Objective To observe clinical effects of round ligament ventrosuspension or shortening round ligament operation by laparoscopy on treating pelvic congestion syndrome (PCS) after tubal ligation. Methods Thirty patients with PCS were treated in round ligament ventrosuspension or shortening round ligament operation by laparescopy. Follow-up was started from the third month after operative procedure to find out patients' symptoms ameliorated situations. Results Symptoms of all patients were ameliorated in various degrees after operations. The cure rate of the cases was 73.3%(22/30), and the rate of improvement was 20.0% (6/30), and the rate of inefficiency was 6.7% (2/30), and total effectiveness rate was 93.3% (28/30). Conclusions Laparoscopy is chosen to be a routine and first diagnosis or treatment means. Round ligament ventrosuspension or shortening round ligament operation by laparoscopy is a simple, high effective way of treating PCS after tubal ligation.

Objective To explore the expression and significance of matrix metalloproteinase-2 (MMP-2) and basic fibroblast growth factor (bFGF) in human glioma.Methods The expression levels of bFGF and MMP-2 in 56 cases of human glioma and 9 cases of normal brain tissue specimen were detected by immunohistochemistry technique.Furthermore,the correlation between the expression of the bFGF and MMP-2 was compared.Results In normal brain tissue,grade Ⅰ,Ⅱ and grade Ⅲ,Ⅳ,the positive expression of bFGF were (9.51 + 2.69)％,(24.78 ±- 7.57 )％,(41.23 ± 12.35 )％,respectively,and MMP-2were (7.18 ± 2.12)％,(21.56 ± 3.85 )％,(54.32 + 7.32)％,respectively.The differences were significant among them (P ＜0.05).bFGF and MMP-2 were positive correlated (r ＝0.71,P ＜0.01).Conclusions There is an intrinsic correlation between the expression of the bFGF and MMP-2 and histological grade of human gliomas.These findings suggest that the synergistic interaction of bFGF and MMP-2 play an important role during the tumor invasion.

Objective To assess boron intake through food and drinking water for the people living in the boron industrial area.Methods Workers from boron mining and processing plants were selected as the exposure group,the other workers from the area where was near to the boron industrial area were selected as the community control group and peoples from the area where was far from boron industrial area were selected as the background control group,all the subjects were healthy adult men,Jul.2003-Sep.2004.Total 24 h food and drinking water of the subjects were collected,the boron concentrations and daily boron intake were analyzed.Results Boron concentrations of drinking water for the workers from boron mining area,community control group and background control group were 2.05,0.86 and 0.05 mg/L respectively;the food boron concentrations of the three groups were 9.46,6.19 and 3.29 mg/kg(DW)respectively;and daily boron intake through food and water were 8.00,4.25 and 1.40 mg/d respectively.The workers drinking the water from the staff canteen well and having dinner in this canteen had 95.5-469 mg/d boron exposure through food and water with average of 219.0 mg/L which was much higher than that of the other people and even closed to the LOAEL(lowest observed adverse effect level)of animals.Conclusion The risk of boron exposure for workers from boron mining and processing area is much higher than that of the other people.

[Objective]To discuss the spinal single side structure illness to the spinal column stable influence characteristic and the method of treatment,and to analyze the character of comparing the traditional three-column theory with the newly three-pyramidal-column theory.[Method]Through 2001-2006 year our hospital admited preservation integrity spinal single side structure illness 42 cases,among the total single side articular process illness 21 cases,single side vertebral plate illness 10 cases,Single side pedicle of vertebral arch illness 7 cases,single side more structure illness 4 cases.Through conservatively treats 11 cases,Single stage fixed 4 cases,the single stage two-sided fixed surgery treats 27 cases,thus,to analyze and revaluate three-pyramidal-column theory essence.[Result]Forty-two cases obtained the revisit completely,average follow-up for 28 months.Evaluates according to the Shi Shushan 's standard,the excellent 31 cases;the good 8 cases,the fair 3 cases.Good and excellent rate reached above 93%.[Conclusion]The spinal single side structure illness may lead to spinal pyramid tropic or scoliosis,and very little causes spondylolisthesis.The spinal colarnn is a three-pyramidal column lives by many triangles.Any colarnn or the angle being destructed will affect its stability.

