1.Preliminary development of scale on work values for college students
Haili YANG ; Peng WANG ; Guopeng CHEN
Chinese Journal of Behavioral Medicine and Brain Science 2010;19(12):1133-1135
Objective To develop the scale on work values for college students. Methods 63 items were determined through reviewing items in related literatures and scales, experts consulting and panel discussion.528 students from 3 universities in Shanghai were enrolled in the study. The data were analyzed through exploratory factor analysis,discrimination analysis,reliability analysis and other statistical analysis methods. Results College students' work values comprises six dimensions: self-value dimension ( including two sub-dimensions: self-esteem dimension and self-realization dimension), social-value dimension, dimension of the working environment, wages and welfare dimension , job stability dimension, and the dimension of social status. 43 items were reserved after exploratory factor analyses. The coefficient of reliability of the 43 items was 0.943, and the item loading of the 43 items was 0. 321 ~ 0. 792. Conclusion The discernment, internal consistency and validity of the scale accord with psychometric demands.
2.Development of vocational interest scale of university students
Jianmei CHEN ; Peng WANG ; Guopeng CHEN
Chinese Journal of Behavioral Medicine and Brain Science 2010;19(11):1043-1045
Objective To develop a Vocational Interest Scale of University Students which fits the contemporary current university students, and it could give directions to university students when they were choosing a vocation. Methods Taking Holland's theory as the guide and taking 65 items of Self-Directed Search (SDS) for reference, then adding 28 items which standed for contemporary current university students' ideas of vocational interest to develop original Vocational Interest Scale of University Students. Results The formal scale included 7 tendency(subscales) and 61 items,7 subscales were technology tendency, administration tendency, business trade tendency ,art tendency ,investigation tendency,transaction tendency. The reliability of the scale was very good, the Cronbach's coefficients and spit-half reliability were over 0.7. The factor analysis abstracted 7 factors and the total variance explained reached 45%. The factor analysis results indicated that the scale had a good structure.Conclusion This scale make vocational interest as measuring object and could be a useful tool to give directions to university students who are choosing some vocation.
3.Personality traits on higher vocational students with mathematics learning difficulties
Yemei WANG ; Chuancan CHEN ; Guopeng CHEN
Chinese Journal of Behavioral Medicine and Brain Science 2012;21(2):171-173
ObjectiveTo explore the personality traits on higher vocational students with mathematics learning difficulties(MD).MethodsAll students of three grades in their first year were divided into MD students ( n =486) and outstanding students ( n =407 ) according to their final math scores.Cattell's 16 PF Questionnaires was used to assess the personality.ResultsStudents with MD got significantly higher records in personality factors of aggressiveness (6.59 ± 1.94 ),excitability ( 7.29 ± 2.24 ),sensitivity ( 5.85 ± 1.98 ),suspicion ( 3.51 ±2.03 ),fantasy(5.76 ± 1.86 ),urbane ( 5.81 ± 1.75 ),tense ( 5.63 ± 2.05 ),adapting and anxiety type ( 4.49 ±2.23 ),introverted and expert-oriented type(7.59 ± 2.43 ),and cowardice and decisive type ( 5.04 ± 1.62 ) than outstanding students(P<0.05) ;however,they got lower records in personality factors of steadiness(5.89 ±2.19),perseverance (4.41 ± 1.98 ),independence ( 3.74 ± 1.77 ),emotional with unruffled alert type ( 5.51 ± 1.81 ),professional achievements (52.72 ± 10.66),growth ability in new environments ( 18.78 ± 3.91 ) (P < 0.05 ).ConclusionHigher vocational students with MD have sensitive,suspicious,nervous,anxious,exoscopic,excited,fantasy personalities,lacking self-control,elasticity,carefulness and constancy.
