Objective To determine the change in expression of detrusor myosin heavy chain isoforms,SM1 and SM2,following bladder outlet obstruction (BOO) in rabbits. Methods Fourteen New Zealand white male rabbits were divided into 2 groups:control group (n=7) and obstruction group (n=7).The bladder necks were partially ligated to form BOO animal model in obstruction group.Then 5 weeks after operation the bladders were resected in both groups, and the weight and volume of the bladders were measured. After the detrusor tissue protein was extracted,SM1,SM2 and their mRNA were tested by western blotting and RT-PCR methods. Results The bladder weights of obstruction and control groups were (13.8?4.4) g and (3.7?0.5) g, respectively (P

Objective The purpose of this study was to evaluate computed tomography urography (CTU) and compared with conventional intravenous urography (IVU) in diagnosis of urological disease. Methods One hundred and sixty-five patients with 111 males and 54 females underwent CTU urography including 78 cases underwent IVU were reviewed in this study. There were 12 cases underwent retrograde pyelography. There were 75 cases of nephrolithiasis and ureterolithiasis, 30 cases of ureteropelvic junction obstruction (UPJO), 30 cases of bladder cancer, 15 cases of pyelogenic cyst and parapelvic cyst, 9 cases of megaloureter and 6 cases of cancer in pelvis and ureter. Results The accuracy of diagnosis was 94.5% (156/165) in CTU group and 46.7% (42/90) in IVU group.The accuracy of diagnosis was 100.0% (75/75)of nephrolithiasis and ureterolith in CTU group and 78. 6%(33/42)in IVU group, 90.0% (27/30) of UPJO in CTU group, 100.0% (30/30) of bladder cancer in CTU group and 75.0%(9/12)in IVU group, 80.0%(12/15)of pyelogenic cyst and parapelvic cyst in CTU group, 100.0% (9/9)of megaloureter in CTU group, 100.0% (6/6)of cancer in pelvis and ureter in CT group. The displayed rate of the distal end of stenosis and obstruction was 79.4%(81/102)in CTU group and 25.0%(15/60)in IVU group. The examination time was (18.9±8.4)min in CTU group and (67.1±26.7)min in IVU group. Conclusion The CTU can provide more information than conventional IVU and can replace the IVU as routine examination in most cases.

Induced pluripotent stem cells (iPSCs) are a type of pluripotent stem cells that can be generated from adult somatic cells.They have similar characteristics and function to embryonic stem cells (ESCs).Over the past decade,iPSCs were widely concerned in regenerative medicine and stem cell field.Especially the patient specific iPSCs have several advantages over ESCs,such as convenient source,do not exist immune rejection and ethical issues,even keep certain individual genotype.At present,tremendous progress have been made about the application of iPSCs in a variety of retinal diseases.Here,this article reviews pluripotent stem cell sources of RPE,photoreceptors and retinal ganglion cells and current transplantation strategies,the safety problems and prospects.

Construction of teacher's professional morality is a major issue that matters the development of both teachers themselves and medical colleges,especially under the current circumstances of constructing a harmonious society.This article summarizes the phenomenon of absence in teacher's professional morality in market-oriented economy,elaborates theoretically the necessity of strengthening teacher's professional morality construction in medical colleges,and proposes briefly strategies to strengthen the construction of teacher's professional morality.

Liquichip is a new type of biochip emerging in the 1990s, which is integrated flow cytometry, laser technology, digital signal processing and traditional chemical technology with the greatest features of high-throughput and high-flexibility. Luminex has promoted new products from Luminex 100/200 to Flexmap 3D. This is an introduction to the development, principle, and application of Luminex liquichip.

Radiotherapy is the main treatment method of early lung cancer not undergoing surgery for medical reasons and advanced non-resectable lung cancer. The local regional control of lung cancer requires high-dose irradiation, but this treatment is restricted by normal tissue tolerance. The measurement and control of errors are crucial for safe implementation of lung cancer precision radiotherapy. Lung tumor radiotherapy error sources, the methods to control these errors, and the error development trend are discussed in this review.

Charities ; Cleft Lip ; Cleft Palate ; Humans ; Program Development

Cleft Lip ; therapy ; Cleft Palate ; therapy ; Humans

Objective To observe the ultrastructure changes of detrusor of urinary bladder following partial outlet obstruction. Methods 14 New Zealand white male rabbits were divided into "A" and "B" group.The "A" group rabbits underwent operation to induce bladder outlet obstruction,"B" group rabbits served as controls.The ultrastructures including rough endoplactmic reticulum and mitochondrion of detrusor cell of each bladder were examined after 5 weeks. Results The average area of rough endoplactmic reticulum in the obstructed group was (5.377?2.318) ?m 2 in each unit cytoplasm area (115.28 ?m 2) versus (0.476?0.319) ?m 2 in the controls ( P

Objective To evaluate the outcome of a new modality of operation—tensionfree vaginal tape (TVT) in treatment of female stress urinary incontinence. Methods Thirteen female patients suffering from stress urinary incontinence were treating with TVT operation.All the patients were confirmed to have stable bladder without outlet obstruction via clinical routine and urodynamic examination before operation.TVT tape of Prolene was implanted around mid-urethra via a minimal vaginal incision. Results The mean operation time was (38?7)min and the mean intraoperative bleeding was (45?10)ml.The mean catheterization time was 2 days and mean hospital stay was 4 days.No major bleeding was observed in operation.All patients were cured and the clinical symptoms disappeared after operation except for one patient with post-operative urine retention.This patient was catheterized for 2 weeks and the tape was cut under local anesthesia;then the urine retention disappeared and no incontinence relapsed. Conclusions TVT is a simple,reliable mini-invasive procedure with low incidence of complications.It can be used routinely for treating female stress urinary incontinence.