[Objective] To study the inhibitory effect of bcl-2 antisense oligonucleotide(ASODN)combined with radiation on lung carcinoma cells NCI-H446.[Method]The cultured NCI-H446 lung carcinoma cells were divided into 5 groups:control,pure radiation,nonsense+radiation,lipofectin+radiation,ASODN+radiation.Every group except for control were assayed by MTT on the inhibition rates,24h,48h,72h after 10Gy irradiation respectively.[Results]The inhibitions of lung carcinoma cells NCI-H446 were observed in ASODN+radiation,lipofectin+radiation,nonsense+radiation and pure radiation groups.The differences between the ASODN+radiation group and the other three groups were significant(P

Traditional Chinese medicine has accumulated rich experience in treating dysfunction of gastrointestinal peristalsis. In recent years, a large number of studies have been made on the mechanism and effects of traditional Chinese medicines on the gastrointestinal peristalsis, and the concept of "gastrointestinal promoting Chinese medicine" has been advocated. These traditional Chinese medicines can be divided into three types: promoting the gastrointestinal peristalsis, inhibiting the gastrointestinal peristalsis, and bi-directional modulating. The in vivo and/or in vitro experiments showed that some of the traditional Chinese medicines for activating blood or regulating qi could promote the stomach peristalsis, and the traditional Chinese medicines for moistening intestines to relieve constipation or invigorating spleen to promote digestion could accelerate the intestinal peristalsis. The mechanism lies in the neuroregulation and gut-peptide regulation. Further research on multi-regulation and of multi-target should be done, for the mechanism of the traditional Chinese medicines in regulating the gastrointestinal peristalsis is far more complicated.

Objective To investigate the changes in kinetic parameters of the major joints of the lower extremity during walking in patients with knee osteoarthritis(KOA),and explore its mechanism for clinical treatment.Methods Sixty patients with KOA in tibia-femoral joint and 30 healthy persons as control group(CON) were recruited.Patients were divided into less severe KOA group(LKOA)and more severe KOA group(MKOA)in accordance to K/L radiological grade,then the kinetic data by 3D-gait analysis system were collect.Results LKOA had more knee maximal abduction movement than MKOA and CON after heel strike(P<0.001);the knee first peak adduction movement of MKOA was significantly greater than LKOA(P<0.05)and CON(P<0.001)during medial stance phase;the knee second peak adduction movement of LKOA was significantly less than CON(P<0.05);the hip first peak adduction movement of MKOA was significantly greater than CON(P<0.05).Conclusion The more severe level of KOA in tibia-femoral joint,the more load on medial compartment of patient's knees was seen,comparing with CON,patients with KOA have greater hip abduction movement and more severe patients have less hip adduction movement.

Objective To observe any rehabilitative effects of individualized exercise training in the treatment of rotator cuff injury in elite table tennis players.Methods Forty table tennis players from the Chinese National Team were studied.Twenty (the experimental group) had rotator cuff injuries and 20 without the injury formed the control group.An individualized rehabilitation treatment protocol was prepared for each of the players in the experimental group and implemented twice weekly for a total of 8 weeks.There was no intervention for the control group.Before and after the treatment,both groups were assessed using a questionnaire for disabilities of the arm,shoulder and hand (DASH),a motion assessment,and BIODEX isokinetic muscle testing.Results There were significant differences in average DASH scores between the two groups,with the experimental group scoring higher than the controls both before and after the treatment.Before the treatment,at angular velocities of 60°/s,180°/s and 300°/s the peak moments of the ER (external rotation,ER) and IR (internal rotation,IR) muscles and the ER/IR ratio were all lower in the experimental group than among the controls.However,after the treatment the performance of the experimental group had improved in all three tests.Conclusion The ER/IR ratio of the dominant shoulder of table tennis players with rotator cuff injury is lower than that of the players without the injury,but individualized rehabilitation treatment can effectively increase the ratio and help to improve their shoulder function.