5.Expression, purification and application of staphylococcal protein A fused to SUMO
Xuehui GAO ; Jiechao YIN ; Guopeng SUN ; Wenfei WANG ; Deshan LI
Chinese Journal of Microbiology and Immunology 2010;30(7):597-602
Objective To clone the full length staphylococcal protein A(SPA) gene from Staphylococcus aureus (ATCC6538), and subsequently study the gene structure and antibody binding ability.Methods The full length and the functional region of the SPA gene were cloned into pHisSUMO vector respectively, and expressed in E. coli. The full length and the functional fragment of the SPA protein were detected for antibody binding ability and stability. The functional fragment of the SPA protein fused with SUMO was coupled to the CNBr-activated agarose for antibody purification from rabbit serum. Results A variant of the full length SPA gene was cloned, which has been submitted to GenBank (the accession number is EU695225). Two fusion proteins had the same antibody binding ability as the untagged SPA protein. However, the formers was more stable than the latter at the tested conditions. SUMO-SPA conjugated-agarose kept high efficiency for antibody binding. Conclusion To our knowledge, the full length SPA gene of S.aureus(ATCC6538) is a novel variant. The SUMO tag can improve the stability of the functional region of the SPA protein without damaging the antibody binding ability. This fusion protein has been used for antibody purification successfully.
6.ASSOCIATION OF HABITUAL CALCIUM INTAKE WITH BONE MASS IN PREMENARCHE CHINESE GIRLS
Ling WANG ; Yuming CHEN ; Guopeng HE ; Xincai XIAO ; Yixiang SU
Acta Nutrimenta Sinica 1956;0(03):-
Objective: To investigate the association of calcium intake with bone mass in premenarche girls. Method: Bone mineral density (BMD) and bone mineral content (BMC) at the whole body, lumbar spine (L1-L4) and left hip of 194 premenarche girls (10?1 years old) were measured by dual-energy X-ray absorptiometry (DEXA). Dietary calcium intakes were assessed by food frequency questionnaire and 3d weighed diet records. Results: Statistically significant positive correlations between daily calcium intake and bone bone mineral density (BMD) were or bone mineral content (BMC) at all studied bone sites were found (P
7.Simulation study of canceling technology for strong clutters in life detector based on radar
Yujie CHEN ; Jianqi WANG ; Guohua LU ; Guopeng ZHANG
Chinese Medical Equipment Journal 2003;0(11):-
Strong clutters are produced by the echo waves from the debris when the life detector based on radar locates the living human subject under the debris, which makes the life detector work abnormally because the amplifier is saturate. A canceling technology for the strong clutters is provided to solve the problems discussed above in this paper. The canceling technology for the strong clutters can provide methods and theories in practice by constructing and simulating the mathematic model in the computer, analyzing and discussing the results.
8.Robotic mitral valve replacement: A single center, medium-long term follow-up of 43 cases
Guopeng LIU ; Changqing GAO ; Ming YANG ; Cangsong XIAO ; Gang WANG ; Jiali WANG ; Yao WANG ; Yue ZHAO
Medical Journal of Chinese People's Liberation Army 2017;42(6):549-552
Objective To summarize the surgical experience gained from robotic mitral valve replacement (MVR), and demonstrate the long-term clinical follow-up results. Methods From Jan. 2007 to Jan. 2015, more than 700 patients underwent various types of robotic cardiac surgery in the Department the authors served in, and of them 43 patients underwent robotic MVR with da Vinci Surgical System (Intuitive Surgical, USA). Among the 43 patients, the average age was 47±11 years (ranged 19-65 years), and sex ratio (female to male) was 0.8:1. Six patients were with heart function of NYHA class Ⅰ, 30 patients were of NYHA class Ⅱ and 7 patients were of NYHA class Ⅲ. The left ventricular ejection fraction (LVEF) were 54%-78% (64.0%±7.1%), and 20 patients had atrial fibrillation on admission, and 35 patients were with rheumatic mitral stenosis (MS). Atrial septal defect (0.7cm in size) co-existed in 1 case and 1 patient had mild aortic regurgitation. Mechanical or bioprosthetic mitral valve was replaced via left atriotomy by using da Vinci robotic surgical system after cardiopulmonary bypass (CPB) set-up. Radiopaque titan clips was employed by Cor-Knot knot-tying device (LSI Solutions, Inc, Victor, NY) to anchor the prosthetic valve. Trans-esophageal echocardiography (TEE) was performed before and after surgery. The operative data were collected and patients were followed up at outpatient clinic regularly up to 6 years. Results All cases were performed successfully with the same surgery. No conversion to median sternotomy or operative mortality occurred. The average operation time was 292±62 minutes (ranged 140-450 minutes) with CPB time of 124±26 minutes and aortic occlusion time of 88±21 minutes. The postoperative mechanical ventilation support time was continued for 15±6 hours, and the average staying length in critical care unit was 4±1 days. No myocardial infarction, ventricular tachycardia or excessive bleeding was complicated. All patients were successfully followed up for a median of 3.5 years (ranged 1 month to 6 years). In the follow-up period, no incidence of death, stroke, re-operation due to prosthetic endocarditis or prosthetic failure was reported. However, 39.5% (n=17) patients still had atrial fibrillation after surgery. Conclusion Robotic MVR is a safe and effective procedure with excellent long term surgical outcome.
