Histopathological and ultrastructural changes of the lungs were observed in various intervals after high-velocity bullet wounds of the two thighs of dogs. It was found that histologically there were microvascular dilatation, congestion, capillary rupture, interstitial and alveolar bleeding, focal atelecta-sis and pulmonary emphysema in the first half hour and the 6th hour after injury. In addition, microvascular embolism and hyaline membrane formation were seen in the 24th and 72nd hour after injury. Under electron microscopy, there were swelling of capillary endothelium, swelling, degeneration and necrosis of type I pneumocytes, shortening and decreasing of the microvilli of type II pneu-mocytes, significant vacuolation of the lamonar bodies, accumulation of leucocytes and platelets in the capillary lumen,and obvious decrease of the granules in the leucocytes. The mechanism of the pulmonary injury accompanying bullet wound of the thighs was discussed.

Objective This study was designed to investigate the infection sources and transmission range of the first brucellosis outbreak in human in Bijie City in order to take specific control measures.Methods Fangzhu Town of Qixingguan District was selected as the investigation site where the first medical case was reported.① Basic conditions investigation:the population and the livestock conditions were investigated;②Specific investigation:remote specific investigation of the first case was made through telephone by using unified specific investigation form;③Epidemic situation in human:based on the rule of informed consent,serum samples of all sheep raising people were collected for examination;④Epidemic situation in livestock:sheep's serum samples were collected by agricultural sector.Rose-bengal plate agglutination test (RBPT) and standard agglutination test (SAT) were used to test the serum samples.Results There were 6 338 households with 28 682 residents in Fangzhu County totally,including 70 households feeding 2 184 herds of sheep.The fast case had attacked on May 10th,2014,who was transferred to the first people's hospital of Yuanan Province on July 17th,2014,and was diagnosed as brucellosis on July 25th,2014.There were 85 serum specimens from livestock feeders tested totally,17 specimens showed SAT positive,10 cases were confirmed,recessive infection were 7 cases,the infection rate was 20.00％ (17/85),the detection rate was 11.76％ (10/85),and the recessive infection rate was 8.24％ (7/85).A total of 339 sheep serum samples were tested,133 specimens showed RBPT positive.The positive rate was 39.23％ (133/ 339).Conclusion Brucellosis epidemic was exisited in Bijie City among human and animals,and the situations of infection is serious.The prevention and control of brucellosis should be publicity and education on the sheep flocks.

Objective To investigate children' s understanding of food,nutrition and its relation to health.Methods Participants were interviewed individually,various experimental tasks were used to explore 5-9 year old children' s spontaneous classification of familiar food and their understanding of food balance.Results Five-year-old children rely more than elders on physical cues.There was significant age difference of their criterion of classification among physical (H_((2))=12.929,P ＜ 0.01),conventional (H_((2))=5.540,P=0.063)land processing criterion(H_((2))=6.076,P＜ 0.05).There was significant SES difference of their choices(X_((1))~2=5.857,P=0.016 ; X_((1))~2=4.510,P=0.034 in two different tasks).Higher SES children tended to choose balanced food(percentage of choosing balanced food in higher SES group was 75.0% ,93.8% ;45.2% ,74.2% in lower SES group).Conclusion The criterion children used is related to their cognitive development and experience.As they become older,more and more children realize the nutritional value of foods.There were a significant age difference in nutrition-balanced food choice ,and children's social economic status influence their performance.

Objective To investigate the current work situation of senior clinical nurses to provide some advices for the nursing management .Methods The self-made questionnaires were adopted to investigate the work situation of 60 senior nurses for conduc-ting the descriptive analysis .Results The senior nurses′ satisfaction degrees to the professional self -identity ,the chief nurse′s scheduling ,the current work environment ,the approval by hospital and the emphasis degree by the department were not high .They thought that the suitable position for them was the office nurse and the second was the responsibility group leader .Whereas ,the un-dertaking key post at present was the responsibility group leader and the second was the office nurse .Conclusion Due to the partic-ularity of nursing work and the physiological and psychological change ,the senior nurses are unsuitable to the busy front -line work ;the senior clinical nurses have the rich clinical experience ,the stronger communicational and organizational coordination abili-ty ,and are more suitable for the posts of the office nurse and health education nurse ,and second is the post of responsibility group leader .