Objective To investigate the relationship between the incidence of dysphagia and the location of brain lesions in patients with acute stroke.Methods Swallowing function was assessed by Watin drinking water test within 48 hours after admission in 226 cases with acute stroke.The location of the brain lesions was identified by CT and / or MRI.According to the US National Institutes of Health Stroke Scale (NIHSS),evaluation of nerve functions was evaluated within 24 hours after admission in stroke patients.Results The incidence of dysphagia was 50.4％ (114 /226) in 226 patients in acute stage.The degree of neurological deficit of stroke patients on admission was significantly correlated with the occurrence of dysphagia (x2 =13.35,P=0.02).The incidence of dysphagia caused by lesions in internal carotid artery trunk (hemisphere in combination with basal ganglia/internal capsule) was more common in anterior circulating territory(internal carotid artery system),dysphagia with internal carotid arterg(ICA) trunk occlusion (hemisphere in combination with basal ganglia/internal capsule) was more common than that caused by cortex branches occlusion (hemisphere-cortex) or penetrating ICA injuries (basal ganglia/internal capsule) and cortex branches occlusion (hemisphere-cortex),respectively(x2 =8.228,P＜0.05).The incidences of dysphagia induced by brain lesions of different cortex lobes had no statistic differences(x2 =0.312,P＞0.05).In posterior circulating territory (vertebro-basilar system),the incidence of swallowing disorders was significantly higher in brain stem group than in the thalamus and cerebellum (x2 =27.292,P＜0.001).The incidence of the dysphagia caused by lesions in cerebral hemispheres,combined basal ganglia / internal capsule and brain stem lesions were significantly higher than those caused by other lesions(x2 =35.639,P＜0.000).Conclusions Brain lesions of hemisphere combined basal ganglia/internal capsule and brain stem are significantly associated with the occurrence of dysphagia after acute stroke.

Objective To investigate the protein and gene expression of bcl-2, bcl-6 in diffuse large B-cell lymphoma (DLBCL). Methods 73 cases of DLBCL were selected for study using the Envision immunohistochemistry method with a panel of antibodies CD3, CD10, CD20, bcl-6, bcl-2, MUM-1. The bcl-2gene expression in 57 of 73 cases with chromosome translocation t (14; 18), breakage and amplification of 3q27 chromosome in 54 of 73 cases were detected by fluorescence in situ hybridization (FISH) method. Results The percentages of tumor cells expressing CD10, bcl-6, MUM-1, bcl-2 were separately 15.1%, 38.4 %, 71.2 %, 79.2 %. t (14;18) chromosomes were detected in 16 of 57 cases (28.1%). The expression of bcl-2 protein have significantly correlated with immunophenotype subtype (P=0.035), and t (14;18) was significantly correlated with the prognosis (P=0.045). There were no association between the expression of bcl-2 protein and t (14;18)(P=0.710). 11 of 54 cases were presented with 3q27 chromosomal breakage (20.4 %), and 14 cases were chromosomal amplification (25.9 %). The prognosis of cases with positive bcl-6 protein was better than that with negative protein obviously. There was no relationship between bcl-6 and 3q27 chromosomal breakage or amplification (P=1.000). Conclusion The expression of bcl-2, bcl-6 protein and gene were different events and had the different significance on DLBCL. The expression of bcl-2 protein was a prognostic marker correlated with immunophenotype subtype, and GCB type with the positive expression of bcl-2 protein had the poor prognosis. Conversely, t(14;18) was an independent event for the prognosis, and the positive expression have the poor prognosis. Patients who require the target therapy should be detected for the t(14;18). The expression of bcl-6 protein was beneficial to the judgment of DLBCL prognosis, it could be an independent factor of the prognosis. 3q27 chromosomal breakage may be a hint to the poor prognosis.