Objective To explore the effects of natural killer (NK) T cells activated by ?-galactosylceramide (?-GalCer) on (NZB?NZW) F1 mice (BWF1). Methods Forty BWF1 mice were divided into two groups (5th week group, 6th month group) according to the age when the treatment started. Mice were treated with ?-GalCer or vehicle by intraperitoneal injection of various dosages and therapeutic courses. The survival time and proteinuria were recorded. Mice blood were withdrawn before and every month after injection, and the collected sera were frozen for analysis of IgG anti-dsDNA, anti-cardiophospholipid antibodies (ACL) and rheumatoid factor (RF). Another ten mice′s blood were withdrown 2 h later after ?-GalCer or vehicle iv injection. Cytokines including IL-2, IFN-?, IL-4 and IL-10 were detected. Results Cytokines IL-2 and IFN-? secreted by Th1 were increased while cytokines IL-4 and IL-10 secreted by Th2 decreased in ?-GalCer iv injection mice. The level of IgG anti-dsDNA antibodies and RF increased with age in ?-GalCer treated mice which was lower than those of vehicle treated mice. No difference in survival rate and severe proteinuria (≥3+) rate was observed between ?-GalCer and vehicle treated BWFI mice in the two groups. Conclusion ?-GalCer may activate NKT cells and decrease some autoantibodies. NKT cells activation using ?-GalCer is not enough to improve proteinuria and survival rate.

Bycomparingthe clinical internship ofFrench students in the Alsace Cancer Center of France(Centre Paul Strauss)with Chinese medical students,we found the differences and got some valuable implications.

Objective To get knowledge of the air pollution situation inside the car caused by the automotive components and decorative materials and the major impacting factors, and to provide the basis for the development of the car air pollution control and countermeasures. Methods Thirty-five cars of different types and ages were selected from January to July 2008. The concentration of benzene series compounds, formaldehyde and TVOC was determined, a survey and analysis of air pollution inside the car was conducted. Results The results showed that the air pollution inside the new cars was serious, with the increase of the using time, the concentration of pollutants reduced. The amount of formaldehyde emission inside the cars was related to the age of the car, the formaldehyde pollution inside the new cars was the most serious in one year. The longer the car was closed, the higher the concentration of pollutants would be. Using perfume or smoking inside the car, car washing or painting could increase the TVOC concentration inside the car. However, after purification treatment, the concentration of air pollutants inside the new cars could significantly decrease. Conclusion The air pollution inside the new cars is so serious, for the health of passengers and drivers, the related standards for the control of the air pollution should be set as soon as possible.

Objective To evaluate the effect of enteral nutrition emulsion on the immunologic function and intestinal mucous barrier in diabetic patients. Methods Eighty diabetic patients were randomly divided into con-trol group (n=40) and enteral nutrition group (n=40). The urine lactulose (L) and mannitol (M) levels and the blood immunologic indicators recorded on day 1 and day 8. Results The L/M ratio was significantly lower in enteral nutrition group than in control group on day 1 and day 8 ( P < 0. 05 ). The IgG level was significantly higher in enteral nutrition group than in control group on day 8 ( P = 0. 02 ). Conclusion Enteral nutrition emulsion can decrease the permeability of intestinal mucous membrane and improve the immunologic function in diabetic pa-tients.

Objective To study the expression of hyaluronidase(HAase) and matrix metalloproteinase-9(MMP-9) in the invasion and metastasis of human breast cancer.Methods The expression of HAase and MMP-9 in human breast cancer was detected using tissue homogenate preparing, enzyme-linked immunoadsorbent assay(ELSA),and immunohistochemistry examination.Results ①The levels of HAase were 4.89?2.55,8.03?2.66,and 12.00?3.96 mU/g in grade Ⅰ,Ⅱ,and Ⅲ of breast cancer,respectively(n=10,20,3),with significant differences between each other(P

A case-control study was performed to investigate the relationship between gallbladder cancer(GCA) and gallbladder stones (GS). 43 patients with GCA confirmed by pathology were included, in which 19 were accompanied by GS, 24 patients without. 88 controls were selected from the data for the periodic health checkup with B-ultrasonography(B-US): cholelithiasis were discovered in 6 controls, but 82 were free from GS. The difference between the case and control group was significant(P

