Carcinoma of urinary bladder is one of the most common tumor.As life rhythm speeding up and lifestyle changing,bladder cancer incident increases year after year.Previous major treatment methods for the disease are open surgery and TURBT,but these therapies can not overcome the characteristics of its recurrence.Since the 21st century along with the rapid development of molecular biology technology,the discovery of Survivin gene,new knowledge about HSP molecules in tumor immunology and the progress of the technique called RNA interference,open up a whole new field for early diagnosis and treatment of bladder carcinoma.Discussing the role of Survivin gene in the development of tumor,survivin antisense RNA' s efficacy and the role of HSP70 in the cancer immunity,the review aims at exploring the possibility of the double gene expression vector constructed by Survivin antisense RNA gene and HSP70 gene for bladder carcinoma therapy.

KLF9(Kruppel-likefactor9)isthetranscriptionfactorthatcanbindGC-richsitesthrough three C2H2-type zinc fingers and can regulate diverse biological processes,including cell proliferation,apopto-sis and the development of the organ.KLF9 is downregulated in some tumor specimens and tumorous cell lines relative to the normal samples and cell lines and plays an important role in the growth of cancer cells.Similar with the other KLF numbers,KLF9 could affect the development of tumors,especially for the endocrine-related cancers through multiple signaling pathways and interventions with other proteins.

The hypoxia tolerance of mice was significantly increased by repetitive action of auto-hypoxia. The tolerance duration of the 2nd, 3rd, 4th and 5th run was 1.8 2.5 3.0 and 3.6 times longer than that of the Ist one. The survival time of mice that had been exposed to hypoxia repeatedly for four runs was 10 times longer than that of the control animals when both of them were placed in the same low pressure chamber and was 4 times longer while KCN was administrated. The survival time under low oxygen pressure in mice injected with brain extract of resistant mice was 1.8 and 2.1 times longer than that of the saline-injected or normal mice's brain extract-injected animals respectively. These results indicate that some plastic or adaptic changes might occur in the tissue cells particularly in the brain cells during acute and repeated hypoxia. They lead the animals' hypoxia tolerance to a very high level. Water soluble antihypoxic or hypoxia-resistant elements might exist in the brain of hypoxia resistant animals, which were extractable, transferable, and permeable to the blood brain barrier.

The present experiment was carried out on mice.The changes in contentsof 12 essential trace elements in the brain of mice were measured by inductivelycoupled plasma emission following acute and repetitive exposure to hypoxia.Followingrepeat exposure to hypoxia for one or two runs, the contents of non-soluble in brainhomogenats as was notably decreased,while that of the soluble V was markedly increased,when compared with that of normal group.When the animals were fed for two daysafter four runs of hypoxia,the contents of As,Fe,Cu,Co,Cr,B,and V were all signi-ficantly or very significantly changed in comparison with the normal value.Further stu-dies are needed to find out the biological importance of these changes and the relationshipbetween them and hypoxia tolerance.

Purpose　To detect the prevention of EGF and GLN on the side effect of TPN.　Methods　We randomized SD rats into four groups:group A (control),B(STPN),C(TPN+GLN),D(TPN+GLN+EGF).All rats were maintained on their respective diets for 8 days,at which time they were killed and the proximal jejunum was incised for biopsy,the MLN and celiac vein blood were collected for bacterial culture(aerobic culture).　Results　Group B rat mucosal cell atrophied,DNA and RNA content and plasma cell decreased,the positive rat of MLN bacterial culture was 62.5%.Atrophy in group C alleviated and bacterial translocation rate declined(37.5%).Group D was similar with group A.　Conclusions　GLN combined with EGF is more effective in protecting gut barrier function and preventing bacterial translocation than GLN alone.

Objective:To study the protective effects of exogenous nucleotides on lipopolysaccharide(LPS)immunnostimulation and its mechanism.Method:Forty healthy Kunming mice were randomly divided into five groups:control group,nucleotides(NT)groups(4h,18h),nucleotides free(NF)groups(4h,18h).Control group and NF groups were fed with nucleotide-free diet.NT groups were fed with nucleotide-supplemented diet(0.25% nucleotides).On D 15,mice were lavaged with physiological saline(control)or LPS,and were killed 4 or 18 h later.Serum,liver,small intestine,and peritoneal macrophage were sampled in germfree state.Results:Hepatic Na+K+-ATPase,intestinal superoxide dismutase(SOD),serum total anti-oxidation ability,peritoneal macrophage-produced interleukin 10(IL-10)were increased,and intestinal malonaldehyde(MDA),serum alanine aminotransferase(ALT),intestinal myeloperoxidase(MPO),peritoneal macrophage-produced interleukin 1(IL-1)were decreased with nucleotides supplement.Conclusion:Exogenous nucleotides can help to maintain oxidation-antioxidation and inflammation-antiinflammation balance,and protect mice from injury under LPS immunostimulation.