Objective To observe the effects of dexmedetomidine on the perioperative stress response and immunologic function in hepatectomy patients with hepatitis cirrhosis.Methods Forty patients(Child-Pugh A or B),ASA Ⅱ or Ⅲ with hepatitis cirrhosis undergoing hepatectomy were randomly divided into dexmedetomidine group(group D)and control group(group C)with 20 cases in each.Patients in group D were administered 0.5 μg/kg bolus dose of dexmedetomidine over 10 min, followed by 0.4 μg·kg-1 ·h-1 infusion until closing abdomen.And patients in group C were given normal saline by the same way as in group D.Flow cytometry was used to detect peripheral blood T lymphocytes (CD3 + ,CD4 + ,CD8 + ,CD4 +/CD8 + ),NK cells at 30 min before anesthesia(T0 ),imme-diately(T1 ),24 h(T2 )and 48 h(T3 )after surgery.Enzyme-linked immunosorbent assay was used to detect the levels of inflammatory cytokines concentration(IL-2,IL-10)at T0-T3 and stress hormones (Cor,ACTH,ALD)at T0 ,T2 and T3 .The levels of SBP,DBP and CVP at T0 ,immediately with intu-bation(Ta ),T1 and extubation(Tb ).Results Compared to those at T0 ,the levels of CD3 + ,CD4 + , CD8 + ,CD4 +/CD8 + at T1 and T2 were significantly lower in both groups(P <0.05),and the levels in group D were obviously higher than those in group C(P <0.05).The levels in group D were back to preoperative levels at T3 ,but the levels in group C were still lower than those at T0 (P <0.05).The levels of NK cells were higher at T1 in both groups,and the levels in group D was significantly higher than those in group C(P <0.05).Compared to those at group D,the concentration of IL-2 was lower at T1 and T2 ,the concentration of IL-10 was higher at T2 and T3 in group C (P <0.05).The levels of Cor,ALD,ACTH at T2 and T3 were significantly higher than those at T0 in both groups,but those in group D were markedly lower than those in group C (P < 0.05 ).The SBP,DBP and CVP of two groups was no statistically significant difference at T0 ,and SBP,DBP and CVP of the group D at Ta , T1 ,Tb was lower than that of T0 and group C (P <0.05).Conclusion Patients with hepatitis cirrho-sis underwent general anesthesia has certain immune suppression after liver cancer surgery,and con-tinuous intravenous infusion of dexmedetomidine during anesthesia may suppress the perioperative stress reaction effectively and alleviate the inhibition of immunologic function.

Standardization, objectification, and normalization of TCM syndromes are emphasis, difficulty, and hot issue of inheritance and development of TCM.With the technological development of computer and extensive application of multi-disciplinary integration, research methods for the identification of TCM syndrome classification emerge in endlessly. This article analyzed the application status of multivariate statistical analysis in the identification of TCM symdrome classification, summarized application status and existing problems of cluster analysis, PCA and factor analysis, discriminant analysis and Logistic regression analysis, and structure equation model in the identification of TCM symdrome classification, which provided references for further studies on identification methods of TCM symdrome classification.

The recent studies have shown a significant relationship between persistent organichorine pollutants(POCPs) and semen quality. The biological characters and source of POCPs, the effect mechanisms of POCPs on semen quality, sperm structure and function were reviewed in this paper. The epidemiological evidences for the reproductive disturbance of men caused by POCPs were discussed and the research direction in the future was pointed out.

OBJECTIVE To discuss the nosocomial infection risk factors and preventive strategies in human assisted reproductive technology(ART)laboratory.METHODS The nosocomial infection risk factors in ART laboratory were analyzed,and the corresponding measures were set up.RESULTS In ART laboratory,there were nosocomial infection risk factors existing in management system,preventive process,condition of laboratory,technical program and garbage management.Corresponding measures were established to control the risk of nosocomial infection.CONCLUSIONS The nosocomial infection in ART laboratory can effectively be prevented through establishment of perfect control system,strict operation of the rules and improving technical programs.

Objective To establish the quality standard of Zhimiling Suppository (Cortex Phellodendri, Radix Sophorae Flavescentis, Catechu, Borneolum Syntheticum, Alumen). Methods Catechu, Borneolum Syntheticum, Alumen in the preparation were identified by physics and chemstry method. Berberine Hydrochloride was determined by HPLC. Results The standard curve for Berberine Hydrochloride was linear in the range of 0.080 2～0.802 ?g, the average recovery was 100.4%, RSD was 0.85% (n=6). Conclusion The methods are simple, accurate and reproducible.

0.05). Obesity group showed the higher levels of TG,VLDL-C,FIns, TNF-? and FFA than did normal controls (P