1.Expression of Survivin in Human osteosarcoma and its correlation with PTEN and cyclinD1
Orthopedic Journal of China 2006;0(03):-
[Objective]To investigate the expression of Survivin and its relationship with expression of PTEN and cyclinD1.[Method]Immonohistochemical method(SP method)was used to detect the expression of Survivin,PTEN and cyclinDl in 40 osteosarcoma tissues and 20 ostanchondroma tissues.[Result]The expression rate of Survivin was significantly higher in osteosarcoma(62.5%)than that in osteochondroma(10.0%)(P
2.Role of Matrix Metalloproteinase-9 in Signal Transduction Pathway of Cigarette Smoke-induced Airway Mucous Hypersecretion
Journal of Environment and Health 2007;0(07):-
Objective To determine the involvement of matrix metalloproteinase-9(MMP-9) in the signal transduction pathway of cigarette smoke-induced airway mucous hypersecretion. Methods The well cultured BEAS-2B airway epithelial cells were incubated with different concentrations of cigarette smoke extract (CSE),also some cells were preincubated with MMP-9 inhibitorⅠbefore CSE or exogenous epidermal growth factor(EGF) was added. The mucin(MUC)5AC mRNA level was analyzed by RT-PCR,MMP-9 and the phosphorylated EGFR(p-EGFR) protein production was assayed by western blot,MUC5AC protein and EGF protein were detected by enzyme-linked immunosorbent assay respectively,and gelatin zymography was used to determine the activity of MMP-9. Results Incubation of BEAS-2B cells with CSE up-regulated MUC5AC gene expression and protein production in a dose dependent manner (P0.05). Conclusion CSE stimulates the activity of MMP-9 and MMP-9 protein,causing the shedding of the EGF and leading to EGFR activation. This activation of EGFR downstream signaling pathway increases MUC5AC gene expression and mucin production.
3.Anterior cruciate ligament reconstruction and tendon-bone healing characteristics
Chinese Journal of Tissue Engineering Research 2007;0(37):-
Presently,with anterior cruciate ligament reconstruction using autogeneic hamstring tendon graft is generally developed,the influencing factors and promotion methods of tendon-bone healing become a research focus of sports medicine.The influencing factors include the graft fixation,the character of bone,stress stimulation and the matching relation between graft and bone tunnel and so on.The promotion methods include applying growth factors,metalloprotease blocking agent,osteoprotegerin,mesenchyme stem cells,physical methods.Some prospective methods of promoting tendon-bone healing have been found,but these methods are in animal study or involving complex laboratory technique.Thus,these methods require further studies before clinical application.
4.Comparison of therapeutic effect lowering low density lipoprotein cholesterol in Uygur and Kazak na-tionality patients with coronary heart disease from Xinj iang
Chinese Journal of cardiovascular Rehabilitation Medicine 2014;23(3):292-294
Objective:To compare the level of low density lipoprotein cholesterol (LDL-C)before and after treat-ment between Uygur and Kazak nationality patients diagnosed as coronary heart disease (CHD).Methods:A total of 191 Uygur nationality patients and 184 Kazak nationality patients,who received coronary angiography and were diagnosed as CHD first time, and received measurement of LDL-C concentration.They received CHD secondary preventive treatment for 8~12 months,LDL-C concentration was measured again after treatment,and it's com-pared and analyzed between two nationalities.Results:After treatment,there were significant reductions in levels of total cholesterol (TC)and LDL-C in Uygur and Kazak nationality CHD patients.Compared with Kazak nationality pa-tients,there were significant reductions in levels of LDL-C [(2.74±0.13)mmol/L vs.(2.21±0.17)mmol/L]in Uygur nationality CHD patients (t=3.141,P<0.01).Conclusion:Effect lowering in Uygur nationality patients with coronary heart disease ,especially low density lipoprotein cholesterol is better than that of Kazak nationality.
5.Effects of c-Jun N-terminal Kinase on Expression of Interleukin-10 in Keratinocytes Induced by Ultraviolet A
Li AN ; Qian GAO ; Guoqing DONG
Journal of Environment and Health 1992;0(04):-
Objective To explore the effect of c-Jun N-terminal kinase(JNK)on the expression of interleukin-10(IL-10) in keratinocytes induced by ultraviolet A(UVA).Methods The HaCaT cells in cultured were either sham irradiated(control) or exposured to 2.4 J/cm2 UVA radiation.The cells were collected at 0-48 h after irradiation,and JNK levels in cells were detected with the immunofluorescence.HaCaT cells were treated with SP600125(a JNK inhibitor) before irradiation,then cells and suspended medium were collected at each time-point after irradiation,and the expression of IL-10 mRNA and protein were determined by RT-PCR and ELISA.Results Compared with control cells,irradiated cells had increased levels of phospho-JNK throughout the entire 48 h following irradiation(P
6.Advance in Electrical Stimulation on Neurogenic Bladder Secondary to Supersacral Spinal Cord Injury(review)
Chinese Journal of Rehabilitation Theory and Practice 2010;16(12):1117-1120
Electrical stimulation is a common technology to treat neurogenic bladder dysfunction secondary to supersacral spinal cord injury. This paper reviews the historical development of this treatment and current status.