Objective To observe the influence of secondary spasmodic torticollis on the balance of hemiplegic stroke survivors and to compare the effects of different treatment protocols.Methods Fifty-six secondary spasmodic torticollis patients after stroke were randomly divided into three groups based on the type of treatment.The botulinum toxin (BTX) group received BTX-A injections and common support treatment plus rehabilitation training (n =19),the medication therapy group received oral baclofen and common support treatment plus rehabilitation training (n =18),and the routine rehabilitation group received only routine rehabilitation therapy (n =19).In addition,19 stroke cases without spasmodic torticollis served as a control group.They also were treated with routine rehabilitative treatment alone.Before and after 2 months of treatment,balance was assessed using Berg's balance scale,and spasmodic torticollis was evaluated using Tsui's scale.Results Before treatment the balance of the control group members was significantly better than that of the other three groups.After 2 months of therapy,balance function had improved significantly in all four groups.The balance of the botulinum toxin group was better than that of the medication and routine rehabilitation groups.After treatment,Tsui scale scores in the medication and botulinum toxin groups were significantly lower than before treatment,and the scores in the botulinum toxin group were significantly lower than in the medication and routine rehabilitation groups.There was no significant difference in Tsui scale scores before and after treatment in the control group.Conclusions Spasmodic torticollis inflaences the recovery of balance function in hemiplegic patients after stroke.Treating the spasmodic torticollis can improve their balance.Treatment with BTX-A combined with common support treatmcnt and rehabilitation training showed significant curativeeffect.

Objective To observe the influence of spasmodic torticollis on the rehabilitation prognoses of patients with neurogenic dysphagia.Methods One hundred and three dysphagic patients were recruited,of which 82 had spasmodic torticollis and 21 did not.The subjects with spasmodic torticollis were divided randomly into a medication therapy group (21 cases) which accepted medication plus swallowing training,a common therapy group (20 cases) accepting common support treatment plus swallowing training,a combined therapy group (20 cases) accepting common support treatment,medication,plus swallowing training as well,and a group of 21 cases accepting only routine rehabilitation therapy ( the routine rehabilitation group).The 21 cases without spasmodic torticollis served as a control group,and they were treated with routine rehabilitative treatment.The swallowing function of all patients was evaluated before and after therapy using videofluoroscopic swallowing study techniques.Results There was no significant difference with regard to the swallowing function among the groups before treatment except that the swallowing function of the control group (without torticollis) was significantly better than that of the other 4 groups.After treatment,swallowing function had improved significantly in all 5 groups.The average improvement in the routine rehabilitation group was,however,significantly less than in the others.After treatment there was no significant difference between the combined therapy group and the no torticollis control group with regard to swallowing function.Conclusions Spasmodic torticollis influences the recovery of swallowing function in dysphagic patients.Therapy for spasmodic torticollis can improve swallowing among dysphagic patients with spasmodic torticollis.

Objective To investigate the change of (NZB?NZW) F1 mice (BWF1) with ? 2 microglobulin deficiency and explore the role of ? 2 microglobulin in murine lupus.Methods ? 2 microglobulin knockout BWF1 mice were made according to the reference.The IgG type anti phospholipid antibodies.IgG type and anti dsDNA antibodies,rheumatoid factor,IgG and IgG3 were detected using ELISA.The survival rate,autoantibodies,IgG and IgG3 were compared between knockout mice (? 2m -/- ),wild type mice (? 2m +/+ ) and heterozygous mice (? 2m +/- ).Results ? 2m deficiency did not affect the survival rate of BWF1 mice.But the mortality of male BWF1 mice was lower than that of famale BWF1 mice.Antiphospholipid antibodies in ? 2m -/- BWF1 mice were lower than the wild type group and heterozygous group at month 4.Anti dsDNA antibodies had no difference between the three groups.RF was lower in ? 2m -/- BWF1 mice than ? 2m +/- and ? 2m +/+ BWF1 mice at month 4 and then elevated.At month 9 was higher than ? 2m +/- and ? 2m +/+ BWF1 mice significantly.As for the IgG,it was lower in ? 2m -/- BWF1 group than in ? 2m +/- and ? 2m +/+ BWF1 mice at months 4 and 6,and had no differnce between three groups.It minght be due to the severe proteinuria,ascites and hypoproteinemia.IgG3 was lower in ? 2m -/- BWF1 mice than in other groups,which only occur at month 6.Conclusion ? 2m knockout affects the murine lupus BWF1 in many ways,but not the survival rate.It affects the generation of autoantibodies mainly the antiphospholipid antibodies and RF.? 2m deficiency affects the function of neonatal FcR and results in IgG decreased because of difficult absorbent of IgG from gut and increased catabolism.

Objective To study the effect of hyaluronidase and VEGF on human breast cancer invasion and metastasis. Methods By means of ELSA and immunohistochemistry, hyaluronidase and VEGF were detected in breast cancer. Results Hyaluronidase were (3.99?1.91) mU/g, (8.01?2.59) mU/g and (12.00?3.96) mU/g in gradeⅠ, Ⅱ, Ⅲ of breast cancer respectively. Hyaluronidase levels were significantly elevated in grade Ⅱ,Ⅲ as compared to grade Ⅰ, Hyaluronidase levels were significantly elevated in grade Ⅲ as compared to grade Ⅱ(P