Objective To appraise the values of combined determination of cTnI,MYO and CK-MB mass in the diagnosis of viral myocarditis.Methods The myocardial injury markers(cTnI,MYO and CK-MB mass)and myocardial enzyme(LDH,AST,CK and ?-HBDH)of 61 patients with viral myocarditis(VMC),74 patients with non-viral myocarditis(NVMC)and 46 healthy subjects were determined,respectively,by microparticle chemiluminescent immunoassay and continuous monitoring assay.Diagnostic efficiency was calculated by matrix decision method.Results The serum levels of cTnI,MYO and CK-MB mass of the patients in VMC group were obviously higher than that of the patients in NVMC group(0.46?0.21 vs 0.06?0.04?g/L,98.7?38.2 vs 39.2?26.8?g/L and 6.1?4.2 vs 2.2?1.7?g/L,respectively)as well as that of healthy subjects(0.46?0.21 vs 0.07?0.04?g/L,98.7?38.2 vs 36.5?24.7?g/L and 6.1?4.2 vs 2.1?1.5?g/L,respectively,P0.05).For preliminary diagnosis of VMC,the SE of MYO is highest,but the SP of cTnI and CK-MB mass were better choice.Restoration of MYO was earliest while cTnI was latest during convalescence of the patients.Conclusion Combined and uninterrupted determination of the myocardial injury markers can provide better diagnostic accuracy.

To investigate the distrbution of cag Ⅰin Helicobacter pylori(Hp) isolated from Chinese patients, and its relationship to gastroduodenal diseases. Fragment in cag Ⅰ was amplified by polymerase chain reaction(PCR) in 107 Hp strains from Chinese patients. Results The amplicom to cag Ⅰ was positive in 93 strains, of which 57 strains came from patients with chronic gastritis, 31 strains from peptic ulcer, and 5 strains from gastric carcinoma.Conclusion Existance of cag Ⅰ of Hp strains was popular in the Chinese population, but no data had proven the relation ship between the existence of cag 1and occurrence of Hp related diseases and inflammatory infiltration in gastric mucosa.

To investigate the distribution of IS605 in Helicobacter pylori(Hp) isolated from Chinese patients, and its relationship to gastroduodenal diseases. The fragment in IS605 was amplified by polymerase chain reaction(PCR) in 107 Hp strains from Chinese patients. Results: The amplicom to IS605 was positive in 47 strains. Detective rates of IS605 in Hp from duodenal ulcer (13.6%) were lower than that from gastritis(52.2%). It suggested that: the detection of IS605 was related to alternation in virulence of Hp.

A vector for a mutant Chinese Helicobacter pylori (Hp) strains knock out pathogenicity island (PAI) was constructed, which was the basis to establish a Chinese Hp mutant deleting pathogenicity island. Genetic engineering techniques such as polymerase chain reaction, plasmid extraction, agarose gel electrophoresis, restriction analysis, ligation, preparation of competence cell and transformation were used to make two cloning fragments containing two end regions of PAI and a selectable chloramphenicol resistance marker between them, and then engineering the recombined fragments into pBluescript plasmid. The result showed that restriction analysis demonstrated that the engineering mutant vector had been recombined. It suggested that we constructed an engineering mutant vector which targeted to delete the PAI in Chinese Hp strains. It will be useful for addressing the role of PAI in the pathogenesis of Hp infection.

Objective To investigate diagnostic significance of detecting p53 protein in cytologic specimens by endoscopic pancreatic duct brushing for pancreatic cancer. Methods p53 protein in cytologic specimens was detected by immunohistochemistry, and the resules were compared with cytologic specimens by HE staining. Results Sensitivity, specificity, and accuracy of HE staining for pancreatic cancer is 53%, 100% and 70% respectively. Sensitivity, specificity, and accuracy of detecting p53 protein for pancreatic cancer is 59%, 100%, 74% respectively. Sensitivity, specificity, and accuracy of HE staining and detecting p53 protein for pancreatic cancer is 71%, 100%, 81% respectively. Conclusions HE staining combined with detecting p53 protein in cytologic specimens by endoscopic pancreatic duct brushing is a useful stool for diagnosis of pancreatic cancer. It is helpful to differentiate benign diseases of pancreas from malignant tumor of pancreas.

