Objective To study the influence of Mahuang Decoction(MD)in different combinations on diaphoretic function in rats.Methods Vacuole incidence of axillary sweat gland was determined by observing the histolomorphological changes of vacuole to evaluate the diaphoretic function.The diaphoretic function was observed in 30 minutes after administration.Results The combination of Mahuang(Herba Ephedrae)and Guizhi(Ramulus Cinnamomi)had the strongest diaphoretic function in all of the different combinations.The diaphoretic function of the combinations including Mahuang(Herba Ephedrae)was stronger than those excluding Mahuang(Herba Ephedrae).The diaphoretic function of Mahuang(Herba Ephedrae)became stronger when used together with Guizhi(Ramulus Cinnamomi),remained the same when used together with Xingren(Semen Armeniacea Amarum),and became weaker when used together with Gancao(Radix Glycyrrhizac).Conclusion Mahuang(Herba Ephedrae),Guizhi(Ramulus Cinnamomi),Xingren(Semen Armeniacea Amarum),and Gancao(Radix Glycyrrhizac)play the roles of principle drug,assistant drug,adjuvant drug and conductantr drug respectively in Mahuang Decoction,which embodying the method of composing a prescription.

Objective To explore the effects of embolizing intracranial ruptured aneurysm in early stage using Guglielmi Detachable Coil (GDC). Methods 10 intracranial ruptured aneurysms including 8 cases of the first ruptured and 2 cases of the second ruptured were studied. The locations of aneurysm were as following: 1 case in anterior cerebral artery, 1 case in middle cerebral artery, 5 cases in anterior communicating artery, 2 cases in posterior communicating artery, and 1 case of multianeurysm in anterior and posterior communicating artery. Hunt-Hess grade was as following: 2 cases of gradeⅠ,4 cases of gradeⅡ, 2 cases of gradeⅢ, 1 case of grade Ⅳ, and 1 case of grade Ⅴ. Fisher CT scale was as following: 2 cases of gradeⅠ, 6 cases of gradeⅡ,and 2 cases of grade Ⅲ. Embolization of intracranial ruptured aneurysm was performed in early stage using GDC, 7 cases in 3 days after ruptured, and 3 cases in 6 days after ruptured. Results Among 11 aneurysms in 10 cases, 8 cases were 100% embolization, 2 cases≥95%, and 1 case≥90%. Followed-up from 1 month to 2 years after operation 1 case was dead, 7 cases recovered well, 1 case was in vegetable state, and 1 case occurred subarachnoid hemorrhage again. Conclusions GDC early embolization is preferable technique for treating intracranial ruptured aneurysm.

Objective To explore the microneurosurgical treatment of recurrent intra-spinal canal tumors. Methods The clinical data on the microsurgical parameters, operative procedures, operative efficacy and post-operative complications of 16 cases with recurrent intra-spinal canal tumors from June 1993 to June 2002 were reviewed retrospectively. Results Under the operative microscope, the tumors were totally removed in 10 cases, subtotally in 4 cases, and partially in 2 cases. Fllowed up from 5 months to 8 years, 11 cases recovered well, 4 cases suffered from moderate disability, and 1 case died. Preoperative CT or MRI scan, knowledge of the anatomy and structures, and skillful operative techinique all were important to microsurgical treatment of recurrent intra-spinal canal tumors. Conclusion Once diagnosis of recurrent intra-spinal canal tumors, the operative treatment is effective. Radiation therapy for the malignant tumors or incompletely removed recurrent tumors is necessary.

Objectives To summarize experiences and improve efficacy of comprehensive treatment for patients with wound bitten by venomous snakes. Methods Totally, 547 patients with wound bitten by venomous snakes were hospitalized during January 1993 to December 2002, with comprehensive treatment focusing on purified antivenom serum and paying attention to intensive care for the lung, brain, kidney, blood and circulatory function to detect and handle with viscera damage earlier. Large-dose corticosteroid and anisodaminie was used in treatment for snake-bitten patients to improve clinical effects.Results Among 547 patients, 501 were cured (91.6%), 28 (5.1%) improved, and limb dysfunction was left in 8 (1.5%), including two needed skin grafting, fingers or toes amputed in three and one hemiplegia, and ten died with a case-fatality ratio of 1.8%. Conclusions Multiple organ failure caused by venomous snake bite is one of high risk factor leading to death. Comprehensive and symptomatic treatment for snake-bitten wound can reduce its case-fatality.

