Aim To prepare sterically stabilized liposomes modified with chimeric TNT-3 monoclonal antibody (chTNT-3) and investigate their immunoreactivity and in vitro targeting. Methods An endgroup functionalized polyethylene glycol-lipid derivative (pyridylthiopropionoylamino-polyethylene glycolhydrogenated soy phosphatidylethanolamine, PDP-PEG-HSPE ) was synthesized and incorporated to sterically stabilized liposomes. After mild thiolysis of the PDP groups by dithiothreitol, liposomes were covalently linked with maleimide-derivatized chTNT-3 and formed sterically stabilized immunoliposomes.Coupling efficiency, antibody density, size distribution, immunoreactivity of chTNT-3-modified sterically stabilized liposomes (chTNT-3-SLs) and specific binding properties of the chTNT-3-SLs to fixed Raji cells were determined, separately. Results Higher initial Ab/PDP-PEG-HSPE molar ratios resulted in higher antibody density on the surface of liposomes but lower coupling efficiency. The optimal coupling efficiency of 71% was obtained while antibody density in liposome was 106 μg antibody/μmol phospholipids (as initial antibody/PDP-PEG-HSPE = 1: 10). The chTNT-3-SLs had a narrow size distribution after extrusion and the mean size of this immunoliposomes was (115 ± 33) nm. The immunoreactivity of chTNT-3 can be preserved after efficient attachment of maleimide-derivatized chTNT-3 to the surface of liposomes. But calculated per antibody concentration, the immunoreactivity of chTNT-3-SLs would obviously decrease compared to that of chTNT-3 or chTNT-3 derivatives. Significantly higher binding of chTNT-3-SLs to fixed Raji cells directed by chTNT-3 was obtained compared to other preparations in serial dilutions (P＜0.01). Conclusion chTNT-3-SLs prepared by PDP-PEG-HSPE method remained most immunoreactivity of chTNT-3 and was able to bind nuclear antigens in vitro.

Objective To explore the influence factors and the status of anxiety and depression in main caregivers with cerebral palsy children , improve caregivers psychological health status , and offer references for improving the rehabilitation level of cerebral palsy children .Methods A total of 145 caregivers of cerebral palsy children were selected by cluster sampling to carry on the investigation by using SAS , SDS, WHOQOL-BREF. Results The incidence of anxiety and depression was 42.76%and 53.79%, respectively.The score of anxiety was 47.73 ±10.96, which was higher significantly than domestic routine norm (29.78 ±0.46) with a statistical difference (t =19.730; P <0.01).Anxiety and depression score increased as the life quality score of increased.The single factor analysis results indicated that there was a statistical significance in rate of anxiety among different jobs, education levels,ages,monthly income,and with complications or not (χ2 =11.695, 10.787, 3.919, 5.133, 9.942, respectively; P<0.05), so did the depression among different genders ,and age groups (χ2 =10.393 and 8.919; P<0.05).Multivariate Logistic stepwise regression analysis found the results as following:female caregivers , with complications , and half-paidcombining with New Rural Cooperative Medical System (NRCMS) were easier to have the depressed mood .Workers, peasants, and course of disease above 10 years appeared anxiety easily .Conclusions The caregivers of cerebral palsy children psychological status are relatively poor in hospital , which exists seriously anxious and depression .Medical workers should pay attention to the psychological changes of main caregivers , offering health education and psychological care during the treatment.For there have been mental illness caregivers , it is recommended for them to receive timely psychological treatment , thereby improving the rehabilitation level of cerebral palsy children in an all -round way.