Objective:To investigate the effects of sleep deprivation(SD)on implicit memory.Methods:32 young men were divided into four groups,SD21,SD45,SD69 and Control,8 men each group.Subjects were asked to finish the tests of word completion and words association.Results:After SD,both semantic priming and perceptual priming decreased significantly and the decrease were connected with the length of SD.In the same group,the decrease of perceptual priming of SD groups was significantly lowered than that of semantic priming.In perceptual priming,there was significantly different compared between groups except that of SD45 and SD69.In semantic priming,there was significantly different compared between groups except that of Control and SD21.Conclusions: SD has effect on implicit memory,and it's associated with the length of SD.After SD,there exits dissociation between semantic priming and perceptual priming.Perceptual priming is more affected.

Objective: To investigate the brain mechanisms of sleep deprivation. Methods: Sprague-Dawley rats were sleep deprived by housing them on the small platforms over water. Controls were housed in the normal cage(CC) or on the large platforms over water(TC). The effects of SD on the expression of c-fos protein by immunocytochemistry were investigated. The duration of SD was about 12 hours. They were divided into two groups: early SD group (from 7:00 am) and the late SD group (from 19:00 pm). Results: SD resulted in different expression of c-fos protein at different fields of the brain. The main fields of brain stem were all connected with paradoxical sleep. Compared with the late SD, the early SD group showed higher fos protein expression on suprachiasmatic.Conclusion: The disruption of regular circadian rhythm may affect c-fos protein expression.

The inhibitory effect of Actinidia chinensis Planch(ACP) juice on the endogenous formation of N-nitroso compounds (NOC) in the subjects from a high-risk area for gastric cancer were observed. Total concentration of volatile N-nitrosamines(VNA)in fasting gastric juice and 24-h urinary excretion of N-nitrosoproline (NPRO) were used as indices of endogenous exposure. After iagestion of 30 ml ACP juice the average total VNA concentration was significantly decreased from 2.08?1.06?g/L to 0.42?0.43?g/L (p

Objective To investigate effects of driving fatigue on working memory. Methods The control group included 12 taxi drivers after adequate rest while the fatigue group included 27 taxi drivers who had been driving for 10h. Digit memory span, words span, Digit Subtraction Test, Random Number Generation test were used. Results Compared with the control(6.60 ±0.40,90.67 ±6.65,158.27 ±29.12,0.30 ±0.06,24.29 ±10.59,35.90 ± 10.64 ), driving fatigue group were significantly( P ＜ 0.05 )different in the number of backward number memory span( 5.37 ± 0.72 ), percentage of number of right reaction of the number of total reaction of Digit Subtraction Test(79.95 ± 8.04), total number of RNG( 88.33 ± 19.48 ), RNG(0.40 ± 0.05 ), Coupon( 12.35 ±12.88), NSQ(49.72 ± 8.06). Conclusion Driving fatigue can decline the working memory.

Objective:To study the effect of 30-min nap at three low points of the rhythm during 40-hour sleep deprivation Method:Eight young subjects having normal sleep-wake habits were subjected to nap and total sleep deprivation conditions at an interval of 2 weeks The whole experiment was from 6:00 of the first day to 22:00 totally 40 hours The typing workload was given There were three times of naps, which lasted 30 min every time, at 13:00 (the first day), 1:00 and 13:00 (the second day) Visual event related potentials were measured at 1:00 and the end Symbol recognizing task was given before the experiment, after each nap, at the end of it, and two days after Results:In the symbols recognizing task, whether sleep deprivation with or without naps, the reactive time prolonged, the number of right decreased, the number of lose increased Compared with total sleep deprivation, the reactive time was shortened apparently while the number of right and lose did not change when naps were introduced Whether sleep deprivation with or without naps, the latency of P300 was prolonged, the number of right decreased while the amplitude did not change Compared with total sleep deprivation, the latency was shortened apparently while the number of right and amplitude did not change when having naps Conclusion:Nap has positive effects during sleep deprivation

Objective To investigate effects of driving fatigue on auditory involuntary attention.Method Between-groups design was used.The control group included 13 taxi drivers after adequate rest while the fatigue group included 13 taxi drivers who had been driving for 10 h.Auditory oddball pattern was adopted.The standard stimulus was 800 Hz,probability 80%;target stimulus was 1 000 Hz,probability 10%;novel stimulus was sound generated by computer or other sound,probability 10%.Subjects were asked to press the mouse upon hearing the target sound.Result The distribution of P3a was mainly around the frontal-central area of the subjects in control group;the amplitude of P3a was evidently lowered in subjects after driving fatigue.Conclusion The ability of auditory involuntary attention declines after driving fatigue.

Objective: To study the effect of 30-min nap at three low points of the rhythm during 40-hours sleep deprivation on continuous adding. Methods: Eight young subjects with normal sleep-wake habits were subjected to nap and total sleep deprivation conditions at an interval of 2 weeks. The whole experiment was from 6:00 of the first day to 22:00 of the second day, totally 40 hours. There were three times of nap which lasted 30 min every time, 13:00(the first day), 1:00 and 13:00(the second day). Continuous adding was given separately at the time before experiment,13:00 and 18:00 of the first day, 1:00,6:00,13:00,18:00 and 22:00 of the second day.and 2 days after the experiment;reaction times and wrong numbers were recorded. Results: Whether sleep deprivation with or without naps, the reaction time was prolonged.Compared with total sleep deprivation, the reaction time was shortened significantly at SD24, SD31 and SD40 when naps were introduced. Whether sleep deprivation with or without naps, the number of wrong answers did not change. Conclusion:Nap has positive effects during sleep deprivation.

Through analyzing the children's pre-hospital transport,emergency medicine,intensive care medicine,nursing,professional training and research,we reviewed the current status of construction and issues of pediatric emergency and critical care system,then put forward recommendations of its development.

Objective To establish the method of the GC fingerprints for Ligusticum chuanxiong essential oil and its quality control.Methods The temperature at both feed inlet and detector was 250℃,the carrier gas was nitrogen,and the flow rate was 2 mL/min.GC of the essential oil in ten batches of L.chuanxiong was analyzed by adopting temprature programmer and the fingerprint peaks were identified by GC-MS.Results To set up the GC fingerprints for L.chuanxiong essential oil,13 peaks were marked out altogether and the GC fingerprints were analyzed by similarity analysis.Conclusion The method is of high degree of presion,reproducibility,and stability.The resolving of each component in the essential oil is good.The established fingerprints could be used as one of the quality control index for L.chuanxiong essential oil.

Objective:To investigate the expression of c-Fos protein after total and partial sleep deprivation(SD) of 96 hours.Methods:SD was induced in the male Sprague-Dawley rats by housing them on the small platforms over water.Controls were housed in the normal cage(CC) or on the large platforms over water(TC).The effects of total and partial 96h SD on the expression of c-Fos protein were examined by immunocytochemisty.Results:SD made Fos protein express differently in the different fields of the brain.The expression of Fos protein of partial SD was wider and lighter than of total SD.Conclusion:Compared with total SD,partial SD has lighter stress.