedural success rate was 82.1% and the in-hospital mortality was 35.7% (10/28). During (66.1±35.2) months follow-up period, 3 patients had re-NSTEMI and 1 of them received PCI again, 3 patients died. No event survival rate was 66.7%. Conclusion: PCI is feasible for treating AMI patients caused by LM lesions, the in-hospital survival rate was 64.3%; while the MACE occurrence rate during long-term follow-up period has been high.

Objective To study the relationship between plasmic nitric oxide(NO),endothelin-1(ET-1) levels and occurrence of hepatopulmonary syndrome(HPS) in liver cirrhosis with portal hypertension(LCPH) patients.Methods Fifty-two LCPH patients were divided into group Ⅰ(with HPS) and groupⅡ(without HPS).Plasmic NO and ET-1 levels were detected by nitric acid reductase and radioimmunoassay(RIA) respectively.Results Occurrence of HPS in all patients was 11.5%(6/52),and was 30%(3/10) in Child-Pugh class C patients.In group Ⅰ,50%(3/6) patients were in Child-Pugh class C.The comparison showed that plasmic NO levels were significantly higher in group Ⅰ than that in group Ⅱ[(110.40?28.02)?mol/L vs.(79.56?22.12)?mol/L,P0.05).Conclusions Elevated plasmic NO levels is an important characteristic of HPS,and,when combined with Child-Pugh classification,it can be significant for diagnosis of HPS in LCPH patients.

Objective To investigate the adhesion mechanism of Lactobacillus acidophilus FN001外to Peyer's patches. Methods Adhesion of L. acidophilus FN001 to mice Peyer's patches was studied in vitro using a fluorescent quantization method. The nature of adhesion mediator was studied by the effects of physical, chemical and enzymatic pre-treatments of the bacteria on their adhesion and effect of sugars on in- hibition of adhesion. The presence of lectin-like proteins in the cell surface was determined by hemagglutina- tion. Effect of L. acidophilus FN001 on inhibition of adhesion of pathogens to Peyer's patches was also stud- ied. Results The adhesion of L. acidophilus FN001 was strongly inhibited in the presence of D-mannose and methyl-ct-D-mannoside. Pretreatment of L. acidophilus FN001 with pepsin and trypsin decreased the ad- hesive capacity indicating that cell surface proteins are involved in adhesion to Peyer's patches. L. acidophi- lus FN001 could agglutinate rabbit red cell in mannose specific manner and protease pretreatment could de-crease hemagglutinin, suggesting that L. acidophilus FN001 has mannose specific lectin (s). In adherence inhibition assay, L. acidophilus NF001 could significantly inhibit adhesion of E. coli ATCC25922 to Peyer's patches when L. acidophilus NF001 were applied to Peyer's patches first or at the same time with pathogen. Conclusion It was concluded that a mannose-specific protein mediated adhesion of L. acidophilus FN001 to the Peyer's patches, and L. acidophilus FN001 could inhibit adhesion of pathogen with similar lectins speci- ficity to Peyer's patches.