Objective:To investigate the antihypertensive effects of mung bean protein,peanut protein and rice protein alcalase hydrolysates with in vitro angiotensin I-converting enzyme(ACE) inhibitory activity in spontaneously hypertensive rats(SHR) . Method:The impact of digestive proteases on ACE inhibitory activity of hydrolysates of peanut,mung bean and rice protein isolates were evaluated under simulated gastrointestinal digestion and their antihypertensive effects were investigated in SHR after single oral administration. Results:All of three kinds of protein hydrolysates showed antihypertensive activities after single oral administration at a dose of 600 mg/kg bw,most potent in mung bean protein while least in peanut protein. There were no significant changes in the heart rate of SHR after oral administration of protein hydrolysates. Conclusion:Mung bean protein,peanut protein and rice protein hydrolysates all showed antihypertensive activity,but their potent inhibitory activities on ACE did not correlate with their antihypertensive activities found in SHR.

Objective: To investigate the effect of zinc sulfate and zinc methionine on growth and their possible regulating mechanism in mice. Method: Ninety male KM mice were randomly divided into three groups. The control group was fed on basal diet containing zinc of 11. 67 mg/kg 10d. The ZnSO4 group and Zn-Met group were fed on the diets supplemented with ZnSO4 or Zn-Met at 30 mg/kg(on the basis of Zn) for 10 d. Initial and final body weight,serum zinc concentration, growth hormone (GH),the levels of growth hormone receptor (GHR) and insulin like growth factor 1 (IGF-1) mRNA were determined. Results: Both ZnSO4 and Zn-Met enhanced body weight and serum zinc concentration of mice,Zn-Met more effectively than ZnSO4 for body weight . Both forms of zinc had no effect on GH and the expression of GHR mRNA , but both up-regulated the expression of IGF-1 mRNA. As compared to ZnSO4, Zn-Met enhanced the level of IGF-1 mRNA significantly. Conclusion: Both ZnSO4 and Zn-Met had no effect on GH and the expression of GHR Mrna,but enhanced the expression of IGF-1 mRNA. Zn-Met enhanced the body weight gain and up-regulated IGF-1 mRNA expression more effectively than ZnSO4.

Objective According to the infrared spectrum of renal stone composition analysis,discuss the relationship between gender,age and kidney stone.Methods Kidney stone composition analysis of 408 cases had been done in our hospital from the 2002 to 2011 by using Fourier transform infrared spectroscopy.There were 271 males (66.4％) and 137 females (33.6％).Results There were 359 cases with calcium stones,accounting for 88.0％ (359/408); 197 cases with calcium oxalate stones alone,accounting for 48.3％; 180 cases with phosphate stones,accounting for 44.1％; 211 cases with mixed composition stones,accounting for 51.7％.Main chemical composition of the stones were:calcium oxalate 48.3％,carbonate apatite 34.8％,urates 5.4％,ammonium magnesium phosphate 4.4％,ammonium carbonate eutectic 3.4％,brushite 1.5％,cystine 2.0％ and protein 0.3％.Male patients accounted for 66.4％ of all cases and the peak onset age was 30-59 years; Female patient accounted for 33.6％ of all cases and the peak onset age was 50-59 years.Conclusions Calcium oxalate and phosphate are the most common components of kidney stones.Males age between 30-59 years and females age between 50-59 years may suffer from of kidney stone formation.

A novel peptide, named BF2-X, was designed based on the structure-activity analysis of an analogue of Buforin II, named BF2-A. The BF2-X was a hybrid peptide containing the N-terminal residues 5 to 13 of BF2-A and three repeats of the C-terminal regular alpha-helical motif RLLR, and the residues 8 valine were replaced by leucine. The results of bioinformatics analysis had showed that compared with BF2-A, the helicity, positive charge, hydrophobicity rate and C-terminal amphipathy of BF2-X had remarkably enhanced. Both peptides showed a random coil structure in an aqueous solution, while displaying a typical alpha-helical structure in 50% trifluoroethanol solution (a membrane mimic condition). BF2-X exhibited higher alpha-helical contents than BF2-A in hydrophobic environment. BF2-X displayed potent antimicrobial activities against a broad spectrum of microorganisms. And BF2-X showed stronger antimicrobial activities against bacteria tested than parent peptide BF2-A. These results suggest that the alpha-helical content was directly correlated with the enhanced antibacterial activity. Both peptides had no hemolytic action on mouse erythrocyte.