Objective To observe clinical effects of round ligament ventrosuspension or shortening round ligament operation by laparoscopy on treating pelvic congestion syndrome (PCS) after tubal ligation. Methods Thirty patients with PCS were treated in round ligament ventrosuspension or shortening round ligament operation by laparescopy. Follow-up was started from the third month after operative procedure to find out patients' symptoms ameliorated situations. Results Symptoms of all patients were ameliorated in various degrees after operations. The cure rate of the cases was 73.3%(22/30), and the rate of improvement was 20.0% (6/30), and the rate of inefficiency was 6.7% (2/30), and total effectiveness rate was 93.3% (28/30). Conclusions Laparoscopy is chosen to be a routine and first diagnosis or treatment means. Round ligament ventrosuspension or shortening round ligament operation by laparoscopy is a simple, high effective way of treating PCS after tubal ligation.

Objective To explore the expression and significance of matrix metalloproteinase-2 (MMP-2) and basic fibroblast growth factor (bFGF) in human glioma.Methods The expression levels of bFGF and MMP-2 in 56 cases of human glioma and 9 cases of normal brain tissue specimen were detected by immunohistochemistry technique.Furthermore,the correlation between the expression of the bFGF and MMP-2 was compared.Results In normal brain tissue,grade Ⅰ,Ⅱ and grade Ⅲ,Ⅳ,the positive expression of bFGF were (9.51 + 2.69)％,(24.78 ±- 7.57 )％,(41.23 ± 12.35 )％,respectively,and MMP-2were (7.18 ± 2.12)％,(21.56 ± 3.85 )％,(54.32 + 7.32)％,respectively.The differences were significant among them (P ＜0.05).bFGF and MMP-2 were positive correlated (r ＝0.71,P ＜0.01).Conclusions There is an intrinsic correlation between the expression of the bFGF and MMP-2 and histological grade of human gliomas.These findings suggest that the synergistic interaction of bFGF and MMP-2 play an important role during the tumor invasion.

[Objective] To study the inhibitory effect of bcl-2 antisense oligonucleotide(ASODN)combined with radiation on lung carcinoma cells NCI-H446.[Method]The cultured NCI-H446 lung carcinoma cells were divided into 5 groups:control,pure radiation,nonsense+radiation,lipofectin+radiation,ASODN+radiation.Every group except for control were assayed by MTT on the inhibition rates,24h,48h,72h after 10Gy irradiation respectively.[Results]The inhibitions of lung carcinoma cells NCI-H446 were observed in ASODN+radiation,lipofectin+radiation,nonsense+radiation and pure radiation groups.The differences between the ASODN+radiation group and the other three groups were significant(P

[Objective]To discuss the spinal single side structure illness to the spinal column stable influence characteristic and the method of treatment,and to analyze the character of comparing the traditional three-column theory with the newly three-pyramidal-column theory.[Method]Through 2001-2006 year our hospital admited preservation integrity spinal single side structure illness 42 cases,among the total single side articular process illness 21 cases,single side vertebral plate illness 10 cases,Single side pedicle of vertebral arch illness 7 cases,single side more structure illness 4 cases.Through conservatively treats 11 cases,Single stage fixed 4 cases,the single stage two-sided fixed surgery treats 27 cases,thus,to analyze and revaluate three-pyramidal-column theory essence.[Result]Forty-two cases obtained the revisit completely,average follow-up for 28 months.Evaluates according to the Shi Shushan 's standard,the excellent 31 cases;the good 8 cases,the fair 3 cases.Good and excellent rate reached above 93%.[Conclusion]The spinal single side structure illness may lead to spinal pyramid tropic or scoliosis,and very little causes spondylolisthesis.The spinal colarnn is a three-pyramidal column lives by many triangles.Any colarnn or the angle being destructed will affect its stability.

Objective To assess boron intake through food and drinking water for the people living in the boron industrial area.Methods Workers from boron mining and processing plants were selected as the exposure group,the other workers from the area where was near to the boron industrial area were selected as the community control group and peoples from the area where was far from boron industrial area were selected as the background control group,all the subjects were healthy adult men,Jul.2003-Sep.2004.Total 24 h food and drinking water of the subjects were collected,the boron concentrations and daily boron intake were analyzed.Results Boron concentrations of drinking water for the workers from boron mining area,community control group and background control group were 2.05,0.86 and 0.05 mg/L respectively;the food boron concentrations of the three groups were 9.46,6.19 and 3.29 mg/kg(DW)respectively;and daily boron intake through food and water were 8.00,4.25 and 1.40 mg/d respectively.The workers drinking the water from the staff canteen well and having dinner in this canteen had 95.5-469 mg/d boron exposure through food and water with average of 219.0 mg/L which was much higher than that of the other people and even closed to the LOAEL(lowest observed adverse effect level)of animals.Conclusion The risk of boron exposure for workers from boron mining and processing area is much higher than that of the other people.