9.Septal anterior ventricular exclusion operation for left ventricular anterior aneurysm:effects on left ventricular shape,volume and function by intraoperative transesophageal echocardiography
Yao WANG ; Changqing GAO ; Gang WANG ; Yang WU ; Weihua YE ; Guopeng LIU ; Jiachun LI ; Jiali WANG
Chinese Journal of Ultrasonography 2008;17(8):657-660
Objective To assess the effects of septal anterior ventricular exclusion(SAVE)procedure on left ventricular(LV)shape,volume and function in patients with post-infarction LV anterior aneurysm by intraoperative transesophageal echocardiography(TEE).Methods Twenty patients with LV anterior aneurysm underwent surgical ventrieular restoration(SVR)with the SAVE procedure.Intraoperative TEE was performed before cardiopulmonary bypass(CPB)and after weaning from CPB.LV volume and ejection fraction(EF)was measured using the biplane Simpson's method.LV end-diastolic and end-systolic volumes,indexed by body surface area(EDVI and ESVI,respectively)were calculated.To estimate the shape of LV,end-diastolic sphericity index(SI)of LV was determined.Results Compared with pre- CPB,after SVR,LV shape became more elliptical:SI increased from 0.76±0.04 to 0.84±0.05,P<0.001.LV size became more normal:EDVI decreased from(121.51±16.91)ml/m2 to(60.27±9.93)ml/m2,P<0.001,and ESVI decreased from(85.81±15.02)ml/m2 to (32.44±5.36)ml/m2,P<0.001,respectively.EF was increased significantly:(46.02±3.90)% vs(29.52±6.0)%,P<0.001.Conclusions The SAVE technique is easy to reshape LV to ellipsoid fogln and normal size and the resultant improved configuration may contribute to improving EF for patients with post-infarction LV anterior aneurysm.
10.The long lasting effect of the murine fibroblast growth factor-21 on blood glucose control of diabetic animals.
Jingzhuang ZHAO ; Guopeng SUN ; Xianlong YE ; Jinnan LI ; Guiping REN ; Wenfei WANG ; Mingyao LIU ; Deshan LI
Acta Pharmaceutica Sinica 2013;48(3):352-8
Insulin is the most common medicine used for diabetic patients, unfortunately, its effective time is short, even the long-acting insulin cannot obtain a satisfactory effect. Fibroblast growth factor (FGF)-21 is a recently discovered glucose mediator and expected to be a potential anti-diabetic drug that does not rely on insulin. In this study, db/db mice were used as the type 2 diabetic model to examine whether mFGF-21 has the long-term blood lowering effect on the animal model. The results showed that mFGF-21 could stably maintain the blood glucose at normal level for a long-term in a dose-dependent manner. Administration of mFGF-21 once a day with three doses (0.125, 0.25 and 0.5 mg x kg(-1)) could maintain blood glucose of the model animals at normal level for at least 24 h. Administration of mFGF-21 every two days with the same doses could maintain blood glucose of the model animals at normal level for at least 48 h, although it took longer time for blood glucose to reach to normal level depending on doses used (twenty injections for 0.125 mg x kg(-1) and 0.25 mg x kg(-1) doses, ten injections for 0.5 mg x kg(-1) dose). Surprisingly, the blood glucose of the treated model animals still maintained at normal level for 24 h after the experiment terminated. Glycosylated hemoglobin level of the animals treated with mFGF-21, which represented long-term glucose status, decreased significantly compared to the control group and the insulin group. The results suggest that FGF-21 has potential to become a long-acting and potent anti-diabetic drug.