Objective:To study the effect of airway IL-4RA gene transfer on asthma-associated expression of STAT6 in the mice of allergic asthma.Methods:Forty BALB/c mice were randomly divided into four groups:blank control,asthma model,asthma model administered by pLNC-laz,and asthma model administered by glucocorticoid and pLNC-IL-4RA.The ten mice in each groups were sensitized by i.p.injection of 10 ?g of OVA emulsified in 40 mg of aluminum hydroxide in a total volume of 200 ?l on the days of 1st,7th and 14th.On the 15th day,the mice were challenged via airways with OVA for 7 days,20 min a day,blank control were sensitized by i.p.injection of 200 ?l pH=7.4 PBS on days 7 and 14.Asthma model was administered by pLNC-laz or pLNC-IL-4RA for 3 days beginning at the 12th day.The mice of the group with glucocorticoid was administered with glucocorticoid on the 15th day for 7 days.24 hours after the last challenge,the mice were sacrificed by bloodletting.Serum OVA-specific IgE and blood Eosinophils were measured.The lungs were fixed by immersion in 10% formalin.Expression of STAT6 was identified by immunohistochemistry.Results:It was shown that pLNC-IL-4RA was integrated to the genomic DNA of pneumonic tissues.Retrovirus-mediated delivery of IL-4RA to airways of the asthma mice weakened airway eosinophilia triggered by either IL-13 or IL-4.Furthermore,IL-4RA delivered by retrovirus was expressed in airways of the mice with allergen sensitization,resulting in significiant reduction of expressing level of asthma-associated STAT6 in the experimental mice of allergic asthma.Conclusion:Retrovirus mediated delivery of IL-4RA to airways reduces expression of STAT6 in pneumonic tissues of asthmatic mice.Thus,the gene theraphy can be a potential therapeutic option to treat and control chronic airway inflammation and asthmatic symptoms.

Objective To explore the clinical value ofmulti-slice spiral CT (MSCT) to carcinomatous intestinal obstruction of colon.Methods The clinical and MSCT data of 40 patients with carcinomatous intestinal obstruction of colon diagnosed by MSCT were analyzed.Results In the 40 cases,complete intestinal obstruction were in 17 cases,incomplete intestinal obstruction in 23 cases,39 cases were carcinomatous intestinal obstruction of colon confirmed by surgery and pathology,and 1 case was intussusception intestinal obstruction by multiple polyps.The MSCT qualitative analysis of obstruction reason was 97.5％ (39/40),the MSCT diagnostic accuracy of intestinal obstruction was 100.0％ (40/40).The obstruction site was at ascending colon showed in 14 cases,transverse colon in 4 cases,splenic flexure of colon in 2 cases,descending colon in 6 cases,sigmoid colon in 12 cases,both ascending colon and transverse colon in 1 case,colon and small intestine in 1 case.The obstruction site accuracy rate of MSCT was 100.0％ (40/40).Conclusion MSCT has obvious advantages in obstruction existing,obstruction site,obstruction reason to carcinomatous intestinal obstruction of colon,it can offer reliable examination data to clinic.

Objective To summarize the experience of operation treatment of severe mitral stenosis associated with small left ventricle.Methods The clinical data of 115 patients with severe mitral stenosis received mitral valve replacement (MVR) were retrospectively analyzed.According to whether associated with small left ventricle,they were divided into small left ventricle group (61 cases) and non-small left ventricle group (54 cases).The postoperative early complication rate and mortality rate between two groups were compared.Results The postoperative early complication rate and mortality rate in small left ventricle group was higher than that in non-small left ventricle group [14.75％ (9/61) vs.7.41％ (4/54),55.74％ (34/61) vs.25.93％(14/54)],there was significant difference(P ＜ 0.05).Conclusions The positive and effective perioperative management and correct intraoperative decision is key to reduce the incidence of early postoperative complications and mortality after MVR surgical in patients with severe mitral stenosis associated with small left ventricle.