Traditional Chinese medicine has accumulated rich experience in treating dysfunction of gastrointestinal peristalsis. In recent years, a large number of studies have been made on the mechanism and effects of traditional Chinese medicines on the gastrointestinal peristalsis, and the concept of "gastrointestinal promoting Chinese medicine" has been advocated. These traditional Chinese medicines can be divided into three types: promoting the gastrointestinal peristalsis, inhibiting the gastrointestinal peristalsis, and bi-directional modulating. The in vivo and/or in vitro experiments showed that some of the traditional Chinese medicines for activating blood or regulating qi could promote the stomach peristalsis, and the traditional Chinese medicines for moistening intestines to relieve constipation or invigorating spleen to promote digestion could accelerate the intestinal peristalsis. The mechanism lies in the neuroregulation and gut-peptide regulation. Further research on multi-regulation and of multi-target should be done, for the mechanism of the traditional Chinese medicines in regulating the gastrointestinal peristalsis is far more complicated.

Objective To investigate the changes in kinetic parameters of the major joints of the lower extremity during walking in patients with knee osteoarthritis(KOA),and explore its mechanism for clinical treatment.Methods Sixty patients with KOA in tibia-femoral joint and 30 healthy persons as control group(CON) were recruited.Patients were divided into less severe KOA group(LKOA)and more severe KOA group(MKOA)in accordance to K/L radiological grade,then the kinetic data by 3D-gait analysis system were collect.Results LKOA had more knee maximal abduction movement than MKOA and CON after heel strike(P<0.001);the knee first peak adduction movement of MKOA was significantly greater than LKOA(P<0.05)and CON(P<0.001)during medial stance phase;the knee second peak adduction movement of LKOA was significantly less than CON(P<0.05);the hip first peak adduction movement of MKOA was significantly greater than CON(P<0.05).Conclusion The more severe level of KOA in tibia-femoral joint,the more load on medial compartment of patient's knees was seen,comparing with CON,patients with KOA have greater hip abduction movement and more severe patients have less hip adduction movement.

Objective To observe the aging,apoptosis,cell cycle arrest and oxidative stress in human skin fibroblast(HSF)induced by UVB,and to detect the expression profiles of p66Shc,a determinant of oxidative stress response and life span,in this process.Methods HSF cells were exposed to UVB at a subcytotoxic dosage twice a day for three days.The cells without exposure served as control.After another 24-hour culture,SA-β-Gal staining was performed to evaluate the senescence state of the cells,flow cytometry to observe cell apoptosis;cell cycle arrest was detected by serum starvation and flow cytometry:ELISA was applied to detect intracellular levels of superoxide dismutase(SOD)and malondialdehvde(MDA),and Western blotting to analyze the expression of p66Shc protein.Results The percentage of cells positive for SA-β-Gal staining increased from 0 to 98.3% after UVB radiation,which strongly suggested an aging state of HSF cells.The percentage of apoptotic cells increased from 0.96% to 37%.and 80.07% of the HSF cells were arrested in G0/G1 phase following the irradiation.Intracellular SOD activity decreased from(52.35±4.97)ng/g to(7.81±0.68)ng/g(P＜0.01).while intracellular MDA was found to increase from(3.52±0.34)ng/g to(33.91±3.20)ng/g(P＜0.05).The p66Shc protein was found to be weakly expressed in HSF in 24 hours following the exposure to UVB,and a stronger expression was noted 48 hours later.Conclusions HSF cells are induced into a state of senescence associated with oxidative stress after UVB irradiation,which may be applied as an in vitro model in aging research.The expression of p66Shc is increased in HSF during this process,and further studies are needed to explore the relation between p66Shc and oxidative stress as well as cellular aging.

ays revealed that the-(167-111)bp region Was the minimal promoter of the human IRF3 gene.These results suggested that transcriptional factors such ag E2F might be involved in the transcriptional regulation of IRF3 gene.

Object To optimize the preparation procedure for Zhixuan Granula (ZXG). Methods The optimum extracting conditions of ZXG were selected by orthogonal test with the active components: 23-acetate alisol B, atractylenolide I, and dried extract as the index, it mice sedation of ZXG was clarified by pharmacodynamics. Results The optimum preparation procedure was as follows: Rhizoma Alismatis and Rhizoma Atractylodis Macrocephalae were extracted with alcohol first, adding 12-fold 70% alcohol by refluxing, extracting twice, 2 h once, then extracted with water, adding 14-fold water, extracting twice, 2 h once. The extract showed the obvious effect on sedation of mice. Conclusion The optimum preparation procedure is reliable, with higher extracting ratio of the active components.