7.Influence of acute-hypervolemic-hemodilution with 6% medium-molecular weight hydroxycthyl starch on renal function
Guoqing ZHANG ; Yue LI ; Yicong LI
Chinese Journal of Anesthesiology 1994;0(06):-
65 mm Hg and CVP between 10-14 cm H2O. In control group no AHHD was performed. Blood samples and urine specimens were obtained before anesthesia (T1), at the end of operation (T2 ) and 72 h after operation (T3 ) for determination of blood and urinary creatinine (BCr, UCr) , blood sodium (BNa) , urinary sodium (UNa), blood urea nitrogen (BUN) , urinary ?1-microglobulin (?1-MG), urinary albumin, urinary immuno-globulin G, creatinine clearance (CCr), and fractional sodium clearance (FENa) . Results BUN, urinary albumin, urinary immuno-globulin G, CCr and FENa were normal during the study in both groups. Urinary concentration of ?1-MG was significantly increased at T2 as compared with the baseline value before anesthesia (T, ) and declined at T3 but was still significantly higher than that at T1 . There was no significant difference between the two groups in terms of the parameters mentioned above. Conclusion AHHD with 6% HES (200/0.5) has no detrimental effects on renal function in patients with normal function.
8.Protection against hydrogen peroxide-induced cytotoxicity in PC12 cells by guggulsterone
Hongbin XU ; Ling LI ; Guoqing LIU
Acta Pharmaceutica Sinica 2008;43(12):1190-1197
The present study is to investigate the protective actions of guggulsterone against the cytotoxicity produced by exposure to hydrogen peroxide (H2O2) in PC12 cells. It was evaluated by MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl-tetrazolium bromide] reduction assay, lactate dehydrogenase (LDH) release assay, and the release of nitric oxide (NO). ROS and Ca2+ in cells were evaluated by DCFH and Fura 2-AM, respectively. Mitochondrial membrane potential (MMP) was assessed by the retention of rhodamine 123 (Rh 123). Apoptosis and morphological alteration in PC12 cells were monitored with flow cytometry and electric microscope. Vitamin E, a potent antioxidant, was employed as a comparative agent. The results showed that preincubation of PC12 cells with guggulsterone (0.1-10 μmol·L-1) prevented cytotoxicity induced by H2O2. Extracellular accumulation of LDH, NO and intracellular accumulation of ROS, Ca2+ resulting from H2O2 were significantly reduced by guggulsterone. Incubation of cells with H2O2 caused a marked decrease in MMP, which was significantly inhibited by guggulsterone. The percentage of H2O2-induced apoptosis in PC12 cells was 24.3%, and decreased in the presence of guggulsterone (0.1-10 μmol·L-1) by .8.4%, 15 .9%, 11.8%, respectively. Guggulsterone exhibited comparable potency against oxidative stress induced by H2O2 in PC12 cells as that of vitamin E. The present findings showed that guggulsterone attenuated H2O2-induced cytotoxicity, extracellular accumulation of LDH and NO, intracellular accumulation of ROS and Ca2+, loss of MMP, and apoptosis, which may represent the cellular mechanisms for its neuroprotective action.
9.The progress in the proteomics researches of central nervous system diseases and drugs
Guoqing SHENG ; Xiaoping PU ; Changling LI ;
Chinese Pharmacological Bulletin 1986;0(05):-
To elucidate the latest progress of proteomics research of central nervous system diseases and drugs. Proteomics , an important discipline in postgenomic era, is the integrated study of protein properties on a large scale. Currently, the best established application of proteomics are in the clinical and biomedical fields. Proteomics will facilitate the elucidation of network mechanism of the development, progress and prognosis of central nervous system diseases. Proteomics offer a unique opportunity to identify disease specific proteins, accelerate the development of target driven drugs, build up molecular pharmacological models, screen and analysis of the efficacy, toxicity and side effects of potential drugs on a large scale. It is predictable that proteomics will play a tremendous role for the diagnosis, detection and pharmaceutical development of central nervous system diseases.
10.In utero ethanol exposure alters the mitochondrial protein expression of fetal mouse cerebrum
Yajun XU ; Guoqing ZHANG ; Yong LI
Journal of Peking University(Health Sciences) 2003;0(04):-
Objective:To probe the effect of prenatal ethanol exposure on the mitochondrial protein expression in fetal mouse cerebrum. Methods:Pregnant CD-1 mice were given 5.0 g/kg ethanol intragastrically from pregnant days (PD) 6-15. Fetal cerebral mitochondria were isolated on PD 18. The overall mitochondrial protein was applied to two-dimensional gel electrophoresis. Differentially expressed protein spots were cut off and identified by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Activities of respiratory chain complex Ⅳ(72.3%?4.6% of control)and ATP synthase, and ATP content in the fetal cerebral cells were measured. Results:Expressions of some constructional and functional proteins were altered by in utero ethanol exposure. Activities of respiratory chain complex Ⅳ and ATP synthase (80.3%?5.1% of control)were both decreased. ATP content (67.9%?3.9% of control) in the cerebral cells was reduced in the in utero ethanol exposure fetuses. Conclusion:In utero ethanol exposure affects the mitochondrial protein expression of fetal mouse cerebrum, even in the tissue without obvious morphological malformations, which may be a possible mechanism of behavioral defects found in children with prenatal ethanol exposure.