The paper takes PubMed database as the data source,conducts visualized analysis on the literatures in the field of international diabetes health promotion in recent decade through the bibliometric method,explores the annual literature publication situation in this field,core authors and journals,major research hotspots and existing problems,and indicates that the research mode of diabetes health promotion field is relatively consolidated,there is less development and evaluation of new theories and methods,and less attention is paid to other diabetes health care except for diet and exercise.These research directions are worthy of attention by Chinese researchers in the future.

Objective To explore the application of domestic bladder capacity scanner in intermittent catheterization in patients with neurogenic bladder. Methods 40 patients with neurogenic bladder accepted intermittent catheterization. Their bladder volume was tested with domestic and imported bladder capacity scanner (Padscan and Bladderscan) and catheterization respectively. Results The bladder volume was (262.592±6.3012) ml tested by Padscan, and was (262.570±6.3006) ml by Bladderscan (P>0.05). The bladder volume tested by bladder catheterization was (245.549±6.0511) ml, which was significantly lower than the other methods (P<0.001), but the D-value was just 17 ml. Conclusion The accuracy of bladder volume tested by domestic bladder capacity scanner (Padscan) was very similar with the imported one (Bladderscan), and the difference between them and the volume tested by bladder catheterization was meaningless in clinical.

Objective To investigate the effect of COX￣2 inhibitor celecoxib on radiosensitity of irradiation￣resistant cell line CNE￣2R of nasopharyngeal carcinoma and the potential mechanism. Methods Via exposing to a series of X￣ray (2, 4, 6, 8 Gy, 3 times for each dose), radio￣resistant cell subline CNE￣2R was established from human nasopharyngeal carcinoma cell CNE￣2.Radiosensitivity was detected by clone formation assay.CNE￣2R and CNE￣2 cell lines were exposed to 25, 50, 75 μmol.L-1 celecoxib, respectively.Western blotting was used to detect the protein expression of COX￣2.Clone formation assay was performed to measure the survival fraction of CNE￣2 and CNE￣2R after radiotherapy alone or radiotherapy combined with 30 μmol.L-1 celecoxib treatment.Flow cytometry was used to measure influence of radiotherapy alone or radiotherapy combined with 30 μmol.L￣1celecoxib treatment on cell apoptosis.Number of residual γ￣H2AX foci was observed by immunofluorescence assay. Results The colony forming assay demonstrated that the values of SF2, D0 , Dq , and N of CNE￣2R cell subline [(0.81±0.05), (2.15±0.07) Gy, (2.94±0.08) Gy, (3.91±0.07), respectively] was significant higher than those of CNE￣2 cell line [(0.61±0.08), (1.47±0.06) Gy, (1. 68 ± 0. 10) Gy, (3. 13 ± 0. 05), respectively]. The expression of COX￣2 protein was significantly downregulated with increasing celecoxib concentration.Surviving fraction was decreased in both CNE￣2 and CNE￣2R cell lines after irradiation.After radiotherapy combined with celecoxib, apoptosis rates of CNE￣2 and CNE￣2R cell lines [(13.10±0.63)%, (5.30±0.75)%] were higher than those of the corresponding control groups [(4.90±0.71)%, (1.82±0.82)%].Celecoxib increased radiosensitivity in nasopharyngeal carcinoma CNE￣2R and CNE￣2 cell lines.The number of residual γ￣H2AX foci after irradiation was increased by celecoxib pretreatment.The difference was statistically significant (P<0.05). Conclusion Celecoxib can enhance radiosensitivity of radio￣resistant cell subline CNE￣2R of human nasopharyngeal carcinoma in vitro.