0.05). The products of conjunction of cagⅠ and cagⅡ were found only in 5 strains. The detectable rate of continuous cag PAI was much higher in duodenal ulcer than in chronic gastritis ( P

Objective To investigate the clinical, rad iographic and pathological features of intraductal papillary-mucinous tumor of the pancreas(IPMT). Methods The features, imaging manifestation and pathological find ings of 9 patients with IPMT were reviewed. Results There were 6 male and 3 female in this series with ag e ra nging from 37 to 76 years(average 68.4 years). The common complaint was upper ab dominal pain. Most of the tumors located at the head of pancreas. The feature of ERCP findings include dilation of the orifice of the papilla of Vater, swelling of the papilla of Vater, massive mucin production and dilation of main pancreat ic duct. The pathological finding is adenoma or adenocarcinoma. Conclusions IPMT is a kind of pancreatic tumor and its prognosis i s better than pancreatic duct cancer. We should differentiate IPMT from other pa ncreatic tumors.

Objective To investigate the diagnostic value of detecting K ras gene codon 12 mutations in cytologic specimens by endoscopic pancreatic duct brushing for pancreatic cancer. Methods Thirty five patients at Changhai hospital between 1999 and 2001 were enrolled. Cells from pancreatic duct burshings during ERCP were suspended with PBS. DNA of the cells was extracted and K ras gene codon 12 mutations were detected by means of PCR SSCP. Results K ras gene mutation rate of pancreatic cancer was 70%, which was higher than that of chronic pancreatitis (14%, P

Objective To investigate the gastric epithelial cell death and proliferation in water restraint stress (WRS) rats during the processes of pathogenesis of stress ulcer, by means of biochemical and morphological analysis. Methods Apoptotic cells were quantitated in gastric mucosa before or at different time points after WRS by TUNEL techniques, and the morphological changes of cells were observed by transmisson electronic microscope whilst the cell proliferation was detected by immunohistochemical staining for proliferating cell nuclear antigen (PCNA). Results The result revealed that in normal controls, the apoptotic cells were seen only occasionally at the surface epithelium,and the PCNA positive cells were found in the proliferative zone of the gastric glands. In contrast, the apoptotic quantities rose significantly from 2 h after the WRS to 5 h after the termination of WRS, and reached a peak gradually,while PCNA positive cells decreased significantly. At 8～12 h after the WRS ,the apoptotic cells gradually declined but failed to return to normal even at 24 h. The PCNA cells increased to reach a peak and declined at 24 h towards the value observed in the intact rats. Under the transmission electronic microscopy it showed that in contrast to normal mucosal cells, the morphology of apoptotic cells changed with various characteristics. Conclusions The results suggested that the epithelial apoptosis significantly increased, whereas proliferation decreased; the imbalance between both them induced the damage of gastric mucosal integrity. During the initial stage of mucosal recovery from stress lesion, the apoptosis appears to activate the mucosal cell proliferating, a response leading to the recovery of the mucosa from the stress damage.

The pharmacokinetics and pharmacodynamics of famotidine were investigated in 10 healthy male volunteers after single intravenous administration of 20 mg. The blood drug levels were determined by a high performance liquid chromatography. The 1gC of famotidine in plasma vs time curve were found to be twcncompartment open model in healthy volunteers. The terminal half-life averaged 3.16h; the total distribution volume 99.40L; the total plasma clearance 392.12ml/min; the area under the plasma concentration curve 1057.45 h?ng/ml. A mathematic equation describing the whole course of blood drug levels in relation to inhibitory effects on intragastric acid output is as follows: E= 100?C2.60/(C2.60+14.712.60). The constant 14.71 is EC50 (ng/ml), the blood drug concentration producing 50% of maximal pharmacological effects. Prediction of pharmacodynamic effects from blood drug level and vice versa becomes possible by using the mathematic equation.